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生物物理学报 2009年第S1期25卷 217-218页

作者： Shusen Liu state key laboratory of biomembrane and membrane Biotechnology, Instutute of zoology, chinese academy of sciences, beijing, 100101, china

Although some fusogenic proteins have been established to tether two opposite outer or inner mitochondrial membranes into contact, a prerequisite for whole mitochondrial fusion events,

关键词： Mitochondrial membrane fusion Protein-tethering Lipid-bilayer-mixing and fusion Interfacial △pH of PMF.Inter-play between membrane fusion and ATP synthesis.

Dynamics of morphological changes for mitochondrial fission and fusion

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Science china(Physics,Mechanics & Astronomy) 2010年第4期53卷 680-689页

作者： WANG ShiQi 1,2,4 , FU ChangLiang 1,2 , ZHANG Yan 1,2 , CHEN Quan 3 & LONG Mian 1,2 1 key laboratory of Microgravity (National Microgravity laboratory), beijing 100190, china 2 Center for Biomechanics and Bioengineering, institute of Mechanics, chinese academy of sciences, beijing 100190, china 3 state key laboratory of biomembrane and membrane Biotechnology, institute of zoology, chinese academy of sciences, beijing 100101, china 4 institute of Vertebrate Paleontology and Paleoanthropology, chinese academy of sciences, beijing 100044, china key laboratory of Microgravity (National Microgravity laboratory) Beijing China Center for Biomechanics and Bioengineering Institute of Mechanics Chinese Academy of Sciences Beijing China institute of Vertebrate Paleontology and Paleoanthropology Chinese Academy of Sciences Beijing China state key laboratory of biomembrane and membrane Biotechnology Institute of Zoology Chinese Academy of Sciences Beijing China

Mitochondria experience continuous fusion and fission in a living cell, but their dynamics remains poorly quantified. Here a theoretical model was developed, upon a simplified population balance equation (PBE), to predict the morphological changes induced by mitochondrial fission and fusion. Assuming that both fission and fusion events are statistically independent, the survival probability of mitochondria staying in the fission or fusion state was formulated as an exponentially-decayed function with time, which depended on the time-dependent distribution of the mitochondrial volume and the fission and fusion rates. Parametric analysis was done for two typical volume distributions. One was Gamma distribution and the other was Gaussian distribution, derived from the measurements of volume distribution for individual mitochondria in a living cell and purified mitochondria in vitro. The predictions indicated that the survival probability strongly depended on morphological changes of individual mitochondria and was inversely correlated to the fission and fusion rates. This work provided a new insight into quantifying the mitochondrial dynamics via monitoring the evolution of the mitochondrial volume.

关键词： mitochondria morphology fission fusion dynamics modeling

Molecular basis of cryptorchidism-induced infertility

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Science china(Life sciences) 2010年第11期53卷 1274-1283页

作者： LIU YiXun & LI XiXia state key laboratory of Reproductive Biology, institute of zoology, chinese academy of sciences, beijing 100101, china state key laboratory of Reproductive Biology Institute of Zoology Chinese Academy of Sciences Beijing China

Artificial cryptorchidism or local testicular heat treatment can induce reversible oligospermia or azoospermia in monkeys and rats via germ cell apoptosis. Local warming of monkey testes in water at 43°C for 2 consecutive days (30 min per day) decreased the number of sperm in the semen by up to 80% on d 28, and the effect was completely reversed on d 144. Germ cells rely heavily on Sertoli cells for structural and nutritional support. Specialized junctions that play a pivotal role in spermatogenesis occur at sites of Sertoli-Sertoli and Sertoli-germ cell contact in the seminiferous epithelium. We demonstrated that expression of tight junction (TJ)-associated molecules, such as occludin and zonula occludens-1 (ZO-1), were greatly reduced 24–48 h after heat treatment, while the permeability of the blood-testis barrier (BTB) was simultaneously increased, but recovered 10 d later. These results indicate a reversible disruption of the BTB associated with transient inductions of transforming growth factor (TGF) β2 and β3 expression, p38 mitogen-activated protein kinase and extracellular signal-regulated kinase activation, and concomitant loss of occludin and ZO-1. This suggests that expression of TJ-associated molecules and the BTB was reversibly perturbed by mild testicular hyperthermia, and that the heat-induced induction of TGF-β might be involved in downregulating TJ-associated proteins, leading to cell junction reduction. This review discusses the changes in total gene expression patterns after experimental cryptorchidism in adult mouse testes, and the cloning of several novel, physiologically significant spermatogenesis-specific genes.

关键词： cryptorchidism spermatogenesis apoptosis heat stress Sertoli cells blood-testis barrier tight junction

Role of ubiquitination in meiotic recombination repair

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Science china(Life sciences) 2010年第4期53卷 447-454页

作者： CUI Li & LI Wei state key laboratory of Reproductive Biology, institute of zoology, chinese academy of sciences, beijing 100101, china state key laboratory of Reproductive Biology Institute of Zoology Chinese Academy of Sciences Beijing China

Programmed and unprogrammed double-strand breaks (DSBs) often arise from such physiological requirements as meiotic recombination, and exogenous insults, such as ionizing radiation (IR). Due to deleterious impacts on genome stability, DSBs must be appropriately processed and repaired in a regulatory manner. Recent investigations have indicated that ubiquitination is a critical factor in DNA damage response and meiotic recombination repair. This review summarizes the effects of proteins and complexes associated with ubiquitination with regard to homologous recombination (HR)-dependent DSB repair.

