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2007中国科协年会

作者： Wang Binbin~1 Liu Deli~(1,2*) Zheng Yongliang~1 Zhang Qingye~2 Deng Lingfu~1 Wan Jian~2 1.College of Life Science,Central china normal university,Wuhan 430079,china 2.Key Laboratory of Pesticide & Chemical Biology(Huazhong university),Ministry of Education.

Organophosphate pesticides hydrolase gene(opd)was cloned from a novel methylparathion degradation bacterium Pseudomonas stutzeri *** phylogenetic tree of organophosphate pesticides hydrolase was constructed and the OPD protein structure was *** showed that the OPD protein from the strain HS-D36 was 99.0% similar to MPD protein(from pseudomonas ***-3)and it belonged to metallo-β-lactamases *** the predicted protein structure,the mature OPD comprise two independent subunits,each subunit is composed of an active metal center(Zn2+ and Cd2+).Homology modeling of OPD was constructed based on the crystal structure of MPD(PDBID:1P9E).There are three residues in OPD protein different from MPD.

关键词： Organophosphate pesticides hydrolase Sequence alignment OPD protein structure Active centre

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Molecular Cloning of a HMG-CoA Reductase gene from Euphorbia Pekinensis Rupr

Molecular Cloning of a HMG-CoA Reductase Gene from Euphorbia...

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江苏省遗传学会植物分子育种学术研讨会

作者： CAO Xiaoying1 JIANG Jihong1 DAI Chuanchao2 (1 Key Laboratory for Biotechnology on Medicinal Plants of Jiangsu Province , Xuzhou normal university, Xuzhou221116, china,2 College of Life Science, Nanjing normal university)

The 3-hydroxy-3-methylglutaryl-CoA reductase （HMGR） catalyzes the conversion of HMG-CoA to mevalonate, which is the first committed step in the pathway for isoprenoid biosynthesis in plants. A full-length cDNA encoding HMGR （designated as EpHMGR, GenBank Accession No. EF062569） was isolated from Euphorbia Pekinensis Rupr by rapid amplification of cDNA ends （RACE）. The full-length cDNA of EpHMGR comprises 2200 base pairs （bp） with a 1752-bp open reading frame （ORF） encoding a 583 amino-acid polypeptide revealed by bioinformatic analysis. Molecular modeling showed that EpHMGR is a new HMGR with a spatial structure similar to other plant HMGRs. The deduced protein has an isoelectric point （pI） of 6.51 and a calculated molecular weight of about 63 kDa. Sequence comparison analysis showed that EuHMGR had highest homology to HMGR from other plants. As expected, phylogenetic tree analysis indicated that EpHMGR belongs to plant HMGR group. Tissue expression pattern analysis showed that EpHMGR is strongly expressed in the roots and stems whereas is poorly expressed in the leaves, which implied that EpHMGR may be a constitutively expressing gene.

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gene expression profile induced by oral administration of baicalin and gardenin after focal brain ischemia in rats

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Acta Pharmacologica Sinica 2005年第3期26卷 307-314页

作者： Zhan-jun ZHANG~2 Zhong WANG~3 Xiao-yan ZHANG~4 Kang YING~5 Jian-xun LIU~3 Yong-yan WANG~62 The Clinical School of Beijing university of Traditional Chinese Medicine,Beijing 100029,china 3 Xi-yuan Hospital of the Academy o] Traditional Chinese Medicine,Beijing 100089,china 4 Beijing normal university,Beijing 100036,china 5 BioStar genechip Incorporated,Shanghai 200012,china 6 The Academy of Traditional Chinese Medicine,Beijing 100059,china TheClinicalSchoolofBeijinguniversityofTraditionalChineseMedicine Beijing100029China

Aim:To investigate differential gene expression and the pharmacological mecha-nism of baicalin and gardenin in focal cerebral ischemia in rats with high-densitycDNA ***:Rat left middle cerebral arteries were occluded andtreated with either baicalin or *** pharmacological effects were inves-tigated using the difference in infarction areas before and after treatment,whichwere determined by 2,3,5-triphenyltetrazolium chloride (TTC) *** ex-pression was demonstrated using a"Biostar40S"gene *** transcription-polymerase chain reaction (RT-PCR) was used to verify theresult of the selected ***:Both baicalin and gardenin reduced theinfarction areas in focal cerebral ischemia rats (Phe differential geneswere 211,177,and 70 (upregulated or downregulated) in the model group,baicalin,and gardenin treatment groups compared with the sham-operated group,*** expression of RpL19 and Csnk2 underwent an approximately1.9 and 2.1-fold increase,respectively,verified by semiquantitative RT-PCR,whichwas the same trend as the cDNA ***:Differential gene ex-pression with respect to the pharmacological effects of baicalin and gardenin onfocal cerebral ischemia by cDNA microarray revealed a number of clues with re-spect to the therapeutic mechanisms of Chinese traditional *** additionthe present study provided theoretical and experimental evidence that will aidfuture studies examining cerebral ischemia.

