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Purification and characterization of a novel cholesterol-lowering protein from the seeds of Senna obtusifolia

第六届海南省科技论坛“医药科技创新与药品质量安全”专题论坛、海南省药学会2012年学术年会

作者： li Hong-fu~(1,2,3),li Yong-hui~(1,2,3*),Zhang Jun-qing~(1,2,3**), Wang Yong~(1,2,3),Wei Na~(1,2,3),Jan Yin-feng~(1,2,3) 1 Department of pharmacy,hainan Medical College.haikou 571101,PR china 2.hainan Key Laboratory for R&D of Tropical Medical Herbs,haikou 571101,PR china 3 hainan provincial institute of south china medicine and li nationality medicine,haikou 571101,china

Objective To study the chemical constituents in the stems and leaves of Alpinia oxyphylla *** The chemical constituents were isolated by using various column chromatorgaphies and their structures were elucidated on basis of physicochemical constants and spectral data *** Nine compounds were obtained and determined to be p-hydroxycinnamic acid(1),3,4-dihydroxybenzamide(2),β-sitosterol(3),tectochrysin(4), chrysin(5),izalpinin(6),pinocembrin(7).pinostrobin(8) and myristic acid(9).Conclusion: Compounds 1,2,7 and 8 were obtained from this genus for the first time.

关键词： Alpinia oxyphylla Miq. chemical constituents pinocembrin pinostrobin

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Science china(life Sciences) 2008年第11期51卷 1020-1024页

作者： li ChuHua1, li Mei2, CHANG WenRui2 & GUO BaoJiang1 1 College of life Sciences, south china Normal University, Guangdong provincial Key Lab of Biotechnology of Plant Development, Guang-zhou 510631, china 2 institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, china 1. College of life Sciences South China Normal University Guangdong Provincial Key Lab of Biotechnology of Plant Development Guangzhou 510631 China 2. institute of Biophysics Chinese Academy of Sciences Beijing 100101 China

"Juemingzi", a source of traditional Chinese herbal medicine, has been demonstrated to play a role in decreasing serum cholesterol concentration. In this study, a novel protein, which has shown an in-hibitory effect on cholesterol biosynthesis, was isolated from Senna obtusifolia L. seed by gel filtration and ion exchange chromatography. The novel protein’s molecular mass was 19.7 kD and its pI was 4.80. Both SDS-PAGE and isoelectric-focusing (IEF) revealed a single Coomassie brilliant blue stained band, indicating that the novel protein was a single peptide. The N-terminal amino acid sequence of the pro-tein was IPYISASFPLNIEFLPSE, which had no similarity with any other protein sequences in the NCBI protein database. Circular dichroism (CD) signals indicated that S. obtusifolia seed protein contained 12.5% α-helix, 55.6% β-sheet, and 31.9% random coil.

关键词： Senna obtusifolia L. seed novel protein purification biochemical properties cholesterol biosynthesis

M^(6)A modification in cardiovascular disease:With a focus on programmed cell death

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Genes & Diseases 2024年第5期11卷 272-284页

作者： Wen li Yao liu Ruiyan Xu Yuan Zong Lu He Jun Hu Guohua li institute of Cardiovascular Disease Key Laboratory for Arteriosclerology of Hunan ProvinceHunan International Scientific and Technological Cooperation Base of Arteriosclerotic DiseaseDepartment of PathophysiologyMOE Key Lab of Rare Pediatric DiseasesHengyang Medical SchoolUniversity of South ChinaHengyangHunan 421001China Department of Cardiovascular medicine The Second Affiliated HospitalHengyang Medical SchoolUniversity of South ChinaHengyangHunan 421001China Department of Obstetrics and Gynecology The First Affiliated HospitalHengyang Medical SchoolUniversity of South ChinaHengyangHunan 421001China Department of Cardiovascular Surgery The Second Affiliated HospitalHengyang Medical SchoolUniversity of South ChinaHengyangHunan 421001China

