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Quantitative analysis of the ribbon synapse number of cochlear inner hair cells in C57BL/6J mice using the three-dimensional modeling method

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Science china(Life Sciences) 2009年第9期52卷 807-812页

作者： LIU Ke,LI ShuNa & JIANG XueJun Department of Otolaryngology,the First affiliated hospital,china medical University,Shenyang 110001,china Department of Otolaryngology the First Affiliated Hospital China Medical University Shenyang 110001 China

In mammals,the ribbon synapses of cochlear inner hair cells are a synaptic structure of the first sensory nerve in the pathway of acoustical signal transmission to the acoustic center,and it is directly involved in voice coding and neurotransmitter release. It is difficult to quantitatively analyze the ribbon synaptic number only using an electron microscope,because the ribbon synaptic number is relatively limited and their location is deep. In this study,the specific presynaptic structure-RIBEYE,and non-specific postsynaptic structure-GluR 2 & 3 in C57BL/6J mouse basilar membrane samples were treated by immunofluorescent labeling. Serial section was performed on the samples using a laser scanning confocal microscope,and then the serial sections were used to build three-dimensional models using 3DS MAX software. Each fluorescein color pair indicates one synapse,so the number of ribbon synapses of inner hair cells is obtained. The spatial distribution and the number of ribbon synapses of cochlear inner hair cells were clearly shown in this experiment,and the mean number of ribbon synapses per inner hair cell was 16.10±1.03. Our results have demonstrated the number of ribbon synapses is accurately calculated by double immunofluorescent labeling to presynaptic and postsynaptic structures,serial sections obtained using a laser scanning confocal microscope,and three-dimensional modeling obtained using 3DS MAX software. The method above is feasible and has important significance for further exploring the mechanism of sensorineural deafness.

关键词： inner hair cells Ribbon synapses laser scanning confocal microscope three-dimensional modeling

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Influence of rotating magnetic field on cerebral infarction volume, cerebral edema and free radicals metabolism after cerebral ischemia/reperfusion injury in rats

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Neural Regeneration Research 2006年第9期1卷 777-780页

作者： Xiaohong Liu1, Zhiqiang Zhang2, Lixin Zhang3 1Liaoning Disabled Children’s Rehabilitation Center, Shenyang 110015, Liaoning Province, china 2Department of Rehabilitation, Second hospital affiliated to china medical University, Shenyang 110003, Liaoning Province, china 3Department of Rehabilitation and Physiotherapy, First hospital affiliated to china medical University, Shenyang 110001, Liaoning Province, china Liaoning Disabled Children's Rehabilitation Center Shenyang 110015 Liaoning Province China Department of Rehabilitation China Medical University Shenyang 110003 Liaoning Province China Department of Rehabilitation and Physiotherapy China Medical University Shenyang 110001 Liaoning Province China

BACKGROUND: It has shown that magnetic field can improve blood circulation, decrease blood viscosity, inhibit free radicals, affect Ca2+ flow in nerve cells, control inflammatory and immunological reaction, and accelerate nerve cell regeneration. In addition, protective effect of magnetic field, which acts as an iatrophysics, on ischemic brain tissues has been understood gradually. OBJECTIVE: To investigate the effects of rotating magnetic field (RMF) on volume of cerebral infarction, cerebral edema and metabolism of free radicals in rats after cerebral ischemia/reperfusion injury. DESIGN: Randomized controlled animal study. SETTING: Rehabilitation Center of disabled children, Liaoniang; Department of Rehabilitation, the Second affiliated hospital, china medical University; Department of Rehabilitation Physiotherapy, the First affiliated hospital, china medical University. MATERIALS: A total of 70 healthy Wistar rats aged 18-20 weeks of both genders were selected and randomly divided into 3 groups: sham operation group with 12 rats, control group with 20 rats and treatment group with 38 rats. The treatment group included 4 time points: immediate reperfusion with 6 ones, 6-hour reperfusion with 20 ones, 12-hour reperfusion with 6 ones and 18-hour reperfusion with 6 rats. Main instruments were detailed as follows: magnetic head of rotating magnetic device was 6 cm in diameter; magnetic induction intensity at the surface of magnetic head was 0.25 T in silence; the maximal magnetic induction intensity was 0.09 T at the phase of rotation; the average rotating speed was 2500 r per minute. METHODS: The experiment was carried out in the china medical University in March 2003. Focal cerebral ischemic animal models were established with modified Longa’s method. Operation was the same in the sham operation, but the thread was inserted as 10 mm. Neurologic impairment was assessed with 5-rating method to screen out cases. Those survivals with grade 1 and grade 2 after ischemia f

