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Discovery of immune related factors in Fenneropenaeus chinensis by annotation of ESTs

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Progress in Natural Science:Materials International 2004年第1期14卷 48-55页

作者： SHEN Yaoqing 1,2. , XIANG Jianhai 1*, WANG Bing 1,2. , LI Fuhua 1 and TONG Wei 3(1. Experimental Marine Biology laboratory, institute of Oceanology, {2., Qingdao 2.6071,China 2. Graduate School of the {2., beijing 100039, China 3. Genomics & Bioinformatics institute, {2., beijing 101300, China) chinese Acad Sci Inst Oceanol Expt Marine Biol Lab Qingdao 266071 Peoples R China chinese Acad Sci Grad Sch Beijing 100039 Peoples R China chinese Acad Sci Geonom & Bioinformat Inst Beijing 101300 Peoples R China

A total of 10446 expressed sequence tags (ESTs) are obtained by a large scale sequencing of a cDNA library from cephalothorax of adult Fenneropenaeus chinensis . An EST analysis platform was built up based on local computers and bioinformatic techniques were used to annotate these ESTs in order to promptly find possible functional genes, especially for immune related factors. About 4% of the ESTs show similarity to the coding sequences of such factors, including lectin, serine protease, serpin, lysozyme, etc. These ESTs provide a partial profile of the immune system in F. chinensis and useful information for further study on these genes.

关键词： Fenneropenaeus chinensis bioinformatics ESTs immune factors

A template of rat brain based on fMRI T2. imaging

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Progress in Natural Science:Materials International 2005年第6期15卷 502-506页

作者： HU Zhenghui1, WU Yigen2. WANG Xiaochuan3, WANG Jianzhi3, CHEN Feiyan1 and TANG Xiaowei1*(1. Department of physics and Bio-X laboratory, Zhejiang University, Hangzhou 31002., China 2. {2. of high Energy physics and Key laboratory of nuclear analysis techniques, chinese academy of sciences,beijing 100039, China 3. Department of Pathophysiology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China) Department of physics and Bio-X laboratory Zhejiang University Hangzhou 310027 China institute of high Energy {2. and Key laboratory of nuclear analysis techniques Chinese Academy of Sciences Beijing 100039 China Department of Pathophysiology Tongji Medical College Huazhong University of Science and Technology Wuhan 430030 China

The development of functional magnetic resonance imaging (fMRI) technology has made it possible to carry out functional brain imaging experiments in small animals. Usually, group data is required to form the assessment of population, which can not only increase the sensitivity of the overall experiment, but also allow the generalization of the conclusion to the whole population. In order to average the signals of functional brain images from different subjects, it is necessary to put all the mapping images into the same standard space (template image). However, up to now, most animal brain templates remain unavailable and it must be done by ourselves. In this study, a template image based on the brains of eight male Wistar rats is obtained, and it is successfully used in our present Alzheimer disease (AD)-like rat model studies as template for spatially normalizing images to the same stereotaxical space. The fMRI results processed with statistical parametric mapping (SPM99) software are in agreement with the results from immunohistochemical experiment, which proves that this method is universally applicable to the pathologic models of other small animals and to human brain lesion studies.

关键词： fMRI animal model atlas template normalization T*2.

Zircon and apatite fission track analyses on mineralization ages and tectonic activities of Tuwu-Yandong porphyry copper deposit in northern Xinjiang, China

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Science China Earth sciences 2007年第12期50卷 1787-1795页

作者： YUAN WanMing1,BAO ZengKuan2.DONG JinQuan2.GUO ZhaoJie3 & DENG Jun1 1 State Key laboratory of Geological Processes and Mineral Resources,China University of Geosciences,{2.100083,China 2.laboratory of nuclear analysis techniques,institute of high Energy physics,chinese academy of sciences,beijing 100039,China 3 Department of Geology,Peking University,{2. 100871,China 1. State Key laboratory of Geological Processes and Mineral Resources China University of Geosciences Beijing 100083 China 2. laboratory of nuclear {2. techniques Institute of High Energy Physics Chinese Academy of Sciences Beijing 100039 China 3. Department of Geology Peking University Beijing 100871 China

