检索结果-南通市图书馆

A feature ensemble technology to identify molecular mechanisms for distinction between multiple subtypes of lymphoma

引用

Progress in Natural science:Materials International 2008年第12期18卷 1491-1500页

作者： Yueying Yang~(a,1) Haiyun Wang~(a,b,1) Xia Li~(a,b,c) Xue Xiao~c Su Fei~a Chuanxing Li~a Hongzhi Wang~d Shaoqi Rao~(a,c,e) Yadong Wang~d a College of Bioinformatics science and Technology,Bio-pharmaceutical Key Laboratory of Heilongjiang Province and State,Harbin medical university,Harbin 150081,china b College of life science and Technology,Tongji university,Shanghai 200092,china c The Biomedical Engineering institute,Capital Medieal university,beijing 100054,china d department of Computer science,Harbin institute of Technology,Harbin 150080,china e department of Molecular Cardiology,Cheveland Clinic Foundation,Cleveland,OH 44195,USA College of Bioinformatics science and Technology Bio-pharmaceutical Key Laboratory of Heilongjiang Province and State Harbin Medical University Harbin 150081 China College of life science and Technology Tongji University Shanghai 200092 China The Biomedical Engineering institute Capital Medical University Beijing 100054 China department of Computer science Harbin Institute of Technology Harbin 150080 China department of Molecular Cardiology Cleveland Clinic Foundation Cleveland OH 44195 USA

Due to complexities and genetic heterogeneities of biological phenotypes,robust computational approaches are desirable to achieve high generalization performance with multiple classifiers,perturbations of the data structures,and biological *** purpose of this study is to extend our developed ensemble decision approach to distinguish multiple heterogeneous phenotypes and to elucidate the underlying molecular bridges that intertwine the *** work identifies the significant molecular mechanisms (disease-relevant genes and functions) that underpin the complex molecular mechanisms for distinction between multiple phenotypes. Feature genes and hierarchical gene cores identified by our method have achieved high accuracy in the classification of multiple pheno- *** results show that the proposed analysis strategy is feasible and powerful in the classification of biological subtypes and in the explanation of the molecular connections between clinical *** interpretations with Gene Ontology revealed concerted genetic pathways for some lymphoma subtypes.

关键词： Ensemble decision approach Molecular mechanisms Lymphoma Microarray

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

耗竭性运动对大鼠骨骼肌线粒体内膜的影响

引用

生物化学与生物物理进展 1996年第3期23卷 281-283页

作者：颜宜茝张勇北京职工医学院生理学教研室北京体育大学体育生物科学系

观察ＳＤ大鼠一次急性运动至力竭后骨骼肌线粒体内膜流动性、ＮＡＤＨ－ＣｏＱ还原酶及ＡＴＰ酶活性变化．结果显示，大鼠骨骼肌线粒体内膜微粘度较安静时显著增高，线粒体内膜ＮＡＤＨ－ＣｏＱ还原酶和ＡＴＰ酶活性分别较安静时下降３... 详细信息

观察ＳＤ大鼠一次急性运动至力竭后骨骼肌线粒体内膜流动性、ＮＡＤＨ－ＣｏＱ还原酶及ＡＴＰ酶活性变化．结果显示，大鼠骨骼肌线粒体内膜微粘度较安静时显著增高，线粒体内膜ＮＡＤＨ－ＣｏＱ还原酶和ＡＴＰ酶活性分别较安静时下降３４．２％和４６．２％．研究提示，耗竭性运动后大鼠骨骼肌线粒体呼吸链内膜分子动力学和呼吸链酶组分活性变化，可能是运动性疲劳重要的膜分子特征．

关键词：运动生物化学耗竭性运动骨骼肌线粒体内膜

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Synthesis and Characterization of Polyurethane

引用

Journal of Wuhan university of Technology(Materials science) 2010年第6期25卷 984-986页

作者：贺纯静倪家骧 department of Pain Xuanwu HospitalCapital Medical University

Polyurethane was synthesized by the copolymerization with isocyanate and *** structure and properties of the copolymers were characterized by infrared spectrometer（IR）,differential scanning calorimetry（DSC） and thermogravimetry（TG）.The rabbit blood samples was dripped on the polyurethane,which was prepared by drying and die,and the contact angle was measured by contact angle measurement,then infiltration phenomenon was observed by electron *** results showed that the contact angle of blood samples and polyurethane was decreased with increasing time,and the platelets on the polyurethane would gradually increase and more *** the polyurethane coating had good hydrophilic property and anticoagulant activity.