N^(6)-methyladenosine(m^(6)A)methylation is one of the most predominant internal RNA modifications in eukaryotes and has become a hot spot in the field of epigenetics in recent *** diseases(CVDs)are a leading cause of death *** evidence demonstrates that RNA modifications,such as the m^(6)A modification,are associated with the development and progression of many diseases,including *** increasing body of studies has indicated that programmed cell death(PCD)plays a vital role in ***,the molecular mechanisms underlying m^(6)A modification and PCD in CVDs remain poorly ***,elaborating on the highly complex connections between the m^(6)A mechanisms and different PCD signaling pathways and clarifying the exact molecular mechanism of m^(6)A modification mediating PCD have significant meaning in developing new strategies for the prevention and therapy of *** is great potential for clinical application.

关键词： Apoptosis Autophagy Cardiovascular diseases Ferroptosis N^(6)-methyladenosine Programmed cell death Pyroptosis

DNA Methylation Variation Is Identified in Monozygotic Twins Discordant for Congenital Heart Diseases

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Congenital Heart Disease 2024年第2期19卷 247-256页

作者： Shuliang Xia Huikang Tao Shixin Su Xinxin Chen li Ma Jianru li Bei Gao Xumei liu Lei Pi Jinqing Feng Fengxiang li Jia li Zhiwei Zhang Guangdong provincial People’s Hospital Southern Medical UniversityGuangzhou510100China Department of Cardiovascular Surgery Guangzhou Women and Children’s Medical CenterGuangzhou Medical UniversityGuangdong Provincial Clinical Research Center for Child HealthGuangzhou510623China Department of Pediatric Cardiology Guangdong Cardiovascular InstituteGuangdong Provincial Key Laboratory of South China Structural Heart DiseaseGuangzhou510100China Clinical Physiology Laboratory Institute of PediatricsGuangzhou Women and Children’s Medical CenterGuangzhou Medical UniversityGuangdong Provincial Key Laboratory of Research in Structural Birth Defect DiseaseGuangzhou510623China Department of Echocardiogram Guangzhou Women and Children’s Medical CenterGuangzhou Medical UniversityGuangdong Provincial Clinical Research Center for Child HealthGuangzhou510623China Department of Pediatrics Washington University School of MedicineSt.LouisMO63130USA Clinical Biological Resource Bank of Guangzhou Women and Children’s Medical Center Guangzhou Medical UniversityGuangzhou510623China

Aims:Multiple genes and environmental factors are known to be involved in congenital heart disease(CHD),but epigenetic variation has received little ***(MZ)twins with CHD provide a unique model for exploring this *** order to investigate the potential role of Deoxyribonucleic Acid(DNA)methyla-tion in CHD pathogenesis,the present study examined DNA methylation variation in MZ twins discordant for CHD,especially ventricular septal defect(VSD).Methods and Results:Using genome-wide DNA methylation profiles,we identified 4004 differentially methylated regions(DMRs)in 18 MZ twin pairs discordant for CHD,and 2826 genes were *** Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)analysis revealed a list of CHD-associated *** further investigate the role of DNA methylation in VSD,data from 7 pairs of MZ twins with VSD were *** identified 1614 DMRs corresponding to 1443 genes associated with arrhythmogenic right ventricular cardiomyopathy,cyclic guanosine monopho-sphate-protein kinase G(cGMP-PKG)signaling pathway by KEGG analysis,and cell-cell adhesion,calcium ion transmembrane transport by GO analysis.A proportion of DMR-associated genes were involved in calcium signaling *** methylation changes of calcium signaling genes might be related to VSD ***:CHD is associated with differential DNA methylation in MZ *** may be etiologically linked to DNA methylation,and methylation of calcium signaling genes may be involved in the development of VSD.

