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Risk of infection with arboviruses in a healthy population in Pakistan based on seroprevalence

Virologica Sinica 2024年第3期39卷 369-377页

作者： Shengyao Chen Muhammad Saqib Hafiz Sajid Khan yuan Bai Usman Ali Ashfaq Muhammad Khalid Mansoor Abulimti Moming Jing Liu Min zhou Saifullah Khan Niazi Qiaoli Wu Awais-Ur-Rahman Sial Shuang Tang Muhammad Hassan Sarfraz Aneela Javed Sumreen Hayat Mohsin Khurshid Iahtasham Khan Muhammad Ammar Athar Zeeshan Taj Bo Zhang Fei Deng Ali Zohaib Shu Shen Key Laboratory of Virology and National Virus Resource center Wuhan Institute of VirologyChinese Academy of SciencesWuhan 430071China university of Chinese Academy of Sciences Beijing 100049China Faculty of Veterinary Science University of Agriculture FaisalabadFaisalabad 38040Pakistan The Children's Hospital Faisalabad 38000Pakistan Department of Bioinformatics and Biotechnology Government College University FaisalabadFaisalabad 38000Pakistan f Department of Microbiology Faculty of Veterinary and Animal SciencesThe Islamia University of BahawalpurBahawalpur 63100Pakistan Hubei Jiangxia Laboratory Wuhan 430200China Key Laboratory of Special Pathogens and Biosafety Wuhan Institute of VirologyCenter for Biosafety Mega-ScienceChinese Academy of SciencesWuhan 430071China Armed Forces Institute of Pathology(AFIP) Rawalpindi 46000Pakistan Department of Clinical Studies Faculty of Veterinary&Animal SciencesPir Mehr Ali Shah Arid Agriculture UniversityRawalpindi 46300Pakistan Institute of Microbiology Government College University FaisalabadFaisalabad 38000Pakistan l Atta-ur-Rahman School of applied Biosciences National University of Science and TechnologyIslamabad 44000Pakistan Department of Clinical Sciences University of Veterinary and Animal SciencesLahore Sub-campusJhang 35200Pakistan Department of Molecular Pathology National Medical Center DHA Phase 1Karachi 75500Pakistan

Infectious diseases caused by arboviruses are a public health concern in ***,studies on data prevalence and threats posed by arboviruses are *** study investigated the seroprevalence of arboviruses in a healthy population in Pakistan,including severe fever with thrombocytopenia syndrome virus(SFTSV),Crimean-Congo hemorrhagic fever virus(CCHFV),Tamdy virus(TAMV),and Karshi virus(KSIV)based on a newly established luciferase immunoprecipitation system(LIPS)assays,and Zika virus(ZIKV)by enzyme-linked immunosorbent assays(ELISA).Neutralizing activities against these arboviruses were further examined from the antibody positive *** results showed that the seroprevalence of SFTSV,CCHFV,TAMV,KSIV,and ZIKV was 17.37%,7.58%,4.41%,1.10%,and 6.48%,respectively,and neutralizing to SFTSV(1.79%),CCHFV(2.62.),and ZIKV(0.69%)were identified,as well as to the SFTSV-related Guertu virus(GTV,0.83%).Risk factors associated with the incidence of exposure and levels of antibody response were ***,co-exposure to different arboviruses was demonstrated,as thirty-seven individuals were having antibodies against multiple viruses and thirteen showed neutralizing ***,individuals aged40 years,and outdoor workers had a high risk of exposure to *** these results reveal the substantial risks of infection with arboviruses in Pakistan,and indicate the threat from co-exposure to multiple *** findings raise the need for further epidemiologic investigation in expanded regions and populations and the necessity to improve health surveillance in Pakistan.

