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机采血小板22℃保存理化功能改变的分析

四川医学 2009年第3期30卷 335-337页

作者：李小平李代渝赵华张洪为川北医学院附属医院输血科四川南充637000 泸州医学院附属医院输血科检验科四川泸州646000

目的观察分析机采血小板室温保存期间的代谢变化损伤,为临床血小板输注评价提供可参考指标。方法血小板室温22℃保存0、3、5、7d,分别检测血小板的代谢情况,形态改变,功能变化等一系列指标,对血小板的保存损伤进行多参数分析。结果血小... 详细信息

目的观察分析机采血小板室温保存期间的代谢变化损伤,为临床血小板输注评价提供可参考指标。方法血小板室温22℃保存0、3、5、7d,分别检测血小板的代谢情况,形态改变,功能变化等一系列指标,对血小板的保存损伤进行多参数分析。结果血小板保存从3d开始乳酸(LA)、乳酸脱氢酶(LDH)堆积增多,平均血小板体积(MPV)、血小板分布宽度(PDW)数值逐渐增大,形态改变明显,葡萄糖(Glu)、pH值则呈下降趋势,CD62P表达增强,血小板活化明显,聚集功能低下,保存到5、7d所观察的各项指标变化更加明显,聚集功能几乎下降为零。用0d的各数值同3、5、7d比较差异均具有统计学意义(P<0.01)。结论血小板保存期间形态功能改变明显,临床在做血小板输注效果评价时,应当考虑血小板本身的质量问题。特别是在保存3d后,血小板具有明显的损伤,聚集功能显著减弱,临床输注保存期的血小板最佳时间为3d。

关键词：机采血小板室温保存临床评价保存损伤

新生儿窒息后多器官功能障碍综合征的临床研究

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四川医学 2009年第4期30卷 453-454页

作者：张本金董文斌雷小平王胜会李清平翟雪松郭琳达州市中心医院儿科四川达州635000 泸州医学院附属医院新生儿科四川泸州646000

目的研究新生儿窒息后多器官功能障碍综合征(multipleorgandysfunctionsyndrome,MODS)的发生及其与病情预后的关系。方法窒息后24h之内入院的足月新生儿89例,进行经皮血氧饱和度和心电监测3d,同时作肝功、肾功、心肌酶、电解质、血常规... 详细信息

目的研究新生儿窒息后多器官功能障碍综合征(multipleorgandysfunctionsyndrome,MODS)的发生及其与病情预后的关系。方法窒息后24h之内入院的足月新生儿89例,进行经皮血氧饱和度和心电监测3d,同时作肝功、肾功、心肌酶、电解质、血常规、尿常规、血气分析及心电图和头颅CT等检查,结合临床表现统计窒息后全身炎症反应综合征(systemic inflammatory respons syndrome,SIRS)和MODS的发生情况及其疾病的转归情况。结果与轻度窒息组相比,重度窒息组危重病评分、SIRS和MODS发生率以及多器官损伤的程度明显增加,差异有统计学意义(P<0.05);与预后好组相比,预后差组SIRS和MODS发生率明显增高(P<0.05)。结论新生儿窒息可导致SIRS和MODS的发生,与窒息程度和疾病的预后密切相关。

关键词：新生儿窒息全身炎症反应综合征多器官功能障碍综合征

STAT1 Antisense Oligonucleotides Attenuate the Proinflammatory Cytokine Release of Alveolar Macrophages in Bleomycin-Induced Fibrosis

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Cellular & Molecular Immunology 2005年第3期2卷 211-217页

作者： Xianming Fan~(1,3) Zengli Wang~2 ~1Department of Respiratory Medicine,the affiliated hospital of luzhou medical college,luzhou 646000,china ~2Department of Respiratory Medicine,West china hospital,Sichuan University,Chengdu 610041,china ~3Corresponding to:Dr.Xianming Fan,Department of Respiratory Medicine,the affiliated hospital of luzhou medical college,luzhou 646000,china. Department of Respiratory Medicine the Affiliated Hospital of Luzhou Medical College Luzhou 646000 China. fxm129@***

