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Strain Cultivation Stage and Infectious Concentration on Soybean Cotyledonary Node via Agrobacterium–mediated Transformation System

2.10 IEEE 2.d Symposium on Web Society

作者： Kan Shi,Wenting Dai graduate university of {2. academy of sciences Zhaoli Song,Jinlong Zhu National university of Singapore Bo Wang Shanghai Polytechnic university Jifa Gu,Shanying Xu academy of Mathematics and Systems Science, {2. academy of sciences

＜正＞The paper analyzes the attributes and properties of the emerging hybrid *** changes that the hybrid network has brought to information dissemination and the development of the social collective behaviors,as well as the challenges it has imposed on the management of the social clusters are *** the previous researches based on network technologies,a new paradigm for hybrid network collective behaviors study has been proposed. The paper describes the preliminary framework of the volunteers’ investigation and the queue problem study in Expo for exploiting the inner mechanisms and development laws of the collective behaviors in hybrid *** results and practical suggestions about advancing management for enhancing volunteers ’ service quality are provided.

关键词： hybrid network social collective behaviors SMS computer questionnaire video surveillance system

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Journal of Northeast Agricultural university(English Edition) 2010年第2期17卷 1-6页

作者： WU Xiaoxia 1,LIU Weiting 2.LI Jing 1,LIU Shanshan 1,WANG Zhikun 1,and LI Wenbin 1 1 College of Agriculture,Northeast Agricultural university,chinese Education Ministry and Provincial Key Laboratory of Soybean Biology,Harbin 150030,{2. 2.Heilongjiang academy of Agricultural sciences,Harbin 150086,{2. College of Agriculture Northeast Agricultural UniversityChinese Education Ministry and Provincial Key Laboratory of Soybean BiologyHarbin 150030China Heilongjiang academy of Agricultural sciences Harbin 150086China

In soybean cotyledonary transformation,Agrobacterium infection is influenced by many *** cultivation stage of Agrobacterium strain and infectious concentration were studied in this *** showed that the highest rate of resistant shoots was obtained when the strain cultivation concentration was OD 600 =0.6,and the infectious concentration was OD 600 =*** soybean cultivars in North china were also screened for high susceptibility to Agrobacterium and three genotypes were found to be s...

关键词： soybean cotyledonary node Agrobacterium tumefaciens strain cultivation stage infectious concentration

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虎纹捕鸟蛛毒素Ⅴ的二硫键定位分析

生物化学与生物物理进展 2004年第6期31卷 550-555页

作者：张鹏飞肖顺勇梁宋平中南大学湘雅医院卫生部肿瘤蛋白质组学重点实验室长沙410008 湖南师范大学生命科学学院

虎纹捕鸟蛛毒素Ⅴ是从虎纹捕鸟蛛毒液中分离得到的一种昆虫毒素 .它含有 35个氨基酸残基 ,其中 6个半胱氨酸形成三对二硫键 .首先采用多酶将天然的肽链裂解后 ,通过MALDI TOF质谱分析酶解肽段 ,推断出 1对二硫键位于Cys9 Cys2.1,然后利... 详细信息

虎纹捕鸟蛛毒素Ⅴ是从虎纹捕鸟蛛毒液中分离得到的一种昆虫毒素 .它含有 35个氨基酸残基 ,其中 6个半胱氨酸形成三对二硫键 .首先采用多酶将天然的肽链裂解后 ,通过MALDI TOF质谱分析酶解肽段 ,推断出 1对二硫键位于Cys9 Cys2.1,然后利用改进的部分还原分步测序法 ,确定虎纹捕鸟蛛毒素Ⅴ的另外 2.二硫键的配对方式为Cys2. Cys16和Cys15 Cys2.8.因此 ,虎纹捕鸟蛛毒素Ⅴ的 3对二硫键分别以Cys2. Cys16 ,Cys9 Cys2.1,Cys15 Cys2.8(即 1 4、 2. 5和 3 6 )的方式配对 .

关键词：虎纹捕鸟蛛毒素V 二硫键定位三羧甲基磷酸(TCEP)

Detection of CD2.expression in chicken hematogenic embryo yolk sac lymphoid cells prior to thymus genesis

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Progress in Natural Science:Materials International 2008年第12期18卷 1589-1594页

作者： Dongyu Zhou~a Jigui Wang~b Weiquan Liu~b Rongxiu Liu~c Yuehu Pei~a a College of Traditional chinese Materia Medica,Shenyang Pharmaceutical university,Shenyang 110016,{2. b College of Biological sciences,{2. Agricultural university,Beijing 100094,{2. c Liaoning Tianhe Biotechnology Co.Ltd,Room 12.3,100-1 Dongbinhe Road,Shenyang District,Shenyang 110016,{2. [a]College of Traditional chinese Materia Medica Shenyang Pharmaceutical University Shenyang 110016 China [b]College of Biological sciences China Agricultural University Beijing 100094 China [c]Liaoning Tianhe Biotechnology Co. Ltd Room 1203 100-1 Dongbinhe Road Shenyang District Shenyang 110016 China

