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Discovery of immune related factors in Fenneropenaeus chinensis by annotation of ESTs

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Progress in Natural Science:Materials International 2004年第1期14卷 48-55页

作者： SHEN Yaoqing 1,2 , XIANG Jianhai 1*, WANG Bing 1,2 , LI Fuhua 1 and TONG Wei 3.1. Experimental Marine Biology Laboratory, institute of Oceanology, chinese academy of Sciences, Qingdao 266071,{3. 2. Graduate School of the chinese academy of Sciences, beijing 10003., china 3. genomics & Bioinformatics institute, chinese academy of Sciences, beijing 1013.0, china) chinese Acad Sci Inst Oceanol Expt Marine Biol Lab Qingdao 266071 Peoples R China chinese Acad Sci Grad Sch Beijing 100039 Peoples R China chinese Acad Sci Geonom & Bioinformat Inst Beijing 101300 Peoples R China

A total of 10446 expressed sequence tags (ESTs) are obtained by a large scale sequencing of a cDNA library from cephalothorax of adult Fenneropenaeus chinensis . An EST analysis platform was built up based on local computers and bioinformatic techniques were used to annotate these ESTs in order to promptly find possible functional genes, especially for immune related factors. About 4% of the ESTs show similarity to the coding sequences of such factors, including lectin, serine protease, serpin, lysozyme, etc. These ESTs provide a partial profile of the immune system in F. chinensis and useful information for further study on these genes.

关键词： Fenneropenaeus chinensis bioinformatics ESTs immune factors

High Throughput SNP Genotyping with Two Mini-sequencing Assays

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Acta Biochimica et Biophysica Sinica 2004年第6期36卷 379-384页

作者： Chunqing LUO1,2#, Libin DENG1#, and Changqing ZENG1,2* The institute of Genetic and Developmental Biology, the chinese academy of Sciences, {3. 100101, china 2{3. genomics institute, the chinese academy of Sciences, {3. 1013.0, china The institute of Genetic and Developmental Biology the Chinese Academy of SciencesBeijing 100101 China beijing genomics institute the Chinese Academy of SciencesBeijing 101300 China

Two mini-sequencing methods, FP-TDI (template-directed dye-terminator incorporation with fluorescence-polarization) and MassArray (matrix assisted laser desorption ionization time of flight detection mass spectrometry), were optimized. A numeric standard was introduced to evaluate the SNP scoring quality of FP-TDI assay, thus made the optimization work easier. At the same time, using multi-PCR technology, 8-plex genotyping of MassArray assay was successfully carried out, some softwares were developed and the data process of MassArray was highly automated. Then these two methods were applied to high throughput SNP genotyping, the accuracy, efficiency and robustness were compared. The result shows FP-TDI is more sensitive to the concentration of SNPprimer and PCR product, as well as extension cycles, the SNPprimer length of FP-TDI should be 24–3. bp long, whereas MassArray assay prefers to be as short as only 16 *** 6440 SNP sites of human chromosome 3.were genotyped in a sample of 90 individuals, 4792 sites by FP-TDI assay and 1648 sites by MassArray assay, the success rates of FP-TDI and MassArray were 67.7% and 93.6% respectively. The throughput of MassArray was higher than FP-TDI, and the cost of MassArray was lower, MassArray was more suitable for high throughput SNP genotyping.

关键词： SNP genotyping FP-TDI MassArray high throughput

^(40)Ar/^(3.)Ar dating of shear deformation of the Xianshuihe fault zone in west Sichuan and its tectonic significance

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Science china Earth Sciences 2004年第9期47卷 794-803页

作者： ZHANG Yueqiao1, CHEN Wen2, 3.& YANG Nong1 1. institute of Geomechanics, chinese academy of Geological Sciences, {3. 100081, china 2. institute of Geology, chinese academy of Geological Sciences, {3. 10003., china 3. institute of Geology and Geophysics, chinese academy of Sciences,{13., china institute of Geomechanics Chinese Academy of Geological Sciences Beijing 100081 China institute of Geology Chinese Academy of Geological Sciences Beijing 100037 China institute of Geology and Geophysics Chinese Academy of Sciences Beijing 100029 China

Based on field geological survey, structural measurements and classical Ar/Ar dating of mica, biotite and K-feldspar, we obtain cooling ages for Miocene left-lateral shear along the Xianshuihe fault zone. The results document two thermal events during the sinistral shear. The early event (12-10 Ma) corresponds to rapid cooling of the Zheduoshan granitic massif from above 700℃ to below 3.0℃. The late event (5-3.5 Ma) corresponds to cooling of granites intruded along the eastern side of the fault zone. These dating results provide important thermochronological constraint on the timing of late Cenozoic eastward extrusion of the Chuan-Dian Block in the SE Tibetan margin.

关键词： Xianshuihe fault zone, Zheduoshan granitic massif, sinistral shear deformation, 40Ar/3.Ar dating, late Cenozoic.

