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Cell Research 2007年第4期17卷 283-285页

作者： Peng Jiang~1 Wenjing Du~1 Mian Wu ~1Hefei national laboratory for physical sciences at microscale and school of life sciences,university of science and technology of china.Anhui 230027,china Hefei national laboratory for physical sciences at microscale and school of life sciences University of Science and Technology of China Anhui 230027 China

The transcription factor p53 is a key regulator of theDNA damage response to genotoxic stress in higher eu-karyotes.Mutation/inactivation of p53 has been implicatedin the pathogenesis of many tumor types[1].p53 respondsto cellular insults,such as DNA damage or hypoxia,eitherby arresting the cell cycle so that DNA damage could berepaired,or by triggering apoptosis if DNA repair is futile.Transcriptional activation of apoptosis-related genes byp53 is critical for the induction of programmed cell death.In search of target genes of p53 involved in cell-cyclearrest and/or apoptosis,numerous p53-inducible geneshave been identified over the past 15 years.Among themare genes such as Bax[2],Puma[3],Noxa[4],Bid [5],p21waf1[6],and many others.These apoptosis-relatedfactors function in different cell organelles including thecytosolic membrane,the mitochondria and the cytosol.However,none of these candidate effectors alone can beaccountable for the complicated mechanisms underlyingthe p53 transcription-dependent apoptotic signaling path-ways.Earlier work implicated that Bad may contributeto p53-induced mitochondria-mediated apoptosis,yet noknown molecular mechanism had been ascribed to this

关键词： p53 and Bad gene remote strangers become close friends

Syntheses of amphiphilic biodegradable copolymers of poly(ethyl ethylene phosphate) and poly(3-hydroxybutyrate) for drug delivery

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science china.Chemistry 2009年第7期52卷 961-968页

作者： CHENG Jing1 & WANG Jun2 1 Department of Polymer science and Engineering, university of science and technology of china. Hefei 230026, china.2 Hefei national laboratory for physical sciences at microscale, school of life sciences, university of science and technology of china. Hefei 230027, china Department of Polymer science and Engineering University of Science and Technology of China Hefei China Hefei national laboratory for physical sciences at microscale School of Life Sciences University of Science and Technology of China Hefei China

Biodegradable and amphiphilic triblock copolymers poly(ethyl ethylene phosphate)-poly(3-hydroxybutyrate)-poly(ethyl ethylene phosphate) (PEEP-b-PHB-b-PEEP) have been successfully synthesized through ring-opening polymerization. The structures are confirmed by gel permeation chromatography and NMR analyses. Crystallization investigated by X-ray diffraction reveals that the block copolymer with higher content of poly(ethyl ethylene phosphate) (PEEP) is more amorphous, showing decreased crystallizability. The obtained copolymers self-assemble into biodegradable nanoparticles with a coreshell micellar structure in aqueous solution, verified by the probe-based fluorescence measurements and transmission electronic microscopy (TEM) observation. The hydrophobic poly(3-hydroxybutyrate) (PHB) block serves as the core of the micelles and the micelles are stabilized by the hydrophilic PEEP block. The size and size distribution are related to the compositions of the copolymers. Paclitaxel (PTX) has been encapsulated into the micelles as a model drug and a sustained drug release from the micelles is observed. MTT assay also demonstrates that the block copolymers are biocompatible, rendering these copolymers attractive for drug delivery.

关键词： poly(3-hydroxybutyrate) polyphosphoester drug delivery polymeric nanoparticles

Chronic morphine exposure induces degradation of receptive field properties of LGN cells in cats

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Acta Pharmacologica Sinica 2005年第9期26卷 1034-1038页

作者： Li-hua HE~2 Guang-xing LI~2 Xiang-mi LI~2 Yi-feng ZHOU~(2,3,4)2 Hefei national laboratory for physical sciences at microscale and school of life science,university of science and technology of china.Hefei 230027,china.3 laboratory of Visual Information Processing,Institute of Biophysics,Chinese Academy of sciences,Beijing 100101,china Hefei national laboratory for physical sciences at microscale and school of life science University of Science and Technology of China Hefei China laboratory of Visual Information Processing Institute of Biophysics Chinese Academy of Sciences Beijing China

