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Science china Chemistry 2011年第6期54卷 951-956页

作者： WU Hui, JIN Can, HUANG GuoLi, WANG LianJun, JIANG JuLi & WANG LeYong key laboratory of Mesoscopic Chemistry of ministry of education School of Chemistry and chemical Engineering, Nanjing University, Nanjing 210093, china key laboratory of Mesoscopic Chemistry of ministry of education School of Chemistry and chemical Engineering Nanjing University Nanjing China

Two new bis-imidazolinium salts (4a, 4b) have been synthesized as precursors of N-heterocyclic carbenes (NHCs) from the commercially available (R)-2,2′-dihydroxy-1,1′-binaphthalene. The two bis-imidazolinium salts were used as efficient precursor of NHC ancillary ligands in the palladium-catalyzed Heck reaction. Good to excellent yields and high stereoselectivities were obtained with ethyl acrylate, acrylonitrile, and acrylamide as starting materials. The structure of bis-imidazolinium salt 4b was further characterized by single crystal X-ray diffraction analysis.

关键词： N-heterocyclic carbenes Heck reaction palladium imidazolinium salts

Aptamer-based competitive electrochemical assay of small biomolecules

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Science china Chemistry 2011年第5期54卷 822-826页

作者： ZHANG YuYong,XIANG Yun,CHAI YaQin & YUAN Ruo key laboratory on Luminescence and Real-Time Analysis,ministry of education School of Chemistry and chemical Engineering,Southwest University,Chongqing 400715,china key laboratory on Luminescence and Real-Time Analysis Ministry of Education School of Chemistry and chemical Engineering Southwest University Chongqing China

A convenient aptamer-based competitive electrochemical biosensor for a small biomolecule,adenosine,was described. The sensing surface was fabricated by self-assembly of an aptamer/mercaptohexanol monolayer on a gold disk electrode. The principle of this aptasensor is based on the competition between an adenosine target molecule and a ferrocene-conjugated signaling DNA strand for the aptamer binding site on the sensing surface. Due to the competitive nature of this assay,the electrochemical responses of the surface captured ferrocene are inversely proportional to log[adenosine] in the range from 0.05 to 3.2 μM,with a detection limit of 25 nM. Moreover,the aptasensor also shows high selectivity for adenosine. The proposed aptasensor thus holds great potential for the detection of other small biomolecules.

关键词： aptamer adenosine biosensor competitive assay differential pulse voltammetry

Efficient expression of codon-adapted human acetaldehyde dehydrogenase 2 cDNA with 6×His tag in Pichia pastoris

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Science china(Life Sciences) 2009年第10期52卷 935-941页

作者： ZHAO YuFeng1,2, LEI MingKe2, WU YuanXin2, ZHANG ZiSheng3 & WANG CunWen2 1 State key laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agriculture University, wuhan 430070, china 2.key laboratory for Green chemical Processes of the ministry of education, Hubei key Lab of Novel Reactor & Green chemical Technology, School of chemical Engineering and Pharmacy, wuhan Institute of Technology, wuhan 430074, china 3 Department of chemical Engineering, University of Ottawa, Ottawa, Canada K1N 6N5 State key laboratory of Agricultural Microbiology College of Life Science and Technology Huazhong Agriculture University Wuhan China key laboratory for Green chemical Processes of the ministry of education Hubei Key Lab of Novel Reactor & Green Chemical Technology School of Chemical Engineering and Pharmacy Wuhan Institute of Technology Wuhan China Department of chemical Engineering University of Ottawa Ottawa Canada

Human mitochondrial acetaldehyde dehydrogenase 2 (ALDH2) catalyzes the oxidation of acetaldehyde to acetic acid. Therefore, ALDH2 has therapeutic potential in detoxification of acetaldehyde. Further-more, ALDH2 catalyzes nitroglycerin to nitrate and 1, 2-glyceryldinitrate during therapy for angina pectoris, myocardial infarction, and heart failure. Large quantities of ALDH2 will be needed for potential clinical practice. In this study, Pichia pastoris was used as a platform for expression of human ALDH2. Based on the ALDH2*1 cDNA sequence, we designed ALDH2 cDNA by choosing the P. pastoris preferred codons and by decreasing the G + C content level. The sequence was synthesized using the overlap extension PCR method. The cDNA and 6×His tags were subcloned into the plasmid pPIC9K. The recombinant protein was expressed in P. pastoris GS115 and purified using Ni2+-Sepharose affinity chromatography. The amount of secreted protein in the culture was 80 mg/L in shake-flask cultivation and 260 mg/L in high-density bioreactor fermentation. Secreted ALDH2 was easily purified from the culture supernatant by using Ni2+-Sepharose affinity chromatography. After purification of the fermentation supernatant, the enzyme had a specific activity of 1.2 U/mg protein. The yield was about 16 mg/L in a shake flask culture of P. pastoris GS115 which contained the original human ALDH2*1 cDNA.