关键词： Congenital heart disease monozygotic twins methylation modification epigenetics

Antimicrobial roles of phagocytosis in teleost fish:Phagocytic B cells vs professional phagocytes

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Aquaculture and Fisheries 2024年第2期9卷 105-114页

作者： liting Wu Lan li Along Gao Jianmin Ye Jun li Guangdong provincial Key Laboratory for Healthy and Safe Aquaculture Institute of Modern Aquaculture Science and EngineeringSchool of Life SciencesSouth China Normal UniversityGuangzhou510631China Guangdong Province Key Laboratory of Waterfowl Healthy Breeding Zhongkai University of Agriculture and EngineeringGuangzhou510225China School of Science and medicine Lake Superior State UniversitySault Ste.MarieMI49783USA

The defense system of teleost fish organized on innate and adaptive immunity protects them against a wide variety of pathogenic microorganisms in the aquatic *** is one of the most effective defense strategies against microbial challenge mainly performed by classical‘professional’phagocytes(including monocytes,macrophages and granulocytes).They contain,kill and process the internalized pathogens for antigen presentation by providing antigenic ligands to initiate activation and clonal expansion of T and B cells,which bridge the innate and adaptive *** discovery of phagocytic B cells in teleost fish has broken the paradigm that primary vertebrate B cells are lack of phagocytosis of particulates,as well as led to the investigation of phagocytic activity of mammalian B-1 B *** active phagocytic,microbicidal capabilities and antigen presentation in teleost phagocytic B cell have demonstrated to be similar as professional phagocytes,providing a potential impact on development of new vaccination strategies to prevent and control infectious *** this review,we aim to address current progress on the antimicrobial role of phagocytic B cells in teleost fish by comparing it with other professional phagocytes and mammalian B-1 B cells,and provide the application prospect of phagocytic B cells in developing vaccines as well as the prevention of fish diseases.

关键词： Teleost fish Phagocytic B cell Phagocytosis Antimicrobial role Vaccine design

Pannexins in the musculoskeletal system:new targets for development and disease progression

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Bone Research 2024年第2期12卷 255-269页

作者： Yan Luo Shengyuan Zheng Wenfeng Xiao Hang Zhang Yusheng li Department of Orthopedics Xiangya HospitalCentral South UniversityChangshaHunan 410008China National Clinical Research Center for Geriatric Disorders Xiangya HospitalCentral South UniversityChangshaHunan 410008China Department of Clinical medicine Xiangya Medicine SchoolCentral South UniversityChangshaHunan 410008China Shenzhen institute of Advanced Technology Chinese Academy of SciencesShenzhen 518055China

During cell differentiation,growth,and development,cells can respond to extracellular stimuli through communication ***(Panx)family and connexin(Cx)family are two important types of channel-forming *** family contains three members(Panx1-3)and is expressed widely in bone,cartilage and *** there is no sequence homology between Panx family and Cx family,they exhibit similar configurations and *** to Cxs,the key roles of Panxs in the maintenance of physiological functions of the musculoskeletal system and disease progression were gradually revealed ***,we seek to elucidate the structure of Panxs and their roles in regulating processes such as osteogenesis,chondrogenesis,and muscle *** also focus on the comparison between Cx and *** a new key target,Panxs expression imbalance and dysfunction in muscle and the therapeutic potentials of Panxs in joint diseases are also discussed.

关键词： skeletal diseases system

Real-world data on ALK rearrangement test in Chinese advanced non-small cell lung cancer(RATICAL):a nationwide multicenter retrospective study

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Cancer Communications 2024年第9期44卷 992-1004页

