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Chinese Science Bulletin 2000年第19期45卷 1729-1734页

作者： SUN Dayeinstitute of molecular cell biology, hebei normal university, shijiazhuang 050016, china institute of molecular cell biology Hebei Normal University Shijiazhuang China

Generally, cell signal molecules are classified into the extracellular signal molecules (the first messengers) and the intracellular signal ones (the second messengers). Cyclic adenosine monophosphate (cAMP), calcium ions and calmodulin (CaM) are the traditional intracellular messengers, but they are also present in extracellular matrix (ECM). Some of them have been discovered to act as the first messengers through cell surface receptors. Other second messengers, such as cyclic guanosine monophosphate (cGMP), cyclic adenosine diphosphate ribose (cADPR) and annexin, are also found existing outside animal and plant cells. The existence of these messengers with intracellular-extracellular compatible functions in cells may be a regular biological phenomenon. These compatible messengers might be the communication factors between intracellular and extracellular regions or among the cell populations, and are also important in regulating cell development procedure.

关键词： intracellular signal extracellular signal compatible messenger.

Progress in the participation of Ca2+-calmodulin in heat shock signal transduction

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Progress in Natural Science:Materials International 2009年第10期19卷 1201-1208页

作者： Rengang Zhou a,b,Bing Li c,d,Hongtao Liu a,c,Daye Sun c,d,a institute of Genetics and Physiology,hebei Academy of Agricultural Sciences,shijiazhuang 050051,china.b hebei Provincial Key Laboratory of Plant Genetic Engineering,shijiazhuang 050051,china.c Academy of Life Sciences,hebei normal university,shijiazhuang 050016,china.d hebei Provincial Key Laboratory of molecular and cell biology,shijiazhuang 050016,china [a]institute of Genetics and Physiology Hebei Academy of Agricultural Sciences Shijiazhuang 050051 China [b]hebei Provincial Key Laboratory of Plant Genetic Engineering Shijiazhuang 050051 China [c]Academy of Life Sciences Hebei Normal University Shijiazhuang 050016 China [d]hebei Provincial Key Laboratory of molecular and cell biology Shijiazhuang 050016 China

A novel heat shock(HS) signal transduction pathway in plants for the participation of Ca2+-calmodulin(CaM) in HS signal trans-duction was *** induces a rapid increase in intracellular free calcium ion levels([Ca2+]i),and the involvement of phospholipase Cinositol 1,4,5-trisphosphate is one of the factors leading to elevation in [Ca2+]i induced by *** also increases the expression of the CaM gene and the accumulation of the CaM *** CaM isoform,AtCaM3,in Arabidopsis is a key member in the HS signal trans-duction ***3 regulates the activity of CaM-binding protein kinase(AtCBK3) or protein phosphatase(AtPP7),promoting the activation of the HS transcription factor,AtHSFA1a,by phosphorylation/dephosphorylation and the expression of heat shock pro-tein genes,then improving heat tolerance in plants.

关键词： Ca2+ Calmodulin Protein kinase Heat shock transcription factor Signal transduction

Primary evidence for involvement of IP3 in heat-shock signal transduction in Arabidopsis

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cell Research 2006年第4期16卷 394-400页

作者： Hong Tao Liu~(1,2,*) Fei Gao~(1,2,*) Shu Juan Cui~2 Jin Long Han~(1,2) Da Ye Sun~2 Ren Gang Zhou~1 ~1 institute of Genetics and Physiology,hebei Academy of Agricultural Sciences,shijiazhuang 050051,china.~2institute of molecular cell biology,hebei normal university,shijiazhuang 050016,china instituteofGeneticsandPhysiology HebeiAcademyofAgriculturalSciencesShijiazhuang050051China instituteofmolecularcellbiology HebeiNormalUniversityShijiazhuang050016China

The role of inositol 1,4,5-trisphosphate （IP3） in transducing heat-shock （HS） signals was examined in *** whole-plant IP3level increased within 1 min of HS at 37℃.After 3 min of HS,the IP3level reached a maximum2.5 fold *** the transgenic Arabidopsis plants that have AtHsp18.2plvmoter-β-glucuronidase （GUS） fusiongene,it was found that the level of GUS activity was up-regulated by the addition of caged IP3at both non-HS and HStemperatures and was down-regulated by the phospholipase C （PLC） inhibitors {1-[6-((17β-3-Methoxyestra-1,3,5（10）-trien-17-yl)amino)hexyl]-2,5-pyrrolidinedione} （U-73122）.The intracellular-free calcium ion concentration ([Ca2+]i) increased during HS at 37℃ in suspension-cultured Arabi-dopsis cells expressing *** with U-73122 prevented the increase of [Ca2+]ito some *** provided primary evidence for the possible involvement of IP3in HS signal transduction in higher plants.