To investigate the effect of signal transducers and activators of transcription 1 （STAT1） antisense oligonucleotides （ASON） on concentrations of TNF-α IL-8, NO secreted by alveolar macrophages （AMs） in bleomycin-induced rat pulmonary fibrosis, five adult female Wistar rats were intratracheally instilled with bleomycin. After 7 days, the rats were killed by right ventricle of heart exsanguinations under ketamine anaesthesia and bronchoalveolar lavage （BAL） was performed to obtain AMs. AMs were divided into four groups, treated with STAT1 ASON, STAT1 sense oligonucleotides （SON）, dexamethasone （DEX） and medium alone （control）, respectively. AMs and media were collected after culture for 36 h. The mRNA and protein expressions of STAT1 and ICAM-1 in AMs were detected by RT-PCR and ELISA, respectively. The concentrations of TNF-α IL-8, NO in cultured medium were detected. The STAT1 mRNA expression by AMs in the STAT1 ASON group was lower than those of AMs in the STAT1 SON group, the DEX group and the control group （p 〈 0.05）. Moreover, the STAT1 mRNA expression by AMs in the DEX group was also lower than those of AMs in the STAT1 SON group and the control group （p 〈 0.05）, but the STAT1 mRNA expression by AMs in the STAT1 SON group was not different from that of the control group （p 〉 0.05）. The protein expressions of STAT1 and ICAM-1 and the mRNA expression of ICAM-1 showed similar changes to the STAT1 mRNA expression by AMs. The concentrations of TNF-α IL-8, NO in cultured medium from STAT1 ASON group were lower than those from STAT1 SON, DEX and the control groups （p 〈 0.05）. Moreover, the concentrations of TNF-α IL-8, NO in cultured medium from DEX group were also lower than those from the control and STAT1 SON group （p 〈 0.05）, but no difference between STAT1 SON group and the control （p 〉 0.05）. The results suggest that STAT1 ASON could inhibit the secretion of TNF-α IL-8, NO in AMs, and STAT1 could become a target of treating pulmonary fibrosis. Cellular ＆ Molecul

关键词： STAT1 pulmonary fibrosis alveolar macrophage ASON

Diagnosis and treatment of hepatic cholangiocarcinoma:report of 52 cases

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Hepatobiliary & Pancreatic Diseases International 2003年第1期2卷 62-65页

作者： Zi-Jian Guo Qiu Li Kai He the Department of Hepatobiliary Surgery, affiliated hospital, luzhou medical Gollege, luzhou 646000, china Department of Hepatobiliary Surgery Affiliated Hospital Luzhou Medical College Luzhou 646000 China. lilihua 166@***

OBJECTIVE: To summarize the experience in diagnosis and surgical treatment of hepatic cholangiocarcinoma. METHODS: Clinical features, diagnosis, surgical treatment and prognosis of 52 patients with hepatic cholangiocarcinoma treated at our hospital from 1993 to 2001 were retrospectively reviewed. RESULTS: The patients with hepatic cholangiocarcinoma accounted for 4.1% (52/1261) of the total patients with primary, liver carcinoma encountered at this hospital. The ratio of male to female was 1.36:1 in this group. Some patients were complicated by hepatitis B (32.7%), hepatolith (34.6%), liver abscess (13.5%), cirrhosis (53.8%), and serum positivity for alpha-fetaprotein (21.2%). No typical clinical manifestations were present in all patients. The diagnostic rates of ultrasound examination and CT were 80.8% and 94.2%, respectively. The resection rate of hepatic cholangiocarcinoma was 48.1%; and the 1-, 3-, 5-year survival rates were 48.1% (25/52), 30.8% (16/52), and 19.2% (10/52) respectively in this group. In resectable cases, the 1-, 3-, 5-year survival rates were 80.0% (20/25), 48.0% (12/25), 28.0% (7/25) respectively. Seven patients without cirrhosis who had received radical resection and intra-arterial chemotherapy survived for more than 5 years. In unresectable 27 cases, only 5 survived over one year even if selective hepatic arterial embolism and perfusion chemotherapy were employed. CONCLUSIONS: No specific, serum marker is found for hepatic cholangiocarcinoma. CT diagnosis is superior to ultrasonography. When a patient has already diagnosed as having hepatolith or liver abscess before operation and occupying lesion is seen intraoperatively, rapid pathological examination should be done for a fair judgement of the nature of the mass and a decision-making of operative protocol. The patient can survive well after curative resection. No matter whether hepatic cholangiocarcinoma is resectable or not, both selective hepatic arterial embolism and perfusion chemothera