关键词： microarray gene expression baicalin gardenin brain ischemia

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Origin and evolution of vertebrate ABCA genes:a story from amphioxus

Origin and evolution of vertebrate ABCA genes:a story from a...

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遗传学进步与人口健康高峰论坛

作者： Guang Li~1 Qiu-Jin Zhang~(1,2) Zhi-Liang Ji~1 Yi-Quan Wang~1 (1.Key Laboratory of the Ministry of Education for Cell Biology and Tumor Cell Engineering,School of Life Sciences,Xiamen university,Xiamen,361005,china 2 College of Life Sciences,Fujian normal university,Fuzhou,350108,china)

Previous studies showed that the vertebrate ABCA subfamily,one subgroup of ATP-binding-cassette superfamily,had evolved rapidly in terms of gene duplication and *** further uncover the evolutionary history of the ABCA subfamily,we characterized ABCA members of two amphioxus species (Branchiostoma floridae and ***),the closest living invertebrate relatives to *** analysis indicated that these two species have the same set of ABCA genes (both containing six members).Five of these genes have clear orthologs in vertebrate,including one cephalochordate-specific duplication and one

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Knock-in of exogenous sequences based on CRISPR/Cas9 targeting autosomal genes and sex chromosomes in the diamondback moth,Plutella xylostella

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Journal of Integrative Agriculture 2024年第9期23卷 3089-3103页

作者： Shanyu Li Guifang Lin Haoqi Wen Haiyan Lu Anyuan Yin Chanqin Zheng Feifei Li Qingxuan Qiao Lu Jiao Ling Lin Yi Yan Xiujuan Xiang Huang Liao Huiting Feng Yussuf Mohamed Salum Minsheng You Wei Chen Weiyi he State Key Laboratory for Ecological Pest Control of Fujian and Taiwan Crops/Ministerial and Provincial Joint Innovation Centre for Safety Production of Cross-Strait Crops/International Joint Research Laboratory of Ecological Pest Control Ministry of EducationInstitute of Applied EcologyFujian Agriculture and Forestry UniversityFuzhou 350002China Ganzhou Key Laboratory of Greenhouse Vegetable Gannan Normal UniversityGanzhou 341000China

genetic pest control strategies based on precise sex separation and only releasing sterile males can be accomplished by site-specific genome *** the current study,we showed that the mutation of single-allele Pxfl(2)d can significantly impair the normal mating behavior and testis development in male adults of the notorious cruciferous insect pest Plutella xylostella,in addition to its known functions in the ovarian development in female adults and egg *** CRISPR/Cas9-based knock-in experiments revealed that site-specific integration of an exogenous green fluorescent protein(GFP)gene into autosomal Pxfl(2)d for labelling mutants could be ***,this gene is not a suitable target for GFP insertion to establish a genetically stable knock-in strain because of the severe decline in reproductive *** further screened for the W-chromosome-linked and Z-chromosome-linked regions to test the knock-in efficiency mediated by CRISPR/*** results verified that both types of chromosomes can be targeted for the site-specific insertion of exogenous *** ultimately obtained a homozygous knock-in strain with the integration of both Cas9 and cyan fluorescent protein(CFP)expression cassettes on a Z-linked region in ***,which can also be used for early sex *** injecting the sgRNA targeting Pxfl(2)d alone into the eggs laid by female adults of the Z-Cas9-CFP strain,the gene editing efficiency reached 29.73%,confirming the success of expressing a functional Cas9 *** together,we demonstrated the feasibility of the knock-in of an exogenous gene to different genomic regions in ***,while the establishment of a heritable strain required the positioning of appropriate *** study provides an important working basis and technical support for further developing genetic strategies for insect pest control.

关键词： cruciferous specialist fl(2)d CRISPR/Cas9 knock-in sex chromosome

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m^(6)A modification of lncRNA in middle ear cholesteatoma

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中南大学学报（医学版） 2024年第5期49卷 667-678页

作者： he Jun XIE Shumin JIN Li FU Jinfeng YUAN Qiulin LIU Wei Department of Otolaryngology-head and Neck Surgery Second Xiangya HospitalCentral South UniversityChangsha 410011 Clinical Medical Research Center for Otology in Hunan Province Changsha 410011 Department of Otolaryngology-head and Neck Surgery Xiangya HospitalCentral South UniversityChangsha 410008 Otolaryngology Major Disease Research Key Laboratory of Hunan Province Changsha 410008 Clinical Research Center for Pharyngolaryngeal Diseases and Voice Disorders in Hunan Province Changsha 410008 National Clinical Research Center for Geriatric Disorders Xiangya HospitalChangsha 410008China

