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Hunan Agricultural Science ＆ Technology Newsletter 2003年第3期4卷 10-12页

作者： Ding Yi College of agronomy and life Science, Southwest Agricultural university, chongqing 400716, china College of agronomy and life Science Southwest Agricultural University Chongqing 400716 China

The experiment was conducted with 180 one-day-old AA broilers that were randomly divided into one control group and one trial group to study the effects of AsN1980 phytase on broilers growth performance and P utilization in low available phosphorus diets. Results showed that phytase supplement decreased F/W by 12.4%-13.4%and increased phosphorus availability by 7.63%-8.27%, both of which were significantly superior to the control group (P< 0.05).

关键词： phytase broiler phosphorus

Phosphorus availability in two soils amended with poultry litter

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Journal of Environmental sciences 1996年第4期8卷 483-490页

作者： Han Xingguo (institute of Botany,Chinese Academy of sciences,Beijing 100093,china)Duane C.Wolf (Department of agronomy,university of Arkansas,Fayetteville, AR 72701,USA) institute of Botany Chinese Academy of Sciences Beijing 100093 China Department of agronomy University of Arkansas Fayetteville AR 72701 United States

Understanding P transformation in soils amended with poultry litter is important if water quality is to be *** objectives were to determine the influence of method of litter application and temperature on P availability. Poultry litter containing 20. 8g P/kg (dry weight) was either surface-applied or incorporated into Captina (fine-silty, siliceous, mesic Typic Fragiudult) and Nixa (loamy-skeletal,siliceous,Glossic Fragiudult) silt loams at rates of 0 or 10g/kg and incubated at 20℃ or 35℃ at a water potential of -40 kpa. Water soluble and available P (0.03 mol/L NH4F+0. 025mol/L HCl extraction) were determined during a 60d laboratory study. Results indicated that water soluble and available P levels in the soils initially decreased, then rapidly increased,and approached a steady state phase after approximately 20d. After 60d, water soluble P levels were significantly higher when litter was surface applied than when litter was incor porated. When the incubation was terminated, the net increase in available P in both soils was>100 mg P/*** efficient recycling of P and protection of water quality, application method and temperature should be considered when amending soils with poultry litter,

关键词： nutrient cycling phosphorus availability poultry litter water quality.

BAF Complex Is Closely Related to and Interacts with NF1/CTF and RNA Polymerase Ⅱ in Gene Transcriptional Activation

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Acta Biochimica et Biophysica Sinica 2005年第7期37卷 440-446页

作者： Li-Hui ZHAO~(1,2) Xue-Qing BA~1 Xiao-Guang WANG~3 Xiao-Juan ZHU~1 Li WANG~1 Xian-Lu ZENG~1 1 institute of Genetics and Cytology,Northeast Normal university,Changchun 130024,china 2 College of agronomy,Jilin Agricultural university,Changchun 130118,china 3 Department of Biology,Changchun Normal College,Changchun 130032,china institute of Genetics and Cytology Northeast Normal UniversityChangchun 130024 China College of agronomy Jilin Agricultural UniversityChangchun 130118 China Department of Biology Changchun Normal CollegeChangchun 130032 China

Brg-or hBrm-associated factor (BAF) complexes,a chromatin-remodeling complex family ofmammalian cells,facilitate transcriptional activity by remodeling nucleosome ***1 is the coresubunit of Brg-associated factor *** the present study,we investigated the spatial relationshipbetween Brg1 and nuclear factor 1 (NF1/CTF) and RNA polymerase Ⅱ (RNAP Ⅱ) upon gene transcriptionalactivation in vivo by employing immuno-gold *** data showed that Brg1 was closely co-localizedwith NF1/CTF and RNAP Ⅱ in HeLa ***,the co-immunoprecipitation assay further revealedthat Brg1 can be isolated together with NF1/CTF and RNAP Ⅱ in the ConA-stimulated,but not the resting,T *** combined results suggested that BAF complexes can interact with NF1/CTF and RNAPⅡ,and this interaction is closely dependent on the activation of gene transcription.

关键词： BAF complex NF1/CTF RNAP Ⅱ gene transcription

Migration and population genetics of the grain aphid Macrosiphum miscanti (Takahashi) in relation to the geographic distance and gene flow

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Progress in Natural Science:Materials International 2005年第11期15卷 1000-1004页

作者： GUO Wei, SHEN Zuorui, LI Zhihong and GAO Lingwang (College of agronomy and Biotechnology, china Agricultural university, Beijing 100094, china) College of agronomy and Biotechnology China Agricultural University Beijing 100094 China

The population genetics of the grain aphid Macrosiphum miscanti (Takahashi) is analyzed by microsatellite markers. Samples collected from 15 locations in china have been examined at 5 polymorphic microsatellite loci. Overall, genetic diversity displays a relation between the migration and gene flow in the grain aphid; a free and frequent gene flow is found in the eastern populations, and gene isolation occurs in the two western populations, especially Datong population and Guiyang population. The natural barriers may present an insurmountable obstacle preventing gene flow and aphid migration. However, a spatial genetic differentiation between populations is correlated with their geographical separation, indicating the geographic differentiation may play an important role in shaping the genetic structure of M. miscanti populations. In addition, most populations of grain aphids are out of Hardy-Weinberg equilibrium and there is het-erozygote deficit. Based on F statistics, the average genetic differentiation among different geographical populations is relatively low. It seems that the long distance migration of the grain aphid may enhance gene flow and decrease genetic differentiation among different populations .