关键词： polyurethane hydrophilicity anticoagulant

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

^(103)Pd-induced apoptosis of proliferative smooth muscle cells in bile ducts of dogs:significance and effects on related genes

引用

Hepatobiliary & Pancreatic Diseases International 2007年第5期6卷 521-526页

作者： Gui-Jin He,Fa-Qiang Yu,Rong Wu,Qin-Yi Gao,Shu-He Xu,Hong Gao, Wei-Guo Jiang,Tao Jiang and Xian-Wei Dai department of General Surgery,Second Affiliated Hospital(Shengjing Hospital),china medical university,Shenyang 110004,china,Radiotherapy Center,Second Affiliated Hospital(Shengjing Hospital),china medical university,Shenyang 110004,china,Pediatrics Surgery Laboratory,Second Affiliated Hospital(Shengjing Hospital),china medical university,Shenyang 110004,china,department of Pathology,Second Affiliated Hospital(Shengjing Hospital),china medical university,Shenyang 110004,china Isotope Unit,First Affiliated Hospital,china medical university,Shenyang 110001,china and Isotope Research institute,china institute of Atomic Energy,beijing 100050,china department of General Surgery Second Affiliated Hospital (Shengjing Hospital) China Medical University Shenyang 110004 China. 23318199@***

BACKGROUND:With the objective of developing a locally- produced radioactive stent,the present study used in vivo animal experiments to explore apoptosis of proliferative smooth muscle cells resulting from facilitation of the expression of genes caused byγ-radiation in order to prevent bile duct *** therefore explored the effects and significance ofγ-radiation on the activity of caspase-3,Fas and Bcl-2 genes in apoptosis of proliferative smooth muscle cells in the bile duct walls of dogs. METHODS:Twelve dogs were randomly divided into 2 groups(6 in each group).A postinjury bile duct stenosis model was established and radioactive 103 Pd( 103 palladium) or ordinary bile duct stents were implanted into the bile *** staining,RT-PCR and immunohistochemistry were used to detect the proliferation and apoptosis of bile duct smooth muscle cells in proliferative endomembrane and the expression of related caspase-3,Bcl-2 and Fas genes. RESULTS:The expression of caspase-3 and Fas genes in the bile duct tissues of dogs with radioactive stents was higher than that of dogs with ordinary *** was significant apoptosis of proliferative smooth muscle cells in the bile *** expression of the Bcl-2 gene in the bile duct tissues of dogs with radioactive stents was lower than that in those with ordinary *** was significant apoptosis of proliferative smooth muscle cells in the dogs with low Bcl-2 gene expression. CONCLUSIONS:Radiation increases the activity of caspase-3 and Fas genes and is associated with apoptosis. The radioactive 103 Pd stent may facilitate apoptosis of proliferative smooth muscle cells in the bile ducts of dogs by activating these *** Bcl-2 gene expression level is correlated with the occurrence of apoptosis and the radiosusceptibility of cells.

关键词： radioactive 103 Pd stent γ-radiation caspase-3 gene Fas gene Bcl-2 gene apoptosis

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

DNA barcoding provides distinction between Radix Astragali and its adulterants

引用

science china(life sciences) 2010年第8期53卷 992-999页

作者： GUO HaiYan1,2, WANG WeiWei2, YANG Ning2, GUO BaoLin3, ZHANG Sun2, YANG RuiJing2, YUAN Ye2, YU JunLin4, HU SongNian2, SUN QiShi1 & YU Jun2 1School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical university, Shenyang 110016, china 2Key Laboratory of Genome sciences and Information, beijing institute of Genomics, Chinese Academy of sciences, beijing 101300, china 3Chinese Academy of medical sciences, Peking Union medical College institute of Medicinal Plant Development, beijing 100094, china 4department of Chemistry, Tonghua Normal College, Tonghua 134002, china School of Traditional Chinese Materia Medica Shenyang Pharmaceutical University Shenyang China Key Laboratory of Genome sciences and Information Beijing Institute of Genomics Chinese Academy of Sciences Beijing China Peking Union medical College institute of Medicinal Plant Development Chinese Academy of Medical Sciences Beijing China department of Chemistry Tonghua Normal College Tonghua China

