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SCAR Markers Assisted Selection for a Bentazon Susceptible Lethality gene (ben) in Rice

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Rice Science 2003年第Z1期 6-10页

作者： XIANG Tai-he~(1,2), YANG Jian-bo~2, YANG Qian-jin~3, ZHU Qi-sheng~3 , LI Li~2, HUANG Da-nian~4 (~1School of Life Sciences, Hangzhou normal College, Hangzhou 310036, china ~2Key Laboratory of Rice genetics Breeding, Ministry of Agriculture, P. R. china Rice Research institute, Anhui Academy of Agricultural Sciences, hefei 230031 , china ~3Natural Nourishing Food Engineering institute, Anhui Academy of Agricultural Sciences, hefei 230031 , china ~ ~ china National Rice Research institute, Hangzhou 310006, china)

In progenies resulting from crosses involving rice cultivar Norin 8m susceptible to bentazon as the donor of ben gene, SCARs tightly linked to ben were utilized for selection of ben. The homozygous and heterozygous genotypes with ben could be identified with the SCARs. The molecular markers offer a powerful tool for indirect selection of ben and can accelerate the introgression of ben into current rice cultivars.

关键词： rice bentazon susceptible lethality gene molecular marker assisted selection breeding

Modulation of inflammation-related genes of polysaccharides fractionated from mycelia of medicinal basidiomycete Antrodia camphorata

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Acta Pharmacologica Sinica 2007年第2期28卷 258-267页

作者： Yen-ying WU~3 Chin-chu CheN~4 Charng-chemg CHYAU~5 Sin-yi CHUNG~3 Yi-wen LIU~(2,3)3 Graduate institute of Biopharmaceutics,College of Life Sciences,National Chiayi university,Chiayi 600,Taiwan china4 institute of Biotechnology,National Tsing Hua university,Hsinchu 300,Taiwan,china 5 Department of Biotechnology Hung Kuang university,Taichung 433,Taiwan,china GraduateinstituteofBiopharmaceutics CollegeofLifeSciencesNationalChiayiUniversityChiayi600Taiwan china instituteofBiotechnology NationalTsingHuaUniversityHsinchu300TaiwanChina

Aim:To investigate the effect of water soluble-ethanol precipitation fraction(AC-1)and alkaline extraction-isoelectric precipitation fraction(AC-2)from Antrodiacamphorata(Polyporaceae,Aphyllophorales)on lipopolysaccharide(LPS)-in-duced gene activation in mouse ***:The AC-1 and AC-2fractions were prepared,and their effects on LPS-induced gene expression weremonitored by Western blotting and ***:Our results indicatedthatAC-2,but not AC-1 dose-dependently(50-200 mg/L)inhibited LPS-induced nitricoxide production as well as the protein and the mRNA expression of the induciblenitric oxide synthase(iNOS)*** AC-1 nor AC-2 inhibited LPS-inducedcyclooxygenase-2 gene *** the cytokine array assay,it showedthat AC-2 also had the ability to inhibit LPS-induced the protein expression ofinterleukin(IL)-6,1L-10,the monocyte chemoattractant protein(MCP)-5,and regu-lated upon activation,normal T-cell expressed,and presumably secreted(RANTES).Like iNOS,AC-2 inhibiting LPS-induced IL-6 and IL-10 secretion resulted frominhibiting their mRNA ***:It was suggested that alkalineextraction-isoelectric precipitated the polysaccharide fraction of A camphorataand had the ability to inhibit LPS-induced iNOS,IL-6,IL-10,MCP-5,and RANTESexpression in mouse macrophages.

关键词： Antrodia camphorata cytokines inducible nitric oxide synthase cyclooxygenase-2 macrophages

Research on the Expression of HOXA10 in Placental Villi during the Onset of Labor

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Journal of Reproduction and Contraception 2012年第2期23卷 67-74页

作者： Hong he De-min PU Rong LIU Qiong-hua CheN Dong-rui DENG Tian LI Department of Obstetrics and Gynecology Tongji Hospital Tongji Medical School Huazhong University of Science&TechnologyWuhan 430030China Department of Obstetrics and Gynecology Xiamen First Hospital Fujian Medical UniversityXiamen 350005China

