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genes & Diseases 2024年第4期11卷 234-245页

作者： Li Qi Guiqing Li Peipei Li Hongwei Wang Xiaolong Fang Tongchuan he Jingjing Li Affiliated Hospital of Weifang Medical university School of Clinical MedicineWeifang Medical UniversityWeifangShandong 262700China Jinming Yu Academician Workstation of Oncology Affiliated Hospital of Weifang Medical UniversityWeifangShandong 262700China Decording Therapeutics Corp Shangha 200000China Yangkun Biogroup Co. LtdNanjingJiangsu 210002China The university of Chicago ChicagoIL 60290USA

genetic mutations in TP53 contribute to human malignancies through various *** date,there have been a variety of therapeutic strategies targeting p53,including gene therapy to restore normal p53 function,mutant p53 rescue,inhibiting the MDM2-p53 interaction,p53-based vaccines,and a number of other *** review focuses on the functions of TP53 and discusses the aberrant roles of mutant p53 in various types of *** human p53 adenovirus,trademarked as Gendicine,which is the first anti-tumor gene therapy drug,has made tremendous progress in cancer gene *** herein discuss the biological mechanisms by which Gendicine exerts its effects and describe the clinical re-sponses reported in clinical ***,the clinical studies suggest that the combination of Gendicine with chemotherapy and/or radiotherapy may produce more pronounced efficacy in slowing tumor growth and progression than gene therapy/chemotherapy ***,we summarize the methods of administration of recombinant human p53 adenovirus for different cancer types to provide a reference for future clinical trials.

关键词： Gendicine gene therapy p53 mutation Recombinant p53 adenovirus TP53

BAF Complex Is Closely Related to and Interacts with NF1/CTF and RNA Polymerase Ⅱ in gene Transcriptional Activation

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Acta Biochimica et Biophysica Sinica 2005年第7期37卷 440-446页

作者： Li-Hui ZHAO~(1,2) Xue-Qing BA~1 Xiao-Guang WANG~3 Xiao-Juan ZHU~1 Li WANG~1 Xian-Lu ZENG~1 1 institute of genetics and Cytology,Northeast normal university,Changchun 130024,china 2 College of Agronomy,jilin Agricultural university,Changchun 130118,china 3 Department of Biology,Changchun normal College,Changchun 130032,china instituteofgeneticsandCytology NortheastNormalUniversityChangchun130024China

Brg-or hBrm-associated factor (BAF) complexes,a chromatin-remodeling complex family ofmammalian cells,facilitate transcriptional activity by remodeling nucleosome ***1 is the coresubunit of Brg-associated factor *** the present study,we investigated the spatial relationshipbetween Brg1 and nuclear factor 1 (NF1/CTF) and RNA polymerase Ⅱ (RNAP Ⅱ) upon gene transcriptionalactivation in vivo by employing immuno-gold *** data showed that Brg1 was closely co-localizedwith NF1/CTF and RNAP Ⅱ in heLa ***,the co-immunoprecipitation assay further revealedthat Brg1 can be isolated together with NF1/CTF and RNAP Ⅱ in the ConA-stimulated,but not the resting,T *** combined results suggested that BAF complexes can interact with NF1/CTF and RNAPⅡ,and this interaction is closely dependent on the activation of gene transcription.

关键词： BAF complex NF1/CTF RNAP Ⅱ gene transcription

The GhACS1 gene encoding an acyl-CoA synthetase is essential for normal microsporogenesis in early anther development of cotton

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The GhACS1 gene encoding an acyl-CoA synthetase is essential...

