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Mass Spectrometric Evidence of Heparin Disaccharides for the Catalytic Characterization of A Novel Endolytic Heparinase

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Acta Biochimica et Biophysica Sinica 2004年第12期36卷 840-844页

作者： Yapeng CHAO1,3, Shaoxiang XIONG2. Xiulan CHENG1, and Shijun QIAN1* 1Institute of Microbiology, chinese academy of {2., beijing 100080, China 2.enter of Mass Spectrometry, Institute of Chemistry, chinese academy of {2., beijing 100080, China 3graduate School of the chinese academy of {2.,{12., China Institute of Microbiology Chinese Academy of SciencesBeijing 100080 China graduate School of the chinese {2. of sciencesbeijing 100039 China Center of Mass Spectrometry Institute of Chemistry Chinese Academy of SciencesBeijing 100080 China

Heparinase from different sources can eliminate heparin or/and heparan sulfate into various low-molecular weight heparins with different characteristics. Porcine intestinal mucosa heparin was de- graded into a series of oligosaccharides by a novel heparinase from the species Sphingobacterium. Disaccha- ride components from the digests were separated and purified by ultrafiltration and HPLC. Five major peaks appeared as three types according to their retention time. The mass spectrometry of peak I mainly gave the non-sulfated disaccharide with the mass of 379 Da. Peak II and III were indicated as two major monosulfated disaccharides with molecular mass of 417 and 459 Da respectively. Moreover, the peak III represented an N- acetyl disaccharide. Both peak IV and V showed the same mass of 496 Da, hinting that they were disulfate- substituted disaccharides. No trisulfate-substituted disaccharides were detected in the mixture of the heparin digest though they were abundant in the heparin structure. The results revealed that the heparinase might specifically cut the sites with low sulfated domain in heparin.

关键词： electrospray ionization-mass spectrometry heparin disaccharides heparinase high perfor- mance liquid chromatography Sphingobacterium sp.

Possible Energy Network with polygeneration system and CCS for China

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中国科学：技术科学英文版 2010年第1期53卷 33-39页

作者： GAO Lin1,LI Sheng1,2.JIN HongGuang1 & LIN Hu1 1 Institute of Engineering Thermophysics,chinese academy of {2.,beijing 100190,China 2.graduate School of chinese academy of {2.,beijing 100039,China 1. Institute of Engineering Thermophysics Chinese Academy of Sciences Beijing 100190 China2. Graduate School of Chinese Academy of Sciences Beijing 100039 China

On basis of adopting polygeneration systems for power and alternative fuels,capturing CO2.with near zero energy penalties,and storing CO2.on sites,a new kind of Energy Network can integrate energy utilization,CO2.capture,transportation and storage ***-economic analysis of this solution focusing on Inner Mongolia and the Yangtze River Delta districts had been carried with comparison to the chain method for energy utilization and CO2.*** solution can save 2..5% of energy,and reduce 35% of total *** adoption of advanced polygeneration systems contributes 52.2. of the total saved costs,and the integration of energy utilization and CO2.sequestration in the Energy Network contributes 47.8%.From the aspect of CCS,the CO2.sequestration cost in the Energy Network can be as low as 12.$/t due to the lower energy penalties of capture in polygeneration systems and the combination of CO2.source and *** Energy Network exhibits attractive performance on energy saving,costs reduction for CCS,which may be a promising solution for sustainable development of China.

关键词： CO2 capture and storage Energy Network polygeneration system

Synthesis of well-defined functional PE graft copoly-mers via ATRP process

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Science Bulletin 2005年第10期50卷 1048-1051页

作者： CAO Chengang1, 2. DONG Jinyong1 & HU Youliang1 1. CAS Key Laboratory of Engineering Plastics, Joint Laboratory of Polymer Science and Materials, Institute of Chemistry, chinese academy of {2., beijing 100080, China 2. graduate School, chinese academy of {2., beijing 100039, China CAS Key Laboratory of Engineering Plastics Joint Laboratory of Polymer Science and Materials Institute of Chemistry Chinese Academy of Sciences Beijing 100080 China graduate School Chinese Academy of Sciences Beijing 100039 China

In summary, we have demonstrated a convenient yet highly efficient method forthe preparation of PE-based graft copolymers via ATRP.. By introducing a small amount of styrenegroups into PE chains via the selective copolymerization of ethylene with 1,4-divinylbenzenecatalyzed by Et(Ind)_2.rCl_2.MAO, the multifunctional ATRP initiator of1-chloroethylbenzene-containing PE was readily achieved by a quantitative hydrochlorination reactionand was shown to be very effective for the initiation of graft polymerization of MMA and styrene toprepare structurally well-defined PE-based graft copolymers.