Objective:Middle ear cholesteatoma is a non-tumorous condition that typically leads to hearing loss,bone destruction,and other severe *** surgery being the primary treatment,the recurrence rate remains ***,exploring the molecular mechanisms underlying cholesteatoma is crucial for discovering new therapeutic *** study aims to explore the involvement of N6-methyladenosine(m^(6)A)methylation in long non-coding RNAs(lncRNAs)in the biological functions and related pathways of middle ear ***:The m^(6)A modification patterns of lncRNA in middle ear cholesteatoma tissues(n=5)and normal post-auricular skin tissues(n=5)were analyzed using an lncRNA m^(6)A transcriptome *** Ontology(GO)and Kyoto Encyclopedia of genes and Genomes(KEGG)pathway analyses were conducted to identify potential biological functions and signaling pathways involved in the pathogenesis of middle ear *** RNA immunoprecipitation(MeRIP)-PCR was used to validate the m^(6)A modifications in cholesteatoma and normal skin ***:Compared with normal skin tissues,1525 lncRNAs were differentially methylated in middle ear cholesteatoma tissues,with 1048 showing hypermethylation and 477 showing hypomethylation[fold change(FC)≥3 orhe hedgehog signaling pathway,viral protein interaction with cytokines and cytokine receptors,mitogen-activated protein kinase(MAPK)signaling pathway,cytokine-cytokine receptor interaction,and adrenergic signaling in ***

关键词： long non-coding RNA m6A modifications middle ear cholesteatoma

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gene expression profiling of the whitefly(Bemisia tabaci)feeding on healthy and Tomato yellow leaf curl china virus infected tobacco

Gene expression profiling of the whitefly(Bemisia tabaci)fee...

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第三届全国生物入侵大会

作者： Jun-min Li~1,Yong-ming Ruan~2,Fang-fang Li~1,Shu-Sheng Liu~1 and Xiao-wei Wang~1 1.institute of Insect Sciences,Zhejiang university,Hangzhou,310029,china 2.College of Chemistry and Life Sciences,Zhejiang normal university,Jinhua,321004 china

Begomoviruses are exclusively transmitted by the whitefly(Bemisia tabaci) in a circulative, non-propagative *** influences of begomoviruses on whitefly vector are complex with both direct and indirect ***,the molecular mechanisms of the interactions underlying these effects are poorly understood and the transcriptional profiles of whitefly on healthy and virus-infected plant have not yet been *** suppression subtractive hybridization(SSH),we investigated the differentially expressed genes in a *** cryptic species(Middle East- Asia Minor 1) feeding on healthy and Tomato yellow leaf curl china virus(TYLCCNV) infected tobacco *** the forward cDNA library,124 differentially expressed Expression Sequence Tags(ESTs) were obtained which represent up-regulated genes in the whiteflies feeding on the infected *** the reverse library,112 ESTs were isolated which represent down-regulated *** the up-regulated genes, we identified several genes that are probably involved in direct interaction between whitefly and TYLCCNV,including a 26/29-kDa proteinase that appears to participate in the elimination of foreign proteins,heparan sulfate proteoglycan which mediates the entry of several viruses into host cells and two genes of Rickettsia-a secondary endosymbiotic bacterium of *** addition,we identified a number of genes involved in metabolism,transcription and translation which might be the result of indirect effects of TYLCCNV on the whitefly via host ***,our results suggest that TYLCCNV-infected tobacco changes the gene expression profiles of whitefly via both direct and indirect *** study revealed,for the first time,a number of genes involved in whitefly-TYLCCNV-tobacco interactions and provided useful information for future study on this complex system.

关键词： Begomovirus Bemisia tabaci Suppression subtractive hybridization

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Association of Polymorphisms in the Pit-1 Intron 5 with Growth Traits in Chinese Cattle

Association of Polymorphisms in the Pit-1 Intron 5 with Grow...

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第十次全国畜禽遗传标记研讨会

作者： YANG Dong-ying, CheN Hong, LAN Xian-yong, ZHANG Zheng-feng, TANG Li-gang, ZHANG Liang-zhi, WANG Xin-zhuang, WANG Yi-min, NIU Hu (College of Animal Science and technology, Northwest A&F university, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Yangling, Shaanxi 712100, china institute of Cellular and Molecular Biology, Xuzhou normal university, Xuzhou, Jiangsu 221116, china The Research Center of Beef Cattle Engineering and technology in henan, Zhengzhou, henan 450003, china)