Aim:To investigate the effect of chronic morphine exposure on the receptive fieldproperties of lateral geniculate nucleus(LGN)neurons in cats.Methods:Catswere injected with morphine(10 mg/kg)or saline twice daily,for 10 d.Subsequently,extracellular single-unit recording techniques were used to examine the sensitivityof LGN neurons to visual stimuli in chronic morphine-treated and saline-treatedcats.Results:Compared with saline-treated cats(as controls),LGN neurons inmorphine-treated cats had decreased signal-to-noise ratios(1.9 vs 3.1 ),and orien-tation and direction sensitivity(0.103 vs O.135 and 0.074 vs O.10,respectively),accompanied by significant increases in spontaneous(27.4 vs 17.5)and evokedactivity(preferred:42.2 vs 38.1;average:28.1 vs 22.3).Conclusion:Chronic mor-phine exposure can lead to the functional degradation of LGN neurons in cats,which might result from the effects of chronic morphine exposure on inhibitoryneurotransmission.

关键词： direction sensitivity orientation sensitivity signal-to-noise ratio spontaneous response evoked response

Short exposure to paclitaxel induces multipolar spindle formation and aneuploidy through promotion of acentrosomal pole assembly

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science china.life sciences) 2010年第11期53卷 1322-1329页

作者： BIAN MingLei1, FU JingYan1, YAN Yan1, CHEN Qiang1, YANG Chao2, SHI QingHua2,JIANG Qing1 & ZHANG ChuanMao1 1MOE Key laboratory of Cell Proliferation and Differentiation and the State Key laboratory of Bio-membrane and Membrane Bio-engineering, College of life sciences, Peking university, Beijing 100871, china.2Hefei national laboratory for physical sciences at microscale and school of life sciences, university of science and technology of china. Hefei 230026, china MOE Key laboratory of Cell Proliferation and Differentiation and the State Key laboratory of Bio-membrane and Membrane Bio-engineering College of Life Sciences Peking University Beijing China Hefei national laboratory for physical sciences at microscale and school of life sciences University of Science and Technology of China Hefei China

Paclitaxel is a widely used microtubule drug and cancer medicine. Here we report that by short exposure to paclitaxel at a low dose, multipolar spindles were induced in mitotic cells without centrosome amplification. Both TPX2 depletion and Aurora-A overexpression antagonized the multipolarity. Live cell imaging showed that some paclitaxel-treated cells accomplished multipolar cell division and a portion of the daughter cells went on to the next round of mitosis. The surviving cells grew into clones with varied genome content. The results indicated that an aneuploidy population could be induced by short exposure to paclitaxel at a low dose, implicating potential side effects of paclitaxel.

关键词： paclitaxel tpx2 Aurora-A multipolar cell division aneuploidy

Bcl-2 cleavages at two adjacent sites by different caspases promote cisplatin-induced apoptosis

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Cell Research 2007年第5期17卷 441-448页

作者： Jianbei Zhu1 Ying Yang1 Jiarui Wu1,21 Kev laboratory of Systems Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological sciences, Chinese Academy of sciences, 320 Yueyang Road, Shanghai 200031, china. Hefei national laboratory for physical sciences at microscale and school of life sciences, university of science & technology of china, Hefei 230027, china Key laboratory of Systems Biology Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai China Hefei national laboratory for physical sciences at microscale and school of life sciences University of Science & Technology of China Hefei China