The mineralization ages reported in the past in the Tuwu-Yandong copper district not only are different,but also fall into the Hercynian *** study has achieved 9 zircon and 7 apatite fission track analysis *** zircon fission track ages range from 158 Ma to 2.9 Ma and the apatite ages are between 64 Ma and 140 *** mineralization accords with the regional tectonics in the copper *** consider that the zircon fission track age could reveal the mineralization age based on annealing zone temperature of 140―300℃ and retention temperature of ～2.0℃ for zircon fission track,and metallogenetic temperature of 12.―350℃ in this ore *** three mineralization epochs have been identified,i.e.,2.9―2.6 Ma,2.2.2.0 Ma and 165―158 Ma,and indicate occurrence of the min-eralization in the Indosinian and Yanshan *** to apatite fission track ages,the three tectonic-mineralizing epochs are 140―132.Ma,109―97 Ma and 64 Ma,which means age at about 100℃ after the *** three epochs lasted 146 Ma,108 Ma and about 100 Ma from ～2.0℃ to ～100℃ and trend decrease from early to *** is shown by the fission track modeling that this district underwent three stages of geological thermal histories,stable in Cretaceous and cooling both before Cretaceous and after 2. Ma.

关键词： mineralization age, tectonic epoch, fission track, zircon, apatite, Tuwu-Yandong, Xinjiang

Molecular cloning of growth hormone receptor (GHR) from common carp(Cyprinus carpio L.)and identification of its two forms of mRNA transcripts

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Progress in Natural Science:Materials International 2006年第11期16卷 1156-1163页

作者： SUN Xiaofeng~ 1,2., GUO Qionglin~1**, HU Wei~1, WANG Yaping~1 and ZHU Zuoyan~1(1. State Key laboratory of Freshwater Ecology and Biotechnology, institute of Hydrobiology, {2., Wuhan 430072. China 2. Graduate School of {2., beijing 100039, China) [a]State key laboratory of Freshwater Ecology and Biotechnology Institute of Hydrobiology Chinese Academy of Sciences Wuhan China China [b]Graduate School of chinese academy of sciences Beijing China

The cDNA of growth hormone receptor (GHR) was cloned from the liver of 2.year common carp (Cyprinus carpio L.) by reverse transcription-polymerase chain reaction (RT-PCR) and rapid amplification of cDNA end (RACE). Its open reading frame (ORF) of 1806 nucleotides is translated into a putative peptide of 602.amino acids, including an extracellular ligand-binding domain of 2.4 amino acids (aa), a single transmembrane domain of 2. aa and an intracellular signal-transduction domain of 334 aa. Sequence analysis indicated that common carp GHR is highly homologous to goldfish (Carassius auratus) GHR at both gene and protein levels. Using a pair of gene-specific primers, a GHR fragment was amplified from the cDNA of 2.year common carp, a 2.4 bp product was identified in liver and a 32. bp product in other tissues. The sequencing of the products and the partial genomic DNA indicated that the difference in product size was the result of a 97 bp intron that alternatively spliced. In addition, the 32. bp fragment could be amplified from all the tissues of 4-month common carp including liver, demonstrating the occurrence of the alternative splicing of this intron during the development of common carp. Moreover, a semi-quantitative RT-PCR was performed to analyze the expression level of GHR in tissues of 2.year common carp and 4-month common carp. The result revealed that in the tissues of gill, thymus and brain, the expression level of GHR in 2.year common carp was significantly lower than that of 4-month common carp.

关键词： growth hormone receptor (GHR) molecular cloning transcript expression analysis common carp.

