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Insulin-sensitizing effects of a novel α-methyl-α-phenoxylpropionate derivative in vitro

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Acta Pharmacologica Sinica 2007年第3期28卷 417-422页

作者： Hao-shu WU~(2.4) Juan-hong YU~(3,4) Ying-yin LI~2.Yu-she YANG~3 Qiao-jun HE~2.Yi-jia LOU~(2.5) Ru-yun JI~3 ~2.epartment of Pharmacology and Toxicology,College of Pharmaceutical sciences,Zhejiang University,Hangzhou 310058,china ~3State Key Laboratory of Drug Research,{2. Institute of Materia Medica,{2. Institutes for Biological sciences,Graduate school of the Chinese Academy of sciences,{2. 2.12.3,china Department of Pharmacology and Toxicology College of Pharmaceutical Sciences Zhejiang University Hangzhou 310058 China Department of State Key Laboratory of Drug Research Shanghai Institute of Materia Medica Shanghai Institutes for Biological Sciences Graduate School of the Chinese Academy of Sciences Shanghai 201203 China

Aim:To examine the insulin sensitizing effects of a novel α-methyl-α-phenoxylpropionate derivative YY2. in insulin-sensitive cell ***:Theperoxisome proliferator-activated receptor γ（PPARγ）agonist bioactivities of YY2.were detected by a preadipocyte differentiation ***-PCR and Westernblotting analysis were used to detect the expression of the target gene or *** effects of YY2. on insulin-mediated glucose consumption were determined inthe HepG2.human hepatocellular carcinoma ***:YY2. could enhancethe differentiation of preadipocytes to adipocytes and upregulate the gene ex-pression of PPARγ2.as well as the protein expression of insulin receptor sub-strate-1（IRS-1）,glucose transporter-4（GLUT4）,and adiponectin（ACRP30）.Theeffects on GLUT4 and ACRP30 could be reversed by the PPARγ inhibitor ***,YY2. efficiently reduced glucose consumptions in HepG2.cells after2. h culture,and the effects were related to insulin and YY2. ***:YY2.,a potential insulin-sensitizing agent like rosiglitazone,couldenhance glucose consumption in HepG2.cells in a concentration-and insulin-dependent *** may improve the insulin resistance associated with type 2.iabetes.

关键词： YY2. peroxisome proliferator-activated receptor y insulin sensitivity glucose consumption

Hydrothermal route to Eu doped LuO(OH) and Lu_2._3 nanorods

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Science china(Technological sciences) 2010年第6期53卷 1576-1582页

作者： QIU HuaJun1,SHI Ying1,XIE JianJun1,XIE Jie1,ZHANG LinLin2.& XU FangFang2.1 school of Materials Science and Engineering,shanghai University,shanghai 2.0072.china 2.shanghai Institute of Ceramics,Chinese Academy of sciences,shanghai 2.0050,china 1. school of Materials Science and Engineering Shanghai University Shanghai 200072 China2. Shanghai Institute of Ceramics Chinese Academy of Sciences Shanghai 200050 China

A facile method was developed to synthesize Eu doped LuO(OH) nanorods through hydrothermal processing and Lu2.3 nanorods by subsequent *** microstructural morphologies of the Lu-based nanostructures could be controlled by simply varying the concentration of NaOH in hydrothermal processing as *** observation revealed that the obtained LuO(OH) nanorods after hydrothermal processing had a uniform diameter of 10-2. nm and a length around 100 *** heat treatment at 600-700°C for 2.h,the high length/diameter ratio was sustained in the obtained Lu2.3 nanorods with different sizes depending on the calcining temperatures.

关键词： nanorods hydrothermal processing LuO(OH) Lu2.3

Preparation and characterization of some surface negatively charged residue mutants of cytochrome b_5

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Chinese Science Bulletin 2001年第7期46卷 555-559页

作者： WANG Yunhua, WANG Wenhu, LU Junxia, REN Yi, GU Shaohua, XIE Yi & HUANG Zhongxian1. Chemical Biology Lab, Department of Chemistry, fudan University, shanghai 2.0433, {2. 2. Genetic Institute, school of Life, fudan University, shanghai 2.0433, {2. Chemical Biology Lab Department of Chemistry Fudan University Shanghai China Genetic Institute School of Life Fudan University Shanghai China

Site-directed mutagenesis was used to obtain seven variants of tryptic fragment of bovine liver cytochrome bs (cyt bs), in which the negatively charged residues around the heme exposed edge of cyt bs were replaced by hydropho-bic amino acid alanine. Double-site mutants, triple-site mutants and even quadruple-site mutants were obtained. DNA sequencing and molecular weight measurements of the mutant proteins both confirmed that these site-directed muta-genesises were successfully performed. Spectroelectrochem-istry of these mutant proteins revealed that the apparent redox potentials of these mutant proteins caused a positive shift of 2.10 mV. The global structure of these mutant proteins did not show much difference from that of the wild type cyt bs, providing a solid base for the further study on the roles of the proteins’ surface charges.