关键词： Arbovirus Pakistan Seroprevalence Tick-borne virus Mosquito-borne virus

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Oncogene goosecoid is transcriptionally regulated by E2.1 and correlates with disease progression in prostate cancer

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Chinese Medical Journal 2024年第15期137卷 1844-1856页

作者： Yue Ge Sheng Ma Qiang zhou Zezhong Xiong Yanan Wang Le Li Zheng Chao Junbiao Zhang Tengfei Li Zixi Wu yuan Gao Guanyu Qu Zirui Xi Bo Liu Xi Wu Zhihua Wang Department of Urology Tongji HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhanHubei 430030China Department of Urology Qinghai University Affiliated HospitalQinghai University Medical CollegeXiningQinghai 810001China Department of Oncology Tongji HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhanHubei 430030China Department of Urology First Hospital of Laohekou CityXiangyangHubei 441800China

Background:Although some well-established oncogenes are involved in cancer initiation and progression such as prostate cancer(PCa),the long tail of cancer genes remains to be ***(GSC)has been implicated in cancer ***,the comprehensive biological role of GSC in pan-cancer,specifically in PCa,remains *** aim of this study was to investigate the role of GSC in PCa ***:We performed a systematic bioinformatics exploration of GSC using datasets from The Cancer Genome Atlas,Genotype-Tissue Expression,Gene Expression Omnibus,German Cancer Research center,and our in-house ***,we evaluated the expression of GSC and its association with patient prognosis,and identified GSC-relevant genetic alterations in ***,we focused on the clinical characterization and prognostic analysis of GSC in *** understand the transcriptional regulation of GSC by E2. transcription factor 1(E2.1),we performed chromatin immunoprecipitation quantitative polymerase chain reaction(qPCR).Functional experiments were conducted to validate the effect of GSC on the tumor cellular phenotype and sensitivity to ***:GSC expression was elevated in various tumors and significantly correlated with patient *** alterations of GSC contribute to the progression of various tumors especially in *** with PCa and high GSC expression exhibited worse progression-free survival and biochemical recurrence ***,GSC upregulation in patients with PCa was mostly accompanied with higher Gleason score,advanced tumor stage,lymph node metastasis,and elevated prostate-specific antigen(PSA)***,the transcription factor,E2.1,stimulates GSC by binding to its promoter *** experiments further demonstrated that GSC acted as an oncogene and influenced the response of PCa cells to trametinib ***:GSC was highly overexpressed and strongly correlated with patient prognosis in

关键词： Goosecoid Prostate cancer Prognosis E2. transcription factor 1 Trametinib

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Natural and Socioeconomic Factors and Their Interactive Effects on House Collapse Caused by Typhoon Mangkhut

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International Journal of Disaster Risk Science 2021年第1期12卷 121-130页

作者： Xiangxue Zhang Juan Nie Changxiu Cheng Chengdong Xu Ling zhou Shi Shen yuan {2. Key Laboratory of Environmental Change and Natural Disaster Ministry of EducationBeijing Normal UniversityBeijing 100875China State Key Laboratory of Resources and Environmental Information System Institute of Geographic Sciences and Natural Resources ResearchChinese Academy of SciencesBeijing 100101China National Disaster Reduction center of china Ministry of Emergency ManagementBeijing 100124China State Key Laboratory of Earth Surface Processes and Resource Ecology Beijing Normal UniversityBeijing 100875China National Tibetan Plateau Data center Beijing 100101China

Typhoons are an environmental threat that mainly affects coastal regions *** interactive effects of natural and socioeconomic factors on the losses caused by typhoon disasters need further *** this study,GeoDetector was used to quantify the determinant powers of natural and socioeconomic factors and their interactive effects on the rate of house collapse in Guangdong and Guangxi Provinces of southeast china caused by Typhoon Mangkhut in *** further identify the dominant factors that influenced the disaster *** local indicators of spatial association method was then introduced to explain the spatial heterogeneity of the disaster losses under the influence of the dominant *** results indicate that both natural and socioeconomic factors significantly affected the house collapse *** maximum precipitation was the dominant factor,with a q value of 0.2.,followed by slope and elevation,with q values of 0.17 and 0.13,*** density and per capita gross domestic product had q values of 0.15 and0.13,*** all of the interactive effects of the influencing factors,the interactive effect of elevation and the ratio of brick-wood houses had the greatest influence(q=0.63)on the house collapse *** results can contribute to the formulation of more specific safety and property protection policies.

