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Molecular Systematic Studies on Chinese Mandarina silkworm (Bombyx mandarina M.) and Domestic silkworm (Bombyx mori L.)

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Agricultural Sciences in China 2002年第3期1卷 349-358页

作者： LU Cheng, YU Hong-shi and XIANG Zhong-huai( The Key sericultural Laboratory of Agricultural Ministry, College of silkworm and silk engineering , Southwest Agricultural University, Chongqing 400716) The Key sericultural Laboratory of Agricultural Ministry College of Silkworm and Silk engineering Southwest Agricultural University Chongqing 400716

Molecular systematic studies on mandarina silkworm (Bombyx mandarina M.) in 11 regions in China and 25 representative strains of domestic silkworm (Bombyx mori L.) were conducted using molecular biology techniques. Results obtained from the analysis of DNA polymorphism and clustering of all the silkworm samples provide new evidence for the view that the domestic silkworm originated from the Chinese mandarina silkworm. On the basis of literature reviewing, a new hypothesis on the origin of the domestic silkworm was put forward. It was thought that the domestic silkworm was most probably domesticated from the Chinese mandarina silkworm of different ecotypes including monovoltinism, bivoltinism and multivoltinism; and that the domestic silkworm had the genetic background of monovoltinism, bivoltinism and multivoltinism at the very beginning of the domestication. The current strains of the domestic silkworm of different voltinism are the evolutionary results of thousands of years of rearing and artificial selections.

关键词： Bombyx mandarina M. Bombyx mori L. DNA polymorphism Systematic evolution

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桑蚕膨松丝及其针织产品的开发研究

丝绸 1995年第11期32卷 43-45页

作者： Suzhou Institute of silk textiles Bulky Yarn Research Group

介绍了桑蚕膨松丝的主要性能，利用这种新形质生丝开发真丝针织物新品种及工艺条件，对其织物性能与风格进行了测试和分析，从测试结果说明桑蚕膨松丝是一种品质优良，特别适用于真丝针织的丝绸新材料。

关键词：桑蚕膨松丝针织物开发蚕丝

Structural Analysis of Fibroin Heavy Chain Signal Peptide of silkworm Bombyx mori

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Acta Biochimica et Biophysica Sinica 2006年第7期38卷 507-513页

作者： Sheng-Peng WANG~(1,2)Ting-Qing GUO~1 Xiu-Yang GUO~1 Jun-Ting HUANG~2 Chang-De LU~(1*) 1 Institute of Biochemistry and Cell Biology,Shanghai Institutes for Biological Sciences,Chinese Academy of Sciences,Shanghai 200031,China 2.sericultural Research Institute,Chinese Academy of Agricultural Sciences,Zhenjiang 212018,China InstituteofBiochemistryandCellBiology ShanghaiInstitutesforBiologicalSciencesChineseAcademyofSciencesShanghai200031China sericulturalResearchInstitute ChineseAcademyofAgriculturalSciencesZhenjiang212018China

To study the minimal length required for the secretion of recombinant proteins and silkproteins in posterior silk gland,the signal peptide(SP)of the fibroin heavy chain(FibH)of silkwormBombyx mori was systematically shortened from the *** effect on the secretion of protein wasobserved using enhanced green fluorescent protein(EGFP)as a *** of EGFP fusion proteinswas examined under fluorescence *** SPs with lengths of 20,18,16 and 12 *** directthe secretion of the reporter,yet those with lengths of 11, 10, 9, 8 and 1 a.a. can not. When the FibH SP wasshortened to 12a.a., the secretion efficiency was decreased slightly and cleavage occurred within *** *** the FibH SP were used,the secretion of fusion protein was normal and the cleavage sitewas between the Gly-Ser linker and Met,the starting amino acid of EGFP. These findings are applicable forthe expression of foreign proteins in silkworm silk *** cleavage site of the SP is discussed andcompared with the predictive results of the SignalP 3.0 online prediction program.

关键词： signal peptide fibroin heavy chain silkworm Bombyx mori rAcMNPV

The Properties of Native silk Fibroin (SF) Solution/Gel from Bombyx mori silkworms during the Full Fifth Instar Larval Stage

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Journal of Wuhan University of Technology(Materials Science) 2011年第2期26卷 263-269页

作者：王洪 Key Laboratory of Textile Science & Technology Ministry of EducationDonghua University

The properties of native silk fibroin （SF） solution in the gland of silkworms during the full fifth instar larval stage were examined in an attempt to elucidate the mechanism of natural silk spinning in the *** flow and gelation behavior,birefringence phenomenon,rheological properties,specific viscosity and conformation of SF solutions from the gland of silkworms were measured by polarized light microscope,HAKKE rheometer,Ubbelohde viscometer and Solid-state 13C NMR,*** comparing their properties with regenerated SF solutions from natural silk fibers,it is believed that there exists a progressive maturation process favorable to spin silk fibers with excellent properties from native SF solution and a weak bonded and highly oriented SF gel network with SF molecules partly extended in α-helix conformation is formed in the middle section of the gland of *** suggests that a biomimetic maturation process for making spinnable solution might be necessary for artificial silk fiber spinning in order to obtain improved fiber properties.