Objective To investigate the difference of the expression of HOXAIO gene in placental villi before and after the onset of labor. Methods Immunohistochemistry was used to detect the location of HOXAIO in placental villi. The expression of HOXAIO mRNA and protein were measured by RT-PCR and Western blotting. Results lmmunohistochemistry showed that HOXAIO was only weakly expressed in placental villi of the natural labor group. However, it was strongly expressed in placental Villi of the surgical labor group. RT-PCR and Western blotting showed that the expression of HOXAIO mRNA and protein were significantly lower in the natural labor group than in the surgical labor group （P〈0.05）. Conclusion The lower expression of HOXAIO during the onset of labor may be one of the major factors in initiating the normal labor. And to some extent, the lower expression of HOXAIO gene in the natural labor group may be related to ＂functional progesterone withdrawal＂.

关键词： HOXAIO placental villi progesterone labor onset

Association and linkage analysis of COLIA1 and AHSG gene polymorphisms with femoral neck bone geometric parameters in both Caucasian and Chinese nuclear families

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Acta Pharmacologica Sinica 2007年第3期 375-381页

作者： Hui JIANG~(2,3,5) Shu-feng LEI~(2,5) Su-mei XIAO~2 Yuan CheN~(2,3) Xiao SUN~2 Fang YANG~(2,3) Li-ming LI~2 Shan WU~2 Hong-wen DENG~(2,4,6)2 Laboratory of Molecular and Statistical genetics and the Key Laboratory of Protein Chemistry and Developmental Biology of the Ministry of Education,College of Life Sciences,Hu-nan normal university,Changsha 410081,china 3 Osteoporosis Research Center,Creighton university,Omaha 68131,USA 4 Department of Orthopedic Surgery,School of Medicine,university of Missouri-Kansas City,Kansas City 64108,USA

Aim:To simultaneously investigate the contribution of the alpha 1 chain of col-lagen type 1(COLlAl)and alpha2-HS-glycoprotein(AHSG)genes to the varia-tion of bone geometric parameters in both Caucasians and ***:Sixhundred and five Caucasian individuals from 157 nuclear families and 1228 Chi-nese subjects from 400 nuclear families were genotyped at the AHSG-Sacl,COLIA1-PCOL2 and Spl polymorphisms using polymerase chain reaction(PCR)-restric-tion fragment length polymorphism(RFLP).5 FN bone geometric parameters werecalculated based on bone mineral density and bone area of femoral neck(FN)measured by dual energy X-ray *** stratification,totalfamily association,within-family association,and linkage tests were performed bythe quantitative transmission disequilibrium test ***:The t-testshowed the significant differences of all bone geometric phenotypes(except ED)between Caucasians and Chinese in the offspring using both unadjusted andadjusted(by age,height,weight,and gender)*** Caucasians,we foundsignificant within-family association results between the COLlAl-Spl polymor-phism(rs1800012)and cross sectional area(CSA),cortical thickness(CT),endocortical diameter(ED),buckling ratio(BR)(P=0.018,0.002,0.023,and 0.001,respectively);the COLlAl-Spl polymorphism also detected significant linkagewith BR(P=0.039).In the population of china,the within-family associationsbetween the COLlAl-PCOL2 polymorphism(rs1107946)and CT,BR were signifi-cant(P=0.012 and 0.008,respectively).Furthermore,evidence of linkage wereobserved between the AHSG-Sacl polymorphism(rs4918)and CT,BR(P=0.042and 0.014,respectively)in Caucasians,but not in ***:Ourresults suggest that the COLlAl gene may have significantly association withbone geometry in both Caucasians and Chinese,and the AHSG gene may belinked to bone geometry in Caucasians,but not in *** study representsour first efforts on investigating the importance of the COLlAl and A

关键词： COLIA1 AHSG bone geometry transmission disequilibrium test

Screening of GJB2 mutations in Chinese population

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Journal of Otology 2007年第1期2卷 18-22页