关键词： cytochrome bs site-directed mutagenesis negative charge on the protein surface redox potential.

Effect of Pozzolanic Reaction Products on Alkali-silica Reaction

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Journal of Wuhan University of Technology(Materials Science) 2006年第3期21卷 168-171页

作者：魏风艳 shanghai K. Wah Concrete Co.Ltd Shanghai 200030China

The effect of fly ash on controlling alkail-silica rection （ASR） in simudated alkali solution was studied. The expausion of mortar bars and the content of Ca（ OH）2.in cement paste cured at 80 °G for 91 d were measured. Traasmission electron microscopy （TEM） and high-resolution transmission electron microscot9 （HRTEM） were employed to study the microstructure of C-S-H. TEM/ energy dispersive spectroscopy （EDS） leas then used to determine the composition of C-S-H. The pore structure of the paste was analyzed by mercury intntsion porosimetry （MIP）. The results show that the contents of fly ash of 30% and 45% can well inhibit ASR. And the content of Ca（OH） 2.decreases with the increase of fly ash. That fly ash reacted with Ca（OH）2.to produce C-S-H with a low Ca/Si molar ratio could bind more Na^＋ and K^＋ ious, and produce a reduction in the amount of soluble alkali available for ASR. At the same time, the C- S- H produced by pozzolanic reaction converted large pores to snudler ones （ gel pores smaller than 10 nm ） to deusify the pore structure. Perhaps that could inhibit alkali trausport to aggregate for ASR.

关键词： alkali Ca/ Si molar ratio C-S-H alkali-silica reaction

Identification and Characterization of a Rat Novel Gene RSEP4 Expressed Specifically in Central Nervous System

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Acta Biochimica et Biophysica Sinica 2004年第7期36卷 501-507页

作者： Xi-Dao WANG1,2. Ling-Wei KONG1, Zhi-Qin XIE1, Yu-Qiu ZHANG3, Zhi-Xin LIN2. Zhi-Qi ZHAO3,Lei YU4, and Nai-He JING1* 1 Laboratory of Molecular Cell Biology, Laboratory of Stem Cell Biology, Institute of Biochemistry and Cell Biology, shanghai Institutes for Biological sciences, Chinese Academy of sciences, Graduate school of the Chinese Academy of sciences, shanghai 2.0031, china school of Life Science and Biotechnology, 2. shanghai Jiaotong University, shanghai 2.0030, china Institute of Neurobiology, fudan University, {12., china 3 4 Department of Cell Biology, Neurobiology and Anatomy, College of Medicine, University of Cincinnati, Cincinnati, Ohio, USA Laboratory of Molecular Cell Biology and Stem Cell Biology Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Graduate School of the Chinese Academy of Sciences Shanghai China

The low-abundantly expressed genes composed the majorities of the mRNAs expressed in the central nervous system (CNS), and were thought to be important for the normal brain functions. Through differential screening a low-abundance cDNA sublibrary with mRNA from neuropathic pain of chronic constriction injury (CCI) model, we have identified a novel rat gene, rat spinal-cord expression protein 4 gene (RSEP4). The total length of RSEP4 cDNA is 2.06 bp, with a 501 nucleotide open reading frame (ORF) that encodes a 167 amino acid polypeptide. Northern blot revealed that RSEP4 was expressed specifically in the CNS. In situ hybridization showed that the mRNA of RSEP4 was strongly expressed in the CA1, CA2. CA3 and DG regions of hippocampus, the Purkinje cells of cerebellum, and the small sensory neurons of dorsal horn and large motor neurons of ventral horn of spinal cord. Over-expression of RSEP4-EGFP fusion protein in the human embryonic kidney 2.3T cells showed that RSEP4 protein was mainly localized in the cell cytoplasm. These results suggest that RSEP4 may play some roles in the CNS.

关键词： central nervous system (CNS) rat Northern blot in situ hybridization

Steady-state responses of axially accelerating viscoelastic beams: Approximate analysis and numerical confirmation

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Science china(Physics,Mechanics & Astronomy) 2008年第11期51卷 1707-1721页

作者： CHEN LiQun1,2.& DING Hu2.1 Department of Mechanics, shanghai University, shanghai 2.0444, {2. 2.shanghai Institute of Applied Mathematics and Mechanics, shanghai 2.0070, {2. Department of Mechanics Shanghai University Shanghai 200444 China shanghai Institute of Applied Mathematics and Mechanics Shanghai 200070 China

Nonlinear parametric vibration of axially accelerating viscoelastic beams is inves-tigated via an approximate analytical method with numerical confirmations. Based on nonlinear models of a finite-small-stretching slender beam moving at a speed with a periodic fluctuation, a solvability condition is established via the method of multiple scales for subharmonic resonance. Therefore, the amplitudes of steady-state periodic responses and their existence conditions are derived. The amplitudes of stable steady-state responses increase with the amplitude of the axial speed fluctuation, and decrease with the viscosity coefficient and the nonlinear coefficient. The minimum of the detuning parameter which causes the existence of a stable steady-state periodic response decreases with the amplitude of the axial speed fluctuation, and increases with the viscosity coefficient. Nu-merical solutions are sought via the finite difference scheme for a nonlinear par-tial-differential equation and a nonlinear integro-partial-differential equation. The calculation results qualitatively confirm the effects of the related parameters pre-dicted by the approximate analysis on the amplitude and the existence condition of the stable steady-state periodic responses. Quantitative comparisons demonstrate that the approximate analysis results have rather high precision.