Based on variable nuclear and/or organellar DNA sequences among vastly divergent species as well as morphologically indistinguishable species, DNA barcoding is widely applicable in species identification, biodiversity studies, forensic analyses, and authentication of medicinal plants. The roots of Astragalus membranaceus and A. membranaceus var. mongholica are commonly used as Radix Astragali in several Asian countries, including china, Japan, and Korea. However, in addition to the two species recorded in the Chinese Pharmacopoeia, there are twenty-three species from different genera including Astragalus, Oxytropis, Hedysarum, and Glycyrrhiza, which have been used as adulterants not only in trading markets but also by the herbal medicine industry. Therefore, a simple, reliable, and accurate classification method is important for distinguishing authentic Radix Astragali from its adulterants. In this study, we acquired data for 37 samples from four related genera within the family Fabaceae. Then we compared four candidate DNA barcoding markers using ITS, matK, rbcL, and coxI sequences from nuclear, chloroplast, and mitochondrial genomes, all commonly used for plants to identify genetic variations among genera, intraspecies, and interspecies. We observed higher divergences among genera and interspecies for ITS, which have the average Kimura 2-parameter distances of 4.5% and 14.1%, respectively, whereas matK was found to have sufficient divergence at the intraspecific level. Moreover, two indels detected in the matK sequence are useful for PCR studies in distinguishing Radix Astragali from its adulterants. This study suggests that the combined barcoding regions of ITS and matK are superior barcodes for Radix Astragali and further studies should focus on evaluating the applicability and accuracy of such combined markers for a wide range of traditional Chinese herbs.

关键词： Radix Astragali DNA barcode Astragalus adulterant identification

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Effect of L-NAME on nitric oxide and gastrointestinal motility alterations in cirrhotic rats

引用

World Journal of Gastroenterology 2002年第2期8卷 328-332页

作者： Xin Wang Zong-You Zhang Mei Lan Ji-Yan Miao Xue-Gang Guo Yong-Quan Shi Yan-Qiu Zhao Jie Ding Kai-Cun Wu Dai-Ming Fan,institute of Digestive disease,Xijing Hospital,Fourth Military medical university,Xi’an 710032,Shaanxi Province,china Yue-Xia Zhong,Emergency department,Tangdu Hospital,Fourth Military medical university,Xi’an 710038,Shaanxi Province,china Ju Lu,Class EE 87,department of Electronic Engineering,tsinghua university,beijing 100084,china Bo-Rong Pan,Oncology Center,Xijing Hospital,Fourth Military medical university,Xi’an 710032,Shaanxi Province,china institute of Digestive Diseases Xijing Hospital Fourth Military Medical University Xi'an 710033 Shaanxi Province China.

AIM: To investigate the effect of L-NAME on nitric oxide and gastrointestinal motility alterations in cirrhotic rats. METHODS: Rats with cirrhosis induced by carbon tetrachloride were randomly divided into two groups, one n =13 receiving ***(-1) per day of N(G)-nitro-L-arginine methyl ester (L-NAME), a nitric oxide synthase inhibitor, for 10 days, whereas the other group (n =13) and control (n =10) rats were administrated the same volume of 9g.L(-1) saline. Half gastric emptying time and 2h residual rate were measured by SPECT, using (99m)Tc-DTPA-labeled barium sulfate as test meal. Gastrointestinal transition time was recorded simultaneously. Serum concentration of nitric oxide (NO) was determined by the kinetic cadmium reduction and colorimetric methods. Immunohistochemical SABC method was used to observe the expression and distribution of three types of nitric oxide synthase (NOS) isoforms in the rat gastrointestinal tract. Western blot was used to detect expression of gastrointestinal NOS isoforms. RESULTS: Half gastric emptying time and trans-gastrointestinal time were significantly prolonged(124.0 +/- 26.4 min; 33.7 +/- 8.9 min; 72.1 +/- 15.3 min; P2.4 +/- 0.5h; 9.5 +/- 0.3h; 8.2 +/- 0.8h; P2h residual rate was raised in cirrhotic rats than in controls and cirrhotic rats treated with L-NAME (54.9 +/- 7.6%,13.7 +/- 3.2%, 34.9 +/- 10.3%, P20 +/- 2.48) micromol.L(-1), (5.94 +/-1.07) micromol.L(-1) and control (5.66 +/- 1.60 micromol.L(-1), P<0.01. NOS staining intensities which were mainly located in the gastrointestinal tissues were markedly lower in cirrhotic rats than in the controls and cirrhotic rats after treated with L-NAME. CONCLUSION: Gastrointestinal motility was remarkably inhibited in cirrhotic rats, which could be alleviated by L-NAME. Nitric oxide may play an important role in the inhibition of gastrointestin