关键词： china Coastal regions GeoDetector House collapse rate Interactive effects Typhoon Mangkhut

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Large potential of strengthening the land carbon sink in china through anthropogenic interventions

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Science Bulletin 2024年第16期69卷 2622-2631页

作者： Xu Yue Hao zhou Yang Cao Hong Liao Xiaofei Lu Zhen Yu Wenping yuan Zhu Liu Yadong Lei Stephen Sitch Jürgen Knauer Huijun Wang Jiangsu Key Laboratory of Atmospheric Environment Monitoring and Pollution Control Jiangsu Collaborative Innovation Center of Atmospheric Environment and Equipment TechnologySchool of Environmental Science and EngineeringNanjing University of Information Science&Technology(NUIST)Nanjing 210044China College of Meteorology and Oceanography National University of Defense TechnologyChangsha 410073China Jiangsu Nanjing Environmental Monitoring center Nanjing 210013China Key Laboratory of Agrometeorology of Jiangsu Province Institute of EcologySchool of Applied MeteorologyNUISTNanjing 210044China Institute of Carbon Neutrality Sino-French Institute for Earth System ScienceCollege of Urban and Environmental SciencesPeking UniversityBeijing 100871China Department of Earth System Science Tsinghua UniversityBeijing 100084China State Key Laboratory of Severe Weather and Key Laboratory of Atmospheric Chemistry of CMA Chinese Academy of Meteorological SciencesBeijing 100081China College of Life and Environmental Sciences University of ExeterExeter EX44RJUK Hawkesbury Institute for the Environment Western Sydney UniversityPenrith 2751Australia Key Laboratory of Meteorological Disaster Ministry of EducationJoint International Research Laboratory of Climate and Environment ChangeCollaborative Innovation Center on Forecast and Evaluation of Meteorological DisastersNUISTNanjing 210044China

The terrestrial ecosystem in china mitigates 2.%-45%of the national contemporary fossil fuel CO_(2. emissions every *** and strengthening the land carbon sink is essential for reaching china’s target of carbon ***,this sink is subject to large uncertainties due to the joint impacts of climate change,air pollution,and human ***,we explore the potential of strengthening land carbon sink in china through anthropogenic interventions,including forestation,ozone reduction,and litter removal,taking advantage of a well-validated dynamic vegetation model and meteorological forcings from 16 climate *** anthropogenic interventions,considering Shared Socioeconomic Pathways(SSP)scenarios,the land sink is projected to be 0.2.-0.56 Pg C a^(-1) at 2.60,to which climate change contributes 0.06-0.13 Pg C a^(-1) and CO_(2. fertilization contributes 0.08-0.44 Pg C a^(-1) with the stronger effects for higher emission *** anthropogenic interventions,under a close-to-neutral emission scenario(SSP1-2.6),the land sink becomes 0.47-0.57 Pg C a^(-1) at 2.60,including the contributions of 0.12.Pg C a^(-1) by conservative forestation,0.07 Pg C a^(-1) by ozone pollution control,and 0.06-0.16 Pg C a^(-1) by 2.%litter removal over planted *** sink can mitigate 90%-110% of the residue anthropogenic carbon emissions in 2.60,providing a solid foundation for the carbon neutrality in china.

关键词： Carbon sink Forestation Ozone Litter removal

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Productive HBV infection of well-differentiated, hNTCP-expressing human hepatoma-derived(Huh7) cells

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Virologica Sinica 2017年第6期32卷 465-475页

作者： Ming zhou Kaitao Zhao Yongxuan Yao Yifei yuan Rongjuan {2. Yun Wang Jizheng Chen Xue Hu yuan zhou Xinwen Chen Chunchen Wu State Key Laboratory of Virology Wuhan Institute of Virology Chinese Academy of Sciences Shenzhen Xenotransplantation Research and Development center State and Local Joint Cancer Genome Clinical Application of Key Technology Laboratory Shenzhen Second People's Hospital First Affiliated Hospital of Shenzhen University Institute of Immunology Zhongshan School of Medicine Guangdong Provincial Key Laboratory of Organ Donation and Transplant Immunology Sun Yat-sen University