关键词： acetaldehyde dehydrogenase codon optimization overlap extension PCR Pichia pastoris.

Application of L-thiazolidine-4-carboxylic acid monolayer in electrochemical determination of copper(Ⅱ)

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Science china Chemistry 2010年第1期53卷 257-262页

作者： CUI Xin1,FU YingZi1,2,LI MiaoSi1,CHEN Min2,HE Xuan1,LIU XiaoHua1 & FENG XiaoMing1 1key laboratory of Green Chemistry & Technology,ministry of education College of Chemistry,Sichuan University,Chengdu 610064,china 2key laboratory of Luminescence and Real-Time Analysis,ministry of education College of Chemistry and chemical Engineering,Southwest University,Chongqing 400715,china key laboratory of Green Chemistry & Technology Ministry of Education College of Chemistry Sichuan University Chengdu China key laboratory of Luminescence and Real-Time Analysis Ministry of Education College of Chemistry and chemical Engineering Southwest University Chongqing China

L-Thiazolidine-4-carboxylic acid monolayer was prepared on gold electrodes through the self-assembly *** novel thioether-based monolayer could efficiently preconcentrate Cu2+,which provided a simple,stable and reproducible method for the determination of Cu2+.The modified electrodes were stable enough to be continuously used for one week(more than 30 times regeneration) with lower than 10% decrease in the *** retained their initial activity for more than one month if used once a *** calibration curve was linear for Cu2+ from 0.6 to 158.8 μg L?1 with a detection limit of 0.38 μg L?*** relative standard deviation was 3.2% for a series of six successive *** proposed method was applied in the determination of Cu2+ in mineral water and human hair samples.

关键词： L-thiazolidine-4-carboxylic acid copper ions gold electrode thioether monolayer

Comparison of the affinity between acetylcholinesterase and small molecular inhibitors by Surface plasmon resonance using gold nanoparticles for signal enhancement

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Comparison of the affinity between acetylcholinesterase and ...

A Novel Artificial Synthetic Receptor for Hydroquinone

中国化学会第十四届有机分析及生物分析学术研讨会

作者： HUANG Xi TU Hai-yang DU Dan ZHANG Ai-dong~* (key laboratory of pesticide & chemical biology of ministry of education,Central china Normal University,wuhan 430079)

＜正＞1 Introduction The identification of high sensitive inhibitors to a target is crucial in the pesticide discovery *** is therefore essential to obtained sufficient informations on affinity and kinetic aspects of an interaction between inhibitors and their *** Plasmon Resonance （SPR）are essential tools in generating high-quality data on the interaction between target protein and aim com- *** have first-time introduced gold nanoparticles

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A Novel Artificial Synthetic Receptor for Hydroquinone

Integration and Optimized Utilization of Naphtha Resources

中国化学会第四届有机化学学术会议

作者： ZHOU, Bao-Han CHEN, Yun-Feng WU, An-Xin (key laboratory of pesticide & chemical biology, ministry of education, Department of Chemistry, Central china Normal University, wuhan 430079)

＜正＞The design and synthesis of host molecules for neutral guests continues to be an area of great interest in su-pramolecular chemistry. In recent years a series of receptors derived from the concave molecule glycoluril have been developed . These receptors, which are U-shaped, mostly bind resorcinol by hydrogen bonding and byπ-πstacking. Here we describe the binding properties of a new type of related molecular clips 1 derived from glycoluril. Binding hydroquinone 2 by hydrogen bonding interactions between the hydroxy groups of the guest and the urea carbonyl groups of the host and by π -πstacking interactions between the guest and the host sidewalls.

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china Petroleum Processing & Petrochemical Technology 2010年第2期12卷 51-56页

作者： Li Yajun Zhang Tingting (key laboratory of Enhanced Heat Transfer and Energy Conservation,ministry of education,School of chemical and chemical Engineering,South china University of Technology,Guangzhou 510640) key laboratory of Enhanced Heat Transfer and Energy Conservation Ministry of Education South China University of Technology Guangzhou 510640 China

Naphtha is an important raw material for manufacture of clean fuels and ethylene products. However, china is experiencing a serious imbalance between supply and demand of naphtha, due to its rapidly increasing car population and booming ethylene industry, the demand of which cannot be met by the domestic depleting crude oil resources. Focusing on alleviating the above-mentioned naphtha deficit, this paper puts forward an idea suggesting that china＇s limited naphtha resource should be used reasonably. Naphtha feedstocks with more potential aromatic content should be used in catalytic reforming process to produce clean fuel products, and those feedstocks with more paraffinic content should be used in ethylene production. Meanwhile, industry tests show that the low-valued naphtha byproduct from ethylene plants and the products of secondary processing units at refineries can also be applied so as to extend the naphtha supply for manufacture of cleaner fuels and ethylene derivatives.

