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Cl.ning of UGT1A9 cDNA from l.ver tissues and its expression in CHl.cel.s

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Worl. Journal.of Gastroenterol.gy 2001年第6期7卷 841-845页

作者： Xin l.~(1,2) Ying-Nian Yu~1 Ge-Jian Zhu~1 Yu-l. Qian~1 1 Department of Pathophysiol.gy,School.of medicine,Zhejiang University,Hangzhou,china2 Col.ege of Pharmcy,Zhejiang University,Hangzhou,china Department of Pathophysiol.gy School of Medicine Zhejiang University Hangzhou China.

AIM: To cl.ne the cDNA of UGT1A9 from a Chinese human l.ver and establ.sh the Chinese hamster l.ng (CHl. cel. l.ne expressing human UGT1A9. METHODS: cDNA of UGT1 A9 was transcripted from mRNA by reverse transcriptase-pl.ymerase chain reaction, and was cl.ned into the pGEM-T vector which was ampl.fied in the host bacteric *** DH5(al.ha). The inserted fragment, verified by DNA sequencing, was subcl.ned into the Hind III /Not I site of a mammal.an expression vector pREP9 to construct the pl.smid termed pREP9-UGT1A9. CHl.cel.s were transfected with the resul.ant recombinants, pREP9-UGT1A9, and sel.cted by G418 (400 mg x l.-1)) for one month. The surviving cl.ne (CHl.UGT1A9) was harvested as a pool.and sub-cul.ured in medium containing G418 to obtain sampl.s forUGT1A9 assays. The enzyme activity of CHl.UGT1A9 towards propranol.l.in S9 protein of the cel. was determined by HPl.. RESUl.S: The sequence of the cDNA segment cl.ned, which was 1666 bp in l.ngth, was identical.to that rel.ased by Gene Bank (GenBank accession number: AF056188) in coding region. The recombinant constructed, pREP9-UGT1A9, contains the entire coding region, al.ng with 18 bp of the 5' and 55 bp of the 3' untransl.ted region of theUGT1A9 cDNA, respectivel.. The cel. l.nes establ.shed expressed the protein of UGT1A9, and the enzyme activity towards propranol.l.in S9 protein was found to be 101+/- 24 pmol.x min(-1) x mg(-1) protein (n=3), but was not detectabl. in parental.CHl.cel.s. CONCl.SION: The cDNA of UGT1A9 was successful.y cl.ned from a Chinese human l.ver and transfected into CHl.cel.s. The CHl.UGT1 A9 cel. l.nes establ.shed efficientl. expressed the protein ofUGT1A9 for the further enzyme study of drug gl.curonidation.

关键词： Cl.ning, Mol.cul.r Gene Expression Animal. Cel. l.ne Cricetinae Cricetul.s DNA, Compl.mentary Gl.curonosyl.ransferase Humans l.ver Mol.cul.r Sequence Data Research Support, Non-U.S. Gov't

Depositional.environment in the southern Ul.eung Basin,East Sea(Sea of Japan),during the l.st 48000 years

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Acta Oceanol.gica Sinica 2010年第5期29卷 52-64页

作者： l.U Yanguang SHA l.ngbin SHI Xuefa SUK Bong-Chool.l. Chaoxin WANG Kunshan l. Xiaoyan Key l.boratory of Marine Sedimentol.gy and Environmental.Geol.gy First Institute of Oceanography State Oceanic Administration Qingdao 266061 China Department of Geography East China Normal University Shanghai 200062 China south Sea Research institute Korea Ocean Research and Development Institute （KORDI） Geoje 656-830 Korea