关键词： liver neoplasm hepatic resection

Effects of human umbilical cord serum on proliferation and insulin content of human fetal islet-like cell clusters

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Hepatobiliary & Pancreatic Diseases International 2004年第1期3卷 144-148页

作者： Dong Xia, Hong-Yuan He, Zheng-Ming Lei, Pei-Ming Zhang and Yong Guo luzhou, china Department of General Surgery, West china hospital , and Department of Surgery, Fifth hospital of Sichuan Province , Chengdu 610041, china Department of Histology and Em- bryology , and Hepatobiliary and Pancreatic Laboratory , luzhou medical college, luzhou 646000, china Department of General Surgery West China Hospital Chengdu 610041 China. juliahhy@***

BACKGROUND: Type 1 diabets is an autoimmune disease caused by the destruction of pancreatic β-cell with an in- creased incidence worldwide in the closing decades of the 20th century. This study was to investigate the effects of human umbilical cord serum (UCS) on the proliferation and function of human fetal islet-like cell clusters (ICCs) in vitro. METHODS: Eight fresh pancreatic glands obtained after in- duction of labor with water bag were mildly exposed to col- lagenase V, and the digested cells were cultured in a RPMI- 1640 medium plus 10% pooled UCS or fetal calf serum (FCS) to permit cells attachment and outgrowth of ICCs. RESULTS: In 8 consecutively explanted glands, develop- ment and proliferation of ICCs were observed. In the pre- sence of FCS, the outgrowth of ICC took place on the top of a flbroblast monocellular layer. UCS affected less growth of fibroblasts and increased the formation of ICCs about four-fold compared with explants from the same glands maintained in FCS. In both UCS and FCS, the insulin con- tent of the medium was variable to a certain extent and progressively declined from day 2 to day 6. Dithizone- stained ICCs in UCS suggested that most cell clusters were islet cells ( β-cells), and the purity of islets was estimated 80%-90%. The ultrastructure of the cultured cells showed a large number of granule-containing cells, most of which were identified as β-cells. CONCLUSION: We conclude that in comparison with ex- plants with FCS, the yield of ICCs and purification of islet cells are markedly increased by UCS and may facilitate the proliferation of pancreatic β-cells intended for islet trans- plantation.

关键词： islet cell culture islet-like cells clusters umbilical cord serum fetal calf serum

Effects of multidrug resistance, antisense RNA on the chemosensitivity of hepatocellular carcinoma cells

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Hepatobiliary & Pancreatic Diseases International 2006年第4期5卷 552-559页

作者： Bo Li, Tian Ye, Lei Zhao, De-Hua Li, Xing-Hua Gou, Lan-Ying Zhao, Lei Han, Lin Chen, Lu-Nan Yan and Jian-Ping Gong Department of Hepatobiliary Surgery, Second affiliated hospital, Chongqing University of medical Sciences, Chongqing 400010, china Department of Traditional Chinese Surgery, Second affiliated hospital, luzhou medical college, luzhou 646000, china Genetic Engineering Laboratory, Chengdu Di’ao Group Co., Ltd., Chengdu 610041, china Department of General Surgery, West china hospital, Sichuan University, Chengdu 610041, china Department of Hepatobiliary Surgery Second Affiliated Hospital Chongqing University of Medical Sciences Chongqing 400010 China.