关键词： nonlinearity parametric vibration axially accelerating beam method of multiple scales numerical confir-mation

Locating the Polar Cap Boundary of Postnoon Sector from Observations of 630.0 nm Auroral Emission at Zhongshan Station

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Wuhan University Journal of Natural sciences 2002年第1期7卷 59-64页

作者： Liu Li-gang 1, Ai Yong 1, Liu Rui-yuan 2. Yang Hui-gen 2.1. school of Electronic Information, Wuhan University, Wuhan 430072. Hubei, china 2. Polar Research Institute of china, shanghai 2.012., china Wuhan University School of Electronic Information Wuhan Hubei China (GRID:grid.49470.3e) (ISNI:***) Polar Research Institute of china Shanghai China (GRID:grid.418683.0) (ISNI:***)

We studied the ground observations of 630.0 nm auroral emission at Zhongshan Station to determine the polar cap boundary with the latitudinal profile of emission intensity. The open-closed field time boundary is assumed to lie at the boundary between polar rain and plasma sheet precipitation. We assume that nonprecipitation-dependent sources of 630.0 nm emission cause a spatially uniform luminosity in the polar cap and that auroral zone luminosity is also spatially uniform. Therefore we determine the location of the polar cap boundary of postnoon sector from the auroral emission data each time by finding the best fit of the observations to a step function in latitude and we produce a time series of the location of the polar cap boundary. The average error of the practice in the paper is less than 0.8 degree.

关键词： polar cap boundary aurora Zhongshan Station

Leukemia,an effective model for chemical biology and target therapy

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Acta Pharmacologica Sinica 2007年第9期28卷 1316-1324页

作者： Guo-qiang CHEN~(2.3,4),Li-shun WANG~2.Ying-li WU~2.Yun YU~2.2.Department of Pathophysiology,Key Laboratory of Cell Differentiation and Apoptosis of Chinese Ministry of Education,shanghai Jiao TongUniversity school of Medicine(SJTU-SM,formerly shanghai Second Medical University),shanghai 2.002.,china,~3 Institute of HealthScience,SJTU-SM/shanghai Institutes for Biological sciences,shanghai 2.002.,china Department of Pathophysiology Key Laboratory of Cell Differentiation and Apoptosis of Chinese Ministry of Education Shanghai Jiao Tong University School of Medicine (SJTU-SM formerly Shanghai Second Medical University) Shanghai China Institute of Health Science SJTU-SM/Shanghai Institutes for Biological Sciences Shanghai China

The rapid rise of chemical biology aimed at studying signaling networks for basiccellular activities using specific,active small molecules as probes has greatlyaccelerated research on pathological mechanisms and target therapy of *** research is especially important for malignant tumors such as leukemia,aheterogeneous group of hematopoietic malignancies that occurs *** use of a chemical approach combined with genetic manipulation,great progresshas been achieved over the past few decades on the biological,molecular andcytogenetic aspects of leukemia,and in its diagnosis and *** particular,discoveries of the clinical effectiveness of all-trans retinoic acid and arsenictrioxide in the treatment of acute promyelocytic leukemia and the kinase inhibitorsImatinib and Dasatinib in the treatment of chronic myelogenous leukemia not onlymake target therapy of leukemia a reality,but also push mechanisms of leukemo-genesis and leukemic cell activities *** review will outline advances inchemical biology that help our understanding of the molecular mechanisms of celldifferentiation and apoptosis induction and target therapy of leukemia.

关键词： leukemia chemical biology target therapy retinoic acid arsenic trioxide differentia tion apoptosis

Weierstrass Representation for Surfaces in the Three-Dimensional Heisenberg Group

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Chinese Annals of Mathematics,Series B 2010年第1期31卷 119-132页

作者： Qun CHEN Hongbing QIU school of Mathematics and Statistics Wuhan University Wuhan 430072 China Corresponding author. school of mathematical {2. Fudan University Shanghai 200433 China

The authors define the Gauss map of surfaces in the three-dimensional Heisenberg group and give a representation formula for surfaces of prescribed mean ***,a second order partial differential equation for the Gauss m... 详细信息

关键词： Heisenberg group Mean curvature Weierstrass representation