关键词： ubiquitin meiosis homologous recombination DNA double-strand breaks

A new trust region algorithm for image restoration

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Science china Mathematics 2005年第2期48卷 169-184页

作者： WEN Zaiwen & WANG Yanfei state key laboratory of Scientific engineering Computing, institute of Computational Mathematics and Scientific/engineering Computing, academy of Mathematics and System sciences, chinese academy of sciences, beijing 100080, china National key laboratory on Remote Sensing Science, institute of Remote Sensing Applications, chinese academy of sciences, beijing 100101, china state key laboratory of Scientific engineering Computing Institute of Computational Mathematics and Scientific/Engineering Computing Academy of Mathematics and System Sciences Chinese Academy of Sciences Beijing China National key laboratory on Remote Sensing Science Institute of Remote Sensing Applications Chinese Academy of Sciences Beijing China

The image restoration problems play an important role in remote sensing and astronomical image analysis. One common method for the recovery of a true image from corrupted or blurred image is the least squares error (LSE) method. But the LSE method is unstable in practical applications. A popular way to overcome instability is the Tikhonov regularization. However, difficulties will encounter when adjusting the so-called regularization parameter a. Moreover, how to truncate the iteration at appropriate steps is also challenging. In this paper we use the trust region method to deal with the image restoration problem, meanwhile, the trust region subproblem is solved by the truncated Lanczos method and the preconditioned truncated Lanczos method. We also develop a fast algorithm for evaluating the Kronecker matrix-vector product when the matrix is banded. The trust region method is very stable and robust, and it has the nice property of updating the trust region automatically. This releases us from tedious finding the regularization parameters and truncation levels. Some numerical tests on remotely sensed images are given to show that the trust region method is promising.

关键词： trust region algorithm, image restoration, Lanczos method, Kronecker matrix-vector product, preconditioning.

SILICA-SUPPORTED NICKEL AND ZIRCONIUM CATALYSTS FOR BRANCHED POLYETHYLENE

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chinese Journal of Polymer Science 2003年第4期21卷 453-458页

作者：孙文华 state key laboratory of engineering Plastics The Center for Molecular sciences institute of Chemistry chinese academy of sciences beijing 100080 china Stt. key Lab. of Eng. Plastics Center for Molecular Sciences Chinese Academy of Sciences Beijing 100080 China

8-Aminoquinoline nickel dichloride and bis(cyclopentadienyl)zirconium dichloride (Cp_2ZrCl_2) were supportedsimultaneously on silica to produce branched polyethylene successfully by combined polymerization. The supportedpolymerization results showed that the molecular weight of polyethylene increased while the molecular weight distributionbecame wider and the molecular chains of oligomers remaning in the final solution became shorter as compared to theoligomers obtained in polymerization processes with pure 8-aminoquinoline nickel dichloride catalysis, as well as theCp_2ZrCl_2 and nickel combination system. With decreasing amount of Ni catalyst in the supported catalyst, the molecular chains of oligomers in the resulting solution became shorter, while α-olefin selectivity increased.

关键词： 8-Aminoquinoline nickel dichloride, Cp_2ZrCl_2 Silica supported catalysts Branched polyethylene

CORRECTION METHODS FOR STEADY INCOMPRESSIBLE FLOWS

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Journal of Computational Mathematics 1999年第4期17卷 419-424页

作者： Jian Li(state key laboratory of Scientific and engineering Computing, ICMSEC, chinese academy of sciences, beijing 100080, china) 中科院计算数学与科学工程计算所科学与工程计算国家重点实验室北京

Correction methods for the steady semi-periodic motion of incompressible fluid are investigated. The idea is similar to the influence matrix to solve the lack of vorticity boundary conditions. For any given boundary condition of the vorticity, the coupled vorticity-stream function formulation is solved. Then solve the governing equations with the correction boundary conditions to improve the solution. These equations are numerically solved by Fourier series truncation and finite difference method. The two numerical techniques are employed to treat the non-linear terms. The first method for small Reynolds number R equals 0-50 has the same results as that in M. Anwar and S.C.R. Dennis' report. The second one for R greater than 50 obtains the reliable results. (Author abstract) 4 Refs.

关键词： incompressible flow vorticity stream function numerical solution

NF-κB,a hot topic in biochemical and medical studies in china

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Science china(Life sciences) 2010年第12期53卷 1485-1487页

作者： WEI Yan state key laboratory of Brain and Cognitive sciences,institute of Biophysics,chinese academy of sciences,beijing 100101,china state key laboratory of Brain and Cognitive sciences Institute of Biophysics Chinese Academy of Sciences Beijing China

In 1986, nuclear factor (NF)-κB was first discovered in the laboratory of Nobel Prize laureate David Baltimore via its interaction with a sequence (5′-GGGACTTTCC-3′) in the immunoglobulin light-chain enhancer in B

关键词： NF-κB transcription factor signaling pathway upregulation downregulation

GM-CSF-induced Ca_(2+) -activated non-voltage-dependent inwardly rectifying K_+ channel in murine peritoneal exudate macrophages

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chinese Science Bulletin 1997年第11期42卷 947-952页

作者： ZHANG Xiaofeng, FENG Meifu, WU Caihong and ZHOU Pei’aistate key laboratory of biomembrane and membrane Biotechnology, chinese academy of sciences, beijing 100080, china 1 . In-stitute of zoology, chinese academy of sciences, beijing 100080, china 2 College of Life sciences, Peking University, beijing 100871, china state key laboratory of biomembrane and membrane Biotechnology Chinese Academy of Sciences Beijing China institute of zoology Chinese Academy of Sciences Beijing China College of Life sciences Peking University Beijing China

GRANULOCYTE-MACROPHAGE colony-stimulating factor(GM-CSF),a member in the family ofglycoprotein cytokines,can not only act to stimulate proliferation of immature progenitors,butalso enhance differentiated functions of mature effector *** has two kinds of biologicaleffects on mature effector cells,*** effect and indirect *** latter is also

关键词： K+ channel patch clamp GM-CSF macrophage.