Molecular cloning,characterization and expression analysis of Sox9a and Foxl2 genes in half-smooth tongue sole(Cynoglossussemilaevis)

2007中国植物生理学会全国学术会议

作者： Wang Xiu-Lan Li Xue-Bao (College of Life Sciences,HuaZhong normal university,Wuhan 430079,china)

＜正＞Microsporogenesis,associated with the functional expression of many genes,is a highly programmed and regulated process in flowering *** investigate the roles of the genes during anther development,two anther-specific cDNAs（designated GhACS1 and GhACS2）encoding acyl-CoA synthetases were isolated from cotton（Gossypium hirsutum）flower cDNA ***,the corresponding GhACS1 gene

关键词： Cotton Acyl-CoA synthetase Microsporogenesis gene expression Fatty acid metabolism

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Acta Oceanologica Sinica 2011年第1期30卷 68-77页

作者： DONG Xiaoli CheN Songlin JI Xiangshan SHAO Changwei College of Fisheries Ocean University of China Qingdao 266003 China Key Laboratory for Sustainable Utilization of Marine Fisheries Resources of Ministry ofAgriculture Yellow Sea Fisheries Research Institute Chinese Academy of Fishery SciencesQingdao 266071 China College of Animal Science and technology Shandong Agricultural University Taian 271018 China

Two transcription factors, Sox9a and Foxl2 were cloned from half-smooth tongue sole （Cynoglossus semilaevis）. Sox9a is a new duplication of *** Sox9 gene. The complete cDNA of Sox9a gene was 1 842 bp long coding for 487 amino acids and Foxl2 gene was 1 817 bp coding for 308 amino acids. Sox9a was expressed higher in male brain, pituitary and gonad and Foxl2 were higher in female brain, pituitary and gonad. The expression of Sox9a gene in gonads of neo-males was higher than that of normal females. Sox9a and Foxl2 were expressed higher in gastrula stage than in other stages. In the period of sex differentiation, the expression of Sox9a was first going up and then going down and Foxl2 was higher expressed at 37 dph. The highest expressions of Sox9a and Foxl2 genes occurred in nine-month and 12-month old gonad tissues, respectively. Sox9a gene was considered to have inevitable links with sex reversal, sex differentiation and cell differentiation of embryos and formation of spermatogenic ceils. Foxl2 was considered to play a role in sex differentiation, cell differentiation of embryos but not to be necessary for sex determination and sex reversal.

关键词： half-smooth tongue sole Cynoglossus semilaevis Sox9 Foxl2 real-time PCR sexreversal neo-male

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LOD score exclusion analyses for candidate disease susceptibility genes using case-parents design

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Progress in Natural Science:Materials International 2006年第1期16卷 30-38页

作者： DENG Hongwen1,2,3 and GAO Guimin2,4 (1.Laboratory of Molecular and Statistical genetics, College of Life Sciences, Hunan normal university, Changsha 410081, china 2.Osteoporosis Research Center, Creighton university Medical Center, Omaha NE 68131, USA 3. Department of Biomedical Sciences, Creighton university, Omaha NE 68131, USA 4. Virginia Bioin format ics institute and Department of Statistics, Virginia Polytechnic institute and State university, Blacksburg VA 24061-0315, USA) [a]Laboratory of Molecular and Statistical genetics College of Life Sciences Human National University Changsha China [b]Osteoporosis Research Center Creighton University Medical Center Omaha NE USA [c]Department of Biomedical Sciences Creighton University Omaha NE USA [d]Virginia Bioinformatics institute and Department of Statistics Virginia Polytechnic Institute and State University Blacksburg VA USA

The focus of almost all the association studies of candidate genes is to test for their importance. We recently developed a LOD score approach that can be used to test against the importance of candidate genes for complex diseases and quantitative traits in random samples. As a complementary method to regular association analyses, our LOD score approach is powerful but still affected by the population admixture, though it is more conservative. To control the confounding effect of population heterogeneity, we develop here a LOD score exclusion analysis using case-parents design, the basic design of the transmission disequilibrium test (TDT) approach that is immune to population admixture. In the analysis, specific genetic effects and inheritance models at candidate genes can be analyzed and if a LOD score is ≤ - 2. 0, the locus can be excluded from having an effect larger than that specified. Simulations show that this approach has reasonable power to exclude a candidate gene having small genetic effects if it is not a disease susceptibility locus (DSL) with sample size often employed in TDT studies. Similar to association analyses with the TDT in nuclear families, our exclusion analyses are generally not affected by population admixture. The exclusion analyses may be implemented to rule out candidate genes with no or minor genetic effects as supplemental analyses for the TDT. The utility of the approach is illustrated with an application to test the importance of vitamin D receptor (VDR) gene underlying the differential risk to osteoporosis.