作者： lin li Wencai li ChunyanWu Yanfeng Xi Lei Guo Yuan Ji lili Jiang Ji li Jingping Yun Gang Chen Yuan li Yueping liu DianbinMu Yuchen Han Leina Sun Qingxin Xia Xiaodong Teng Nanying Che WeiWu Xueshan Qiu Chao liu Xiaochu Yan Daiqiang li Zhihong Zhang ZheWang Yujun li ZhengWang lingchuan Guo Xiu Nie Jingshu Geng Jianhua Zhou Jianming Ying Department of Pathology National Cancer Center/National Clinical Research Center for Cancer/Cancer HospitalChinese Academy of Medical Sciences and Peking UnionMedical CollegeBeijingP.R.China Department of Pathology the First Affiliated Hospital of Zhengzhou UniversityZhengzhouHenanP.R.China Department of Pathology Shanghai Pulmonary HospitalSchool of MedicineTongji UniversityShanghaiP.R.China Department of Pathology Cancer Hospital Affiliated to Shanxi Medical UniversityShanxi Province Cancer HospitalShanxi Hospital Affiliated to Cancer HospitalChinese Academy of Medical SciencesTaiyuanShanxiP.R.China Department of Pathology Zhongshan HospitalFudan UniversityShanghaiP.R.China Department of Pathology West China Hospital of Sichuan UniversityChengduSichuanP.R.China Department of Pathology Peking Union Medical College HospitalChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijingP.R.China Department of Pathology Sun Yat-sen University Cancer CenterState Key Laboratory of Oncology in South ChinaCollaborative Innovation Center for Cancer MedicineGuangzhouGuangdongP.R.China Department of Pathology Fujian Cancer HospitalFujian Medical University Cancer HospitalFuzhouFujianP.R.China Department of Pathology Department of OncologyFudan University Cancer CenterShanghaiMedical College of Fudan UniversityShanghaiP.R.China Department of Pathology The Fourth Hospital of Hebei Medical UniversityShijiazhuangHebeiP.R.China Department of Pathology Shandong Cancer HospitalJinanShandongP.R.China Department of Pathology Shanghai Chest HospitalShanghai Jiao Tong UniversityShanghaiP.R.China Department of Pathology Tianjin Medical University Cancer Institute and HospitalTianjinP.R.China Department of Pathology the Affiliated Cancer Hospital of Zhengzhou UniversityHenan Provincial Cancer HospitalZhengzhouHenanP.R.China Department of Pathology the First Affiliated HospitalCollege of MedicineZhejiang UniversityHangzhouZhejiangP.R.China Department of Pathology Bei

Background:Anaplastic lymphoma kinase(ALK)test in advanced non-small cell lung cancer(NSCLC)can help physicians provide target therapies for patients harboring ALK gene *** study aimed to investigate the real-world test patterns and positive rates of ALK gene rearrangements in advanced ***:In this real-world study(ChiCTR2000030266),patientswith advanced NSCLC who underwent an ALK rearrangement test in 30 medical centers in china between October 1,2018 and December 31,2019 were retrospectively *** training was conducted before the study was *** controls were performed at participating centers using immunohistochemistry(IHC)-*** positive ALK gene rearrangement rate and consistency rate were *** associated clinicopathological characteristics of ALK gene rearrangement were investigated as ***:The overall ALK gene rearrangement rate was 6.7%in 23,689 patients with advanced NSCLC and 8.2%in 17,436 patients with advanced lung *** quality control analysis of IHC-VENTANA-D5F3 revealed an intrahospital consistency rate of 98.2%(879/895)and an inter-hospital consistency rate of 99.2%(646/651).IHC-VENTANA-D5F3 was used in 53.6%,real-time polymerase chain reaction(RT-PCR)in 25.4%,next-generation sequencing(NGS)in 18.3%,and fluorescence in-situ hybridization(FISH)in 15.9%in the adenocarcinoma *** specimens tested with multiple methods,the consistency rates confirmed by IHC-VENTANA-D5F3 were 98.0%(822/839)for FISH,98.7%(1,222/1,238)forNGS,and 91.3%(146/160)for *** overall ALK gene rearrangement rateswere higher in females,patients of≤35 years old,never smokers,tumor cellularity of>50,and metastatic specimens used for testing in the total NSCLC population and adenocarcinoma subgroup(all Plights the real-world variability and challenges of ALK test in advanced NSCLC,demonstrating a predominant use of IHCVENTANA-D5F3 with high co

关键词： anaplastic lymphoma kinase diagnosis gene fusion non-small cell lung cancer

Effects of weaning on intestinal longitudinal muscle-myenteric plexus function in piglets

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Science china(life Sciences) 2024年第2期67卷 379-390页

