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中国植物病理学会2012年学术年会

作者： XU Qiu-Fang~1 CHENG Wei-Shun~1 LI Shuang-Sheng~1 LI Wen~1 Zhang Zhi-Xin~1 XU You-Ping~2 CAI Xin-Zhong~(1**) (1.Institute of Biotechnology,College of Agriculture and Biotechnology,zhejiang university, 866 yu hang tang road,hangzhou 310058,china 2.center of analysis and measurement, zhejiang university,866 yu hang tang road,hangzhou 310058,china

＜正＞Identification of hypersensitive response（HR） regulators is essential to dissect the molecular mechanisms underlying plant disease *** this study,combined proteomics and RNA interfering analyses were employed to identify genes required for the HR conferred by the tomato resistance gene Cf-4.and the Cladosporium fulvum avirulence gene *** nine proteins differentially expressed in the tomato seedlings mounting and those not mounting the Cf-4.Avr4.dependent HR were identified through proteomics *** them were a variety of defence-related proteins including a cysteine protease Pipl,an operative target of another *** effector ***,glutathione-mediated antioxidation is a major response to the Cf-4.Avr4.dependent *** analysis through Tobacco rattle virus-induced gene silencing and transient RNAi assays of the chosen sixteen differentially expressed proteins revealed that seven genes,which encode Pipl homolog NbPipl,a SIPK type MAP kinase Nbf4.an asparagine synthetase NbAsn,a trypsin inhibitor LeMir-like protein NbMir,a small GTP- binding protein,a late embryogenesis-like protein and an ASR4.like protein,were required for the Cf- 4.Avr4.dependent ***,the former four genes were essential for the Cf-9/Avr9-dependent HR;NbPipl,NbAsn and NbMir but not NbfA affected a nonadaptive bacterial pathogen Xanthomonas oryzae ***-induced HR as well in Nicotiana *** data demonstrate that Pipl and LeMir may play a general role in HR and plant immunity,and application of combined proteomics and RNA interfering analyses is an efficient strategy to identify genes required for HR,disease resistance and probably other biological processes in plants.

关键词： Cf Cladosporium fulvum hypersensitive response defence gene silencing proteomics regulation resistance RNAi

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Multiwall Carbon Nano-Onions Induce DNA Damage and Apoptosis in Human Umbilical Vein Endothelial Cells

Multiwall Carbon Nano-Onions Induce DNA Damage and Apoptosis...

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3rd Asian Conference on Environmental Mutagens & 15th Conference of Chinese Environmental Mutagen Society

作者： yuying Xu~(1,2),Shuang-Ying Wang~(1,3),Jie Yang~1,Xiaomin Gu~1,Jun Zhang~1,Yi-Fan Zheng~1,Jun Yang~4.Lihong Xu~2,Xin-Qiang Zhu~1 1 Department of Toxicology,zhejiang university School of Public Health,{4. yu-hang-{4.road,hangzhou,zhejiang 310058,china 2 Department of Biochemistry and Genetics,zhejiang university School of Medicine,{4. yu-hang-{4. road,hangzhou,zhejiang 310058,china 3 Shangcheng center for Disease Control and Prevention,hangzhou,zhejiang 310009,china 4.Department of Toxicology,hangzhou Normal university School of Public Health,16 Xue Lin Street,hangzhou,zhejiang 310036,china

Growing evidence has indicated the potential adverse effects on cardiovascular system of some nanomaterials,including *** this study,we have evaluated the biological effects of multiwall carbon nano-onions(MWCNOs)(average size of 31.2 nm,ζ, potential of 1.6 mV) on human umbilical vein endothelial cells(HUVECs).It was found that MWCNOs exhibited a dose-dependent inhibitory effect on cell growth;EC(50) was 4..12μg/***,three concentrations were chosen(0.2,1,and 5μg/mL) for further *** cytometry analysis revealed that 1 and 5μg/mL MWCNOs could induce apoptosis in HUVECs,the apoptotic rates were 12%and 24.at 24.h after

