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Effects of adenoviral gene transfer of mutated IκBα,a novel inhibitor of NF-κB,on human monocyte-derived dendritic cells

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Acta Pharmacologica Sinica 2006年第5期27卷 609-616页

作者： Lin-fu ZHOU~(2.5) Ming-shun ZHANG~3.Kai-sheng YIN~(2.5) Yong JI~4 Wei-ping XIE~2.Xue-fan CUI~2.Xiao-hui JI~3.~2.epartment of Respiratory Medicine,the First Affiliated Hospital.~3.epartment of Microbiology and Immunology.~4Atheroscleorosis research center,{2. Medical University,{2. 2.002.,china Department of Respiratory Medicine the First Affiliated Hospital Nanjing Medical University Nanjing China Department of Microbiology and Immunology Nanjing Medical University Nanjing China Atheroscleorosis research center Nanjing Medical University Nanjing China

Aim:To investigate the effects of adenoviral gene transfer of IκBα mutant (IκBαM),a novel inhibitor of nuclear factor κB(NF-κB),on apoptosis,phenotype and func-tion of human monocyte-derived dendritic cells (DC).Methods:Monocytes,cocultured with granulocyte/macrophage colony-stimulating factor (GM-CSF;900ng/mL) and interleukin (IL)-4 (3.0ng/mL) for 5d,followed by stimulation withlipopolysaccharide (LPS;100ng/mL) for 2. differentiated into mature ***-cytes were either left untransfected or were transfected with AdIκBαM or *** transcription and expression of the IκBαM gene,and the inhibitory effect ofIκBαM on tumor necrosis factor (TNF)-α-induced NF-κB activation in mature DCwere detected by polymerase chain reaction (PCR),reverse transcription-poly-merase chain reaction (RT-PCR),Western blot analysis,and electrophoretic mobil-ity shift assays,*** phenotype,apoptosis,IL-12.secretion level ofDC,and ability to stimulate the proliferation of T cells were determined by flowcytometry,enzyme-linked immunosorbent assay and mixed leukocyte ***:PCR and RT-PCR were used to detect a unique 801 bp band in AdIκBαM-transfected mature DC,and also a dose-and time-dependent expression of theIκBαM gene,which peaked at a multiplicity of infection of 100pfu/cell and at ***,AdIκBαM significantly suppressed the TNF-α-induced NF-κBactivation,augmented apoptosis,downregulated CD80,CD83.and CD86 surfacemolecules,IL-12.secretion levels and the ability to stimulate the proliferation of Tcells in mature ***:AdIκBαM effectively transfected and potentlyinhibited NF-κB activation in monocyte-derived mature *** ofthe IκBαM gene in mature DC may contribute to T-cell immunosuppression throughinduction of DC apoptosis and downregulation of B7 molecules,providing a po-tential strategy for future DC-based immunotherapy of asthma.

关键词： dendritic cells nuclear factor κB IκBαmutant immunotherapy asthma

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Evaluation of a Novel Bioabsorbable PRGD/PDLLA/β-TCP/NGF Composites in Repair of Peripheral Nerves

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Journal of Wuhan University of Technology(Materials Science) 2009年第3期24卷 409-414页

作者：王永红李世普 Hospital Wuhan University of Technology Biomedical Materials and Engineering research center Wuhan University of Technology

Peripheral nerve regeneration using a novel nerve conduit （PRGD/PDLLA/β TCP/NGF） was evaluated, which was made of RGD peptide modified poly{（lactic acid）-co-[（glycolic acid）-alt-（L-lysine）]} （PRGD）, poly（d,l-lactic acid）（PDLLA） and β-tricalcium phosphate （β-TCP）. And the effectiveness was compared with that of PRGD/PDLLA/β-TCP, PDLLA and autograft in terms of nerve regeneration across a gap. Both of biodegradablity and cell-biocompatibility of the novel nerve conduit were evaluated in vitro. The results show that PRGD/PDLLA/β-TCP/NGF composite ma terials have better biodegradation properties and cell affinity than PDLLA, and could promote the RSC96 Schwann cells adhesion, proliferation and growth on the surface of materials. PRGD/PDLLA/ β-TCP/NGF composite conduit was significantly superior to that of the PDLLA conduit in histological and axon morphologic index. PRGD/PDLLA/β-TCP/NGF conduit is more beneficial to nerve regeneration than PDLLA conduit. The biodegradable PDLLA/PRGD/β-TCP/NGF conduit has a good biocompatibility with rats tissue and it could effectively promote the nerve regeneration after bridging sciatic nerve defect of rats, the effect is as good as that of the autograft nerve, significantly superior to the PRGD/PDLLA/β-TCP conduit and PDLLA conduit. PDLLA/PRGD/β-TCP/NGF composite conduit is a potential ideal conduit.