作者： HAN Ming-kun1, HAN Dong-yi1, GUO Yu-fen2, LI Qing-zhong3, ZHAO Ya-li1, 4, RAO Shao-qi1, 5, 6, YUAN Hu1, ZONG Liang1, GUAN Jing2, XU Bai-cheng2, WANG Da-yong1, LAN lan1, WANG Qiu-ju1, 7 1 Department of Otorhinolaryngology/ head and Neck Surgery, and institute of Otolaryngology, china People Liberation Army general Hospital, Beijing, china 2 Department of Otorhinolaryngology/ head and Neck Surgery, the Second Affiliated Hospital of LanZhou university, Lanzhou, china 3 Deparment of Otlolaryngology, Eye and ENT Hospital of Fudan university, Shanghai, china 4 Department of Biochemistry and Molecular Biology, institute of Basic Medical Sciences, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, china 5 Departments of Cardiovascular Medicine and Molecular Cardiology, the Cleveland Clinic Foundation, Cleveland, Ohio, USA 6 Department of Bioinformatics, Harbin Medical university, Harbin, china 7 china National Human Genome Center, Beijing, china Department of Otorhinolaryngology/ head and Neck Surgery and Institute of Otolaryngology China People Liberation Army General Hospital Beijing China Department of Otorhinolaryngology/ head and Neck Surgery the Second Affiliated Hospital of LanZhou University Lanzhou China Deparment of Otlolaryngology Eye and ENT Hospital of Fudan University Shanghai China Department of Biochemistry and Molecular Biology Institute of Basic Medical Sciences Chinese Academy of Medical Sciences and Peking Union Medical College Beijing China Departments of Cardiovascular Medicine and Molecular Cardiology the Cleveland Clinic Foundation Cleveland Ohio USA Department of Bioinformatics Harbin Medical University Harbin China china National Human Genome Center Beijing China

The GJB2 gene(connexin 26) has been shown to be responsible for DFNB1 and DFNA3. We screened the GJB2 gene in 488 patients with prelingual deafness(Group 1), 124 with postlingual deafness(Group 2), and 117 normal hearing subjects(Group 3). We found that, in Group 1, 65 patients(13.32%) were homozygotes or compound heterozygotes and 51 patients (10.45%) carried a single pathogenic mutation. The 235delC mutation was the most frequent mutation, accounting for 73.22% of the known pathogenic alleles in Group 1. No homozygotes or compound heterozygotes were detected in Group 2 or Group 3. Some postlingual deaf patients (2.42%) and normal hearing subjects(4.27%) were 235delC carriers. Our preliminary data indicate that 235delC, the most frequent mutation identified in this study, is a major cause for prelingual deafness.

关键词： prelingual deafness postlingual deafness, GJB2 235delC Chinese

Demographic history of the Tibetan antelope Pantholops hodgsoni(chiru)

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Journal of Systematics and Evolution 2010年第6期48卷 490-496页

作者： 1,2,3Yu-Rong DU 1Song-Chang GUO 4Zhao-Feng WANG 1Hai-Xing CI 1Zhen-Yuan CAI 4Qian ZHANG 1Jian-Ping SU 4Jian-Quan LIU 1(Key Laboratory of Evolution and Adaptation of Plateau Biota,Northwest institute of Plateau Biology,Chinese Academy of Sciences,Xining 810001,china) 2(Graduate university of the Chinese Academy of Sciences,Beijing 100049,china) 3(School of Life and Geography Science,Qinghai normal university,Xining 810008,china) 4(Key Laboratory of Arid and Grassland Ecology,School of Life Science,Lanzhou university,Lanzhou 730000,china) (Key Laboratory of Evolution and Adaptation of Plateau Biota Northwest Institute of Plateau Biology Chinese Academy of Sciences Xining 810001 China) (Graduate university of the Chinese Academy of Sciences Beijing 100049 China) (School of Life and Geography Science Qinghai Normal University Xining 810008 China) (Key Laboratory of Arid and Grassland Ecology School of Life Science Lanzhou University Lanzhou 730000 China) *Correspondence: Jian-Ping SU Jian-Quan LIU *Correspondence: ?Authors for correspondence. J-P SU E-mail: sujp@*** Tel.: 86-971-6143748 J-Q LIU E-mail: liujq@*** Tel.: 86-931-8914255.[?]?These authors contributed equally to this work.

The Tibetan antelope(chiru,Pantholops hodgsoni),a heavily poached species and symbol of the QinghaiTibetan Plateau(QTP),is noted worldwide for its special calving *** species originated in the early Quaternary and it is interesting to know how the following climatic oscillations affected its demographic dynamics in the climate-sensitive *** this study,we analyzed the mitochondrial D-loop region from 312 individuals sampled in all of the six major *** found high rates of gene flow and little genetic differentiation between populations,suggesting that the calving migration may have homogenized the genetic pool of this *** mismatch distribution analyses and coalescent simulations suggested that this species experienced a demographic expansion approximately 600-200 Kyr following the retreat of the large glaciers developed in the QTP at 800-600 Kyr,rather than at the end of the last glacial age,as previously suggested,based on a limited sample *** addition,we found evidence of a chiru population decrease probably related to the human settings at the QTP during the middle Holocene.