BACKGROUND: Multidrug resistance is a major obstacle in cancer chemotherapy. We examined whether the antisense RNA of multidrug resistance gene 1 (mdr1) could reverse multidrug resistance in the human hepatocellular carcinoma (HCC) cell line SMMC7721/ADM. METHODS: The recombinant adenoviruses pAdEasy- GFP-ASmdr1 product was produced by the adenoviral vector AdEasy system, which can express antisense RNA against the mdr1 gene. Following that, the recombinant adenovirus was transfected into the P-glycoprotein- producing multidrug resistance cell line, SMMC7721/ADM human HCC cells resistant to adriamycin (ADM) and daunorubicin (DNR). In order to investigate the reversal of multidrug resistance phenotype, we measured the expression of mdr1 mRNA by RT-PCR and the production of P-glycoprotein by flow cytometry. The sensitivities for ADM and DNR SMMC7721/ADM cells were examined by [3-(4, 5-dimethylthi-azol-2-yl)-2,5 diphenyl-terazolium bromide] (MTT) analysis. RESULTS: The low-level expression of mdr1 mRNA and P-glycoprotein production were observed in parental sensitive cells SMMC/7721 in addition to the overexpressionof mdr1 mRNA and P-glycoprotein in SMMC7721/ADM cells. The transfection of antisense-RNA into SMMC7721/ ADM cells resulted in decreases of mdr1 mRNA and P-glycoprotein, but increase of drug sensitivities. The sensitivities of transfected SMMC7721/ADM cells to ADM and DNR in IC50 reduced by 31.25% and 62.96% respectively. CONCLUSIONS: Mdr1 antisense RNA can increase the sensitivities of SMMC7721/ADM cells to anticancer drug by decreasing the expression of the mdr1 gene and inhibiting P-glycoprotein expression. This strategy may be applicable to cancer patients with P-glycoportein mediated multidrug resistance.

关键词： antisense nucleic acid multiple drug resistance P-glycoprotein

Phytoestrogen genistein decreases contractile response of aortic artery in vitro and arterial blood pressure in vivo

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Acta Pharmacologica Sinica 2004年第3期25卷 59-64页

作者： Hong-fang LI1,Long-de WANG2,Song-yi QU Department of Physiology,Lanzhou medical college,Lanzhou 730000 2affiliated hospital,Gansu Chinese medical college,Lanzhou 730020,china Department of Physiology Lanzhou Medical College affiliated hospital Gansu Chinese Medical College

AIM: To determine the mechanisms of effects of phytoestrogen genistein on the contracted rabbit aortic arteries in vitro, and observe the effect of genistein and 17-β estradiol on mean arterial pressure (MAP) in ovariectomized (OVX) rats. METHODS: (1) Strips of rabbit aortic smooth muscle were suspended in organ baths containing Kreb’s solution, and then isometric tension was measured. (2) Female mature Wistar rats underwent a bilateral ovariectomy (OVX). Sham-operated rats (SHAM) were used as controls. After administration of genistein (0.4 mg·kg-1·d-1, sc), 17-β estradiol (1 mg·kg-1·d-1, sc) or theirvehicle sesame oil for 21 d,MAP was measured. RESULTS: (1) Similar to 17-β estradiol, genistein could dose-dependently relax 40 mmol/L KCl-precontracted arterial strips. Incubation with Nω-L-nitro-arginine (L-NNA), methylene blue (MB), indomethacin, propranolol or endothelium removal did not affect relaxation induced by genistein. In calcium-free solution containing 0.01mmol/L egtazic acid (EGTA), genistein inhibited not only the first phase contraction induced by noradrenaline (NA), but also the second contraction induced by CaCl2. In addition, genistein could reduce the contractile responses of NA, KCl and CaCl2, and shift their cumulative concentration-response curves rightward. (2) MAP in OVX rats was significantlyhigher compared with that of SHAM rats. However,after chronically treatment with genistein or 17-β estradiol for 21 d the baseline MAP in OVX rats was reduced significantly. CONCLUSIONS: (1) The vasodilator effect of genistein in vitro is endothelium independent and not related to the nitric oxide, its mechanisms being probably due to inhibition of Ca2+ influx through calcium channels in a noncompetitive manner and Ca2+ release from intracellular store induced by NA. (2) Administration of genistein or 17-β estradiol can chronically decrease MAP in OVX rats.

