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Rhodamine B doped silica nanoparticle labels for protein microarray detection

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Science china Chemistry 2010年第4期53卷 747-751页

作者： WANG Ying1, LI ZhongHui1, ZHONG WenYing2, LI Hui2, XU DanKe1 & CHEN HongYuan1 1key laboratory of Analytical Chemistry for Life Science, ministry of education School of Chemistry and chemical Engineering, Nanjing University, Nanjing 210093, china 2Department of Analytical Chemistry, china Pharmaceutical University, Nanjing 210009, china key laboratory of Analytical Chemistry for Life Science Ministry of Education School of Chemistry and chemical Engineering Nanjing University Nanjing China Department of Analytical Chemistry China Pharmaceutical University Nanjing China

A core-shell Rhodamine B-doped SiO2 nanoparticle was synthesized and its fluorescent intensity was found to be 1000 times higher than that of individual Rhodamine B molecule. The doped nanoparticles were further conjugated with streptavidin and the resulting nanoparticles were used in the detection of reverse-phase protein microarrays, in which human IgG of various concentrations was first immobilized on aldehyde-modified glass slides and then biotinlyated goat anti human IgG as well as the labeled nanoparticles were sequentially conjugated. The calibration curve is linear over the range from 800 fg to 500 pg and the limit of detection is 100 fg, which is 8 times lower than that of streptavidin-labeled Cy3 fluorescent dyes. The dyedoped SiO2 nanoparticles show potentials for the protein array detection.

关键词： Rhodamine B SiO2 nanoparticles protein detection protein microarrays

COMFA STUDY ON Α-（SUBSTITUTED PHENOXY ACETOXY） ALKYL PHOSPHONATES

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COMFA STUDY ON Α-（SUBSTITUTED PHENOXY ACETOXY） ALKYL PHOS...

Predicting bioconcentration factor values of organic pollutants based on medv descriptors derived QSARs

40th IUPAC Congress

作者： Song Yang Jian Wan* Ting Chen Hong-wu He*(key laboratory of pesticide & chemical biology ministry of china and College of Chemistry Central china Normal University Hubei wuhan 430079 china

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Science china Chemistry 2007年第5期50卷 587-592页

作者： CUI ShiHai1,2, YANG Jing1, LIU ShuShen2,3 & WANG LianSheng2 1 College of Chemistry and Environment, Nanjing Normal University, Nanjing 210097, china 2 State key laboratory of Pollution Control and Resources Reuse, School of the Environment, Nanjing University, Nanjing 210093, china 3 key laboratory of Yangtze Aquatic Environment, ministry of education, College of Environmental Science and Engineering, Tongji University, Shanghai 200092, china College of Chemistry and Environment Nanjing Normal UniversityNanjing 210097China 2 State key laboratory of Pollution Control and Resources Reuse School of the EnvironmentNanjing UniversityNanjing 210093China key laboratory of Yangtze Aquatic En

The molecular electronegativity-distance vector (MEDV) is employed todescribe the chemical structure of organic *** linear relationships between themolecular descriptors and BCF values are developed by best subset regression and partial leastsquare regression *** main structural factors influencing the bioactivities are -CH2,-X,-Cnot 2 and q2_(LOO) present good estimation ability and stabilityof *** prediction power for external samples is validated by the model developed from thetraining set.

关键词： POPs, BCF, MEDV, QSAR

Phenol Tert-Butylation Catalyzed by Zeolite H-Mordenite

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Transactions of Tianjin University 2005年第2期11卷 89-91页

作者：韩森李振花张悝 key laboratory for Green chemical Technology of education ministry of china School of Chemical Engineering and Technology Tianjin University Tianjin 300072 China

Para-tert-butyl phenol (p-TBP) and 2,4-di-tert-butyl phenol (2,4-DTBP) are widely used for the preparation of antioxidants. Zeolite catalysts showed good performance for the synthesis of p-TBP and 2,4-DTBP. In this work, zeolite H-mordenite (HM) catalyst was prepared and the alkylation of phenol with tert-butyl alcohol over zeolite HM catalyst was investigated at different reaction conditions. It is found that increasing temperature enhances the selectivity to p-TBP and the optimum reaction temperature for phenol conversion is 438 K. Increasing flow rate decreases phenol conversion apparently while the selectivity to p-TBP has a little increase. The suitable tert-butyl alcohol/phenol molar ratio is 2. Lower alcohol/phenol molar ratios are beneficial to p-TBP while higher ones are helpful for producing 2,4-DTBP.