关键词： chiru calving migration demographic expansion mitochondrial D-loop Quaternary.

Early Growth Response-1 Accelerates Liver Regeneration after Partial hepatectomy through GGPPS in Mice

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Early Growth Response-1 Accelerates Liver Regeneration after...

机器学习联合生物信息学鉴定骨关节炎细胞衰老关键基因及验证

“细胞活动生命活力”——中国细胞生物学学会全体会员代表大会暨第十二次学术大会

作者： Lai Shanshan~1,Shen Ning~2,Li Chaojun~2,Xue Bin~2 1.The Medical School of Nanjing university,Nanjing 210093, 2.china Jiangsu Key Laboratory for Molecular and Medical Biotechnology,Nanjing normal university,Nanjing 210097,china

Background & Aims:Liver regeneration comprises a series of complicated processes,in which several transcription factors play an important ***-1 is rapidly induced as part of the immediate-early gene response of the regenerating *** studies indicate that EGR-1-/- exhibits delayed hepatocellular mitotic progression after partial hepatectomy and increased liver injury after carbon tetrachloride(CC14) *** the mechanism of Egr-1 in regulates regeneration progress is still *** aim is to reveal an important signal pathway mediated by Egr-1 in this ***:70%partial hepatectomy(PH) were performed using mice tail vein injection of adenoviral vectors expressing GFP (Ad-GFP),Egr-l(Ad-Egr-l) or Dominant negative Egr-l(Ad-DNEgr-l) to increase or down-regulate hepatic Egr-1 levels,and mice lack of ggpps in hepatocyte(GGPPS-/-).Animals were sacrificed at intervals after the surgery,and the remnant liver was harvested and *** in vitro study,serum starvation recovery model was used to mimic liver regeneration process in normal human *** signal pathway was detected by western ***:Loss of Egr-1 function in mice liver caused a delayed liver regeneration process reflected by regeneration ratio and expression of cell-cycle related proteins, increased ALT and AST level and ***-expressed Egr-1 can promoted GGPPS transcription increased Ras prenylation and finally caused Erk1/2 activation in ***-1/GGPPS/ ERK pathway contributes to the expression of cyclins in serum or partial hepatectomy induced cell cycle of hepatocytes. GGPPS-/- mice also shows decreased PCNA,CyclinD and CyclinE ***:Egr-1 plays an important role in regulating the cell cycle of hepatocytes induced by partial ***-1 contributes to the expression of cyclins through activating ggpps transcription,which leads to the activation of Ras/Raf/MAPK ***-1 gives positive feedback to MAPK pathway,whi

关键词： early growth response 1 liver regeneration GGPPS

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中国组织工程研究 2024年第20期28卷 3196-3201页

作者：袁长深廖书宁李哲吴思萍陈乐伟刘晋邑李彦宏段戡广西中医药大学第一附属医院四肢骨伤科广西壮族自治区南宁市530023 广西中医药大学研究生院广西壮族自治区南宁市530000

背景:细胞衰老与骨关节炎的发生、发展密切相关,但具体作用靶点及调控机制尚不清楚。目的:综合生物信息学和机器学习方法挖掘细胞衰老介导骨关节炎的关键基因,并利用实验加以验证,探讨细胞衰老在骨关节炎中的作用。方法:从GEO数据库中... 详细信息