关键词： cerebral edema and free radicals metabolism after cerebral ischemia/reperfusion injury in rats free

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不同剂量曲马朵超前镇痛用于七氟醚小儿麻醉的临床研究

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天津医药 2010年第4期38卷 279-281页

作者：宋丽施森魏继承欧册华泸州医学院附属医院麻醉科 646000 泸州医学院附属医院血管外科 646000

目的:探讨不同剂量曲马朵超前镇痛对学龄儿童七氟醚吸入麻醉的术后苏醒时间、拔管时间、循环、苏醒期躁动及镇静程度的影响。方法:选择行阑尾切除术的患儿90例,年龄6～10岁,ASAⅠ级,随机分为小剂量曲马朵组(T1)、大剂量曲马朵组(T2)及... 详细信息

目的:探讨不同剂量曲马朵超前镇痛对学龄儿童七氟醚吸入麻醉的术后苏醒时间、拔管时间、循环、苏醒期躁动及镇静程度的影响。方法:选择行阑尾切除术的患儿90例,年龄6～10岁,ASAⅠ级,随机分为小剂量曲马朵组(T1)、大剂量曲马朵组(T2)及对照组(C),每组30例。各组患儿以七氟醚、芬太尼和阿曲库铵诱导插管,T1、T2组分别于气管插管后静脉注射曲马朵1mg/kg、2mg/kg,C组静脉注射生理盐水,术中吸入七氟醚维持麻醉,术毕待患儿清醒拔管。结果:各组患儿呼唤睁眼时间、拔管时间和脉搏氧饱和度(SpO2)比较差异无统计学意义。与C组相比,T2组患儿拔管时及拔管后10min的心率(HR)、平均动脉压(MAP)明显降低,术后躁动发生率明显下降,术后镇静满意率明显升高(P0.05)。结论:2mg/kg的曲马朵超前镇痛可以有效预防学龄儿童七氟醚吸入麻醉术后躁动的发生,获得较为满意的镇静效果,拔管时及拔管后循环更平稳,且不影响术后苏醒时间和拔管时间。

关键词：曲马朵醚类麻醉,吸入镇痛麻醉恢复期儿童(6-12)

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灰毡毛忍冬的生药学研究

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时珍国医国药 2005年第11期16卷 1076-1077页

作者：税丕先刘家奇孙琴庄元春泸州医学院药学院四川泸州646000 四川省泸州市中医医院 646000

目的:为灰毡毛忍冬的鉴别,开发利用提供依据。方法:采用来源、性状、显微、理化对其鉴别。结果:首次系统阐明了灰毡毛忍冬的生药学理论。结论:为制订灰毡毛忍冬质量标准提供依据。

关键词：灰毡毛忍冬来源性状显微理化鉴别

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儿童变应性鼻炎严重程度与外周血嗜酸细胞阳离子蛋白、内皮素和肿瘤坏死因子-α的水平变化的关系

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中国妇幼保健 2013年第1期28卷 147-149页

作者：姜梁胡晓艳泸州医学院附属医院耳鼻咽喉头颈外科四川泸州646000 泸州医学院病原生物学教研室

目的:研究儿童变应性鼻炎的严重程度与患者外周血嗜酸细胞阳离子蛋白(ECP)、内皮素(ET)和肿瘤坏死因子-α(TNF-α)水平的关系,探讨ECP、ET与TNF-α在儿童变应性鼻炎进展中的作用。方法:选择2010年3月～2011年3月急性发作期的变应性鼻炎... 详细信息