关键词： Macrosiphum miscanti microsatellite gene flow migration.

Inducement of chromosome translocation with small alien segments by irradiating mature female gametes of the whole arm translocation line

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Science china(life sciences) 2008年第4期51卷 346-352页

作者： CHEN ShengWei1,2, CHEN PeiDu1 & WANG XiuE1 1 National Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural university, Nanjing 210095, china 2 College of agronomy and Biotechnology, Yunnan Agricultural university, Kunming 650201, china National Key Laboratory of Crop Genetics and Germplasm Enhancement Nanjing Agricultural University Nanjing China College of agronomy and Biotechnology Yunnan Agricultural University Kunming China

Haynaldia villosa Schur. (syn. Dasypyrum villosum Candargy, 2n=14, VV) has been proved to be an important genetic resource for wheat improvement. The development of translocation with small alien chromosome segments, especially interstitial translocation, will be helpful for better utilization of its useful genes. Up to now, most of the reported Triticum aestivum – H. villosa translocation lines are involved in a whole arm or large alien fragments. In this paper, we report a highly efficient approach for the creation of small chromosome segment translocation lines. Before flowering, the female gametes of wheat-H. villosa 6VS/6AL translocation line were irradiated by 60CO-γ ray at 160 Rad/M dosage rate and three dosages (1600, 1920, 2240 Rad). Anthers were removed from the irradiated florets on the same day and the florets were pollinated with normal fresh pollens of T. aestivum cv. Chinese Spring after 2-3 days. Genomic in situ hybridization (GISH) at mitosis metaphase of root-tip cell of M1 plants was used to detect the chromosome structural changes involving 6VS of H. villosa. Among the 534 M1 plants screened, 97 plants contained small segment chromosome structural changes of 6VS, including 80 interstitial translocation chromosomes, 57 terminal translocation chromosomes and 55 deletion chromosomes. For the 2240 Rad dosage treatment, the inducement frequencies of interstitial translo-cation, terminal translocation and deletion were 21.02%, 14.01%, and 14.65%, respectively, which were much higher than those previously reported. The M2 seeds were obtained by backcrossing of 74 M1 plants involving 146 chromosomes structural changes of 6VS, and it was found that the structural aberrations in the M1 plants could be transmitted to their progenies. Irradiating mature female gametes of whole arm translocation is a new and highly efficient approach for creation of small segment chromosome struc-tural changes, especially for interstitial translocations.

关键词： irradiation mature female gametes small fragment translocation chromosome wheat Haynaldia villosa genomic in situ hybridization

Effects of Partially N-acetylated Chitosans to Elicit Resistance Reaction on Brassica napus L.

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Agricultural sciences in china 2002年第5期1卷 516-519页

作者： ZHANG Xue-kun, TANG Zhang-lin, CHEN Li, GUO Yi-hong, CHEN Yun-ping and LI Jia-na(agronomy Department, Southwest Agricultural university , chongqing 400716 , china) agronomy Department Southwest Agricultural University Chongqing 400716 China

The effects to elicit resistance reaction on oilseed rape (Brassica napus L. cv Xinongchangjiao ) by four partially N-acetylated chitosan 7B, 8B, 9B and 10B (Degree of acetylation (D. A.) is 30% , 20% , 10%, 0%, respectively) and Glycol chitosan (GC, D.A. is 0%) were investigated and compared. Results showed that chitosan were similar to salicylic acid (SA), and could induce resistance reaction, but the reaction was influenced by the degree of acetylation of chitosan. Fully deacetylated chitosans, 10B and GC, elicited chitinase activity, but partially acetylated chitosan, 7B, 8B and 9B, inhibited chitinase activity. Phenyalanine ammonia-lyase (PAL) was also elicited. Elicitor activity increased with on increasing degree of acetylation, 7B induced highest PAL activity among all chitosans. All chitosans induced peroxidase (POD) in a similar level. After elicited by glycol chitosan, like SA treatment, the seedlings increased disease resistance to Sclerotinia sclerotiorum significantly.