背景:细胞衰老与骨关节炎的发生、发展密切相关,但具体作用靶点及调控机制尚不清楚。目的:综合生物信息学和机器学习方法挖掘细胞衰老介导骨关节炎的关键基因,并利用实验加以验证,探讨细胞衰老在骨关节炎中的作用。方法:从GEO数据库中获得骨关节炎基因表达谱以及从CellAge数据库中获得细胞衰老相关基因,二者取交集并提取交集基因表达量进行差异分析,然后对差异基因进行GO和KEGG分析,接着通过PPI网络分析和机器学习筛选骨关节炎细胞衰老关键基因,并进行体外细胞实验验证,采用qPCR方法检测关键基因的表达。结果与结论:①获得31个骨关节炎细胞衰老差异基因,GO分析显示主要参与白细胞分化调节、单核细胞分化、T细胞分化调节等生物过程,在DNA转录因子结合、组蛋白去乙酰化酶结合、染色质DNA结合、趋化因子结合中发挥作用;②KEGG分析表明骨关节炎细胞衰老差异基因主要在JAK/STAT信号通路、PI3K/Akt信号通路、FoxO信号通路中被激活;③通过PPI网络拓扑学分析和机器学习方法筛选出骨关节炎细胞衰老关键基因MYC;④体外细胞实验结果表明,实验组骨关节炎软骨细胞中MYC mRNA表达明显低于对照组(正常软骨细胞),差异有显著性意义(P<0.05);⑤结果表明,MYC可作为骨关节炎细胞衰老的关键基因,可能通过介导免疫应答、炎症反应和转录调控等成为骨关节炎防治的新靶标。

关键词：骨关节炎细胞衰老关键基因细胞实验机器学习生物信息学

heat shock response and mammal adaptation to high elevation(hypoxia)

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Science china(Life Sciences) 2006年第5期49卷 500-512页

作者： WANG Xiaolin1,2,3,XU Cunshuan4,WANG Xiujie5,WANG Dongjie6,WANG Qingshang7 & ZHANG Baochen11.Northwest Plateau Biological Research institute,Chinese Academy of Sciences,Xining 810001,china 2.Graduate School of the Chinese Academy of Sciences,Beijing 100039,china 3.Affiliated Hospital of Medical College of Qinghai university,Xining 810001,china 4.Key Laboratory of Ministry of Science and technology of china in henan normal university,Xinxiang 453000,china 5.Key Laboratory of china Ministry of Education in Oncology Research institute of Western Hospital of Sichuan university,Chengdu 610041,china 6.Guangzhou Meizheng Pharmaceutical Industry & Key Laboratory of Ministry of health of china,Guangzhou 510075,china 7.Rubber Research institute,china Tropical Agricultural Academy of Sciences,Haikou 571737,china 1. Northwest Plateau Biological Research institute Chinese Academy of Sciences Xining 810001 China 2. Graduate School of the Chinese Academy of Sciences Beijing 100039 China 3. Affiliated Hospital of Medical College of Qinghai university Xining 810001 China 4. Key Laboratory of Ministry of Science and technology of china in henan normal university Xinxiang 453000 China 5. Key Laboratory of china Ministry of Education in Oncology Research institute of Western Hospital of Sichuan university Chengdu 610041 China 6. Guangzhou Meizheng Pharmaceutical Industry & Key Laboratory of Ministry of health of china Guangzhou 510075 China 7. Rubber Research institute China Tropical Agricultural Academy of Sciences Haikou 571737 China

The mammal's high elevation(hypoxia) adaptation was studied by using the immu-nological and the molecular biological methods to understand the significance of Hsp(hypoxia) ad-aptation in the organic high elevation,through the mammal heat shock response.(1) From high ele-vation to low elevation(natural hypoxia) :Western blot and conventional RT-PCR and real-time fluo-rescence quota PCR were *** difference of heat shock protein of 70(Hsp70) and natural expression of brain tissue of Hsp70 gene was determined in the cardiac muscle tissue among the different elevation mammals(yak) .(2) From low elevation to high elevation(hypoxia induction) :The mammals(domestic rabbits) from the low elevation were sent directly to the areas with different high elevations like 2300,3300 and 5000 m above sea level to be raised for a period of 3 weeks be-fore being slaughtered and the genetic inductive expression of the brain tissue of Hsp70 was deter-mined with *** result indicated that all of the mammals at different elevations possessed their heat shock response ***70 of the high elevation mammal rose abruptly under stress and might be induced to come into being by high elevation(hypoxia) .The speedy synthesis of Hsp70 in the process of heat shock response is suitable to maintain the cells' normal physiological functions under *** Hsp70 has its threshold *** altitude of 5000 m above sea level is the best condition for the heat shock response,and it starts to reduce when the altitude is over 6000 m above sea *** Hsp70 production quantity and the cell hypoxia bearing capacity have their direct ratio.

关键词： heat shock reaction, heat shock protein 70 (Hsp70), Western blot, RT-PCR, fluorescence quota PCR, high elevation hypoxia, mammal, stress.