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Acta Biochimica et Biophysica Sinica 2007年第5期39卷 317-325页

作者： WU WEI~(1,3#), Weihua WANG~(2.), Zhiwei CAO~(3#), Hong YU~3, Xiaojing WANG~1, Jing ZHAO~3, Hao TAN~3, Hao XU~3, Weihong JIANG~(2.), and Yixue LI~(1,3*) 1 Bioinformation Center, key lab of Systems Biology, Shanghai Institutes for Biological {2.s, Chinese Academy of {2.s, Graduate School of the Chinese Academy of {2.s, Shanghai 2.0031, china 2.laboratory of Molecular microbiology, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological {2.s, Chinese Academy of {2.s, Graduate School of the Chinese Academy of {2.s, Shanghai 2.0032. china 3 Shanghai Center for Bioinformation {12., Shanghai 2.02.5, china BioinformationCenter KeyLabofSystemsBiologyShanghaiInstitutesforBiologicalSciencesChineseAcademyofSciencesGraduateSchooloftheChineseAcademyofSciencesShanghai200031China

Species of the genus Streptomyces are major bacteria responsible for producing most natural antibiotics. Streptomyces coelicolor A3(2. and Streptomyces avermitilis were sequenced in 2.02.and 2.03, respectively. Two-component signal transduction systems (TCSs), consisting of a histidine sensor kinase (SK) and a cognate response regulator (RR), form the most common mechanism of transmembrane signal transduction in prokaryotes. TCSs in S. coelicolor A3(2. have been analyzed in detail. Here, we identify and classify the SK and RR of S. avermitilis and compare the TCSs with those of S. coelicolor A3(2. by computational approaches. Phylogenetic analysis of the cognate SK-RR pairs of the two species indicated that the cognate SK-RR pairs fall into four classes according to the distribution of their orthologs in other organisms. In addition to the cognate SK-RR pairs, some potential partners of non-cognate SK-RR were found, including those of unpaired SK and orphan RR and the cross-talk between different components in either strain. Our study provides new clues for further exploration of the molecular regulation mechanism of streptomycetes with industrial importance.

关键词： Streptomyces two-component system cross-talk phylogenetic analysis

THE industrial UTILIZATION OF CHEMICAL MODIFIED AGRICULTURAL RESIDUES

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天津科技大学学报 2004年第A2期19卷 374-376页

作者： FengXu RuncangSun HuaiyuZhan QiqiharUniversity QiqiharHeilongjiangChina StatekeylaboratoryofPulpandPaperEngineering SouthChinaUniversityofTechnologyGuangzhouChina

Various lignocellulosic materials such as wood, agricultural and forest residues has the potential to be valuable substitute for, or complement to, commercial sorbents for removing heavy metal ions or dyes from waste water or spilled oil from inland water or sea. More than 9 million tons of straw pulp are produced annually in china, which account for about 90% of the world’s total straw pulp. However, huge quantity of remain straw is not used as industrial raw material and is burnt in the fields or on the side of road. These resources can be chemical modified such as acetylation. Modified straws have the characteristics of low cost, high capacity, quick uptake, and easy to desorb. This paper reviews the current status of the {2. for modified agricultural residues, which focus on hemicellulose and cellulose. The potential of these natural sorbents in main industry is also indicated.

关键词：工业应用化学改性农业渣滓纤维素木素纤维小麦纸浆

Dynamic Output Feedback H∞ Controller Design for A Class of Continuous-Time Nonlinear Systems

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Dynamic Output Feedback H∞ Controller Design for A Class of...