关键词： peripheral nerve nerve conduit PDLLA/PRGD/β-TCP/NGF composite regeneration

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Adsorption of zinc on manganite(γ-MnOOH): particle concentration effect and adsorption reversibility

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Journal of Environmental Sciences 2004年第4期16卷 627-630页

作者： QIN Yan-wen1, PAN Gang 1,2., ZHANG Ming-ming1, LI Xian-liang1 (1. State Key Laboratory of Environmental Aquatic Chemistry, research center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, {2.. 2. Qingdao University of Science & Technology, Qingdao 2.6042. {2.) State Key Laboratory of Environmental Aquatic Chemistry Research Center for Eco-Environmental Sciences Chinese Academy of Sciences Beijing 100085 China.

The adsorption and desorption processes of Zn(Ⅱ) on γ-MnOOH as a function of particle concentrations(C p) were studied. An obvious C p effect was observed in this adsorption system. The degree of adsorption hysteresis increased greatly with the increasing of C p, indicating that the extent of the real metastable-equilibrium states deviating from the ideal equilibrium state was enhanced with the increasing of C p. The C p-reversibility relationship confirmed the metastable-equilibrium adsorption(MEA) inequality(Pan, 1998a), which was the core formulation of the MEA theory. Because the MEA inequality was based on the basic hypothesis of MEA theory that adsorption density Γ is not a state variable, the C p-reversibility relationship gave indirect evidence to the basic hypothesis of MEA theory.

关键词： zinc γ-MnOOH adsorption reversibility

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The biomarkers of 2.6,10,15, 19-pentamethylicosenes and their carbon isotopic composition in the sediments from the Gulf of Mexico

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Chinese Science Bulletin 2006年第14期51卷 1736-1740页

作者： SONG Zhiguang1,2.& WANG Cuiping1,3.. State Key Laboratory of Organic Geochemistry, Guangzhou Institute of Geochemistry, Chinese Academy of Sciences, Guangzhou 510640, china 2. Guangzhou {3. {2. of Gas Hydrate, Chinese Academy of Sciences, Guangzhou 510640, china 3. Graduate School of Chinese Academy of Sciences, Beijing 10003., china State Key Laboratory of Organic Geochemistry Guangzhou Institute of Geochemistry Chinese Academy of Sciences Guangzhou China Guangzhou research center of Gas Hydrate Chinese Academy of Sciences Guangzhou China Graduate School of Chinese Academy of Sciences Beijing China

A group of 2.6,10,15,19-pentamethyli- cosenes (PMIΔ) containing 1―5 unsaturated double bonds has been identified in the sea floor sediments from the Gulf of Mexico at the Green Canyon 2.8 *** PMIΔ compounds are distributed between nC2. and nC2. on the mass chromatogram of aliphatic fraction. Their δ 13. values are very much depleted in 13. and in the range of ?86.7‰ to ?115.5‰, whereas the δ 13. values of companion n-alkanes range from ?2..4‰ to ?3..6‰. These unsaturated PMIΔ com-pounds are typical biomarkers derived from the an-aerobic oxidation of methane mediated by meth-ane-oxidizing archaeal bacteria and indicative of the gas seeps or even the occurrence of gas hydrates in the deep sea sediments.

关键词：生物标志墨西哥湾沉积物 PMIA 天然气水化物

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A new algorithm for microwave radiometer remote sensing of sea surface salinity and temperature

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Science china Earth Sciences 2006年第11期49卷 1204-1211页

作者： YIN Xiaobin1, LIU Yuguang1, WANG Zhenzhan2.& XIU Peng1 1. Physical Oceanography Laboratory, Ocean University of china, Qingdao 2.6003. china 2. {2. for Space and Applied research, Chinese Academy of Sciences, Beijing 100080, china Physical Oceanography Laboratory Ocean University of China Qingdao China center for Space and Applied research Chinese Academy of Sciences Beijing China

The microwave radiation of the sea surface, which is denoted by the sea surface brightness temperature, is not only related with sea surface salinity (SSS) and temperature (SST), but also influenced by sea surface wind. The errors of wind detected by satellite sensor have significant influences on the accuracy of SSS and SST retrieval. The effects of sea surface wind on sea surface brightness temperature, i.e. △Th,v, and the relations among △Th,v, wind speed, sea surface tempera- ture, sea surface salinity and incidence angle of observation are investigated. Based on the investi- gations, a new algorithm depending on the design of a single radiometer with double polarizations and multi-incidence angles is proposed. The algorithm excludes the influence of sea surface wind on SSS and SST retrieval, and provides a new method for remote sensing of SSS and SST.

关键词： microwave radiometer, sea surface temperature, sea surface salinity, wind, brightness temperature.