关键词： ATRP Synthesis of well-defined functional PE graft copoly-mers via ATRP process PE well

Artificial coordinating field and its application to motion planning of robots in uncertain dynamic environments

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Science China(Technological sciences) 2004年第5期47卷 577-594页

作者： JING Xingjian1, 2. WANG Yuechao1 & TAN Dalong1 1. Robotics Laboratory, Shenyang Institute of Automation, chinese academy of {2., Shenyang 110016, China 2. graduate School, chinese academy of {2., beijing 100039, China Correspondence should be addressed to Jing Xingjian 1. Robotics Laboratory Shenyang Institute of Automation Chinese Academy of Sciences 10016 Shenyang China 2. graduate School Chinese Academy of Sciences 10039 Beijing China

Artificial coordinating fields (ACF) are proposed to deal with the motion planning problems of mobile robots in uncertain dynamic environments. An ACF around an obstacle can generate two orthogonal force vectors to a robot: one is called the coordinating force vector which is purposively designed in this paper, and the other is the repulsive force vector which is the same as that in a conventional artificial potential field. The ACF is designed according to the updated motion purpose and the relative states of the robot with respect to its local environment, and it also satisfies the robot’s dynamic constraints. The direction of the coordinating force can be determiend on line according to an optimal evaluation function. The ACF can effectively remove the local minima, and reduce the oscillation of the planned trajectory between multiple obstacles. Only local knowledge of the environments is needed in the ACF-based motion planning. The properties of the ACF such as controllability, adaptability, safety and reachability are studied and discussed in detail in this paper. Theoretical analysis and simulations are given to illustrate our main results.

关键词： artificial coordinating fields, artificial potential fields, mobile robots, motion planning, uncertain dynamic environments.

Cloning and expression of putative ethylene receptor genes in soybean plant

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Progress in Natural Science:Materials International 2007年第10期17卷 1152-1160页

作者： Xie Zongming1,2.3, Lei Gang1,2. Wuriyanghan Hada1,2. Tian Aiguo1, Zhang Jinsong1 and Chen Shouyi1(1. National Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, chinese academy of {2., beijing 100101, China 2. graduate School of chinese academy of {2., beijing 100039, China 3. Xinjiang academy of Agricultural and Reclamation Science, Shihezi 832.00, China) National Key Laboratory of Plant Genomics Institute of Genetics and Developmental Biology Chinese Academy of Sciences Beijing 100101 China graduate School of chinese {2. of sciences Beijing 100039 China Xinjiang academy of Agricultural and Reclamation Science Shihezi 832000 China

Ethylene plays important roles in plant growth, development, and stress responses, and ethylene receptors have been identified and studied extensively in various plant species. Here we report the cloning of four ethylene receptor genes from soybean, i.e. GmETR1, GmERS1, GmETR2.and GmEIN4. Construction of the phylogenic tree showed that GmETR1 and GmERS1 belong to subfamily I whereas GmETR2.and GmEIN4 belong to subfamily II. The four ethylene receptor genes showed different tissue-specific expression patterns in roots, stems, leaves, cotyledons, flowers, pods and seeds of soybean. These genes were differentially regulated by various abiotic stresses and plant hormones. The possible roles of the four genes in soybean plant were also discussed.

关键词： abiotic stress expression analysis ethylene receptor plant hormone soybean.

Population information extraction in Chaohu watershed based on RS and GIS

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chinese Science Bulletin 2003年第2期48卷 181-183页

作者： LIU Jianjun1,2.& LI Chunlai3 1. Institute of Geochemistry, chinese academy of {2., Guiyang 550002. China 2. graduate School, chinese academy of {2., beijing 100039, China 3. National Astronomical Observatories, chinese academy of {2., beijing 100012. China Correspondence should be addressed to Liu Jianjun (e-mail: dhsliujj@ ***) Institute of Geochemistry Chinese Academy of Sciences Guiyang China graduate School Chinese Academy of Sciences Beijing China National Astronomical Observatories Chinese Academy of Sciences Beijing China

It is always difficult to extract population in-formation of small watershed during the region environment assessment. The report adopted compound areal interpola-tion to study the population in Chaohu watershed with the GIS technique and landuse interpretation data from remote sensing. The result indicated that the method is effective to extract population information of small watershed.