目的:研究儿童变应性鼻炎的严重程度与患者外周血嗜酸细胞阳离子蛋白(ECP)、内皮素(ET)和肿瘤坏死因子-α(TNF-α)水平的关系,探讨ECP、ET与TNF-α在儿童变应性鼻炎进展中的作用。方法:选择2010年3月～2011年3月急性发作期的变应性鼻炎患儿136例作为研究对象,根据症状与体征情况分为轻度组93例与重度组43例,另外选取40例健康儿童作为对照组。使用ELISA双抗体夹心法检测对照组、轻度组、重度组中ECP、ET与TNF-α的水平。结果:对照组、轻度组与重度组中的ECP水平分别为(12.85±1.94)pg/ml、(25.86±3.07)pg/ml、(30.82±3.25)pg/ml;ET水平分别为(53.28±10.12)pg/ml、(82.44±22.43)pg/ml、(99.28±30.16)pg/ml;TNF-α的水平分别为(18.79±4.96)pg/ml、(31.87±9.86)pg/ml、(51.26±11.14)pg/ml。与对照组相比,轻度组与重度组的ECP、ET与TNF-α水平均明显偏高(P0.05)。结论:外周血嗜酸细胞阳离子蛋白、内皮素和肿瘤坏死因子-α水平在儿童变应性鼻炎的进展中具有重要作用。

关键词：变应性鼻炎嗜酸细胞阳离子蛋白内皮素肿瘤坏死因子-α

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Effect of cerebral lymphatic block on cerebral morphology and cortical evoked potential in rats

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Neural Regeneration Research 2006年第7期1卷 657-660页

作者： Zuoli Xia1, Baoliang Sun2, Mingfeng Yang1, Dongmei Hu2, Tong Zhao2, Jingzhong Niu2 1Institute of Brain Microcirculation, Taishan medical college, Taian 271000, Shandong Province, china 2Department of Neurology, affiliated hospital of Taishan medical college, Taian 271000, Shandong Province, china Institute of Brain Microcirculation Taishan Medical College Taian 271000 Shandong Province China Department of Neurology Hospital of Taishan Medical College Taian 271000 Shandong Province China

BACKGROUND: It has been shown that although brain does not contain lining endothelial lymphatic vessel, it has lymphatic drain. Anterior lymphatic system of lymphatic vessel in brain tissue plays a key role in introducing brain interstitial fluid to lymphatic system; however, the significance of lymphatic drain and the effect on cerebral edema remains unclear. OBJECTIVE: To investigate the effect of cerebral lymphatic block on cerebral morphology and cortical evoked potential in rats. DESIGN: Randomized controlled animal study. SETTING: Institute of Cerebral Microcirculation of Taishan medical college and Department of Neurology of affiliated hospital. MATERIALS: A total of 63 healthy adult male Wistar rats weighing 300-350 g were selected in this study. Forty-seven rats were used for the morphological observation induced by lymphatic drain and randomly divided into three groups: general observation group (n =12), light microscopic observation group (n =21) and electronic microscopic observation group (n =14). The rats in each group were divided into cerebral lymphatic block subgroup and sham-operation control subgroup. Sixteen rats were used for observing the effect of cerebral lymphatic block on cortical evoked potential, in which the animals were randomly divided into sham-operation group (n =6) and cerebral lymphatic block group (n =10). METHODS: The experiment was carried out in the Institute of Cerebral Microcirculation of Taishan medical college from January to August 2003. Rats in cerebral lymphatic block group were anesthetized and separated bilateral superficial and deep cervical lymph nodes under sterile condition. Superior and inferior boarders of lymph nodes were ligated the inputting and outputting channels, respectively, and then lymph node was removed so as to establish cerebral lymphatic drain disorder models. Rats in sham-operation control group were not ligated the lymphatic vessel and removed lymph nodes, and other operations were as the same as

关键词： Effect of cerebral lymphatic block on cerebral morphology and cortical evoked potential in rats