巴西农业科技创新:体制机制、特征成效、启示借鉴

第2.届中国控制与决策会议

作者： Xiang-Gui Guo,the School of Electrical Engineering, tianjin University of technology Wei-Wei Che the {2. laboratory of Manufacturing industrial Integrated Automation, Shenyang University and Gui-Ming Guan the Chinese People''s Liberation Army in Shenyang Military Area Command of the Engineer

This paper is concerned with the dynamic output feedback H∞ controller design for a class of nonlinear continuous-time systems with nonlinear uncertainties. The resulting design is such that the closed-loop system not only is asymptotically stable, but also meets the prescribed H ∞ norm constraint. What’s worth mentioning is that the paper proposes a new method which combines S-procedure approach to solve the nonlinear uncertainties. A sufficient condition for the existence of such a controller is obtained by using appropriate Lyapunov functional and linear matrix inequality （LMI） technique. In addition, based on this result, a convex LMI-based optimization method is developed to optimize the H∞ performance. A numerical example is provided to demonstrate the effectiveness of the proposed design.

关键词： Nonlinear systems Dynamic output feedback control S-procedure approach Linear matrix inequality (LMI)

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科技导报 2023年第16期41卷 43-54页

作者：陈天金任育锋中国农业科学院国际合作局北京100081 中国农业科学院农业信息研究所北京100081

采用文献调研法、历史脉络梳理法及网络访谈等方式,系统梳理巴西农业科技创新体制机制。研究发现,重视农业科技创新体系规划与整体设计、强调农业科技创新要服务于产业发展需求、注重农业科技创新国际交流与合作等是巴西农业科技创新体... 详细信息

采用文献调研法、历史脉络梳理法及网络访谈等方式,系统梳理巴西农业科技创新体制机制。研究发现,重视农业科技创新体系规划与整体设计、强调农业科技创新要服务于产业发展需求、注重农业科技创新国际交流与合作等是巴西农业科技创新体制机制的基本特征。目前在精准农业、生物经济、生物控制、生物固氮、转基因技术等领域取得了大量成果,推动巴西农业转型发展。借鉴巴西经验,中国可进一步加快区域研究中心与农产品研究所布局与建设、制定“卡脖子”关键技术创新计划、加强农业科技创新成果转化与推广、加快制定全球性农业科技创新战略计划等,逐步健全中国农业科技创新体系,助力2.35年农业农村现代化发展目标的实现。

关键词：农业科技创新巴西农业现代化

Researches on Microbial Degrading technology of Pesticide-Residues and it’s Applications

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首届国际生物经济高层论坛

作者： Shunpeng Li Zhongli Cui Jiandong Jiang (Department of microbiology college of Life {2.s Nanjing Agricultural University The key lab of Microbiological Engineering of Agricultural Environment MOA 2.0095 P.R china)

＜正＞The explosive use of chemical compounds has led to global deterioration of environmental quality and serious health *** pesticides as the major compounds to control pests and diseases have been popularly used world widely in modern agricultural ***,the intensive use of pesticides has also resulted in serious environmental problems because they are either recalcitrant or biodegraded very slowly. Many methods were thought out for remediation these pesticide residues including incineration,landfills and *** to the limitation of physical-chemical remediation methods,bioremediation constitutes an attractive alternative to physicochemical methods of remediation,and bioremediation is considered as the most effective,less expensive and non-secondary pollution method by many biological and environmental scientists.

关键词： Pesticides contamination microbial degradation bioremediation

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底物的分散和溶解对甾体微生物酶反应的影响

微生物学通报 2001年第6期28卷 68-71页

作者：阳葵李晓静冯霞尹强赵海燕天津大学理学院化学系天津300072

甾体的溶解是微生物转化反应的控制步骤。表面活性剂对憎水性物质的增溶作用是具有憎水内核胶团形成的直接结果。随表面活性剂溶液浓度的增加 ,胶团数目增加 ,甾体水溶性增强。复配型分散剂PSE MGE应用于甾体转化体系 ,相对于单组分分散... 详细信息

甾体的溶解是微生物转化反应的控制步骤。表面活性剂对憎水性物质的增溶作用是具有憎水内核胶团形成的直接结果。随表面活性剂溶液浓度的增加 ,胶团数目增加 ,甾体水溶性增强。复配型分散剂PSE MGE应用于甾体转化体系 ,相对于单组分分散剂PSE而言 ,实现同一程度甾体水溶性增强所需的表面活性剂用量减少 ,对菌体生长的负面影响较小 ,有利于转化。超声与分散剂配合使用的效果优于分散剂单独使用的效果 ,且使用较强的超声声强 (1 2. 4W/cm2.)和较短的超声时间 (5min)时效果最佳。