关键词：巢湖流域人口分布信息提取 RS GIS 小流域生态环境评价

Characteristics of chlorine isotope distribution and analysis on sylvinite deposit formation based on ancient salt rock in the western Tarim Basin

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Science China Earth sciences 2005年第11期48卷 1913-1920页

作者： TAN Hongbing1,2. MA Haizhou1, XIAO Yingkai1, WEI Haizhen1,2. ZHANG Xiying1 & MA Wandong1 1. Institute of Salt Lakes, chinese academy of {2., Xining 810008, China 2. graduate School of the chinese academy of {2., beijing 100039, China 1. Institute of Salt Lakes Chinese Academy of Sciences 810008 Xining China 2. graduate School of the chinese {2. of sciences 100039 Beijing China

During formation of the Tarim Basin, there repetitively happened seawater invasion for it was ever connected with wide Paleo-mediterranean Sea. At the same time, for the applica-tion of tectonic and paleoenvironmental condition, the basin deposits many sequences of thick strata of evaporites, especially in its two largest subbasins of Kuqa in the north and Shaqa in the southwest. Therefore, the western Tarim Basin has been considered to be a favorable location to prospect ancient sylvinite deposit. But it is unfortunate that the Br×103/Cl ratio, as a common geochemical index to uncover clues of sylvinite deposit, is almost invalid to indicate the stage of ancient brine condensation for very low content of bromine in salt rock samples (less than 10?6 commonly) in the western Tarim Basin evaporites. Fortunately, we noticed recently that the val-ues of chlorine isotope (δ 37Cl value) for salt rock samples follow some clear laws from place to place, which in the Shaqa subbasin, all δ 37Cl values of salt rock samples behaved negatively and on the contrary, they all surprisingly showed positive values in the Kuqa subbasin. Based on conclusions of previous research and referring to the ratios of Br×103/Cl for salt rock or brine samples, we proved that the chlorine isotope (δ 37Cl) could be used as an effective index to find ancient sylvinite deposit in those evaporites rock basin with low content of Br element back-ground, such as the Tarim Basin. Thus according to the new index of chlorine isotope, we con-clude that the concentration of ancient brines formed ancient salt rocks is much more concen-trated in the Shaqa subbasin than that in the Kuqa subbasin. Particularly, the δ 37Cl value of salt rock in Kashi depression of the western Shaqa subbasin is greatly negative (

关键词： the Tarim Basin, chlorine isotope, ancient salt rock, ancient sylvinite deposit, deposit stage

Recent advances in the data analysis method of functional magnetic resonance imaging and its applications in neuroimaging

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Progress in Natural Science:Materials International 2006年第8期16卷 785-795页

作者： TIAN Jie, YANG Lei and HU Jin (Institute of Automation, chinese academy of {2., beijing 100080, China graduate School of chinese academy of {2., beijing 100039, China) [a]chinese academy of {2. Institute of Automation Beijing China [b]graduate School of chinese {2. of sciences Beijing China

Functional magnetic resonance imaging (fMRI) has opened a new area to explore the human brain. The fMRI can reveal the deep insights of spatial and temporal changes underlying a broad range of brain function, such as motor, vision, memory and emotion, all of which are helpful in the clinical investigation. In this paper, we introduce some recent-developed algorithms for fMRI signal detection such as model-driven method (general linear model, deconvolution model, non-linear model, etc. ) and data-driven method (principle component analysis, independent component analysis, self-organization mapping, clustered constrained non-negative matrix factorization, etc. ). We also propose several important applications of neuroimaging and point out their shortcomings and future perspectives.

关键词： fMR1 data analysis neuroimaging.

Overexpression of a tobacco small G protein gene NtRop1 causes salt sensitivity and hydrogen peroxide production in transgenic plants

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Science China(Life sciences) 2008年第5期51卷 383-390页

作者： CAO YangRong1,2. LI ZhiGang1,2. CHEN Tao1,2. ZHANG ZhiGang1, ZHANG JinSong1 & CHEN ShouYi1 1 National Key Lab of Plant Genomics, Institute of Genetics and Developmental Biology, chinese academy of {2., beijing 100101, China 2.graduate School of chinese academy of {2., beijing 100039, China 1. National Key Lab of Plant Genomics Institute of Genetics and Developmental Biology Chinese Academy of Sciences Beijing 100101 China 2. graduate School of chinese {2. of sciences Beijing 100039 China

The small GTPases of Rop/Rho family is central regulators of important cellular processes in plants. Tobacco small G protein gene NtRop1 has been isolated; however, its roles in stress responses were unknown. In the present study, the genomic sequence of NtRop1 was cloned, which has seven exons and six introns, similar to the Rop gene structure from Arabidopsis. The NtRop1 gene was constitutively expressed in the different organs whereas the other six Rop genes from tobacco had differential expression patterns. The expression of the NtRop1 gene was moderately induced by methyl viologen, NaCl, and ACC treatments, but slightly inhibited by ABA treatment, with no significant induction by NAA treatment. The transgenic Arabidopsis plants overexpressing the NtRop1 showed increased salt sensitivity as can be seen from the reduced root growth and elevated relative electrolyte leakage. The hydrogen peroxide production was also promoted in the NtRop1-trangenic plants in comparison with wild type plants. These results imply that the NtRop1 may confer salt sensitivity through activation of H2.2.production during plant response to salt stress.

关键词： small G protein NtRop1 salt sensitive hydrogen peroxide