Lymphoid mononuclear cells from chick embryos at stage 16 were collected prior to fetal liver and thymus genesis to study the dif- ferentiation and function of the hematogenic yolk sac and to detect whether CD2.occurs on the surface of lymphoid mononuclear cells. The phenotype and functional activity of the cell surface protein E receptor and the ultrastructure of embryonic E~+ cells were compared with those of mature T *** results indicate 99.36% homology between the E receptors of embryonic lymphocytes and mature T *** similarities,including molecular distribution,motivation,the ability to form an erythrocyte rosette,the structure of the receptor-ligand complex,and the conformation of the signal channel,were detected between embryonic lymphocytes and mature CD2.expressing T *** results indicate that CD2.is already expressed prior to fetal liver and thymus genesis and that its expression is not dependent on the thymic microenvironment.

关键词： CD2 Chicken embryo Hematogenic yolk sac

Finite Volume Methods for Solving Electromagnetic Problems in Nonhomogeneous Media

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Wuhan university Journal of Natural sciences 2003年第S1期8卷 176-182页

作者： Zou JunDepartment of Mathematics. The chinese university of Hong Kong, Shatin, N. T. , Hong Kong, {2.. The chinese university of Hong Kong Department of Mathematics Shatin N. T Hong Kong China (GRID:grid.10784.3a) (ISNI:***)

This article shall review some of the recent advances on finite volume methods for solving electromagnetic problems in nonhomogeneous media. The stability, convergence and applications of the finite volume methods wil... 详细信息

关键词： Maxwell equations finite volume methods stability convergence

Blocking　effect　of　tanshinone　Ⅱ－Aon　L－type　Ca2.　curent　of　single　ventricular 　myocyte　from　guinea　pig

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中国药理学与毒理学杂志 1996年第2期 2-5页

作者： XU Chang-Qing, FAN Jin-Song 2. HAO Xue-Mei 2. ZHOU Ying-Ying 2. WANG Xiao-Ming, LIU Tai-Feng 2. (Department of Pathophysiology, Harbin Medical university, Harbin 150086 2.aboratory of Biological Membrane and Membrane Engineering, Departm

本实验采用全细胞膜片钳技术，观察了丹参酮Ⅱ－Ａ对分离的豚鼠单个心室肌细胞Ｌ－型钙电流及跨膜电位的影响．使用Ｌａｎｇｅｎｄｏｒｆ装置和蛋白酶循环消化（０．２ｇ·Ｌ－１）的方法，获取了７０％～８０％的横纹清晰，呈杆状的耐... 详细信息

本实验采用全细胞膜片钳技术，观察了丹参酮Ⅱ－Ａ对分离的豚鼠单个心室肌细胞Ｌ－型钙电流及跨膜电位的影响．使用Ｌａｎｇｅｎｄｏｒｆ装置和蛋白酶循环消化（０．２ｇ·Ｌ－１）的方法，获取了７０％～８０％的横纹清晰，呈杆状的耐钙心肌细胞．并使用ｐＣＬＡＭＰ５．５１微机程序和ＥＬＰ－７膜片钳放大器记录心肌细胞膜电流和跨膜电位的变化．丹参酮采用累积加药法，所有的观察指标均在同一细胞完成．数据分析采用配对ｔ检验．当使心肌细胞的保持电压为－４０ｍＶ，除极到０ｍＶ，刺激电压的时程为２５０ｍｓ，频率为０．５Ｈｚ时，１０，２０和４０μｍｏｌ·Ｌ－１的丹参酮Ⅱ－Ａ可使维拉帕米敏感的钙内向电流分别减少３５．２％，５７．７％和７４．７％（Ｐ＜０．０５），并能使动作电位时程亦呈剂量依赖性缩短，冲洗后两指标均部分恢复．该药的三种不同浓度，对不同电压最大激活的钙电流的电压依赖曲线均无影响．结果表明，丹参酮Ⅱ－Ａ具有类似维拉帕米样Ｌ－型钙通道阻断剂作用，其阻断作用呈非电压依赖性．

关键词：丹参酮Ⅱ-A L-型钙电流动作电位膜片钳心肌

Association and linkage analysis of COLIA1 and AHSG gene polymorphisms with femoral neck bone geometric parameters in both Caucasian and chinese nuclear families

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Acta Pharmacologica Sinica 2007年第3期 375-381页

作者： Hui JIANG~(2.3,5) Shu-feng LEI~(2.5) Su-mei XIAO~2.Yuan CHEN~(2.3) Xiao SUN~2.Fang YANG~(2.3) Li-ming LI~2.Shan WU~2.Hong-wen DENG~(2.4,6)2.Laboratory of Molecular and Statistical Genetics and the Key Laboratory of Protein Chemistry and Developmental Biology of the Ministry of Education,College of Life sciences,Hu-nan Normal university,Changsha 410081,{2. 3 Osteoporosis Research Center,Creighton university,Omaha 68131,USA 4 Department of Orthopedic Surgery,School of Medicine,university of Missouri-Kansas City,Kansas City 64108,USA