关键词： heat shock signal transduction IP3 PLC [Ca2+]i Arabidopsis

Synthesis and Stereostructure of New β-Lactam Derivatives of1,5-Benzothiazepines

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Chinese Chemical Letters 1999年第6期10卷 447-448页

作者： Yuan LI Na SUN Sheng JIN(Department of Chemistry hebei university shijiazhuang 050016 institute of Chemistry and molecular Engineering Peking university Beijing 100871) hebei normal Univ Dept Chem Shijiazhuang 0550016 Peoples R China Peking Univ Inst Chem & Mol Engn Beijing 100871 Peoples R China

Reaction of 1,5-benzothiazepine with N-protected glycine gives new a-amino-β-lactamderivatives of 1.5-benzothiazepine. The configuration and conformation of the products wereconfirmed by x-ray diffraction. The resu... 详细信息

关键词： 1.5-Benzothiazepine β-lactam stereospecific reaction

拟南芥钙调素定点突变基因分离及其在钙不依赖钙调素结合蛋白检测中的应用(英文)

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生物化学与生物物理进展 2009年第7期36卷 890-896页

作者：高丽王振杰崔素娟河北师范大学分子细胞生物学研究室河北省分子细胞生物学重点实验室

动植物系统研究表明,钙调素不仅在结合钙离子时调节多种靶酶或靶蛋白的活性,而且没有钙离子结合时,还可以通过结合钙不依赖的钙调素结合蛋白,发挥多种生物学作用.然而,目前却没有体内分析钙调素与钙不依赖钙调素结合蛋白相互作用的方法... 详细信息

动植物系统研究表明,钙调素不仅在结合钙离子时调节多种靶酶或靶蛋白的活性,而且没有钙离子结合时,还可以通过结合钙不依赖的钙调素结合蛋白,发挥多种生物学作用.然而,目前却没有体内分析钙调素与钙不依赖钙调素结合蛋白相互作用的方法.首先,采用定点突变的方式,得到了拟南芥钙调素亚型2的多个突变基因mCaM2,随后,大肠杆菌重组表达突变蛋白的电泳迁移率及45Ca2+覆盖分析表明,得到了编码失去钙结合能力的钙调素的突变基因mCaM21234,mCaM21234突变钙调素中所有4个钙结合EF-hand结构域中的关键氨基酸谷氨酸均突变为谷氨酰胺.在酵母双杂交体系中,作为诱饵蛋白的突变钙调素mCaM21234与我们前期体外方法报道的钙不依赖性钙调素结合蛋白AtIQD26存在相互作用.这将为钙不依赖性钙调素结合蛋白提供有用的体内研究工具,有利于我们全面认识钙-钙调素-钙调素结合蛋白信号途径.

关键词：定点突变拟南芥钙调素钙不依赖钙调素结合蛋白

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植物类整合素蛋白研究进展

生物化学与生物物理进展 2001年第3期28卷 283-286页

作者：孙颖孙大业河北师范大学生命科学学院分子细胞生物学研究室石家庄050016

整合素是动物细胞膜上普遍存在的一类胞外基质受体 ,它所介导的粘附作用参与调节多种细胞功能 .近年来的研究发现在植物细胞中可能也存在类整合素 .综述了在植物类整合素检测、定位、组成、结构、基因以及生理功能研究方面所取得的初步... 详细信息

整合素是动物细胞膜上普遍存在的一类胞外基质受体 ,它所介导的粘附作用参与调节多种细胞功能 .近年来的研究发现在植物细胞中可能也存在类整合素 .综述了在植物类整合素检测、定位、组成、结构、基因以及生理功能研究方面所取得的初步进展与存在的问题 ,并与动物整合素的研究作了比较 .

关键词：类整合素植物质膜生理功能蛋白亚基

Immunoelectron microscopic localization of calmodulin in corn root cells

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cell Research 1993年第1期3卷 11-19页

作者： LI JIAXU, JIEWEN LIU, DAYE SUNDepartment of biology, hebei normal university,shijiazhuang 050016, china.3} of Hematology, Chinese Academy of MedicalSciences, Tianjin 300020, china institute of Hematology Chinese Academy of Medical Sciences Tianjin 300020 China

Methods for the localization of plant calmodulin by immuno-gold and immuno-peroxidase electron microscopy have been developed. In both corn root-cap cells and meristematic cells, calmodulin was found to be localized in the nucleus, cytoplasm, mitochondria as well as in the cell wall. In the meristematic cells, calmodulin was distinctly localized on the plasma membrane, cytoplasmic face of rough endoplasmic reticulum and polyribosomes. Characteristically, calmodulin was present in the amyloplasts of root-cap cells. The widespread distribution of calmodulin may reflect its pleiotropic functions in plant cellular activities.

关键词： calmodulin corn root cells immunoelectron-microscopic localization.