关键词： phenol tert-butyl alcohol zeolite H-mordenite (HM) alkylation

Controlled Preparation of Monodisperse CoFe_2O_4 Nanoparticles by a Facile Method

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Journal of wuhan University of Technology(Materials Science) 2011年第2期26卷 258-262页

作者：王海 key laboratory of Fiber Optic Sensing Technology and Information Processing of ministry of education Wuhan University of Technology

CoFe2O4 nanoparticles （NPs） were synthesized by coprecipitation method using FeCl3·6H2O and CoCl2·6H2O as *** synthesized conditions were optimized,such as added means of precipitator,quantity of precipitator,the mol ratio of Fe 3＋ to Co2＋,reaction temperature and pH *** synthesized material was characterized by XRD,TEM,FTIR,EDS,Raman and its magnetic properties were studied by *** experimental results confirm that the sample is cubic spinel structure CoFe2O4 with a narrow size distribution and a good dispersion ***2O4 NPs with well-controlled shape and size was obtained at 70℃.The magnetic properties indicate superparamagnetic behavior and good saturated magnetization.

关键词： CoFe2O4 nanoparticles coprecipitation method monodispersity superparamagnetism

Expression,Purification and Characterization of the Phytopathogenic Fungi Ustilago maydis CYP51

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Expression,Purification and Characterization of the Phytopat...

Organic ammonium ion-occluded flexible coordination polymers:Thermal activation,structure transformation and proton transfer

2007中国科协年会

作者： Han Rui~1 Liu Deli~(1,2) Gao Qiang~1 Zhang Qingye~2 Wan Jian~2 Luo Qin~1 Xiao Wenjing~(2**) 1.College of life science,Central china Normal University 2.key laboratory of pesticide & chemical biology,Huazhong Untversity,ministry of education,wuhan 430079

Ustilago maydis is one of the most important pathogens of Corn Smut,causing the young corn orgonization of infection and formating the different size and shape of bacteria *** occurrence is more common,often leads to yield *** generated disease,can lead to serious yield loss,which is harmful to the people′s production and *** fungicide on the selection is ***51 is the target snzyme of fungicides and is a kind of transmembrane protein,sometimes it is difficult to express or the expression level is very ***:To investigate the relationship of with fungicides and to study the mechanism of drug resistance,recombinant *** CYP51(pET-UM-35)expressnd in *** was purified by Ni-NTA ***:The purified CYP51 exhibited one band in SDS-PAGE,and the molecular mass was about 66 *** reacted to triadimenol,it accquired the combination spectra by 5-3100.

关键词： Ustilago maydis CYP51 Expression and purification Characterization

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Science china Chemistry 2010年第10期53卷 2144-2151页

作者： XIE LinHua1, LIN JianBin1, LIU XiaoMin1, XUE Wei1, ZHANG WeiXiong1, LIU ShuXia2, ZHANG JiePeng1 & CHEN XiaoMing1 1MOE key laboratory of Bioinorganic and Synthetic Chemistry State key laboratory of Optoelectronic Materials and Technologies School of Chemistry and chemical Engineering, Sun Yat-Sen University, Guangzhou 510275, china 2key laboratory of Polyoxometalates Science of ministry of education College of Chemistry, Northeast Normal University, Changchun 130024, china MOE key laboratory of Bioinorganic and Synthetic Chemistry State key laboratory of Optoelectronic Materials and Technologies School of Chemistry and chemical Engineering Sun Yat-Sen University Guangzhou China key laboratory of Polyoxometalates Science of ministry of education College of Chemistry Northeast Normal University Changchun China