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Optical coherence tomography assessed retinal nerve fiber layer thickness in patients with Alzheimer's disease:a meta-analysis

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International Journal of Ophthalmology(English edition) 2012年第3期5卷 401-405页

作者： Xue-Fei He 1,Yi-Ting Liu 2,Cheng Peng 1,Fan Zhang 1,Shi Zhuang 1,Jin-Song Zhang 1 1Key Laboratory of Lens Research Liaoning Province,Department of Ophthalmology,Eye hospital of china medical University,the Fourth affiliated hospital of china medical University Shenyang 110005,Liaoning Province,china 2 Department of Clinical Epidemiology,the First affiliated hospital,china medical University,Shenyang 110001,Liaoning Province,china Key Laboratory of Lens Research Liaoning Province Department of Ophthalmology Eye Hospital of China Medical University the Fourth Affiliated Hospital of China Medical University Shenyang 110005 Liaoning Province China Department of Clinical Epidemiology the First Affiliated Hospital China Medical University Shenyang 110001 Liaoning Province China

AIM:To investigate the difference of retinal nerve fiber layer(RNFL) thickness between Alzheimer's disease patients and normal people,so as to provide clue for the early diagnosis of Alzheimer's disease.· METHODS:The articles on the association of RNFL thickness and Alzheimer's disease were retrieved by searching international and national *** qualified articles were assessed by meta analysis with Stata11.0 *** results were pooled using weighted mean difference(WMD) with a corresponding 95% confidence interval(CI).· RESULTS:Totally 7 studies enrolled 324 eyes were included in the *** results of meta analysis showed that in AD patients,there was a significant average RNFL thickness reduction compared with the control group [WMD=-17.561,95%CI:(-23.971,-11.151)].There were significant differences in superior,inferior,nasal and temporal RNFL thickness between the two *** with a 95%CI were [-18.829,95%CI:(-25.915,-11.743);P25.775,95%CI:(-34.304,-17.247);P 29.141,-4.613);P28.002,-1.128);P<0.001] ***'s test and Egger's test did not show significant difference,funnel plot was basically symmetrical,indicating that there was no publication bias existed.· CONCLUSION:There are significant differences in the RNFL thickness in all quadrants between the two *** thickness is reduced in AD patients compared with the control group.·

关键词： retinal nerve fiber layer thickness optical coherence tomography Alzheimer's disease meta analysis

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Refractive Changes in Chicks with Form-deprivation Myopia

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Eye Science 1996年第3期16卷 138-139页

作者： Fubin Wang, Tao Chu, Wei ZhangDepartment of Ophthalmology, the First affiliated hospital of Qiqihar medical college Qiqihar 161041, china Department of Ophthalmology First Affiliated Hospital of Qiqihar Medical College China.

Purpose: To study the refractive changes of form deprivation of myopia. Methods: Haisaik chickens were used to establish the animal models of myopia. Monocular eyelids were sutured for form deprivation on the 5th day after chickens birth. The chickens were examined by optometer and the anteroposterior axis, transverse and vertical diameters were measured by verniermicrometer. Results: Form deprivation leads to myopic refractive abnormality (P2:138-139.

关键词： form deprivation myopia refraction

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Effect of calcitonin gene-related peptide and nerve growth factor on spatial learning and memory abilities of rats following focal cerebral ischemia/reperfusion

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Neural Regeneration Research 2006年第8期1卷 673-676页

作者： Guangshun Zheng1, Yongjie Yang2, Xiubin Fang3 1Department of Neurosurgery, Second hospital of Xiamen, Xiamen 361021, Fujian Province, china 2Department of Neurosurgery, Second hospital affiliated to china medical University, Shenyang 110004, Liaoning Province, china 3Department of Neurobiology, Basic medical college of china medical University, Shenyang 110001, Liaoning Province, china Department of Neurosurgery Second Hospital of Xiamen Xiamen 361021 Fujian Province China Department of Neurosurgery China Medical University Shenyang 110004 Liaoning Province China Department of Neurobiology Basic Medical College China Medical University Shenyang 110001 Liaoning Province China