植物类受体激酶CrRLK1-L亚家族及其生物学功能

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生物化学与生物物理进展 2011年第10期38卷 891-899页

作者：韩永峰杨倩张胜伟孙大业孙颖河北师范大学分子细胞生物学研究所河北省分子细胞生物学重点实验室石家庄050016

植物CrRLK1-L亚家族类受体激酶的胞外域具有新颖结构基序,但功能大都未知.该家族成员广泛存在于被子植物中,但在动物和微生物中不存在其同源物.CrRLK1-L家族成员相对较少,但组织表达非常广泛.它们定位于细胞质膜上,并且部分成员的定位... 详细信息

植物CrRLK1-L亚家族类受体激酶的胞外域具有新颖结构基序,但功能大都未知.该家族成员广泛存在于被子植物中,但在动物和微生物中不存在其同源物.CrRLK1-L家族成员相对较少,但组织表达非常广泛.它们定位于细胞质膜上,并且部分成员的定位还具有极性,这与其参与雌雄配子体的识别和受精作用密切相关.该家族成员普遍具有激酶活性,该活性对其功能的发挥至关重要.目前仅报道在拟南芥中参与助细胞与花粉的识别和调控营养组织的细胞伸长,但参与这些生物学过程的作用机制似乎独立于已知的信号通路之外,可能有自身独特的信号传导机制.所以对这一类具特有结构基序的类受体激酶基因的功能研究,将有助于解析植物特有生物学过程的分子作用机制,特别是在植物有性生殖过程中,合理利用这些分子开展育种实践对未来农业生产具有潜在的应用价值.

关键词：类受体激酶 CrRLK1-L 信号传导

Identification and Characterization of a Rat Novel Gene RSEP4 Expressed Specifically in Central Nervous System

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Acta Biochimica et Biophysica Sinica 2004年第7期36卷 501-507页

作者： Xi-Dao WANG1,2, Ling-Wei KONG1, Zhi-Qin XIE1, Yu-Qiu ZHANG3, Zhi-Xin LIN2, Zhi-Qi ZHAO3,Lei YU4, and Nai-He JING1* 1 Laboratory of molecular cell biology, Laboratory of Stem cell biology, institute of Biochemistry and cell biology, Shanghai institutes for Biological Sciences, Chinese Academy of Sciences, Graduate School of the Chinese Academy of Sciences, Shanghai 200031, china.School of Life Science and Biotechnology, 2 Shanghai Jiaotong university, Shanghai 200030, china.institute of Neurobiology, Fudan university, Shanghai 200433, china.3 4 Department of cell biology, Neurobiology and Anatomy, College of Medicine, university of Cincinnati, Cincinnati, Ohio, USA Laboratory of molecular cell biology and Stem cell biology Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Graduate School of the Chinese Academy of Sciences Shanghai China

The low-abundantly expressed genes composed the majorities of the mRNAs expressed in the central nervous system (CNS), and were thought to be important for the normal brain functions. Through differential screening a low-abundance cDNA sublibrary with mRNA from neuropathic pain of chronic constriction injury (CCI) model, we have identified a novel rat gene, rat spinal-cord expression protein 4 gene (RSEP4). The total length of RSEP4 cDNA is 2006 bp, with a 501 nucleotide open reading frame (ORF) that encodes a 167 amino acid polypeptide. Northern blot revealed that RSEP4 was expressed specifically in the CNS. In situ hybridization showed that the mRNA of RSEP4 was strongly expressed in the CA1, CA2, CA3 and DG regions of hippocampus, the Purkinje cells of cerebellum, and the small sensory neurons of dorsal horn and large motor neurons of ventral horn of spinal cord. Over-expression of RSEP4-EGFP fusion protein in the human embryonic kidney 293T cells showed that RSEP4 protein was mainly localized in the cell cytoplasm. These results suggest that RSEP4 may play some roles in the CNS.

关键词： central nervous system (CNS) rat Northern blot in situ hybridization

molecular structure and biological function of proliferating cell nuclear antigen

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Progress in Natural Science:Materials International 2007年第10期17卷 1127-1135页

作者： Song Nanmeng1, Sang Jianli1 and Xu Heng2(1. institute of cell biology, College of Life Science, Beijing normal university, Beijing 100875, china.2. Emory university School of Medicine, GA 30332, USA) institute of cell biology College of Life Science Beijing Normal University Beijing 100875， China Emory university School of Medicine GA 30332 USA

Proliferating cell nuclear antigen (PCNA) is the core component of replication complex in eukaryote. As a processive factor of DNA polymerase delta, PCNA coordinates the replication process by interacting with various replication proteins. PCNA appears to play an essential role in many cell events, such as DNA damage repair, cell cycle regulation, and apoptosis, through the coordination or organization of different partners. PCNA is an essential factor in cell proliferation, and has clinical significance in tumor research. In this article we review the functional structure of PCNA, which acts as a function switch in different cell events.

关键词： PCNA Function Switch PCNA Binding Motif cell Cycle DNA Replication DNA Repair.