关键词： Animals Carbon Tetrachloride Digestive System Enzyme Inhibitors Gastrointestinal Motility Humans Liver Cirrhosis, Experimental Male NG-Nitroarginine Methyl Ester Nitric Oxide Nitric Oxide Synthase Random Allocation Rats Rats, Sprague-Dawley Research Support, Non-U.S. Gov't Tomography, Emission-Computed, Single-Photon

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

PATIENT-SPECIFIC BLOOD DYNAMIC SIMULATIONS IN ASSESSING ENDOVASCULAR OCCLUSION OF INTRACRANIAL ANEURYSMS

引用

Journal of Hydrodynamics 2009年第2期21卷 271-276页

作者： CHEN Jia-liang WANG Sheng-zhang DING Guang-hong YANG Xin-jian LI Hai-yun department of Mechanics and Engineering science Fudan University Shanghai 200433 China beijing institute of Neurosurgery Beijing 100050 China department of Biomedical Engineering Capital Medical University Beijing 100050 China

According to recent studies, there are various potential predictors for surgical outcome for cerebral aneurysms. An accurate surgical outcome assessment would help make better-informed decisions and avoid the risk of rebleeding. It is well known that hemodynamic factors play an important role in the pathogenesis and treatment of intracranial aneurysms. In this article, a computational fluid dynamic analysis is applied to one patient-specific model of the cerebral aneurysm located at the tip of basilar artery, by which the differences of hemodynamic parameters before and after endovascular treatment may be evaluated. Based on the model, we show that the flow behavior near the neck of the aneurysm sees great differences after endovascular treatment as compared with that before treatment, which also affects the wall shear stress and the displacement distribution. In addition, our whole simulation process is based on a series of CFD commercial software packages, which are easily available for doctors to implement such a method in their daily practice. These results would be used to assess the outcome of endovascular treatment for the aneurysm occlusion.

关键词： hemodynamics numerical simulation intracranial aneurysm

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Transcervical Approach for Resection of Lateral Skull Base Tumors

引用

Journal of Otology 2007年第2期2卷 102-108页

作者： LIU Jian-feng1, ZHANG Qiu-hang1, 2, YANG Da-zhang1, QU Qiu-yi2 1 department of Otorhinolaryngology, china Japan Friendship Hospital, beijing, china 2 department of Otorhinolaryngology Head & Neck Surgery, Xuanwu Hospital, Capital university of medical science, beijing, 100053, china department of Otorhinolaryngology China Japan Friendship Hospital Beijing China department of Otorhinolaryngology Head & Neck Surgery Xuanwu Hospital Capital University of Medical Science Beijing 100053 China

Objectives Conventional approaches for removal of lateral skull base tumors, including transmandibular, infratemporal fossa, preauricular transzygmatic subtemporal approaches, are major invasive procedures that often sacrifice hearing and cause abnormal occlusion and cosmetic defects. Reports of the transcervical approach for resection of skull base tumors are rare, although it was described for resection of clival chordomas in as early as 1966. The purpose of this study is to review our experiences in management of lateral skull base tumors using the transcervical approach. Study Design Retrospective chart review. Methods Six lateral skull base tumor cases treated with transcervical approach procedures were reviewed, including the medical records. Results There were 4 males and 2 females. Age ranged from 12 through 52 years. Histopathological diagnoses included malignant schwannoma(n = 1), malignant carotid body tumor(n = 1), heamangioma(n=1), schwannoma (n=2) and pleomorphic adenoma (n = 1). Transcervical techniques were used in all cases with the use of microscope in the lateral skull base area. Complete tumor removal was achieved in all cases. Postoperative radiotherapy was implemented in 1 case of malignant schwannoma and 1 case of malignant carotid body tumor. Jugular foramen syndrome occurred as a surgical complication in 1 case of malignant Schwannoma of the vagus nerve. There was no tumor recurrence during the 10 - 42 month follow-up period. Conclusion Compared with conventional approaches, the transcervical approach provides a easy, safe, minimal invasive and effective procedure for removal of selected lateral skull base tumors.