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Synthesis of todorokite by refluxing process and its primary characteristics

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Science china Earth Sciences 2004年第8期47卷 760-768页

作者： FENG Xionghan1, LIU Fan1, TAN Wenfeng1, LIU Xiangwen2.& HU Hongqing1 1. College of resources and Environment, Huazhong Agricultural University, Wuhan 43.070, china 2. Testing {2., china University of Geosciences, Wuhan 43.074, china College of resources and Environment Huazhong Agricultural University Wuhan China Testing center China University of Geosciences Wuhan China

Single phase and well-crystallined todorokite were synthesized by heating and refluxing process from birnessite as a precursor. The average chemical composition of the synthesized todorokites by refluxing for 8 h and for 2. h was Mg0.19MnO2.11(H2.)1.15 and in0.17-MnO2.10(H2.)0.88, respectively. The crystallinity of the todorokite increased and no other phase was produced with increasing refluxing period. The synthesized todorokites have the same morphologies and the similar structural characteristics with the natural todorokites and hydro-thermally synthesized samples. The chemical compositions of the synthetic tordorokites by refluxing process are close to those of todorokites synthesized by hydrothermal process, except a higher average oxidation state of Mn for the former.

关键词： todorokite,birnessite,manganese oxide,refluxing,hydrothermal,synthesis.

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A tentative study for the prediction of the CME related geomagnetic storm intensity and its transit time

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Science china(Technological Sciences) 2005年第6期48卷 648-668页

作者： ZHAO Xinhua1,2.& FENG Xueshang1,3.1. SIGMA Weather Group, Key Laboratory of Space Weather, center for Space Science and Applied Re-search, Chinese Academy of Sciences, Beijing 100080, china 2. Graduate School of the Chinese Academy of Sciences, Beijing 10003., china 3. School of Geophysics and Geoinformation Systems, china University of Geosciences, Beijing 100083. china SIGMA Weather Group Key Laboratory of Space Weather Center for Space Science and Applied Research Chinese Academy of Sciences Beijing China Graduate School of the Chinese Academy of Sciences Beijing China School of Geophysics and Geoinformation Systems China University of Geosciences Beijing China

Using 80 CME-ICME events during 1997.1―2.02.9, based on the eruptive source locations of CMEs and solar magnetic field observation at the photosphere, a current sheet magnetic coordinate (CMC) system is established in order to study the propagation of CME and its geoeffectiveness. In context of this coordinate system, the effect of the eruptive source location and the form of heliospheric current sheet (HCS) at the eruptive time of CME on the geomagnetic storm intensity caused by CME and the CME’s transit time at the Earth is investigated in detail. Our preliminary conclusions are: 1) The geomagnetic disturbances caused by CMEs tend to have the so-called “same side-opposite side effect”, i.e. CMEs erupt from the same side of the HCS as the earth would be more likely to arrive at the earth and the geomagnetic disturbances associated with them tend to be of larger magnitude, while CMEs erupting from the opposite side would arrive at the earth with less probability and the corresponding geomagnetic disturbance magnitudes would be relatively weaker. 2. The angular separation between the earth and the HCS affect the corresponding disturbance intensity. That is, when our earth is located near the HCS, adverse space weather events occur most probably. 3. The erupting location of the CME and its nearby form of HCS will also affect its arrival time at the earth. According to these conclusions, in this context of CMC coordinate we arrive at new prediction method for estimating the geomagnetic storm intensity (Dstmin) caused by CMEs and their transit times. The application of the empirical model for 80 CME-ICME events shows that the relative error of Dst is within 3.% for 59% events with Dstmin≤?50 nT, while the averaged absolute error of transit time is lower than 10 h for all events.

关键词： coronal mass ejections, current sheet magnetic coordinate system, geomagnetic storm intensity, transit time, prediction method.

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Numerical simulation of topography effects on the “00.7” severe rainfall in Beijing

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Progress in Natural Science:Materials International 2005年第9期15卷 818-826页

作者： ZHANG Chaolin1**, JI Chongping1, KUO Yinghwa2. FAN Shuiyong1, XUAN Chunyi3.and CHEN Min1(1. Institute of Urban Meteorology, china Meteorological Administration, Beijing 100089, china 2. Mesoscale and Microscale Meteorology Division, N{2. center for Atmospheric research, Boulder, CO 803.7-3.00, USA 3. Beijing Meteorological Bureau, Beijing 100089, china) Institute of Urban Meteorology China Meteorological Administration Beijing 100089 China Mesoscale and Microscale Meteorology Division National Center for Atmospheric Research Boulder CO 80307-3000 United States Beijing Meteorological Bureau Beijing 100089 China