Aim:To simultaneously investigate the contribution of the alpha 1 chain of col-lagen type 1(COLlAl)and alpha2.HS-glycoprotein(AHSG)genes to the varia-tion of bone geometric parameters in both Caucasians and ***:Sixhundred and five Caucasian individuals from 157 nuclear families and 12.8 Chi-nese subjects from 400 nuclear families were genotyped at the AHSG-Sacl,COLIA1-PCOL2.and Spl polymorphisms using polymerase chain reaction(PCR)-restric-tion fragment length polymorphism(RFLP).5 FN bone geometric parameters werecalculated based on bone mineral density and bone area of femoral neck(FN)measured by dual energy X-ray *** stratification,totalfamily association,within-family association,and linkage tests were performed bythe quantitative transmission disequilibrium test ***:The t-testshowed the significant differences of all bone geometric phenotypes(except ED)between Caucasians and chinese in the offspring using both unadjusted andadjusted(by age,height,weight,and gender)*** Caucasians,we foundsignificant within-family association results between the COLlAl-Spl polymor-phism(rs1800012.and cross sectional area(CSA),cortical thickness(CT),endocortical diameter(ED),buckling ratio(BR)(P=0.018,0.002.0.02.,and 0.001,respectively);the COLlAl-Spl polymorphism also detected significant linkagewith BR(P=0.039).In the population of china,the within-family associationsbetween the COLlAl-PCOL2.polymorphism(rs1107946)and CT,BR were signifi-cant(P=0.012.and 0.008,respectively).Furthermore,evidence of linkage wereobserved between the AHSG-Sacl polymorphism(rs4918)and CT,BR(P=0.042.nd 0.014,respectively)in Caucasians,but not in ***:Ourresults suggest that the COLlAl gene may have significantly association withbone geometry in both Caucasians and chinese,and the AHSG gene may belinked to bone geometry in Caucasians,but not in *** study representsour first efforts on investigating the importance of the COLlAl and A

关键词： COLIA1 AHSG bone geometry transmission disequilibrium test

microRNAs in early zebrafish development:from gastrulation cell movements to craniofacial skeleton

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microRNAs in early zebrafish development:from gastrulation c...

Non-integrable Movement Equations in Landsliding System

2.12.国发育生物学大会

作者： Guozhu Ning~(1*),Xiuli Liu~(1*),Anming Meng~(1,2.,Qiang Wang~1 1 State Key Laboratory of Biomembrane and Membrane Biotechnology,Institute of Zoology,chinese academy of {2., Beijing,china 2.Tsinghua-Peking Center for Life {2.,School of Life {2.,Tsinghua university,Beijing,china

The regulation of gene expression by microRNAs(miRNAs) plays a critical role in different cellular processes, including metabolism,apoptosis,differentiation,and *** of miRNA biogenesis by the loss of dicer in zebrafish maternal-zygotic dicer(MZdicer) mutants,causes abnormal morphogenesis during gastrulation,and reduces pharyngeal cartilage at later ***,the functional role and molecular mechanisms of miRNAs in regulating theses important developmental processes have not been ***,we show that mir2.6 is essential for normal gastrulation cell movements by regulating JNK2.phosphorylation through inhibition of prickle1a expression and mir92. acts to maintain Bmp activity during craniofacial skeleton formation by targeting ***,more or less amount of these miRNAs results in similar developmental defects,implying that an appropriate expression level of these miRNAs are required during early zebrafish development,from gastrulation cell movements to craniofacial skeleton. Conclusions:Our data discover that mir2.6 targets pk1a to modulate JNK2.activation during zebrafish gastrulation and mir92.-mediated regulation of nog3 is critical for ingeniously balancing Bmp signal activity during pharyngeal cartilage formation.

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Journal of Earth Science 1999年第4期18卷 322-326页

作者： Yan Tongzhen(Faculty of Engineering, china university of Geo{2., Wuhan 430074)Wang Jianfeng(LNM, Institute of Mechanics, chinese academy of {2., Beijing 100080)Yan Lei(Beijing university of Aeronautics and Astronautics, Beijing 100083)Zhou Cuiyi Faculty of Engineering China University of GeosciencesWuhan 430074 LNM Institute of MechanicsChinese Academy of SciencesBeijing 100080 Beijing university of Aeronautics and Astronautics Beijing 100083 Department of Earth sciences Zhongshan UniversityGuangzhou 510275

The answer of the question was considered as non-integrable basically. The integrable system was thought in the *** Poincare had presented in a theorem and proved that non-integrable system is normal, and yet integrable system is abuormal. In the landsliding system, discussed question was regarded to be *** fundamentals were considered; other systems and example with engineering control are concerned and discussed in details.

关键词： non-integrable equation landslide engineering geology