关键词： HUVECs Multiwall Carbon Nano-Onions Induce DNA Damage and Apoptosis in Human Umbilical Vein Endothelial Cells DNA

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基于可达阵的多级评分最简完备Q矩阵设计

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心理学报 2024年第11期56卷 1634-1650,I0007-I0013页

作者：唐小娟彭志霞秦珊珊丁树良毛萌萌李瑜江西师范大学教育学院南昌330022 浙江工商大学统计与数学学院杭州310018 江西师范大学计算机信息工程学院南昌330022 南昌大学公共政策与管理学院南昌330036 浙江工商大学马克思主义学院心理健康教育中心杭州310018

Q矩阵的完备性是认知诊断模型具有可识别性的关键。多级评分含有比0-1评分更丰富的诊断信息,却鲜见多级评分完备Q矩阵的设计研究。用最少的题量获得最高判准率是测验设计者追求的目标,借鉴0-1评分完备Q矩阵的设计方法,本文提出从可达阵... 详细信息

Q矩阵的完备性是认知诊断模型具有可识别性的关键。多级评分含有比0-1评分更丰富的诊断信息,却鲜见多级评分完备Q矩阵的设计研究。用最少的题量获得最高判准率是测验设计者追求的目标,借鉴0-1评分完备Q矩阵的设计方法,本文提出从可达阵中获取多级评分结构化/非结构化最简完备Q矩阵(SSCQM/USCQM)的方法和算法。模拟实验得出以下结论:(1)测验含SSCQM/USCQM越多,判准率越高;(2)当列数相同时,含多个SSCQM或多个USCQM测验的判准率与含可达阵测验的判准率非常接近;(3)对于一些结构,纵使多个SSCQM/USCQM的列数少于可达阵列数,其判准率仍不低于可达阵。总之,短测验设计优先选择SSCQM;长测验设计优先选择USCQM。

关键词：多级评分测验设计结构化最简完备Q矩阵非结构化最简完备Q矩阵算法

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A Lipidomic Study of the Effects of N-methyl-N′-nitro-N-nitrosoguanidine on Sphingomyelin Metabolism

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Acta Biochimica et Biophysica Sinica 2005年第8期37卷 515-524页

作者： yun HUANG~(&#) Jing SHEN~# Ting WANG Yan-Ke yu Fanqing E CHEN~1 Jun YANG~* Department of Pathology and Pathophysiology,center for Environmental Genomics zhejiang university School of Medicine,{4.zhou 310031,china ~1 Molecular Biology Branch.Life Science Division,Lawrence Berkeley National Laboratory,{4. of California at Berkeley,Berkeley,CA 94.20,USA Department of Chemistry,Georgia State {4..Atlanta,GA 30303.USA DepartmentofPathologyandPathophysiology CenterforEnvironmentalGenomicsZheJiangUniversitySchoolofMedicineHang

Systems biology is a new and rapidly developing research area in which,by quantitativelydescribing the interaction among all the individual components of a cell,a systems-level understanding of abiological response can be ***,it requires high-throughput measurement technologies forbiological molecules,such as genomic and proteomic approaches for DNA/RNA and protein,***,a new concept,lipidomics,which utilizes the mass spectrometry(MS)method for lipid analysis,has been *** this lipidomic approach,the effects of N-methyl-N'-nitro-N-nitrosoguanidine(MNNG)on sphingomyelin metabolism,a major class of sphingolipids,were *** moleculeswere extracted from cells and analyzed by matrix-assisted laser desorption ionization-time of flight *** found that MNNG induced profound changes in sphingomyelin metabolism,including the appearance ofsome new sphingomyelin species and the disappearance of some others,and the concentrations of severalsphmgomyelin species also *** was accompanied by the redistribution of acid sphingomyelinase(ASM),a key player in sphingomyelin *** the other hand,imipramine,an inhibitor of ASM,caused the accumulation of *** also prevented some of the effects of MNNG,as well as theredistribution of *** together,these data suggested that the lipidomic approach is highly effectivefor the systematic analysis of cellular lipids metabolism.