作者： Jing li Fenfen liu Kaibin Mo Hengjia Ni Yulong Yin Key Laboratory of Agro-Ecological Processes in Subtropical Region Hunan Provincial Key Laboratory of Animal Nutritional Physiology and Metabolic ProcessHunan Research Center of Livestock and Poultry SciencesSouth Central Experimental Station of Animal Nutrition and Feed Science in the Ministry of AgricultureInstitute of Subtropical AgricultureChinese Academy of SciencesChangsha 410125China University of Chinese Academy of Sciences Beijing 100008China Guangdong Key Laboratory for Veterinary Drug Development and Safety Evaluation College of Veterinary MedicineSouth China Agricultural UniversityGuangzhou 510642China

Weaning piglets usually suffer from severe diarrhea(commonly known as postweaning diarrhea,PWD)along with intestinal motility *** peristalsis is mainly regulated by the longitudinal muscle-myenteric plexus(LM-MP).To understand the relationship between intestinal LM-MP function and the development of PWD,we compared the intestinal electrical activity,and the transcriptional profile of the LM-MP between 21-day-old piglets(just weaned,n=7)and 24-day-old piglets(suffered the most severe weaning stress,n=7).The results showed that 24-day-old piglets exhibited different degrees of diarrhea.A significant increase in the slow-wave frequency in the ileum and colon was observed in 24-day-old piglets,while c-kit expression in the intestinal LM-MPs was significantly decreased,indicating that PWD caused by elevated slow-wave frequency may be associated with loss of *** real-time quantitative PCR(RT-q PCR)and enzyme-linked immunosorbent assay(EliSA)showed that intestinal LM-MPs in 24-day-old piglets may undergo inflammation and oxidative *** increases in 8-hydroxy-2′-deoxyguanosine and decreases in thioredoxin suggest that weaning may lead to DNA damage in the LM-MP of 24-day-old *** addition,activating transcription factor 3 was significantly upregulated,indicating nerve damage in the LM-MP of 24-day-old *** transcriptomic results showed that most of the differentially expressed genes in the ileal LM-MP after weaning were downregulated and closely related to the cell cycle *** RT-q PCR analysis showed that the relative expression of p21 was upregulated,while the expression of cyclin A2,cyclin B1,and proliferating cell nuclear antigen was downregulated in the ileal and colonic LM-MP of 24-day-old piglets,suggesting that weaning may inhibit cell proliferation and cause G1/S cell cycle arrest in ileal and colonic *** conclusion,weaning may lead to cell cycle arrest by causing DNA damage in the LM-MP,impairing i

关键词： weaning piglets diarrhea longitudinal muscle-myenteric plexus

Identification of pathological characteristics in pulmonary tuberculosis using polarization-sensitive optical coherence tomography

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Journal of Innovative Optical Health Sciences 2024年第6期17卷 97-106页

作者： Yun Ding Zhuoqun Yuan Xiaojiang Zhao Guozheng Gao Xin li Yanmei liang Clinical School of Thoracic Tianjin Medical University TianjinP.R.China Department of Thoracic Surgery Fujian provincial Hospital FujianP.R.China institute of Modern Optics Nankai University Tianjin Key Laboratory of Micro-scale Optical Information Science and Technology Tianjin 300350P.R.China School of medicine Tianjin University TianjinP.R.China Department of Pathology Tianjin Chest Hospital No.261Taierzhuang South Road Jinnan District Tianjin 300222P.R.China Department of Thoracic Surgery Tianjin Chest Hospital TianjinP.R.China

The low detection rate of Mycobacterium tuberculosis in clinical practice leads to a high rate of missed diagnosis for pulmonary tuberculosis(PTB).As a noninvasive,high-resolution,real-time imaging technology,polarization-sensitive optical coherence tomography(PS-OCT)may be feasible for the rapid identification of pathological *** study aimed to explore the feasibility of using PS-OCT to identify pathological features of *** the experiments,PTB samples containing some surrounding lung tissues were imaged using *** is demonstrated that PS-OCT images showed good consistency with the corresponding pathological images and were able to identify PTB-related characteristic pathological *** think PS-OCT can serve as an effective supplement for the diagnosis of PTB,enabling rapid and accurate diagnosis,and aiding in the understanding of the pathological characteristics and pathophysiological processes of PTB.

关键词： Pulmonary tuberculosis PS-OCT pathological features.