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Science china Chemistry 2009年第12期52卷 2071-2082页

作者： ZHU XiaoFei1 & TAN XiangShi1,2 1 Department of Chemistry,fudan university,shanghai 200433,china 2.institutes of biomedical sciences,fudan university,shanghai 200433,china 1. Department of Chemistry Fudan University Shanghai 200433 China 2. institutes of biomedical sciences Fudan University Shanghai 200433 China

This paper focuses on the group of metalloproteins/metalloenzymes in the acetyl-coenzyme A synthesis pathway of anaerobic microbes called Wood-Ljungdahl pathway,including formate dehydrogenase (FDH),corrinoid iron sulfur protein (CoFeSp),acetyl-CoA synthase (ACS) and CO dehydrogenase (CODH). FDH,a key metalloenzyme involved in the conversion of carbon dioxide to methyltetrahydrofolate,catalyzes the reversible oxidation of formate to carbon dioxide. CoFeSp,as a methyl group transformer,accepts the methyl group from CH3-H4 folate and then transfers it to ACS. CODH reversibly catalyzes the reduction of CO2 to CO and ACS functions for acetyl-coenzyme A synthesis through condensation of the methyl group,CO and coenzyme A,to finish the whole pathway. This paper introduces the structure,function and reaction mechanisms of these enzymes.

关键词： Wood-Ljungdahl pathway formate dehydrogenase corrinoid iron sulfur protein acetyl-CoA synthase CO dehydrogenase

recent developments of nanoparticle-based enrichment methods for mass spectrometric analysis in proteomics

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Science china Chemistry 2010年第4期53卷 695-703页

作者： ZHANG LiJuan, LU HaoJie & YANG pengYuan Department of Chemistry and institutes of biomedical sciences, fudan university, shanghai 200433, china 1. Department of Chemistry and institutes of biomedical sciences Fudan University Shanghai 200433 China

In proteome research, rapid and effective separation strategies are essential for successful protein identification due to the broad dynamic range of proteins in biological samples. Some important proteins are often expressed in ultra low abundance, thus making the pre-concentration procedure before mass spectrometric analysis prerequisite. The main purpose of enrichment is to isolate target molecules from complex mixtures to reduce sample complexity and facilitate the subsequent analyzing steps. The introduction of nanoparticles into this field has accelerated the development of enrichment methods. In this review, we mainly focus on recent developments of using different nanomaterials for pre-concentration of low-abundance peptides/ proteins, including those containing post-translational modifications, such as phosphorylation and glycosylation, prior to mass spectrometric analysis.

关键词： enrichment nanoparticles mass spectrometry proteomics phosphorylation glycosylation

A meta-analysis of oxidative stress markers in schizophrenia

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中国科学：生命科学英文版 2010年第1期53卷 112-124页

作者： ZHANG Ming1,2,ZHAO ZhongMing3,HE Lin1,2,4 & WAN ChunLing1,2 1 Bio-X Center,Key Laboratory for the Genetics of Developmental and Neuropsychiatric Disorders(Ministry of Education),shanghai Jiao Tong university,shanghai 200030,china 2.institutes for Nutritional sciences,shanghai Institute of Biological sciences,Chinese Academy of sciences,shanghai 200031,china 3 Departments of biomedical Informatics,psychiatry,and Cancer Biology,Vanderbilt university Medical Center,Nashville,TN 37232,USA 4 institutes of biomedical sciences,fudan university,shanghai 200032,china 1. Bio-X Center Key Laboratory for the Genetics of Developmental and Neuropsychiatric Disorders (Ministry of Education) Shanghai Jiao Tong University Shanghai 200030 China2. Institutes for Nutritional Sciences Shanghai Institute of Biological Sciences Chinese Academy of Sciences Shanghai 200031 China3. Departments of Biomedical Informatics Psychiatry and Cancer Biology Vanderbilt University Medical Center Nashville TN 37232 USA4. Institutes of Biomedical Sciences Fudan University Shanghai 200032 China

Oxidative stress has been identified as a possible element in the neuropathological processes of schizophrenia(SCZ).Alteration of oxidative stress markers has been reported in SCZ studies,but with inconsistent *** evaluate the risk of oxidative stress to schizophrenia,a meta-analysis was conducted,including five markers of oxidative stress [thiobarbituric reactive substances(TBArS),nitric oxide(NO),catalase(CAT),glutathione peroxidase(Gp) and superoxide dismutase(SOD)] in SCZ patients versus healthy *** study showed that TBArS and NO significantly increased in SCZ,while SOD activity significantly decreased in the disorganized type of SCZ *** significant effect size was found for the activities of Gp and CAT in SCZ patients(p>0.05).Egger’s regression test observed no significant publication bias across the oxidative stress markers,but found high heterogeneities in all the 5 *** subgroup analysis suggested that the ethnicity,sample size of patients and sample sources may contribute to the heterogeneity of the results for TBArS,NO and *** result further demonstrated the involvement of oxidative stress in the pathophysiology of schizophrenia.