关键词： lipidomics mass spectrometry ceramide sphingomyelin acid sphingomyelinase

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Numerical analysis of the influence of a river on tunnelling-induced ground deformation in soft soil

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Journal of zhejiang university-Science A(Applied Physics & Engineering) 2022年第7期23卷 564-578页

作者： Jia-xin LIANG Xiao-wu tang Tian-qi WANG yu-hang YE Ying-jing LIU Research center of Coastal and Urban Geotechnical Engineering Department of Civil EngineeringZhejiang UniversityHangzhou310058China Engineering Research center of Urban Underground Space Development of zhejiang Province Hangzhou310058China Zhongtian Construction Group Co. Ltd.Hangzhou310020China

When tunnels are constructed in coastal cities,they will inevitably undercross a *** the influence of rivers on tunnelling-induced deformation in costal soft soil is of great significance for controlling excessive settlement and protecting surrounding *** paper presents a case study of twin tunnels undercrossing a river in soft soil in hangzhou,*** soft soil of hangzhou refers to cohesive soil in a soft plastic or fluid plastic state with high natural water content,high compressibility,low bearing capacity,and low shear *** the influence of the river,the research region was divided into two parts,inside and outside the river-affected area,based on monitoring data of the Zizhi *** development law of surface settlement is divided into three *** the first and second stages,the surface settlement within and outside the river-affected area showed a similar trend:the settlement increased and the growth rate of settlement in the second stage was smaller within the river-affected *** the third stage,the surface settlement continued to increase within the river-affected area,while it converged outside the river-affected *** the river-affected area,there was an asynchronization of the sinking rate and stability of vault settlements and surface settlements.A numerical model was established by simulating different reinforcements of the *** numerical model revealed that the ground movement is influenced by the distribution and amount of the excess pore water *** excess pore pressure was concentrated mostly in the range of 1.0H_(t)-3.0H_(t)(H_(t) is the tunnel height)before the tunnel face,especially within the river-affected *** the river-affected area,the dissipation of excess pore water pressure needs more time,leading to slow stabilization of surface *** undercrossing a river,a cofferdam is necessary to reduce excessive ground deformation by dispersing the distribution o

关键词： Tunnelling Ground deformation Numerical analysis Reinforcement

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基于气体传感器和随机森林的毒害气体浓度检测方法研究

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传感技术学报 2020年第3期33卷 340-343页

作者：汤旭翔余智浙江工商大学管理工程与电子商务学院杭州310018 浙江工商大学网络信息中心杭州310018

研究了一种基于气体传感器和随机森林分析模型的毒害气体浓度检测方法,在温度25℃、相对湿度80%、标准大气压的条件下,实验模拟检测氨气、甲苯、硫化氢三种毒害气体泄漏并记录检测数据,采用非线性随机共振模型解析检测数据特征值,采用... 详细信息

研究了一种基于气体传感器和随机森林分析模型的毒害气体浓度检测方法,在温度25℃、相对湿度80%、标准大气压的条件下,实验模拟检测氨气、甲苯、硫化氢三种毒害气体泄漏并记录检测数据,采用非线性随机共振模型解析检测数据特征值,采用随机森林模型对检测特征值进行气体类型预测。研究结果表明,所提出的方法具有较好的预报精度。本文所提出的方法可以有效的解析检测传感器的信号特征向量,依托随机森林模型对于毒害气体泄漏浓度的判断更加精准,将在实验室危化品泄漏监测领域具有广阔的前景。

关键词：实验室安全毒害气体泄漏随机森林方差

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RALF22 promotes plant immunity and amplifies the Pep3 immune signal

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Journal of Integrative Plant Biology 2023年第11期65卷 2519-2534页

作者： yu-Han He Song-yu Chen Xing-Yan Chen You-Ping Xu Yan Liang Xin-Zhong Cai Key Laboratory of Biology of Crop Pathogens and Insects of zhejiang Province Institute of BiotechnologyCollege of Agriculture and BiotechnologyZhejiang University866 Yu Hang Tang RoadHangzhou 310058China Centre of analysis and measurement Zhejiang University866 Yu Hang Tang RoadHangzhou 310058China Hainan Institute Zhejiang UniversitySanya 572025China