关键词： Brassica napus L. Chitosan Degree of acetylation Resistance reaction

Effects of Chinese Milk Vetch (Astragalus sinicus L.) Residue Incorporation on CH4 and N2O Emission from a Double-Rice Paddy Soil

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Journal of Integrative Agriculture 2012年第9期11卷 1537-1544页

作者： ZHU Bo1,YI Li-xia1, HU Yue-gao1, ZENG Zhao-hai1, TANG Hai-ming2, YANG Guang-li2 and XIAO Xiao-ping2 1 College of agronomy and Biotechnology, china Agricultural university, Beijing 100193, P.R.china 2 Hunan Soil and Fertilizer institute, Changsha 410125, P.R.china College of agronomy and Biotechnology China Agricultural University Beijing 100193 P.R. China Hunan Soil and Fertilizer institute Changsha 410125 P.R. China

Methane (CH 4 ) and nitrous oxide (N 2 O) emissions from paddy soils have seldom been estimated when leguminous green manure is applied as a nitrogen source. In this paper, gas fluxes were measured by using a pot sampling device combined with a static chamber method to estimate the effects of Chinese milk vetch (Astragalus sinicus L., CMV) on CH 4 and N 2 O emissions and their integrated global warming potentials (GWP) in a double-rice cropping system. Four treatments (no nitrogen fertilizer, NF; urea as chemical fertilizer, CF; CMV incorporation, MV; 50% CMV incorporation and 50% urea, MVCF) were established. CH 4 flux peaked on the 15th d after treatment application. Total season CH 4 emission was increased by MV and MVCF by 370 and 209%, 152 and 66%, when compared with NF and CF, respectively. Most of the increased CH 4 was emitted in the first two months after incorporation of CMV. N 2 O emission from CF was 17- and 5.6-fold higher than that from MV and MVCF, respectively. Application of CMV restricted N 2 O emission caused by the application of urea. Improved CMV residue management was needed to minify CH 4 emission induced by the input of organic material. Despite the highest GWP being found in MV, we recommend CMV, when applied as an N source in paddy fields, as a potential mitigation tool for greenhouse gas emissions.

关键词： Chinese milk vetch double-rice global warming potential methane nitrous oxide

Construction of Genetic Linkage Map and QTL Mapping for Fiber Quality in Upland Cotton

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棉花学报 2008年第S1期20卷 20-页

作者： ZHANG Zheng-sheng(College of agronomy & Biotechnology,Southwest university,chongqing 400716,china) College of agronomy & Biotechnology Southwest UniversityChongqing 400716China

A comprehensive genetic linkage map was constructed using 270 F2:7 recombinant inbred lines from a cross between two upland cotton cultivars Yumian 1 and *** linkage map comprised

关键词： QTL QTLs Map

Studies of Multi-Allelic Polymorphism of Dominant Dwarfing Genes in Wheat

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Agricultural sciences in china 2003年第12期2卷 1302-1313页

作者： FU Da-xiong, RUAN Ren-wu, YIN Jia-min, ZONG Xue-feng, DAI Xiu-mei, ZHANG Jian-kui, LI Ke, QU Jie and WANG Yi-min (College of agronomy and life Science, Southwest Agricultural university, chongqing 400716, P.R.china) College of agronomy and life Science Southwest Agricultural University Chongqing 400716 P. R. China

Dwarfing breeding of wheat in the world is confined to the exploitation of recessive dwarfing sources. None of the dominant dwarfing sources discovered in common wheat (Triticum aestivum L.) has found wide exploitation in wheat breeding due to the extreme dwarfness of their plants (2055 cm). We found in our work that some stable mutant lines with their plant height enhanced to different extents could be obtained in large populations derived from the stock seeds of the dominant dwarfing sources Aibian1 carrying Rht10 on 4DS and being 2055 cm tall and Aisu2 carrying Rht3 on 4BS and being 55 cm tall, or from their descendants of induced mutation treatments, or from the segregating descendants of their crosses with mid- or tall-statured genotypes. Subsequently, we studied these mutation-derived lines differing in plant height with near isogenic lines and observed that the character of their enhanced plant height bred true, each carrying a semi-dominant dwarfing gene for a definite height and that as the plant height of the mutation-derived lines increased, the yield-contributing characters of their near isogenic lines were significantly improved. When test crosses with marker genes and physiological and biochemical genetic marker tests were performed to re-localize the semi-dominant dwarfing genes carried by the mutation-derived lines, it was confirmed that they shared common loci with Rht10 and Rht3 and that they were all mutation-derived multiple alleles. It is thus speculated that dominant dwarfing genes are of 'multi-allelic polymorphism'. In other words, dominant dwarfing genes, which are ultra-dwarfing, are liable to develop by mutation into a group of multiple alleles with plant height enhanced to different extents and some may have a height close to the ideal plant height for wheat breeding. Therefore, these results offer a fundamentally new approach for the exploitation of dominant dwarfing sources in wheat breeding.

关键词： Wheat Dominant dwarfing sources Mutation Multiple alleles Dwarfing breeding