关键词： schizophrenia oxidative stress meta-analysis lipid peroxidation nitric oxide antioxidant enzymes

proteomic dissection of biological pathways/processes through profiling protein-protein interaction networks

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Science china Chemistry 2010年第4期53卷 737-746页

作者： CHEN Xian institutes for biomedical sciences, fudan university, shanghai 200433, china Department of Biochemistry & Biophysics, School of Medicine, university of North Carolina-Chapel Hill, USA 1. institutes for biomedical sciences Fudan University Shanghai 200433 China2. Department of Biochemistry & Biophysics School of Medicine University of North Carolina-Chapel Hill Chapel Hill USA

Cellular functions, either under the normal or pathological conditions or under different stresses, are the results of the coordinated action of multiple proteins interacting in macromolecular complexes or assemblies. The precise determination of the specific composition of protein complexes, especially using scalable and high-throughput methods, represents a systematic approach toward revealing particular cellular biological functions. In this regard, the direct profiling protein-protein interactions (ppIs) represent an efficient way to dissect functional pathways for revealing novel protein functions. In this review, we illustrate the technological evolution for the large-scale and precise identification of ppIs toward higher physiologically relevant accuracy. These techniques aim at improving the efficiency of complex pull-down, the signal specificity and accuracy in distinguishing specific ppIs, and the accuracy of identifying physiological relevant ppIs. A newly developed streamline proteomic approach for mapping the binary relationship of ppIs in a protein complex is introduced.

关键词： Chen proteomic dissection of biological pathways/processes through profiling protein-protein interaction networks

Mass spectrometry analysis of melanoma related O-glycans in sera

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Science china Chemistry 2010年第4期53卷 807-811页

作者： BAI Chen1,4, WU Gang1 & ZHAO Liang2,3 1Department of Life Science, fudan university, shanghai 200433, china 2Department of Chemistry, fudan university, shanghai 200433, china 3institutes of biomedical sciences, fudan university, shanghai 200433, china 4Department of Food Science, shanghai Business School, shanghai 200235, china 1. Department of Life Science Fudan University Shanghai 200433 China4. Department of Food Science Shanghai Business School Shanghai 200235 China2. Department of Chemistry Fudan University Shanghai 200433 China3. Institutes of Biomedical Sciences Fudan University Shanghai 200433 China

Glycomics comparison was carried out by screening melanoma serum biomarkers between C57 mice with and without B16 implanted. O-glycans were released from 10 μL sera by β-elimination, purified by Graphitized Carbon Cartridge Solid phase Extraction (GCC-SpE) and analyzed by MALDI-QIT-TOF-MS. MS raw data were acquired and exported by Launchpad software. MATLAB was then applied for further data analysis. 10 Glycans were considered to have stable changes after B16 implantation and 5 of them were under structural analysis via MS/MS.

关键词： mass spectrometry glycomics melanoma biomarkers

DNA羟甲基化在小鼠胚胎干细胞中的研究进展

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生物化学与生物物理进展 2011年第7期38卷 611-614页

作者：谭理复旦大学生物医学研究院表观遗传学实验室上海200032

简要总结DNA羟甲基化在小鼠胚胎干细胞(mouse embryonic stem cells,mESC)中的最新研究进展.DNA甲基化(DNAmethylation)影响染色质的结构与功能,在发育与疾病发生过程中具有重要作用.2009年Tahiliani等发现TET1可以催化甲基化胞嘧啶(5-m... 详细信息

简要总结DNA羟甲基化在小鼠胚胎干细胞(mouse embryonic stem cells,mESC)中的最新研究进展.DNA甲基化(DNAmethylation)影响染色质的结构与功能,在发育与疾病发生过程中具有重要作用.2009年Tahiliani等发现TET1可以催化甲基化胞嘧啶(5-methylcytosine,5mC)氧化为羟甲基化胞嘧啶(5-hydroxymethylcytosine,5hmC).DNA羟甲基化(DNAhydroxymethylation)被认为是调节DNA甲基化的一种重要方式,成为了表观遗传学的研究热点之一.

关键词：小鼠胚胎干细胞 DNA羟甲基化 Tet1

proteomics in china:ready for prime time

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Science china(Life sciences) 2010年第1期53卷 22-33页

作者： GAO Xue1,ZHANG XueLi1,ZHENG JunJie1 & HE FuChu1,2 1 State Key Laboratory of proteomics,Beijing proteome research Center,Beijing Institute of radiation Medicine,Beijing 102206,china 2.institutes of biomedical sciences,fudan university,shanghai 200032,china 1. State Key Laboratory of proteomics Beijing Proteome Research Center Beijing Institute of Radiation Medicine Beijing 102206 China2. Institutes of Biomedical Sciences Fudan University Shanghai 200032 China

proteomics is a newborn science focusing on the comprehensive systematic analysis of all proteins in molecule machineries,organelles,cells,tissues,organs or intact *** has been becoming one of the focuses in life sciences and cutting-edge techniques in biotechnologies in the 21st *** the last decade,proteomics in china has developed much faster than other developing fields in the life *** review article briefly retrospects the origin and development of proteomics in china,and provides an overview of representative scientific progress and perspectives.

关键词： proteomics progress china Human proteome Organization Human Liver proteome project perspectives

Understanding the Mechanism of Adipogenesis

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Understanding the Mechanism of Adipogenesis

Control of anther cell differentiation and function by receptor-like protein kinases and transcriptional cascades

2008年全国生物化学与分子生物学学术大会

作者： Qi-qun Tang Xi Li Hai-yan Huang (Key Laboratory of Molecular Medicine,Ministry of Education,Department of Biochemistry and Molecular Biology,fudan university shanghai Medical School,shanghai 200032,p.r.china institutes of biomedical sciences,fudan university,shanghai 200032,p.r.china)

Obesity results from the increase of both adipocyte number and *** increase of adipocyte number is due to the recruitment of pluripotent stem cells that reside in the vascular stroma of adipose *** stem

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Control of anther cell differentiation and function by recep...