Solvothermal reactions of 1,3,5-benzenetricarboxylic acid (H3btc) with cadmium acetate or zinc acetate yielded two compounds formulated as (Me2NH2)[Cd(btc)]·DMA (1) (btc = 1,3,5-benzenetricarboxylate, DMA = N,N-dimethylacetamide) and (Me2NH2)[Zn(btc)]·DMF (2) (DMF = N,N-dimethylformamide). Both are 3-D frameworks with the rutile topology, which are constructed from six-connected dimeric metal cores and three-connected btc linkers. The solvent molecules and counter cations are located in the 1-D channels of the frameworks. A slight difference between the two compounds is the different connectivity modes of the metal atoms with the carboxylate groups of the ligands. However, this slight difference results in distinct flexibilities of the two frameworks. Variable-temperature powder X-ray diffraction studies revealed that the framework of 1 collapses when heated at 180 °C with loss of the guest species, but compound 2 undergoes two structural transformations below 380 °C. Thermogravimetry-infrared spectroscopy analysis for 2 showed that the two structural transformations are induced by separate losses of solvent molecules and counter cations, and that the dimethylammonium cations are eliminated as neutral dimethylamine molecules. IR spectroscopy demonstrated that the protons are transferred from the counter cations onto the uncoordinated carboxylate oxygen atoms on the channel walls. Sorption and proton conduction studies have also been performed for the compounds.

关键词： flexible coordination polymer proton transfer charged metal-organic framework rutile topology proton conduction

Preparation of micro-patterned surfaces of Si-N-O films and their influence on adhesion behavior of endothelial cells

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Science china(Technological Sciences) 2010年第1期53卷 257-263页

作者： ZHENG Nan,YANG Ping,WANG QiYi,YANG ZhongHai & HUANG Nan key laboratory of Advanced Technology for Materials of education ministry,key laboratory of Artificial Organ Surface Engineering of Sichuan,School of Materials Science and Engineering,Southwest Jiaotong University,Chengdu 610031,china key laboratory of Advanced Technology for Materials of education ministry Key Laboratory of Artificial Organ Surface Engineering of Sichuan School of Materials Science and Engineering Southwest Jiaotong University

Microgrooves were prepared on Si(100) surface by photolithography and wet ***,Si-N-O films were deposited on the microgrooves by unbalanced magnetron sputtering(UBMS) and micro-patterned surfaces of Si-N-O films were *** size of the micropatterns was measured by surface *** chemical composition of Si-N-O films were characterized by X-ray photoelectron spectrometry(XPS) and the wettability of the micropatterned surfaces was evaluated by contact angle *** behavior of endothelial cells adhered on the micro-patterned surfaces,including cells adhesion,proliferation and orientation,was evaluated by cell culture test and Alamar blue *** results showed that the amount and activity of endothelial cells on micro-patterned samples were higher than those on flat *** one day's incubation,the cells were well guided along the *** days later,most of the cells grew across the grooves and contacted each *** activity was also much higher than that on flat *** was suggested that the adhesion and proliferation of endothelial cells could be effectively enhanced by micropattern method.

关键词： micropatterns Si-N-O film endothelial cell unbalanced magnetron sputtering

An unexpected tetranuclear Li2Cr2 trimethylsilylacetylide complex bridged by two μ3-pyrazolates

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中国科学:化学 2012年第6期42卷 888-888页

作者： WANG Feng, CAO Li, LIAO HongLin, JIANG Jun, JIA XiaoLu, GAO Qian, LI DongFeng* key laboratory of pesticide and chemical biology of ministry of education College of Chemistry, Central china Normal University, wuhan 430079, china key laboratory of pesticide and chemical biology of ministry of education College of Chemistry Central china Normal University wuhan 430079 china

A tetranuclear Li2Cr2 acetylide precursor complex, [Li（Tp）CrIII（C≡CSiMe3）2（μ3-pz）]2·（n-pentane）2 (Tp=hydridotris（pyrazolyl）borate, pz = pyrazolate) （1） has been synthesized and characterized. The X-ray structure analysis shows that the complex contains a Li2Cr2 core bridged by two μ3-pyrazolates. The magnetic data exhibit the existence of weak antiferromagnetic interaction in the cluster.

关键词： acetylide complex synthesis X-ray structure magnetic properties