BACKGROUND: Calcitonin gene-related peptide (CGRP) and nerve growth actor (NGF) cam improve spatial learning and memory abilities of rats with cerebral ischemia/reperfusion; however, the effect of combination of them on relieving learning and memory injury following cerebral ischemia/reperfusion should be further studied. OBJECTIVE: To study the effects of exogenous CGRP and NGF on learning and memory abilities of rats with focal cerebral ischemia/reperfusion. DESIGN: Randomized controlled animal study. SETTING: Department of Neurosurgery, the Second hospital of Xiamen; Department of Neurosurgery, the Second affiliated hospital of china medical University; Department of Neurobiology, Basic medical college of china medical University. MATERIALS: A total of 30 healthy male SD rats, aged 8 weeks, of clean grade, weighing 250-300 g, were provided by Experimental Animal Department of china medical University. All rats were randomly divided into sham-operation group, ischemia/reperfusion group and treatment group with 10 in each group. The main reagents were detailed as the follows: 100 g/L chloral hydrate, 0.5 mL CGRP (2 mg/L, Sigma Company, USA), and NGF (1× 106 U/L, 0.5 mL, Siweite Company, Dalian). METHODS: The experiment was carried out in the Department of Neurobiology, Basic medical college of china medical University from February to July 2005. Rat models of middle cerebral artery occlusion were established by method of occlusion, 2 hours after that rats were anesthetized and the thread was slightly drawn out for 10 mm under direct staring to perform reperfusion. Rats in the ischemia/reperfusion group received intraperitoneal injection of 1 mL saline via the abdomen at two hours later, while rats in the treatment group at 2 hours later received intraperitoneal injection of 2 mg/L CGRP (0.5 mL) and 1×106 U/L NGF (0.5 mL) once a day for 10 successive days. First administration was accomplished within 15 minutes after ischemia/reperfusion. Rats in the sham-operation

关键词： Effect of calcitonin gene-related peptide and nerve growth factor on spatial learning and memory abilities of rats following focal cerebral ischemia/reperfusion CGRP MCAO gene

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胱抑素与老年肌酐正常肾损害患者肾小球滤过率的相关性研究

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中国实用内科杂志 2005年第8期25卷 725-726页

作者：刘建杭永伦侯静李友芸泸州医学院附属医院肾病内科 6460002 泸州医学院附属医院检验科 6460002

目的探讨在血清肌酐正常的老年肾损害患者中,胱抑素(cystatin C,Cys-C)对肾小球滤过率(GFR)评价的意义.方法对2002-11～2003-12泸州医学院附属医院86例有肾损害但血清肌酐(Scr)正常的老年患者,应用乳胶颗粒增强比浊法测定血清中Cys-C浓... 详细信息

目的探讨在血清肌酐正常的老年肾损害患者中,胱抑素(cystatin C,Cys-C)对肾小球滤过率(GFR)评价的意义.方法对2002-11～2003-12泸州医学院附属医院86例有肾损害但血清肌酐(Scr)正常的老年患者,应用乳胶颗粒增强比浊法测定血清中Cys-C浓度,并采用CockCroft-Cault公式计算内生肌酐清除率(Ccr).分别比较Cys-C与Scr和Ccr的相关性.结果 56例Cys-C升高超过正常,占56.1%;比较Ccr≤59 mL/min和Ccr>59 mL/min两组间的Cys-C质量浓度发现,当Ccr≤50 mL/min时,97.1%的患者Cys-C超过正常,在Ccr>59 mL/min时,已有44.2%患者Cys-C超过正常.血清Cys-C与Scr存在高度的正直线相关(r=0.89,P<0.01);Cys-C与Ccr存在高度的负直线相关(r=-0.87,P<0.01).结论在老年患者中,Cys-C比Scr和Ccr更敏感地反映早期肾小球滤过率的下降.

关键词： Cystatin C 肌酐清除率肾小球滤过率

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