Rapid alkalinization factors(RALFs)in plants have been reported to dampen pathogenassociated molecular pattern(PAMP)-triggered immunity via suppressing PAMP-induced complex formation between the pattern recognition receptor(PRR)and its co-receptor ***,the direct and positive role of RALFs in plant immunity remains largely ***,we report the direct and positive roles of a typical RALF,RALF22,in plant ***22alone directly elicited a variety of typical immune responses and triggered resistance against the devastating necrotrophic fungal pathogen Sclerotinia sclerotiorum in a FERONIA(FER)-dependent ***(LRE)-like glycosylphosphatidylinositol(GPI)-anchored protein 1(LLG1)and NADPH oxidase RBOHD were required for RALF22-elicited reactive oxygen species(ROS)*** mutation of cysteines conserved in the C terminus of RALFs abolished,while the constitutive formation of two disulfide bridges between these cysteines promoted the RALF22-elicited ROS production and resistance against ***,demonstrating the requirement of these cysteines in the functions of RALF22 in plant ***,RALF22 amplified the Pep3-induced immune signal by dramatically increasing the abundance of PROPEP3 transcript and *** with RALF22 induced resistance against *** in Brassica crop ***,our results reveal that RALF22 triggers immune responses and augments the Pep3-induced immune signal in a FER-dependent manner,and exhibits the potential to be exploited as an immune elicitor in crop protection.

关键词： RALF22 FER reactive oxygen species Pep3 plant immunity Sclerotinia sclerotiorum

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Structural Domain Imaging and Direct Determination of Crystallographic Orientation in Noncentrosymmetric Ca3 Ru2O7 Using Polarized Light Reflectance

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Chinese Physics Letters 2020年第10期37卷 43-47页

作者： Guoxiong tang Libin Wen Hui Xing Wenjie Liu Jin Peng yu Wang yupeng Li Baijiang Lv yusen Yang Chao Yao yueshen Wu Hong Sun Zhu-An Xu Zhiqiang Mao Ying Liu Key Laboratory of Artificial Structures and Quantum Control and Shanghai Center for Complex PhysicsSchool of Physics and AstronomyShanghai Jiao Tong UniversityShanghai 200240China Department of Physics and Engineering Physics Tulane UniversityNew Orleans 70118USA Department of Physics Pennsylvania State UniversityUniversity ParkPA 16802USA zhejiang Province Key Laboratory of Quantum Technology and Device Department of PhysicsZhejiang UniversityHangzhou 310027China

The noncentrosymmetricity of a prototypical correlated electron system Ca3Ru2O7 renders extensive interest in the possible polar metallic state,along with multiple other closely competing ***,the structural domain formation in this material often complicates the study of intrinsic material *** is crucial to fully characterize the structural domains for unrevealing underlying ***,we report the domain imaging on Ca3Ru2O7 crystal using the reflection of polarized light at normal *** reflection anisotropy measurement utilizes the relative orientation between electric field component of the incident polarized light and the principal axis of the crystal,and gives rise to a peculiar *** domain walls are found to be the interfaces between 90° rotated twin crystals by complementary magnetization measurements.A distinct contrast in reflectance is also found in the opposite cleavage surfaces,owing to the polar mode of the RuO6 *** importantly,the analysis of the contrast between all inequivalent cleavage surfaces enables a direct determination of the crystallographic orientation of each *** an approach provides an efficient yet feasible method for structural domain characterization,which can also find applications in noncentrosymmetric crystals in general.