关键词：甾体化合物微生物转化分散剂超声溶解酶底物

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担子菌草菇总RNA的快速抽提方法

微生物学通报 2004年第4期31卷 81-84页

作者：李迅裴建军邵蔚蓝江南大学工业生物技术教育部重点实验室无锡214036 南京师范大学生命科学学院南京210097

针对高等真菌草菇 (Volvariellavolvacea) ,提供一种改进的简易抽提总RNA的方法。纯化的RNA可直接作如下的各种应用 ,如RT PCR、polyA RNA的富集、差异显示、North ern杂交、引物延伸、cDNA合成、基因表达谱芯片和基因表达谱芯片分析。... 详细信息

针对高等真菌草菇 (Volvariellavolvacea) ,提供一种改进的简易抽提总RNA的方法。纯化的RNA可直接作如下的各种应用 ,如RT PCR、polyA RNA的富集、差异显示、North ern杂交、引物延伸、cDNA合成、基因表达谱芯片和基因表达谱芯片分析。我们用此方法成功地从草菇中抽提到总RNA ,A2.60 /A2.80 为 1 7～ 2. 0 ,A2.60 /A2.30 大于 2. 0 ,数值显示RNA的纯度是足够高的 (RNA没有被蛋白或苯酚等污染 )。电泳图上 2.8S∶1 8S比例约为 2.1 ,显示RNA没有被降解。

关键词：担子菌草菇总RNA

自制录像片在医学微生物学实验教学中的重要作用

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山西医科大学学报（基础医学教育版） 2009年第6期11卷 752-754页

作者：石立莹钟启平李梅李晓眠天津医科大学基础医学院微生物学教研室

教学录像是申报精品课程工作的重要组成部分,但是在各大高校申报精品课程的网页上,自制实验录像仍然比较少见。该文结合医学微生物学实验教学现状及存在的问题,阐述了自制微生物学实验录像的优势和对提高本科教学质量的作用,以及自制实... 详细信息

教学录像是申报精品课程工作的重要组成部分,但是在各大高校申报精品课程的网页上,自制实验录像仍然比较少见。该文结合医学微生物学实验教学现状及存在的问题,阐述了自制微生物学实验录像的优势和对提高本科教学质量的作用,以及自制实验录像片需要重点解决的问题等,旨在号召各大院校自己动手制作实验录像片。

关键词：医学微生物学多媒体教学实验教学

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烟草根际厚孢轮枝菌生态效应研究

微生物学通报 2002年第2期29卷 4-8页

作者：祝明亮李天飞夏振远祝明亮张克勤云南烟草科学研究院生物技术重点实验室昆明650106 云南大学省工业微生物发酵工程重点实验室昆明650091

通过盆栽试验研究生防制剂中厚孢轮枝菌在烤烟K32.和云85根际的定殖情况及对根际微生物的影响。结果表明施入生物菌剂2.后厚孢轮枝菌能在烤烟外根际、根表分离到,但在根内不能分离到或分离数极少,在4周、6周时外根际、根表的分离数达... 详细信息

通过盆栽试验研究生防制剂中厚孢轮枝菌在烤烟K32.和云85根际的定殖情况及对根际微生物的影响。结果表明施入生物菌剂2.后厚孢轮枝菌能在烤烟外根际、根表分离到,但在根内不能分离到或分离数极少,在4周、6周时外根际、根表的分离数达到最大,根内分离数仍很少,8周时分离数又减少。制剂处理后烤烟根际细菌、真菌和放线菌数量在2.时明显减少,4周时有所回升,6周和8周时与对照达到平衡。结果表明厚孢轮枝菌可以在烟草外根际、根表定殖,初期对土壤微生物具有一定的抑制作用。

关键词：烟草根际厚孢轮枝菌生态效应生防制剂