Feasible and effective cell models for hepatitis B virus(HBV) infection are required for investigating the complete lifecycle of this virus, including the early steps of viral entry. Resistance to dimethyl sulfoxide/polyethylene glycol(DMSO/PEG), h NTCP expression, and a differentiated state are the limiting factors for successful HBV infection models. In the present study, we used a hepatoma cell line(Hu7^(hDNTCPh)) to overcome these limiting factors so that it exhibits excellent susceptibility to HBV infection. To achieve this goal, different hepatoma cell lines were tested with 2.5% DMSO/4%PEG8000, and one resistant cell line(Huh7 D) was used to construct a stable h NTCP-expressing cell line(Hu7^(hDNTCPh)) using a recombinant lentivirus system. Then, the morphological characteristics and differentiation molecular markers of Hu7^(hDNTCPh) cells with or without DMSO treatment were characterized. Finally, the susceptibility of Hu7^(hDNTCPh) cells to HBV infection was assessed. Our results showed that Huh7 D cells were resistant to 2.5% DMSO/4% PEG8000, whereas the others were not. Hu7^(hDNTCPh) cells were established to express a high level of h NTCP compared to liver extracts, and Hu7^(hDNTCPh) cells rapidly transformed into a non-dividing, well-differentiated polarized phenotype under DMSO treatment. Hu7^(hDNTCPh) cells fully supported the entire lifecycle of HBV infection. This cell culture system will be useful for the analysis of host-virus interactions, which should facilitate the discovery of antiviral drugs and vaccines.

关键词： Hepatitis B virus(HBV) Na+/taurocholate cotransporting polypeptide(NTCP) Huh7 dimethyl sulfoxide(DMSO) polyethylene glycol(PEG) susceptibility

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钢铁生产主工序碳排放数字化仿真及流程改造

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铸造 2024年第6期73卷 825-834页

作者：侯明君王才加尚陈众计效园谢敬佩周建新华中科技大学材料成形与模具技术全国重点实验室湖北武汉430074 河南科技大学材料科学与工程学院河南洛阳471023 河南科技大学有色金属新材料与先进加工技术省部共建协同创新中心河南洛阳471023

对照我国提出的“双碳”战略,钢铁行业降碳减排时间紧,任务重,刻不容缓。作为降碳减排的重要手段,钢铁生产碳排放数字化仿真目前主要集中于炼铁、炼钢等单一工序,存在着多工序碳排放模型缺乏、系统性流程改造方案欠缺等问题。为此,开展... 详细信息

对照我国提出的“双碳”战略,钢铁行业降碳减排时间紧,任务重,刻不容缓。作为降碳减排的重要手段,钢铁生产碳排放数字化仿真目前主要集中于炼铁、炼钢等单一工序,存在着多工序碳排放模型缺乏、系统性流程改造方案欠缺等问题。为此,开展了钢铁生产主工序流程碳排放数字化仿真及流程改造研究。首先,构建了“5+1”主工序流程碳排放数学模型,开发了华铸钢铁生产碳排放仿真软件,解决钢铁行业主工序流程模型缺乏问题;其次,基于宝钢和唐钢等企业数据进行验证,吻合度超96%,证实了模型与软件的准确性和可靠性,为流程改造奠定基础;最后,从技术革新、碳回收、碳中和和能源变更等四个方面,系统性提出了NTC(New Technology Combination)、燃烧后CO_(2.回收、燃烧前CO_(2.回收、氢冶金短流程生产等四种方案,技术实现从易到难,可依次服务于近期、中期、中后期和后期“双碳”计划,对钢铁行业降碳减排具有很好的参考作用。

关键词：碳排放数字化仿真流程改造钢铁生产降碳减排

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Structural parameterization and functional prediction of antigenic polypeptome sequences with biological activity through quantitative sequence-activity models (QSAM) by molecular electronegativity edge-distance vector (VMED)

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Science china(Life Sciences) 2007年第5期50卷 706-716页