关键词： symmetric cleavage utilize

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A highly sensitive luminescent metal-organic framework thermometer for physiological temperature sensing

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Journal of Rare Earths 2018年第6期36卷 561-566页

作者： Lin Zhang Yadan Xie Tifeng Xia yuanjing Cui yu Yang Guodong Qian State Key Laboratory of Silicon Materials Cyrus Tang Center for Sensor Materials and ApplicationsSchool of Materials Science and EngineeringZhejiang University

Thermal sensing and imaging in the physiological temperature range are of great importance for studying physiological processes and treating diseases. Metal-organic frameworks(MOFs) exhibit great promise for developing luminescent thermometers due to their remarkable structural diversities and tunable luminescence properties. Here, we synthesized a series of luminescent mixed-lanthanide MOFs,EuTbBPT(x = 0.019, 0.058, 0.106; H3 BPT = biphenyl-3,4.,5-tricarboxylate acid) and adopted powder X-ray diffraction(PXRD), thermogravimetric analysis(TGA) and Fourier transform infrared(FT-IR) to characterize the resulting products. The temperature-dependent photoluminescence emission spectra were recorded to investigate their potential applications in physiological temperature readout. It is found that the intensity ratio of Tbto Euis linearly correlated with temperature and the relative sensitivity is higher than 1.5%/℃ over the entire physiological temperature range. Furthermore,the temperaturedependent luminescence color emission allows for visual colorimetric temperature *** lifetime testing and triplet energy level measurement were further conducted to study the mechanism.

关键词： Lanthanide metal-organic frameworks Luminescent thermometer Ratiometric Colorimetric High sensitivity Physiological temperature

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CEPC Technical Design Report

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Radiation Detection Technology and Methods 2024年第1期8卷 I0003-I0016,1-1091页

作者： Waleed Abdallah Tiago Carlos Adorno de Freitas Konstantin Afanaciev Shakeel Ahmad Ijaz Ahmed Xiaocong Ai Abid Aleem Wolfgang Altmannshofer Fabio Alves Weiming An Rui An Daniele Paolo Anderle Stefan Antusch Yasuo Arai Andrej Arbuzov Abdesslam Arhrib Mustafa Ashry Sha Bai yu Bai Yang Bai Vipul Bairathi Csaba Balazs Philip Bambade Yong Ban Tripamo Bandyopadhyay Shou-Shan Bao Desmond P.Barber Ayse Bat Varvara Batozskaya Subash Chandra Behera Alexander Belyaev Michele Bertucci Xiao-Jun Bi yuanjie Bi Tianjian Bian Fabrizio Bianchi Thomas Biekotter Michela Biglietti Shalva Bilanishvili Deng Binglin Denis Bodrov Anton Bogomyagkov Serge Bondarenko Stewart Boogert Maarten Boonekamp Marcello Borri Angelo Bosotti Vincent Boudry Mohammed Boukidi Igor Boyko Ivanka Bozovic Giuseppe Bozzi Jean-Claude Brient Anastasiia Budzinskaya Masroor Bukhari Vladimir Bytev Giacomo Cacciapaglia Hua Cai Wenyong Cai Wujun Cai Yijian Cai Yizhou Cai yuchen Cai Haiying Cai Huacheng Cai Lorenzo Calibbi Junsong Cang Guofu Cao Jianshe Cao Antoine Chance Xuejun Chang yue Chang Zhe Chang Xinyuan Chang Wei Chao Auttakit Chatrabhuti Yimin Che yuzhi Che Bin Chen Danping Chen Fuqing Chen Fusan Chen Gang Chen Guoming Chen Hua-Xing Chen Huirun Chen Jinhui Chen Ji-yuan Chen Kai Chen Mali Chen Mingjun Chen Mingshui Chen Ning Chen Shanhong Chen Shanzhen Chen Shao-Long Chen Shaomin Chen Shiqiang Chen Tianlu Chen Wei Chen Xiang Chen Xiaoyu Chen Xin Chen Xun Chen Xurong Chen Ye Chen Ying Chen yukai Chen Zelin Chen Zilin Chen Gang Chen Boping Chen Chunhui Chen Hok Chuen Cheng Huajie Cheng Shan Cheng Tongguang Cheng yunlong Chi Pietro Chimenti Wen Han Chiu Guk Cho Ming-Chung Chu Xiaotong Chu Ziliang Chu Guglielmo Coloretti Andreas Crivellin Hanhua Cui Xiaohao Cui Zhaoyuan Cui Brunella D'Anzi Ling-yun Dai Xinchen Dai Xuwen Dai Antonio De Maria Nicola De Filippis Christophe De La Taille Francesca De Mori Chiara De Sio Elisa Del Core Shuangxue Deng Wei-Tian Deng Zhi Deng Ziyan Deng Bhupal Dev {4. Dewen Biagio Di Micco Ran Ding Siqin Dingl Yadong Ding Haiyi Dong Jianin AGH university of Science and Technology Faculty of Physics and Applied Computer ScienceKrakow Anhui university HefeiAnhui Applied Physics Department Federal Urdu University of ArtsScience and TechnologyIslamabad Banaras Hindu university Institute of ScienceVaranasi Bandirma Onyedi Eylul university Bandirma Beihang university Beijing Beijing Computational Science Research center Beijing Beijing Conveyi LTD Beijing Beijing Glass Research Institute Co. LtdBeijing Beijing Institute of Technology Beijing Beijing Normal university Beijing Brown university ProvidenceRI Budker Institute of Nuclear Physics Novosibirsk Cadi Ayyad university Laboratory of Fundamental and Applied PhysicsPolydisciplinary FacultyMarrakech Cairo university Giza center for Theory and Computation National Tsing Hua UniversityHsinchu Central china Normal university WuhanHubei Central South university ChangshaHunan Centro de Fisica Teorica de Particulas Universidade Tecnica de LisboaLisboa Chengdu KaitengSifang Digital Radio&TV Equipment Co. LtdChengduSichuan china academy of engineering physics MianyangSichuan china building materials academy Beijing china center of Advanced Science and Technology Beijing china Institute of Atomic Energy Beijing china Jiliang university HangzhouZhejiang china Nuclear Instrument CO. LTDBeijing china university of Geosciences Beijing china university of Geosciences WuhanHubei china university of Mining and Technology XuzhouJiangsu china West Normal university NanchongSichuan Chongqing university Department of PhysicsChongqing Chulalongkorn university Bangkok City university of Hong Kong Department of PhysicsHong Kong Cockcroft Institute Daresbury Cornell university IthacaNY CPPM(AMU/IPhU&CNRS/INP) Marseille Dalian Minzu university DalianLiaoning Dalian university of Technology Department of PhysicsDalian Deutsches Elektronen-Synchrotron DESY Hamburg Dzhelepov Laboratory of Nuclear Problems of Joint Institute for Nuclear Research(DLNP JINR) Dubna East china university Of Science And Technol