关键词： silk fibre spin viscosity sol/gel

PHOTOFADING MECHANISM OF ARYLMETHANE DYES ON REAL silk

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苏州大学学报（工科版） 1994年第S2期19卷 35-42页

作者： Shao Jianzhong, Xie Renzhang & Zlieng Xuming(Department of Textile Chemical Engineering Zhejiang Institute of silk textiles Hangzhou. Zhejiang. P. R.CHINA)

The effect of simulated silk compounds- amino acids and their yellowing substances onphotofading of ary1methane *** the influence of external factors such as *** wave band of light source and nickel salt on phototfading of the dyes on real silkare investigated. The results show that tryptophan and tyrosine well as their yellowing sub-stances accelerate photofading of arylmethane dyes;the photofading process of arylmethandyes on real silk is a photooxidation process and external factors exert different influences onit.

关键词： Real silk Ammo Acid Arylmethane Dye Photofading Yellowing External Factors

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紫草染料对真丝织物染色性能的研究

丝绸 2002年第5期39卷 22-24页

作者：周秋宝余志成陈莹浙江工程学院材料与纺织学院浙江杭州310033 中国丝绸博物馆浙江杭州310002

用乙醇提取天然染料紫草根 ,对提取条件、染色工艺进行了研究分析 ,并对不同工艺染色的真丝织物的各项牢度作了比较。

关键词：天然染料紫草根丝织物染色牢度

Studies on the Mutant Systems of the Bombyx mori Gene Bank

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Agricultural Sciences in China 2002年第11期1卷 1277-1285页

作者： LU Cheng, DAI Fang-yin and XIANG Zhong-huai(The Key sericultural Laboratory, Ministry of Agriculture/Southwest Agricultural University,Chongqing 400716, P.R. China) The Key sericultural Laboratory Ministry of Agriculture/Southwest Agricultural University Chongqing 400716 P.R.China

Through over ten years of study, more than 1 000 genetic materials including mutant genes, chromosomal variation strains and special genetic materials of Bombyx mori, Linnaeus, collected, introduced or created since 1940s especially late 1980s, have been sorted out and put in order. After identifications and genetic analyses of their morphological, physiological and biochemical characters, the silkworm gene bank was constructed and the preservation system was perfected, and more than 600 silkworm strains were kept in this gene bank. The preserved silkworm mutant genes have covered more than 90% of existent ones across the world, in which, more than 100 are rare and precious mutant genes, and over 60 mutant genes were found and studied for the first time. Through hybrid analyses, linkage tests and three-point gene location tests, a perfect linkage retrieval labeling system of silkworm was established, which included 230 marker genes covering all the 28 linkage groups of Bombyx mori. The gene location system (composite system of recessive genes) of different linkage groups was set up. The intergenic complementation of mutant egg color and third type of maternal heredity egg color have been found, and indicated that the epistatic effect of mutant gene of white egg is universal. Twenty eight independent near isogenic lines marked with morphological mutation gene have been created and a series of novel breeding materials possessing great potential for application such as high feeding efficiency, special sex markers, natural colored silk, resistance to disease, wider feeding range and adjustable parthenogenesis, etc. , have been developed. The sustainable maintenance and management technique system of silkworm gene resources were well established.

关键词： Bombyx mori Mutation Gene bank Genetic analysis Gene-resources development

DYEING CHARACTERISTICS OF CROSSLINKED silk FABRIC IN THE SWOLLEN STATE

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苏州大学学报（工科版） 1995年第S3期20卷 29-35页

作者： W.K.LEEandK.M.SIN(Instituteof textiles & ClothingHong Kong Polytechnic. Kowloon, Hong Kong)

In practice,dyeing of silk fabric is very sensitiver to undue mechanical action whichcauses fabric *** sensitivity has led indirectly to unlevel dyeing and uneven shading of dyed *** approach to alleviate this problem is to dye the fabric after a fin-ishing operation which provides resistance to fabric distortion and which enhances higherdye up- take .silk fabric,crosslinked in the swollen state,exhibits high wet crease recoveryas well as higher moisture regain than the untreated *** dual- property from thetreatment may provide a solution of unlevel dyeing of silk *** paper reports the dye-ing characteristics of a crepe silk fabric,crosslinked in the swollen state,with selected acid,cationic,disperse and reactive *** the exception of reactive dye,all the three other se-lected classes of dye produced a higher up- take on the silk fabric.A postulation of the spe-cific dye-site for reactive dye for silk is spresented.

关键词：出口

有机膨润土吸附硝基苯的性能及其在废水处理中的应用

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水处理技术 1994年第4期20卷 236-240页

作者：顾曼华沈小强沈学优朱利中浙江丝绸工学院杭州大学环境科学系

分别用氯化十六烷基吡啶（ＣＰＣ）和溴化十六烷基三甲铵（ＣＴＭＡＢ）改性膨润土。研究了有机膨润土吸附水中硝基苯的性能及其影响因素。２５℃时，ＣＴＭＡＢ—膨润土和ＣＰＣ—膨润土的饱和吸附容量分别为１１７．０ｍｇ／ｇ和８７... 详细信息

分别用氯化十六烷基吡啶（ＣＰＣ）和溴化十六烷基三甲铵（ＣＴＭＡＢ）改性膨润土。研究了有机膨润土吸附水中硝基苯的性能及其影响因素。２５℃时，ＣＴＭＡＢ—膨润土和ＣＰＣ—膨润土的饱和吸附容量分别为１１７．０ｍｇ／ｇ和８７．６ｍｇ／ｇ。比原土（５８．１ｍｇ／ｇ）有较大的增加。有机膨润土对水中硝基苯的去除率为５０～６０％，比原土（０．５％）提高了４～５倍将Ａｌ２（ＳＯ４）３与有机膨润土联用处理废水，不但能提高硝基苯的去除率，还能大大降低废水的ＣＯＤＣｒ。

关键词：膨润土硝基苯吸附废水处理 CODCr