In an effort to study the severe rainfall event of 4—5th July 2.00 in Beijing (with 2.h accumulated precipitation of 2.0 mm), we perform numerical simulations to investigate this event using the MM5v3.6 model. The model is initialized with the MM5/WRF 3.VAR analysis, which incorporates the ground-based GPS precipitable water vapor, automatic and conventional meteorological observations in its assimilation step. For 2. h accumulated precipitation forecast, the threat scores are 0.72. 0.76, 0.67 and 0.63. for thresholds of 1, 5, 10, and 2. mm, respectively. Holding other factors unchanged, sensitivity experiments were conducted with different topographic resolutions of 110, 50 and 3.7 km to investigate the topographic effects on precipitation over the Beijing area. In these sensitivity experiments, we attempt to preserve the realistic orographic distribution in the model topography under the condition of keeping the dynamic-thermodynamic consistency of the initial model atmosphere to the extent possible. Results indicate that the unique topographic distribution and variations in the Beijing area play an important role in determining the location, distribution and intensity of heavy precipitation.

关键词： topography heavy rainfall 3.VAR ground-based GPS numerical simulation.

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Nonlinear interactions between gravity waves and background winds

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Progress in Natural Science:Materials International 2007年第6期17卷 639-644页

作者： Liu Xiao1,2.and Xu Jiyao1(1. State Key Laboratory for Space Weather, center for Space Science and Applied research, Chinese Academy of Sciences, Beijing 100080, {2. 2. Graduate University of Chinese Academy of Sciences, Beijing 10003., {2.) State Key Laboratory for Space Weather Chinese Academy of Sciences Beijing 100080 China center for Space Science and Applied research Chinese Academy of Sciences Beijing 100080 China Graduate University Chinese Academy of Sciences Beijing 100039 China

Using the nonlinear propagating gravity waves (GW) model in the two-dimensional compressible atmosphere and the linear GW theory, the process of GW propagation in different background winds, e.g. the direction of the background wind is opposite to (dead wind) or the same as (tail wind) the direction of the horizontal phase velocity of GW, is studied. The results show that the dead wind prolongs the vertical wavelength and accelerates GW propagation. Therefore, GW propagates up to a higher height becomes instable in a short time and eventually induces an inverse jet flow. Then, the vertical wavelength is becoming short due to the nonlinear interactions between GW and the inverse jet flow. The vertical wavelength and group velocity decrease after GW propagates into the tail wind. The initial instable time is delayed. Although most of GW is trapped in the instable region, some of GW propagates above the instable region. Compared with GW propagation in the tail wind, the nonlinear interactions between GW and the dead wind are also strong. In contrast, the linear GW theory predicts that GW can propagate freely in the dead wind. The vertical wavelength simulated by the nonlinear numerical model is different from that predicted by the linear theory greatly after GW propagates into the dead wind.

关键词： gravity waves background winds vertical group velocity vertical wavelength nonlinear interactions.

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Molecular dynamics simulations of interaction between protein-tyrosine phosphatase 1B and a bidentate inhibitor

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Acta Pharmacologica Sinica 2006年第1期27卷 100-110页

作者： Gui-xia LIU~(2.3.4) Jin-zhi TAN~2.Chun-ying NIU~2.Jian-hua SHEN~(2.3. Xiao-min LUO~2.Xu SHEN~(2.3. Kai-xian CHEN~2.Hua-liang JIANG~(2.3.4)2.center for Drug Discovery and Design,State Key Laboratory of Drug research,Shanghai Institute of Materia Medica,Shanghai Institutes for Biological Sciences,Chinese Academy of Sciences,Shanghai 2.12.3.{2. 3.School of Pharmacy,East {2. University of Science and Technology Shanghai 2.02.7,{2. center for Drug Discovery and Design State Key Laboratory of Drug Research Shanghai Institute of Materia Medica Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai China School of Pharmacy East China University of Science and Technology Shanghai China

Aim:To investigate the dynamic properties of protein-tyrosine phosphatase (PTP)1B and reveal the structural factors responsible for the high inhibitory potencyand selectivity of the inhibitor SNA for ***:We performed moleculardynamics (MD) simulations using a long time-scale for both PTP1B and PTP1Bcomplexed with the inhibitor SNA,the most potent and selective PTP1B inhibitorreported to *** trajectories were analyzed by using principal ***:Trajectory analyses showed that upon binding the ligand,theflexibility of the entire PTP1B molecule *** most notable change is themovement of the *** simulation results also indicated that electro-static interactions contribute more to PTP 1B-SNA complex conformation than thevan der Waals interactions,and that Lys41,Arg47,and Asp48 play important rolesin determining the conformation of the inhibitor SNA and in the potency andselectivity of the *** these,Arg47 contributed *** results werein agreement with previous experimental ***:The informationpresented here suggests that potent and selective PTP1B inhibitors can be de-signed by targeting the surface residues,for example the region containing Lys41,Arg47,and Asp48,instead of the second phosphate binding site (besides theactive phosphate binding site).

关键词： molecular dynamics simulation principal component analysis protein-tyrosine phosphatase 1B type 2.diabetes

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