The Pit-1 gene was studied as a candidate for genetic markers of growth traits in Chinese cattle in this study. The single-strand conformation polymorphism method was used to detecte polymorphism in the Pit-1 gene including intron 5. One single nucleotide polymorphism was found in intron 5 of Pit-1. The frequencies of alleles A/B in Nanyang cattle(NY), Qinchuan cattle(QC). Jiaxian Red cattle(JXR), Xizhen cattle(XZ), Luxi cattle(LX) and Holstein cow(H) populations were 0.444/0.556 , 0.477/0.523, 0.538/0.462, 0.421/0.579, 0.523/0.477, 0.475/0.525 respectively. Associations of the polymorphisms with growth traits in Nanyang cattle were analyzed using a general linear model procedure. The following parameters were greater in individuals with genotype AA than with genotype AB: body weight, average daily gain, body height, chest girth at 6, 12, 18 and 24 months(P allele A in other age groups. Therefore, genotype BB maybe a dominant genotype and allele B may be a dominant allele. These results imply that allele B of Pit-1 gene likely positively affects growth traits.

关键词： Beef cattle Growth traits Pit intron 5 Polymorphism

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Interaction of novel estrogen receptor alpha 36 and Caveolin-1 affects the metastasis of breast cancer cells

Interaction of novel estrogen receptor alpha 36 and Caveolin...

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中国生理学会第23届全国会员代表大会暨生理学学术大会

作者： Qian CheN~1,Yang WANG~1,Wei ZOU~(1,2,*) 1 College of Life Science,Liaoning normal university,Dalian 116029,china 2 The Key Laboratory of Biotechnology and Molecular Pharmacy in Liaoning Province,Dalian 116029,china

Over the last few decades there have been outstanding advances in breast cancer management leading to earlier detection of disease and the development of effective treatments,thus resulting in significant declines in breast cancer deaths and improved outcomes for women living with the *** cancer is a multi-faced disease comprised of distinct biological subtypes with different prognostic and therapeutic implications molecular ***-1 is the major protein component of caveolae,specialized lipid rafts that are recognized in electron micrographs as 50-100 nm invaginations of plasma ***,we established a Caveolin-1 haploinsufficiency cell line,we found that Caveolin-1 down-regulation could activate the express of ERα,leading to partial transformation of human breast epithelial *** newly established cell model,MCFIOA,expressed a low level of Caveolin-1 and a high level of ERα*** result showed that Caveolin-1 down-regulation strengthened the interaction of ERαand ***,Cav-1 gene silencing promoted cell adhesion,invasion and metastasis,while the inhibition of ERαsignal molecules attenuated the activity and expression of MMP-2, MMP-9 and other metastasis related proteins,which dependent on ERα36 was *** investigation revealed ERα36 mediated the cross-talk between PI3K/AKT and MEK/ERK pathways to promote cell growth. MCFIOA,MCF10A-ST1,MCF10A as well as wounded healing group,and simultaneously added E2 group were cultured in monolayer,and Western blot assay was *** results showed that Caveolin-1 down-regulation strengthened the expression of MMP-2,which was further increased in wounded healing group,but E-cadherin was *** MCF10A and MCF10A were treated with estrogen,estrogen recepter inhibitor(ICI182,780) and association treated were *** found that Caveolin-1 significantly increased in ICI182,780-treated ***,Caveolin-1 maybe works as a negative regulator of the sur

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SNPs of GH gene Characterized by PCR-SSCP Analysis and Search for Their Associations with Milk Yield in Goat

SNPs of GH Gene Characterized by PCR-SSCP Analysis and Searc...

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第十次全国畜禽遗传标记研讨会

作者： LAN Xian-Yong , CheN Hong, PAN Chuan-Ying , LEI Chu-Zhao (College of Animal Science and technology, Northwest A& F university, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Yangling, Shaanxi 712100, P. R. china institute of Cellular and Molecular Biology, Xuzhou normal university ,Xuzhou 221116, P. R. china)

Single nucleotide polymorphisms (SNPs) characterized by PCR-SSCP analysis in exon V of growth hormone (GH) gene for 180 goats from five goat breeds were investigated and their associations with milk yield of Xinong Saanen dairy goats were searched. The results showed that four SSCP patterns were observed in goat. In reference to goat GH gene sequence (GenBank No.D00476) as a control, SSCP pattern sequences characterized 14 SNPs, among which 4 codon mutations changed no amino acid variations. The establishment of relationships between SSCP patterns and SNPs and milk performance from Xinong Saanen dairy goats was attempted by a general mixed linear model. Significant statistical relationship between 3 SSCP patterns and milk yields was observed (Phence, we drew a preliminary conclusion that 4 SNPs characterized by PCR-SSCP patterns for exon V of GH gene exerted significant effects on milk performance, could be considered as molecular marker in MAS.

关键词： PCR-SSCP Single nucleotide polymorphisms (SNPs) GH gene Milk yield Association.

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