A 12.bit multichannel ADC for pixel detectors in particle physics and {2. imaging

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Science China(Technological sciences) 2010年第5期53卷 1208-1214页

作者： WEI Wei1,2.3,WANG Zheng1,3 & ZHAO JingWei1,3 1 institute of high Energy {2.,chinese academy of sciences,beijing 100049,China 2.Graduate University of chinese academy of sciences,beijing 100049,China 3 Key Lab of nuclear Detection Technology and nuclear Electronics of chinese academy of sciences,beijing 100049,China 1. institute of high Energy {2. Chinese Academy of Sciences Beijing 100049 China2. Graduate University of Chinese Academy of Sciences Beijing 100049 China3. Key Lab of Nuclear Detection Technology and Nuclear Electronics of Chinese Academy of Sciences Beijing 100049 China

Modern pixel detectors in nuclear and particle physics experiments and also in nuclear imaging,starve for highly integrated application specified integrated circuit(ASIC),whereas in China the study of ASIC still stays far away from practical *** lack of ASIC strictly limits the research and development of domestic high energy physics field.A 12.bit multichannel ADC designed for high density readout is introduced as a major candidate for solution.A precise model is discussed and the simulation fully agrees with the model,which indicates a key principle of *** is performed according to the given rule,and novel layout techniques are carried *** results in all aspects are also obtained,showing an excellent real performance,which satisfies the practical requirement.

关键词： application specified integrated circuit high density readout Wilkinson ADC multi-channel ADC

Parentage determination of an isolated Yangtze finless porpoise population Neophocaena phocaenoides asiaeorientalis in the Yangtze Tian-e-Zhou Baiji National Natural Reserve based on molecular data

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Progress in Natural Science:Materials International 2005年第2期15卷 54-61页

作者： XIA Junhong 1,2. ZHENG Jinsong 1,2. XU Limei 1,2.and WANG Ding1*(1. institute of Hydrobiology, chinese academy of sciences, Wuhan 430072. China 2. Graduate School of the chinese academy of sciences, beijing 100039, China) institute of Hydrobiology Chinese Academy of Sciences Wuhan 430072 China. Graduate School of the chinese academy of sciences Beijing 100039 China

Reproductive behaviors are poorly known for the Yangtze finless porpoise Neophocaena phocaenoides asiaeorientalis. In this study, the parentage of an isolated Yangtze finless porpoise population inhabiting the Yangtze Tian-e-Zhou Baiji National Natural Reserve is determined by analysis of microsatellite loci and mitochondrial DNA (mtDNA) control region sequences, and the porpoise's reproductive behaviors are studied. Overall 4 full parentage assignments and additional 3 single parentage assignments were determined for the population of 2. individuals. The analysis shows that their estimated reproductive cycle is shorter than that reported previously and there probably exists an overlapping between gestation and lactation period. The study also shows that the female does not show fidelity to a particular male for breeding and vice versa, the oldest males did not monopolize mating and the dominance rank could not be so strict for the porpoise society. Moreover, the porpoise's mating pattern and relatedness among candidate parents are discussed here. These results provide important information for making guidelines of management and conservation for this protected population.

关键词： Yangtze finless porpoise isolated population reproductive behavior parentage

Assessing the Carrying Capacity of Tourist Resorts: An Application of Tourists’ Spatial Behavior Simulator Based on GIS and Multi-Agent System

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Wuhan University Journal of Natural sciences 2005年第4期10卷 779-784页

作者： ZHANG Ren-jun 1,2.1. institute of Mountain Hazards and Environment, chinese academy of sciences, Chengdu 610041, Sichuan, China 2. Graduated School of the chinese academy of sciences, beijing 100039, China chinese academy of sciences Institute of Mountain Hazards and Environment Chengdu Sichuan China (GRID:grid.9227.e) (ISNI:***) Graduated School of the chinese academy of sciences Beijing China (GRID:grid.9227.e) (ISNI:***)

Based on the study of visitors' individual spatial behaviors, a tourists'spatial behavior simulator (TSBS) to assess the carrying capacity of tourist resorts was developed,TSBS employs GIS (Geographic Information System) to manage the spatial data,and Multi-Agent systemto simulate the actions of individual visitors. By utilizing TSBS, visitors' travel patterns such aslocation, cost, and state can be analyzed and predicted. Based on this analysis and prediction, themodel of assessing the carrying capacity of resorts is built. Our results show that TSBS will be aneffective tool to accurately assess the carrying capacity of tourist resorts.