Strong decays of N~*(153.) in an extended chiral quark model

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Science china(Physics,Mechanics & Astronomy) 2009年第10期52卷 1452-1457页

作者： AN ChunSheng1,2,3.& ZOU BingSong1,2,3.1 institute of High Energy Physics,chinese academy of Sciences,{3. 100049,china 2 Theoretical Physics Center for Science Facilities,chinese academy of Sciences,{3. 100049,china 3.Graduate University of chinese academy of Sciences,{3. 100049,china institute of High Energy Physics Chinese Academy of Sciences Beijing 100049 China Theoretical Physics Center for Science Facilities Chinese Academy of Sciences Beijing 100049 China Graduate University of chinese academy of Sciences Beijing 100049 China

The strong decays of the N*(153.) resonance are investigated in an extended chiral quark model by including the low-lying $ qqqq\bar q $ components in addition to the qqq component. The results show that these five-quark components in N*(153.) contribute significantly to the N*(153.) → Nπ and N*(153.) → Nη decays. The contributions to the Nη decay come from both the lowest energy and the next-tolowest energy five-quarks components, while the contributions to the Nπ decay come from only the latter one. Taking these contributions into account, the description for the strong decays of N*(153.) is improved, especially for the puzzling large ratio of the decays to Nη and Nπ.

关键词： N*(153.) strong decays five-quark components

A mixed method for measuring low-frequency acoustic properties of macromolecular materials

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Science china(Physics,Mechanics & Astronomy) 2006年第6期49卷 729-739页

作者： LIU Hongwei1,YAO Lei1,3.ZHAO Hong1,3.ZHANG Jichuan2,3.& XUE Zhaohong2 1. National Key Laboratory of Acoustics,institute of Acoustics,chinese academy of Sciences,{3. 100080,china 2. institute of Chemistry,chinese academy of Sciences,{3. 100080,china 3. Graduate University of chinese academy of Sciences,{3. 100049,china National Key Laboratory of Acoustics Institute of Acoustics Chinese Academy of Sciences Beijing 100080 China institute of Chemistry Chinese Academy of Sciences Beijing 100080 China Graduate University of chinese academy of Sciences Beijing 100049 China

A mixed method for measuring low-frequency acoustic properties of macro-molecular materials is presented. The dynamic mechanical parameters of materials are first measured by using Dynamic Mechanical Thermal Apparatus(DMTA) at low frequen-cies,usually less than 100 Hz; then based on the Principles of Time-Temperature Super-position (TTS),these parameters are extended to the frequency range that acousticians are concerned about,usually from hundreds to thousands of hertz; finally the extended dynamic mechanical parameters are transformed into acoustic parameters with the help of acoustic measurement and inverse analysis. To test the feasibility and accuracy,we measure a kind of rubber sample in DMTA and acquire the basic acoustic parameters of the sample by using this method. While applying the basic parameters to calculating characteristics of the sample in acoustic pipe,a reasonable agreement of sound absorp-tion coefficients is obtained between the calculations and measurements in the acoustic pipe.

关键词： low-frequency acoustic properties,dynamic mechanical properties,Principles of Time-Temperature Superposition,acoustic pipe,sound absorption coefficient.

Application of asymptotic waveform approximation technique to hybrid FE/BI method for 3. scattering

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Science in china(Series F) 2007年第1期50卷 124-134页

作者： PENG Zhen1,2,3.& SHENG XinQing2 1 institute of Electronics, chinese academy of Sciences, {3. 100080, china 2 Department of Electronic Engineering, {3. institute of Technology, {3. 100081, china 3.Graduated University of chinese academy of Sciences, {3. 10003., china 1. institute of Electronics Chinese Academy of Sciences Beijing 100080 China 2. Department of Electronic Engineering Beijing Institute of Technology Beijing 100081 China 3. Graduated University of chinese academy of Sciences Beijing 100039 China

The asymptotic waveform evaluation （AWE） technique is a rational function approximation method in computational mathemetics, which is used in many appllcations in computational electromagnetlca. In this paper, the performance of the AWE technique in conjunction with hybrid finite element/boundary integral （FE/BI） method is firstly Inveetlgeted. The formuletlon of the AWE applied in hybrid FE/BI method is given in detail. The characteristic implementation of the application of the AWE to the hybrid FE/BI method is discussed. Numarlcel results demonstrate that the AWE technique can greatly speed up the hybrid FE/BI method to acquire wide-band and wide-angle backecatter radar-cross-section （RCS） by complex targets.