关键词： cleaner gasoline ethylene, naphtha feedstock optimization

Tripod molecules build molecular networks and open-dimer capsules by weak interactions

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Science china Chemistry 2010年第4期53卷 851-857页

作者： HAN Jun1, DENG Chao1, FANG Ru1, LI YiZhi2, WANG LeYong1& PAN Yi2 1key laboratory of Mesoscopic Chemistry, ministry of education School of Chemistry and chemical Engineering, Nanjing University, Nanjing 210093, china 2State key laboratory of Coordination Chemistry, Nanjing University, Nanjing 210093, china key laboratory of Mesoscopic Chemistry Ministry of Education Nanjing University Nanjing China School of Chemistry and chemical Engineering Nanjing University Nanjing China State key laboratory of Coordination Chemistry Nanjing University Nanjing China

Three tripod molecules, tris(2-methoxy-5-nitrobenzyl)phosphine oxide (1), tris(2-butoxy-3-methyl-5-nitrobenzyl)phosphine oxide (2), and tris(3-nitrobenzyl)amine (TNBA), were synthesized and crystallized. The structures of 1, 2, and their comparison (TNBA) were determined by X-ray crystallography. It is noteworthy that compound 1 interacted with adjacent molecules via π-π stacking and C-H···π interactions to yield an open supramolecular network with the porosity P in 8.9%, whereas compound 2 gathered closely to form an open-dimer capsule by sixfold N-O···π and triple C-H···O interactions, which showed a rare example of a stable in, out-invertomer of phosphine inversion existing in open-dimers. A series of columns were built and arranged side by side by these weak interactions. By contrast, TNBA crystallized to form a 2D network maintained by C-H···O and C-H···πinteractions. It seems minor changes of the chemical structure may cause large differences in the crystal structure and interactions in crystal engineering.

关键词： tripod molecule supramolecular network open-dimer capsule weak interaction

Preparation of ZrSiO_4 Nano-powders by Micro-wave Hydrothermal Process

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稀有金属材料与工程 2011年第S3期40卷 202-205页

作者： Liu Dawei, Wang Feng, Zhu Jianfeng, Li Dong key laboratory of Auxiliary Chemistry & Technology for chemical Industry, ministry of education, Shaanxi University of Science and Technology, Xi’an 710021, china Shaanxi Univ Sci & Technol Key Lab Auxiliary Chem & Technol Chem Ind Minist Educ Xian 710021 Peoples R China

High purity ZrSiO4 powders were prepared using zirconium oxychloride and tetraethoxysilane as raw materials by the microwave-hydrothermal method at 160-200 ℃ for 30 min, in which NaF was added as mineralizer to promote the crystallization of zircon. The phase composition and microstructure of synthesized ZrSiO4 powders were investigated in detail. The results show that: the microwave-hydrothermal method greatly reduces the synthesis temperature of ZrSiO4 to 160 ℃, 30 min. The synthesized ZrSiO4 powders have small particle size and high stability up to 950 ℃. The synthesis mechanism of ZrSiO4 by microwave hydrothermal s process was also discussed.

关键词： microwave-hydrothermal method ZrSiO4 powders reaction mechanism

Engineering of drug nanoparticles by HGCP for pharmaceutical applications

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Particuology 2008年第4期6卷 239-251页

作者： Ting-Ting Hua, Jie-Xin Wanga, Zhi-Gang Shena, Jian-Feng Chena,b, a key Lab for Nanomaterials, ministry of education, Beijing University of chemical Technology, Beijing 100029, china b Research Center of ministry of education for High Gravity Engineering and Technology, Beijing University of chemical Technology, Beijing 100029, china key Lab for Nanomaterials Ministry of Education Beijing University of Chemical Technology Beijing 100029 China Research Center of ministry of education for High Gravity Engineering and Technology Beijing University of Chemical Technology Beijing 100029 China

This paper reviews our work on the fundamental principles of high gravity controlled precipitation (HGCP) technology, and its applications in the production of drug nanoparticles, which was carried out in a rotating packed bed (RPB). Several kinds of drug nanoparticles with narrow particle size distributions (PSDs) were successfully prepared via HGCP, including the 300-nm Cefuroxime Axetil (CFA) particles, 200–400-nm cephradine particles, 500-nm salbutamol sulfate (SS) particles (100 nm in width), and 850-nm beclomethasone dipropionate (BDP) particles, etc. Compared to drugs available in the current market, all the drug nanoparticles produced by HGCP exhibited advantages in both formulation and drug delivery, thus improving the bioavailability of drugs. HGCP is essentially a platform technology for the preparation of poorly water-soluble drug nanoparticles for oral and injection delivery, and of inhalable drugs for pulmonary delivery. Consequently, HGCP offers potential applications in the pharmaceutical industry due to its cost-effectiveness, efficient processing and the ease of scaling-up.

关键词： High gravity controlled precipitation Rotating packed bed Drug nanoparticle Poorly water-soluble drug Inhalable drug