The protein encoded by bcl-2 proto-oncogene plays an important role in the mitochondria-mediated apoptotic pathway.Although the general role of Bcl-2 is anti-apoptotic, previous work showed that Bcl-2 fragments cleaved by caspasescould promote apoptotic process. We report herein that Bcl-2 protein was cleaved to produce two fragments of around23 kDa in human hepatocarcinoma BEL-7404 cells or in Bcl-2 overexpressing CHO cells induced by cisplatin. Treat-ing cells with the general caspase inhibitor z-VAD-fmk blocked the induced cleavage of Bcl-2. Mutagenesis analysesshowed that Bcl-2 was cleaved by caspases at two adjacent recognition sites in the loop domain (YEWD↓AGD↓V),which could be inhibited by caspase-8 and -3 inhibitors, respectively. Overexpression of the carboxyl terminal 23 kDafragments increased the sensitivity of CHO cells to cisplatin-induced apoptosis. These results indicate that Bcl-2 can becleaved into two close fragments by different caspases during cisplatin-induced apoptosis, both of which contribute tothe acceleration of apoptotic process.

关键词： Bcl-2 apoptosis cisplatin caspase-3 caspase-8

High Expression of NKG2A/CD94 and Low Expression of Granzyme B Are Associated with Reduced Cord Blood NK Cell Activity

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Cellular & Molecular Immunology 2007年第5期4卷 377-382页

作者： Yanyan Wang1,2,Han Xu1,2,Xiaodong Zheng1,Haiming Wei1,Rui Sun1 and Zhigang Tian1,31Institute of Immunology,Hefei national laboratory for physical Materials at microscale and school of life sciences,university of science and technology of china.Hefei 230027,china.2Yanyan Wang and Han Xu equally contributed to the present study Institute of Immunology Hefei National Laboratory for Physical Materials at Microscale School of Life Sciences University of Science and Technology of China Hefei 230027 China.

Human umbilical cord blood （CB） has recently been used as a source of stem cells in transplantation. NK cells derived from CB are the key effector cells involved in graft-versus-host disease （GVHD） and graft-versus-leukemia （GVL）. It was reported that the activity of CB NK cells was lower than that of adult peripheral blood （PB） NK cells. In this study, we analyzed the expression of some NK cell receptors and cytotoxicity-related molecules in CB and PB NK cells. The expressions of activating NK receptors, CD16, NKG2D and NKp46, did not show significant difference between CB and PB NK cells. But the expression of inhibitory receptor NKG2A/CD94 was significantly higher on CB NK cells. As to the effector function molecules, granzyme B was expressed significantly lower in CB NK cells, but the expressions of intracellular perforin, IFN-γ, TNF-α and cell surface FasL and TRAIL did not show difference between CB and PB NK cells. The results indicated that the high expression of NKG2A/CD94 and low expression of granzyme B may be related with the reduced activity of CB NK cells.

关键词： NK cord blood NK cell receptor NKG2A granzyme B cytotoxicity

Solution structure and function of proteins relevant to pre-mRNA splicing

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生物物理学报 2009年第S1期25卷 76-76页

作者： Yunyu Shi, Jihui Wu, Xiaojuan Huang, Xingsheng Wang Hefei national laboratory for physical sciences at microscale and school of life sciences, university of science and technology of china. Hefei, Anhui, 230026, P.R.china

Pre-mRNA splicing is a dynamic process. It is catalyzed by the spliceosome which is a large machine formed by an ordered interactions of several small nuclear ribonucleoproteins, U1,

关键词： mRNA Solution structure and function of proteins relevant to pre-mRNA splicing

Small Molecule Autophagy Inducers:A New Therapeutic Paradigm for Neural Degeneration Diseases?

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Small Molecule Autophagy Inducers:A New Therapeutic Paradigm...

Protein-protein interaction studied by NMR

第五届全国化学生物学学术会议

作者： Long-Ping Wen Hefei national laboratory for physical sciences at microscale and school of life sciences,university of science and technology of china.Hefei,Anhui 230027,china

＜正＞In contrast to the ubiquitin-proteasome system which is the major cellular route for the degradation of short-lived proteins,autophagy is the major cellular route for the degradation of long-lived proteins and cytoplasmic organelles.This dynamic process involves the rearrangement of subcellular membranes to sequester cytoplasm and organelles for delivery to the lysosome where

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Protein-protein interaction studied by NMR