关键词： hybrid FE/BI AWE electromagnetic scattering RCS

SHRIMP zircon U-Pb geochronology of early Mesozoic felsic igneous rocks from the southern Lancangjiang and its tectonic implications

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Science china Earth Sciences 2006年第10期49卷 1032-1042页

作者： PENG Touping1,2, WANG Yuejun1, FAN Weiming1, LIU Dunyi3. SHI Yuruo3.& MIAO Laicheng4 1. Key Laboratory of Isotope Geochronology and Geochemistry, Guangzhou institute of Geochemistry, chinese academy of Sciences, Guangzhou 510640,{3. 2. Graduate University of chinese academy of Sciences, beijing 10003.,{3. 3. SHRIMP isotope Laboratory, chinese academy of Geological Sciences, beijing 10003.,{3. 4. institute of Geology and Geophysics, chinese academy of Sciences, beijing 10002.,{3. Key Laboratory of Isotope Geochronology and Geochemistry Guangzhou Institute of Geochemistry Chinese Academy of Sciences Guangzhou China Graduate University of chinese academy of Sciences Beijing China SHRIMP isotope Laboratory Chinese Academy of Geological Sciences Beijing China institute of Geology and Geophysics Chinese Academy of Sciences Beijing China

The SHRIMP zircon U-Pb geochronology of three typical samples, including two monzo nitic granites from the Lincang batholith and a rhyolite from the Manghuai Formation are presented in the southern Lancangjiang, western Yunnan Province. The analyses of zircons for the biotite monzonitic granites from the northern (02DX-13.) and southern (20JH-10) Lincang batholith show the single and tight clusters on the concordia, and yield the weighted mean 206Pb/23.U ages of 229.4 ± 3.0 Ma and 23..4 ± 3.6 Ma, respectively, representing the crystallized ages of these granites. The zircons for the rhyolitic sample (02DX-95) from the Manghuai Formation give a weighted mean 206Pb/23.U age of 23..0 ± 5.0 Ma. These data suggest that the igneous rocks from the Lincang granitic batholith and Manghuai Formation have a similar crystallized age. In combination with other data, it is inferred that both were generated at a narrow age span (~23. Ma) and were originated from the postcollisional tectonic regime. An early Proterozoic 206Pb/23.U apparent age of 1977±44 Ma is additionally obtained from one zircon from the biotite monzonitic granite (southern Lincang batholith), indicative of devel- opment of the early Proterozoic Yangtze basement in the region. These precisely geochronological data provide important constraints on better understanding the Paleozoic tectonic evolution of the Tethys, western Yunnan Province.

关键词： SHRIMP zircon U-Pb dating, Middle Triassic, Lincang granitic batholith, Manghuai Formation rhyolite, southern Lancangjiang.

Modeling and verifying Web services driven by requirements:An ontology-based approach

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Science in china(Series F) 2006年第6期49卷 792-820页

作者： HOU Lishan1,3.JIN ZHi1,2 & WU Budan1,4 1. academy of Mathematics and Systems Science, chinese academy of Sciences, {3. 100080, china 2. institute of Computing Technology, chinese academy of Sciences, {3. 100080, china 3. institute of Software, chinese academy of Sciences, {3. 100080, china 4. Graduate University of chinese academy of Sciences, {3. 100049, china 1. academy of Mathematics and Systems Science Chinese Academy of Sciences Beijing 100080 China 3. institute of Software Chinese Academy of Sciences Beijing 100080 China 2. institute of Computing Technology Chinese Academy of Sciences Bejing 100080 China 4. Graduate University of chinese academy of Sciences Beijing 100049 China

Automatic discovery and composition of Web services is an important research area in Web service technology, in which the specification of Web services is a key issue. This paper presents a Web service capability description framework based on the environment ontology. This framework depicts Web services capability in two aspects： the operable environment and the environment changes resulting from behaviors of the Web service. On the basis of the framework, a requirement-driven Web service composition model has been constructed. This paper brings forward the formalization of Web service interactions with π calculus. And an automatic mechanism converting conceptual capability description to the formal process expression has been built. This kind of formal specification assists in verifying whether the composite Web service model matches the requirement.

关键词： Web service environment ontology automatic composition π calculus formal verification.

A new DNA algorithm to solve graph coloring problem

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Progress in Natural Science:Materials International 2007年第6期17卷 733-738页

作者： Jiang Xingpeng1, Li Yin2, Meng Ya3.and Meng Dazhi2(1. National Laboratory of Pattern Recognition,institute of Automation, chinese academy of Sciences, {3. 100080, china 2. College of Applied Sciences, {3. University of Technology, beijing 10002., china 3. School of Science, {3. institute of Technology, {3. 100081, china) National Laboratory of Pattern Recognition Institute of Automation Chinese Academy of Sciences Beijing 100080 China College of Applied Sciences Beijing University of Technology Beijing 100022 China School of Science Beijing Institute of Technology Beijing 100081 China

Using a small quantity of DNA molecules and little experimental time to solve complex problems successfully is a goal of DNA computing. Some NP-hard problems have been solved by DNA computing with lower time complexity than conventional computing. However, this advantage often brings higher space complexity and needs a large number of DNA encoding molecules. One example is graph coloring problem. Current DNA algorithms need exponentially increasing DNA encoding strands with the growing of problem size. Here we propose a new DNA algorithm of graph coloring problem based on the proof of four-color theorem. This algorithm has good properties of needing a relatively small number of operations in polynomial time and needing a small number of DNA encoding molecules (we need only 6R DNA encoding molecules if the number of regions in a graph is R).

关键词： DNA computing NP-hard problem graph coloring problem.