关键词： association studies candidate genes case-parents design disease susceptibility locus exclusion mapping LOD score.

Brassinosteroid signal trans-duction: An emerging picture

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Chinese Science Bulletin 2003年第18期48卷 1906-1912页

作者： WANG Qiaomei1 & MA Ligeng2 1. Department of Horticultural Science, Zhejiang university, Hangzhou 310029, china 2. Department of Biology, hebei normal university, Shijiazhuang 050016, china Correspondence should be addressed to Ma Ligeng (e-mail: ligeng.ma @yale.edu) Department of Horticultural Science Zhejiang University Department of Biology Hehei Normal University Shijiazhuang

Steroid hormones play essential roles in animal growth and development. Steroid signaling in animal system is focused on the direct gene regulation response mediated by its nuclear receptors. Recently, steroid hormones are also found in plants. Identification of BRI1 — a critical compo-nent of the plasma-membrane steroid receptor complex, and the related signal transduction pathway mediated by the membrane receptor have revealed an elementary picture of BR signaling from the cell surface perception to the activa-tion of BR-responsive nuclear genes.

关键词： plants Brassinosteroids signal transduction BRI1 cross talk.

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Aberrant histone H4 acetylation in dead somatic cell-cloned calves

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Progress in Natural Science:Materials International 2008年第7期18卷 813-818页

作者： Lei Zhang a,b,*, Shaohua Wang a, Qiang Li a, Xiangdong Ding c, Yunping Dai a, Ning Li a,* a State Key Laboratory for Agrobiotechnology, china Agricultural university, Beijing 100094, china b Beijing gene & Protein Biotechnology Co. LTD, Beijing 100094, china c College of Animal Science and technology, china Agricultural university, Beijing 100094, china State Key Laboratory for Agrobiotechnology China Agricultural University Beijing 100094 China Beijing gene & Protein Biotechnology Co. LTD Beijing 100094 China College of Animal Science and technology China Agricultural University Beijing 100094 China

In somatic cell-cloned animals, inefficient epigenetic reprogramming can result in an inappropriate gene expression and histone H4 acetylation is one of the key epigenetic modifications regulating gene expression. In this study, we investigated the levels of histone H4 acetylation of 11 development-related genes and expression levels of 19 genes in lungs of three normal control calves and nine aber- rant somatic cell-cloned calves. The results showed that nine studied genes had decreased acetylation levels in aberrant clones (p genes had no significant variations (p > 0.05). Whereas 13 genes had significantly decreased expression (p genes showed no significant differences between controls and clones (p > 0.05), and only one gene had higher expression level in clones (p hermore, FGFR, GHR, HGFR and IGF1 genes showed lowered levels of both histone H4 acetylation and expression in aberrant clones than in controls, and the level of histone H4 acetylation was even more lowered in aberrant clones than those in controls. It was suggested that the lower levels of histone H4 acetylation in aberrant clones caused by the previous memory of cell differentiation might not support enough chromatin reprogramming, thus affecting appropriate gene expressions, and growth and development of the cloned calves. To our knowledge, this is the first study on how histone H4 acetylation affects gene expression in organs of somatic cell-cloned calves.

关键词： Somatic cell-cloned calves Histone H4 acetylation gene expression Lungs

Identification and Characterization of a Rat Novel gene RSEP4 Expressed Specifically in Central Nervous System

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Acta Biochimica et Biophysica Sinica 2004年第7期36卷 501-507页

作者： Xi-Dao WANG1,2, Ling-Wei KONG1, Zhi-Qin XIE1, Yu-Qiu ZHANG3, Zhi-Xin LIN2, Zhi-Qi ZHAO3,Lei YU4, and Nai-he JING1* 1 Laboratory of Molecular Cell Biology, Laboratory of Stem Cell Biology, institute of Biochemistry and Cell Biology, Shanghai institutes for Biological Sciences, Chinese Academy of Sciences, Graduate School of the Chinese Academy of Sciences, Shanghai 200031, china School of Life Science and Biotechnology, 2 Shanghai Jiaotong university, Shanghai 200030, china institute of Neurobiology, Fudan university, Shanghai 200433, china 3 4 Department of Cell Biology, Neurobiology and Anatomy, College of Medicine, university of Cincinnati, Cincinnati, Ohio, USA Laboratory of Molecular Cell Biology and Stem Cell Biology Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Graduate School of the Chinese Academy of Sciences Shanghai China