作者： LI ZhiLiang1,2. WU ShiRong1,2. CHEN ZeCong1,2. YE Nancy1,2. YANG ShengXi1,2. LIAO ChunYang1,2. ZHANG MengJun1,2.3, YANG Li1,2. MEI Hu1,2.4, YANG Yan1,2. ZHAO Na1,2. zhou yuan1,2. zhou Ping1,2. XIONG Qing1,2. XU Hong1,2. LIU ShuShen1,2. LING ZiHua1,2. CHEN Gang1,2.4 & LI GenRong1,2.1 College of Chemistry and Chemical Engineering/Key Laboratory for Chemobiomedical Science and Engineering under Chongqing Municipality, College of Life Science and Biological Engineering/Key Laboratory for Biomechanics and Tissue Engineering under Ministry of Education, Chongqing university, Chongqing 400044, china 2.State Key Laboratory for Chemobiosensors and Chemobiometrics under MOST at Hunan university, Changsha 410012. china 3 Department of Medical Analysis/PLA center of Bioinformatics Immunology, Surgeon Third university, Chongqing 400031, china 4 Technology Centre for Life Sciences, Singapore Polytechnic, 500 Dover Road, Singapore 139651, Singapore 1. College of Chemistry and Chemical Engineering/Key Laboratory for Chemobiomedical Science and Engineering under Chongqing Municipality College of Life Science and Biological Engineering/Key Laboratory for Biomechanics and Tissue Engineering under Ministry of Education Chongqing University Chongqing 400044 China 2. State Key Laboratory for Chemobiosensors and Chemobiometrics under MOST at Hunan university Changsha 410012 China 3. Department of Medical Analysis/PLA center of Bioinformatics Immunology Surgeon Third University Chongqing 400031 China 4. Technology Centre for Life Sciences Singapore Polytechnic 500 Dover Road Singapore 139651 Singapore

Only from the primary structures of peptides, a new set of descriptors called the molecular electro-negativity edge-distance vector (VMED) was proposed and applied to describing and characterizing the molecular structures of oligopeptides and polypeptides, based on the electronegativity of each atom or electronic charge index (ECI) of atomic clusters and the bonding distance between atom-pairs. Here, the molecular structures of antigenic polypeptides were well expressed in order to propose the auto-mated technique for the computerized identification of helper T lymphocyte (Th) epitopes. Furthermore, a modified MED vector was proposed from the primary structures of polypeptides, based on the ECI and the relative bonding distance of the fundamental skeleton groups. The side-chains of each amino acid were here treated as a pseudo-atom. The developed VMED was easy to calculate and able to work. Some quantitative model was established for 2. immunogenic or antigenic polypeptides (AGPP) with 14 (1― 14) Ad and 14 other restricted activities assigned as "1"(+) and "0"(-), respectively. The latter comprised 6 Ab(15-2.), 3 Ak(2.-2.), 2.Ek(2.-2.), 2.H-2.(2. and 2.) restricted sequences. Good results were obtained with 90% correct classification (only 2.wrong ones for 2. training samples) and 100% correct prediction(none wrong for 8 testing samples); while con-trastively 100% correct classification (none wrong for 2. training samples) and 88% correct classification (1 wrong for 8 testing samples). Both stochastic samplings and cross valida-tions were performed to demonstrate good performance. The described method may also be suitable for estimation and prediction of classes I and II for major histocompatibility an-tigen (MHC) epitope of human. It will be useful in immune identification and recognition of pro-teins and genes and in the design and devel-opment of subunit vaccines. Several quantitative structure activity relationship (QSAR) models were developed for various oligop

关键词： molecular electronegativity distance-edge vector (VMED), antigenic polypeptide (AGPP) sequences, bioactive oligopeptide (BAOP) chains, quantitative sequence-activity models (QSAM), theoretically computational descriptors (TCD)

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Nano-micrometer surface roughness gradients reveal topographical influences on differentiating responses of vascular cells on biodegradable magnesium

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Bioactive Materials 2021年第1期6卷 262-272页

作者： Ke zhou Yutong Li Lei Zhang Liang Jin Feng yuan Jinyun Tan Guangyin yuan Jia pei National Engineering Research center of Light Alloy Net Forming&State Key Laboratory of Metal Matrix Composite School of Materials Science&EngineeringShanghai Jiao Tong University800 Dongchuan RoadShanghai200240China Department of Biomedical Engineering University of Texas at AustinTX78712USA Department of Vascular Surgery Huashan HospitalFudan UniversityShanghai200040China