关键词： skull base tumor transcervical approach excision

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Effects of diffusion time and diffusion gradient strength on the mean diffusivity of water molecules in healthy human brain

引用

Progress in Natural science:Materials International 2006年第7期16卷 706-711页

作者： GAO Song, WANG Xiaoying and BAO Shanglian ( Laboratory of medical Physics & Engineering, Peking university, beijing 100871, china department of Radiology, The First Affiliated Hospital of Peking university, beijing 100034, china) Peking Univ Lab Med Phys & Engn Beijing 100871 Peoples R China Peking Univ Affiliated Hosp 1 Dept Radiol Beijing 100034 Peoples R China

Diffusion time and diffusion gradient strength are two important parameters of pulsed gradient spin echo (PGSE) that is the most commonly used method in diffusion-weighted magnetic resonance imaging. However, the effects of the diffusion time on the mean diffusivity (MD) in previous studies are controversial. In this study, a new experimental protocol was designed to evaluate the effects of the two parameters on the results of PGSE. It was found that MD decreased significantly with the increased diffusion time or increased diffusion gradient strength, which cannot be explained by the previous theories. Through theoretical analysis, we found the flaw in the pulsed gradient spin echo (PGSE) method, which is the explanation for the controversial results of diffusion time obtained by oth- ers.

关键词： diffusion time diffusion gradient strength diffusion-weighted imaging diffusion tensor imaging mean diffusivity.

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Human multi-drug resistant hepatocellular carcinoma induced in nude mice by B-ultrasonographically-directed orthotopic implantation:a new experimental model

引用

Hepatobiliary & Pancreatic Diseases International 2007年第4期6卷 393-398页

作者： Lei Ding,Xiao-Ping Chen,Zhi-Wei Zhang,Kai Jing and Wan-Guang Zhang Hepatic Surgery Center,Affiliated Tongji Hospital,Tongji medical College,Huazhong university of science and Technology,Wuhan 430030,china department of Oncology Center,beijing Shijitan Hospital,beijing 100038,china Hepatic Surgery Center Affiliated Tongji Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan 430030 China. dinglei1005@***

BACKGROUND:Present studies on the reversal of multi-drug resistance(MDR) are almost entirely limited to in vitro systems,and are seldom carried out in vivo. In order to study MDR under the in vivo situation,a MDR cell strain was used to establish a model in nude mice via orthotopic implantation directed by B-ultrasonography. This model is expected to provide a good platform for evaluating strategies to reverse the phenomenon of primary hepatocellular carcinoma(HCC) MDR. METHODS:An orthotopic MDR1 hepatoma was obtained by injecting the cell lines HepG2 and HepG2/ADM subserosally into the liver of nude mice(10 control and 20 MDR mice). The injections were made under B-ultrasonographic direction. Ultrasonography and laparotomy were used to assess tumor growth,and the long chain PCR technique was applied to sequence the MDR1 gene from multi-drug resistant human HCC cells,HepG2/ADM,and corresponding implanted tumor tissues. Furthermore,MDR1 mRNA and P-gp protein expression were evaluated by reverse transcriptase-polymerase chain reaction(RT-PCR),Western blotting,and immunohistochemical methods. RESULTS:The success rates of tumor implantation and induction of MDR were 100%(30/30) and 95%(19/20),respectively. The 3.8kbp MDR1 gene band was detected from the HepG2/ADM cell line and the correspondingimplanted tumor tissues,and the MDR1 gene sequence coincided with that reported in GenBank. The expressions of MDR1 mRNA and P-gp protein in MDR mice were significantly higher than those in the control group. There was a significant difference in P-gp protein expression between MDR and control mice. CONCLUSION:A MDR model has been successfully established in nude mice via orthotopic implantation of multi-drug resistant human HCC cells directed by ultrasonography.

关键词： nude mice orthotopic implantation carcinoma,hepatocellular drug resistance,multiple

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：