关键词： genistein aorta smooth muscle muscle relaxation calciumchannel vascular endothelium blood pressure

Sodium dimercaptopropane sulfonate as antidote against non-metallic pesticides

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Acta Pharmacologica Sinica 2004年第4期25卷 136-146页

作者： Zhi-kang CHEN, Zhong-qiu LU1,2 Department of Pharmacology, School of Pharmacy, Wenzhou medical college, Wenzhou 325035 2Emergency Department, TheFirst affiliated hospital of Wenzhou medical college, Wenzhou 325000, china Department of Pharmacology School of Pharmacy Wenzhou Medical College Emergency Department The First Affiliated Hospital of Wenzhou Medical College China

With the advent of World War II, dimercaptol was first developed in Englandas an effective antidote against arsenical agents. In 1950''s, scientists from the Soviet Uniondeveloped a water-soluble compound, sodium dimercaptopropane sulfonate (Na-DMPS) named as Unithiol(or Unitiol), which was able to chelate heavy metals and metalloids. Unithiol was collected in thePharmacopeia of the Soviet Union. After a lot of efforts of scientists from several countries, thisdrug has been widely used in the treatment of the poisoning of heavy metals and metalloids, as awell-known specific antidote. Considering the importance of sulfhydryl groups in human body, wefirst initiated the possibility of sulfhydryl compounds as antidotes against some non-metaltoxicants. In 1970''s, they screened many sulfhydryl compounds as antidotes against acute poisoningof nereistoxin insecticides (NTXI), and found that dithiol compounds were much better than monothiolcompounds in the treatment for NTXI poisoning. Na-DMPS and sodium dimercapto-succinate (Na-DMS)were among the best antidotes (Fig 1). The accumulation of experimental studies and clinical trialshas confirmed that they were specific effective antidotes against NTXI. In 1990''s, Na-DMPS was firstapplied to treat the acute poisoning of chlordimeform (CDM) an organoni-trogen insecticide, andbactericide 402, an organosulfur pesticide, and tetramine, a rodenticide. Through thoroughpharmacological and toxicological studies of these non-metallic pesicides, the application ofNa-DMPS as an antidote has been widened, and the development of antidotes against pesticides hasbeen prospected. The application of Na-DMPS as an antidote against non-metallic pesticides is asuccessful example, which shows important theoretical basis and practical value.

关键词： sodium dimercaptopropane sulfonate antidotes pesticides nereistoxin insecticides chlordimeform bactericide 402 tetramine poisoning

THERAPEUTIC EFFECTS OF HEAD ACUPUNCTURE ON STROKE

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World Journal of Acupuncture-Moxibustion 1994年第2期4卷 3-6页

作者： Zhou Jianwei, Zhang FanTCM hospital of luzhou medical college, Sichuan 646000, china

According to the "International Standard of Chinese Head Acupoints" 207 patientssuffering from stroke were treated by needling along the anterior and posterior obligue lines of vertex-temporal, and the line 1 and line 2 lateral to vertex. The markedly effective rate accounted for73. 43% of the total, and the overall effective rate represented 89. 86%. After the treatment somesymptoms as hemiplegia, lingual dysfunction, facial and tongue paralysis were obviously improved (P< 0. 01 ). The therapeutic effect of head acupuncture was obviously better than that of Westernmedicine(P<0.01). Comparison of therapeutic effects made among needling along different lines andbetween those applied on the intact and affected sides of the body showed that they were all effectivein improving the symptoms, but there was no significant difference(P<0. 05)

关键词： Stroke Cerebrovascular disorders Head acupuncture