Distinctively directing endothelial cells(ECs)and smooth muscle cells(SMCs),potentially by surface topography cue,is of central importance for enhancing bioefficacy of vascular *** the first time,surface gradients with a broad range of nano-micrometer roughness are developed on Mg,a promising next-generation biodegradable metal,to carry out a systematic study on the response of ECs and *** adhesion,spreading,and proliferation are quantified along gradients by high-throughput imaging,illustrating drastic divergence between ECs and SMCs,especially in highly rough *** profound role of surface topography overcoming the biochemical cue of released Mg2.is unraveled at different roughness ranges for ECs and *** insights into the underlying regulatory mechanism are gained at subcellular and gene *** work enables highefficient exploration of optimized surface morphology for modulating favored cell selectivity of promoting ECs and suppressing SMCs,providing a potential strategy to achieve rapid endothelialization for Mg.

关键词： Surface roughness gradients Topography cue Vascular cells Cell adhesion and proliferation Cell selectivity

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轨迹可控的微型水母机器人研制

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上海交通大学学报 2024年第8期58卷 1240-1248页

作者：王琦李绿洲董旭袁宁一丁建宁常州大学机械与轨道交通学院江苏常州213164 扬州大学机械工程学院扬州大学扬州碳中和科技创新中心江苏扬州225127 常州大学江苏省光伏科学与工程协同创新中心江苏常州213164

为解决微型机器人在较小机身尺寸下实现高效轨迹控制的难题,设计并研制了微型仿水母机器人JRT,其由气室、重心调整机构和仿水母推进器三部分组成,尺寸仅为6.8 cm×5.3 cm,可通过重心调整机构和仿水母推进器协同控制有效实现水下轨迹可控... 详细信息

为解决微型机器人在较小机身尺寸下实现高效轨迹控制的难题,设计并研制了微型仿水母机器人JRT,其由气室、重心调整机构和仿水母推进器三部分组成,尺寸仅为6.8 cm×5.3 cm,可通过重心调整机构和仿水母推进器协同控制有效实现水下轨迹可控.水下实验验证了机器人JRT沿竖直方向游泳和悬停、水平定向游泳以及水平弹射功能,并通过对机器人JRT沿竖直方向和水平方向游泳过程中的力平衡关系和运动状态数据分析提出了轨迹控制方案,测得水平定向游泳的速度可达到1.7 cm/s,轨迹偏差为0~3.4 cm,展现了微型仿水母机器人JRT较好的轨迹控制能力,可见其在水下资源勘查、环境监测等领域的应用潜力.

关键词：仿生机器人微型水母机器人电磁驱动轨迹控制运动分析重心调控

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Optimization on the Impeller of a Low-specific-speed Centrifugal Pump for Hydraulic Performance Improvement

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Chinese Journal of Mechanical Engineering 2016年第5期29卷 992-1002页

作者： pei Ji WANG Wenjie yuan Shouqi ZHANG Jinfeng National Research center of Pumps and Pumping System Engineering and TechnologyJiangsu University

In order to widen the high-efficiency operating range of a low-specific-speed centrifugal pump, an optimization process for considering efficiencies under 1.0Qd and 1.4Qd is proposed. Three parameters, namely, the blade outlet width b2. blade outlet angle β2. and blade wrap angle φ, are selected as design variables. Impellers are generated using the optimal Latin hypercube sampling method. The pump efficiencies are calculated using the software CFX 14.5 at two operating points selected as objectives. Surrogate models are also constructed to analyze the relationship between the objectives and the design variables. Finally, the particle swarm optimization algorithm is applied to calculate the surrogate model to determine the best combination of the impeller parameters. The results show that the performance curve predicted by numerical simulation has a good agreement with the experimental results. Compared with the efficiencies of the original impeller, the hydraulic efficiencies of the optimized impeller are increased by 4.18% and 0.62. under 1.0Qd and 1.4Qd, respectively. The comparison of inner flow between the original pump and optimized one illustrates the improvement of performance. The optimization process can provide a useful reference on performance improvement of other pumps, even on reduction of pressure fluctuations.

关键词： low-specific-speed centrifugal pump optimization optimal Latin hypercube sampling surrogate model particle swarm optimization algorithm numerical simulation

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