关键词： tourist resort carrying capacity TSBS GIS Multi-Agent simulator

Correlation of Behavior Changes and BOLD Signal in Alzheimer-like Rat Model

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Acta Biochimica et Biophysica Sinica 2004年第12期36卷 803-810页

作者： Zheng-Hui HU#, Xiao-Chuan WANG1#, Li-Yun LI3, Mai-Li LIU3, Rong LIU1, Zhiqun LING1, Qing TIAN1, Xiao-Wei TANG, Yi-Gen WU2., and Jian-Zhi WANG1* Department of physics and Bio-X disciplinary laboratory, Zhejiang University, Hangzhou 31002.,China 1 Department of Pathophysiology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China 2.2. of high Energy physics and Key laboratory of nuclear analysis techniques, chinese academy of sciences, P.O. Box 918, beijing 100039, China 3Wuhan {2. of physics and Mathematics, State Key laboratory of Magnetic Resonance and Atomic and Molecular physics, chinese academy of sciences, Wuhan 430071, China Department of physics and Bio-X disciplinary laboratory Zhejiang UniversityHangzhou 310027China Department of Pathophysiology Tongji Medical College Huazhong University of Science and TechnologyWuhan 430030 China Wuhan institute of physics and Mathematics State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics Chinese Academy of SciencesWuhan 430071 China institute of high Energy {2. and Key laboratory of nuclear analysis techniques Chinese Academy of SciencesP.O. Box 918 Beijing 100039 China

To explore a potential means for the early diagnosis of Alzheimer disease, we studied the relationship of resting T2. signal and tau hyperphosphorylation/spatial memory deficit. The rat model with tau hyperphosphorylation and spatial memory deficit was established by bilateral hippocampi injection of isoproterenol (IP). Then, the correlative alteration between resting T2. signal and spatial memory retention was assessed with blood oxygenation level dependent (BOLD) functional magnetic resonance imaging (fMRI) study and Morris Water Maze test, and Western blot was employed to confirm tau hyperphosphorylation. The analysis showed following results. (1) Tau phosphorylation at Ser396/Ser404 and Ser199/Ser2.2.was signifi- cantly increased in IP-injected rats as detected by PHF-1 and tau-1, respectively. (2. An AD-like spatial memory retention disturbance was induced at 2. h after isoproterenol injection. (3) A sensitivity threshold of resting T2. signal intensity, which separated the IP-treated rats from vehicle control, was obtained by applying linear regression analysis, and an estimated sensitivity statistical threshold was at 32.62. These results suggest that resting T2. signal may serve as a noninvasive quantitative marker in predicting AD-like spatial memory deficits and tau hyperphosphorylation.

关键词： Alzheimer disease (AD) functional magnetic resonance imaging (fMRI) isoproterenol (IP) Morris Water Maze diagnosis

IMAGING IN high ENERGY ASTRONOMY

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空间科学学报 1998年第S1期18卷 12-20页

作者： LI Ti-pei (institute of high Enerpy {2., The chinese academy of sciences, beijing 100039)

Space research is usually costly, and this restricts participation of developing countries. With traditional techniques one must construct high resolution instrument,more and more complicated and expensive, to obtain high resolution results from space observations. It is a difficult task to make observations with both high sensitivity and high resolution. Applying advanced inversion technique, one can subtract more information from old data in public domain,and, furthermore, conduct high sensitive and high-resolution experiments with low-cost low-resolution instruments. So developing and applying data analysis technique is of great importance for developing countries to do basic space *** this paper we summarize efforts of chinese scientists in recent years along this direction on imaging in space high energy astronomy.

关键词： Space astronomy Astronomical satellite Imaging