The low-abundantly expressed genes composed the majorities of the mRNAs expressed in the central nervous system (CNS), and were thought to be important for the normal brain functions. Through differential screening a low-abundance cDNA sublibrary with mRNA from neuropathic pain of chronic constriction injury (CCI) model, we have identified a novel rat gene, rat spinal-cord expression protein 4 gene (RSEP4). The total length of RSEP4 cDNA is 2006 bp, with a 501 nucleotide open reading frame (ORF) that encodes a 167 amino acid polypeptide. Northern blot revealed that RSEP4 was expressed specifically in the CNS. In situ hybridization showed that the mRNA of RSEP4 was strongly expressed in the CA1, CA2, CA3 and DG regions of hippocampus, the Purkinje cells of cerebellum, and the small sensory neurons of dorsal horn and large motor neurons of ventral horn of spinal cord. Over-expression of RSEP4-EGFP fusion protein in the human embryonic kidney 293T cells showed that RSEP4 protein was mainly localized in the cell cytoplasm. These results suggest that RSEP4 may play some roles in the CNS.

关键词： central nervous system (CNS) rat Northern blot in situ hybridization

Trichostatin A Extends the Lifespan of Drosophila melanogaster by Elevating hsp22 Expression

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Acta Biochimica et Biophysica Sinica 2004年第9期36卷 618-622页

作者： Dan TAO#, Jun LU#, Hui SUN, Yan-Mei ZHAO, Zhi-Gen YUAN, Xiao-Xue LI, and Bai-Qu HUANG* institute of genetics and Cytology, Northeast normal university, Changchun 130024, china institute of genetics and Cytology Northeast Normal UniversityChangchun 130024China

The level of acetylation of histones in nucleosomes is related to the longevity of yeast and animals. However, the mechanisms by which acetylation and deacetylation affect longevity remain unclear. In present study, we investigated the influence of histone acetylation modification on the expression of hsp22 gene and the lifespan in Drosophila melanogaster using histone deacetylase (HDAC) inhibitor Trichostatin A (TSA). The results showed that TSA could extend the lifespan of Drosophila melanogaster. Furthermore, TSA significantly promoted the hsp22 gene transcription, and affected the chromatin morphology at the locus of hsp22 gene along the polytene chromosome. Present data implicate that TSA may affect the lifespan of Drosophila through changing the level of histone acetylation and influencing the expression of hsp22 gene that is related to aging.

关键词： histone acetylation histone deacetylase inhibitor Drosophila melanogaster hsp22 lifespan

A rice exocyst subunit gene SEC3A is required for anther dehiscence

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The Crop Journal 2024年第3期12卷 938-943页

作者： Ranran Tu Hong Wang Zhihao Sun Qinwen Zou Jiajun Wu Duo Wu Nan Wang Guanghua he Rice Research institute Key Laboratory of Application and Safety Control of Genetically Modified CropsAcademy of Agricultural SciencesSouthwest UniversityChongqing 400715China

Anther dehiscence controls optimal interaction between pollen and stigma,thereby determining the successful sexual *** regulators or mechanisms of this process remain ***,two CRISPR/Cas9 mutants of a rice exocyst subunit gene SEC3A,sec3a-1 and sec3a-2,showed anther indehiscence at anthesis and male sterility at *** viability and germination in the mutants were partly defective,whereas their female gametes undergone a normal *** or self-pollinated seeds could be produced by artificial pollination,suggesting potential use of a weak sec3a mutant as a female line during hybrid ***3A is widely expressed in various tissues,including anther *** results showed an excessive IAA accumulation and no endothecium lignification in sec3a-1/2 *** findings suggest that SEC3A appears to regulate anther dehiscence by modulating auxin signaling,providing insights into regulation of anther dehiscence and function of exocyst in plants.

关键词： Rice Male sterility Anther dehiscence Exocyst SEC3A

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