The Circular Electron Positron Collider(CEPC)is a large scientific project initiated and hosted by china,fostered through extensive collaboration with international *** complex comprises four accelerators:a 30 GeV Linac,a 1.1 GeV Damping Ring,a Booster capable of achieving energies up to 180 GeV,and a Collider operating at varying energy modes(Z,W,H,and tt).The Linac and Damping Ring are situated on the surface,while the subterranean Booster and Collider are housed in a 100 km circumference underground tunnel,strategically accommodating future expansion with provisions for a potential Super Proton Proton Collider(SPPC).The CEPC primarily serves as a Higgs *** its baseline design with synchrotron radiation(SR)power of 30 MW per beam,it can achieve a luminosity of 5×10^(34.cm^(-2)s^(-1)per interaction point(IP),resulting in an integrated luminosity of 13 ab^(-1)for two IPs over a decade,producing 2.6 million Higgs *** the SR power to 50 MW per beam expands the CEPC's capability to generate 4.3 million Higgs bosons,facilitating precise measurements of Higgs coupling at sub-percent levels,exceeding the precision expected from the HL-LHC by an order of *** Technical Design Report(TDR)follows the Preliminary Conceptual Design Report(Pre-CDR,2015)and the Conceptual Design Report(CDR,2018),comprehensively detailing the machine's layout,performance metrics,physical design and analysis,technical systems design,R&D and prototyping efforts,and associated civil engineering ***,it includes a cost estimate and a preliminary construction timeline,establishing a framework for forthcoming engineering design phase and site selection *** is anticipated to begin around 2027-2028,pending government approval,with an estimated duration of 8 *** commencement of experiments and data collection could potentially be initiated in the mid-2030s.

关键词： initiated exceeding precise

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