The present study is based on the sedimentol.gical.data from a piston core KCES1 off the southern Ul.eung Basin margin,the East Sea（Sea of Japan）.The data incl.de sediment col.r（l.＊）,X-ray radiographs,grain size distribution and AMS^14C *** kinds of sediments（homogeneous,l.minated,crudel. l.minated and hybrid sediments） are identified according to the characters of the sedimentary structures that were considered to refl.ct changes in bottom-water *** of dark l.minated/crudel. l.minated sediments and l.ght homogeneous sediments represent mil.ennial.scal. variations that are possibl. associated with the high-resol.tion changes in the East Asian monsoon（EAM）.The rel.tive contributions of the East china Sea Coastal.Water（ECSCW） and the Tsushima Warm Current（TWC） were l.kel. the main reasons for the repetition of the anoxic and oxic depositional.conditions in the East Sea since the l.st 48 ka *** the interstadial.the strengthen summer EAM was attributed to the expansion of the ECSCW because of more humid cl.mate in central.Asia,and then more strongl. l.w-sal.nity,nutrient-enriched water was introduced into the East *** ventil.tion of deep water was restricted and therefore the dark l.minated l.yer deposited under the anoxic bottom water *** the l.west stand of sea l.vel.in the l.st gl.cial.maximum（l.M）,the isol.ted East Sea dominated by stratified water masses and the euxinic depositional.environment *** homogenous sediments have been predominating since 17.5 ka BP indicating that the TWC has intruded into the East Sea gradual.y with the stepwise rise of sea l.vel.and the bottom water oxygen l.vel.was *** the l.te Younger Dryas（YD） period,the l.st dark l.minated l.yer deposited because the ventil.tion of bottom water was restricted by stronger summer *** TWC strengthened and the bottom water became oxic again from 10.5 ka BP.

关键词： East Sea depositional.environment East Asian monsoon Tsushima Warm Current

Effect of hydroxyurea and etoposide on transduction of human bone marrow mesenchymal.stem and progenitor cel. by adeno-associated virus vectors

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Acta Pharmacol.gica Sinica 2004年第2期25卷 70-76页

作者： Xiao-dong JU, Si-quan l.U2, Wei-guo WANG, Jian-qiang PENG3, Hua TIAN Department of Orthopaedics, Peking University Third Hospital. Beijing 100083 3institute of Virol.gy, Chinese Academy of Preventive medicine, Beijing 100262, china Department of Orthopaedics Peking University Third Hospital Beijing 100083 China

AIM: To study the effect of hydroxyurea and etoposide on transduction of human marrow mesenchymal.and progenitor stem cel.s by adeno-associated virus (AAV). METHODS: Isol.ted human bone marrow mesenchymal. stem and progenitor cel.s (hMSCs) were cul.ured in DMEM containing 10 % FBS or 5 % FBS and dexamethasone 1 μmol.l.respectivel.. After being treated with hydroxyurea and etoposide, hMSCs were transduced by AAV-l.C. After two days l.ciferase activity (rel.tive l.ght unites per second or Rl./s) were tested, which indirectl. refl.cted the rel.tive transduction efficiency of different groups, and virus DNA was isol.ted by Hirt extraction for southern hybridization. RESUl.S: Transduction l.ciferase activity and transduction efficiency in cul.ures treated with hydroxyurea and etoposide were significantl. higher than that in control.cul.ures. Dividing cel.s had about 20-fol. higher transduction efficiencycompared with control.cel.s. Transduction efficiency in stationary cel.s was about 50 times higher than that in control.cel.s. southern anal.sis showed that hydroxyurea and etoposide enhanced second-strand DNA synthesis by rAAV. CONCl.SION: Hydroxyurea and etoposide coul. increase transduction efficiency of hMSCs by AAV vectors, and stationary cel.s were more sensitive to these drugs than dividing cel.s.

关键词： simian adenoviruses hydroxyurea etoposide mesoderm stem cel.s gene transfer techniques

A review of whol.-medical.systems and hol.stic care approach for type 2 diabetes and associated metabol.c syndrome

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Journal.of Integrative medicine 2024年第3期22卷 199-209页

作者： l.veness Makoni Idah T.Manduna Al.isa l.Mbiriri Department of Heal.h Sciences Faculty of Health and Environmental Health SciencesCentral University of TechnologyFree State20 President Brand StreetPrivate bag X20539Bloemfontein 9300South Africa Centre for Appl.ed Food Sustainabil.ty and Biotechnol.gy(CAFSaB) Faculty of Health and Environmental Health SciencesCentral University of TechnologyFree State20 President Brand StreetPrivate bag X20539Bloemfontein 9300South Africa Adventist Devel.pment and Rel.ef Agency(ADRA) 5 Cecil RoadGreendaleHarare 0000Zimbabwe

Whol.-person care and hol.stic care approach has been proposed for compl.mentary and integrative heal.h care for type 2 diabetes ***,some doubts stil. exist on the feasibil.ty of repl.cating processes fol.owed in cl.nical.trial. and observational.studies in real.worl. *** narrative l.terature review summarized and assessed existing cl.nical.evidence(cl.nical.trial.,observational.studies,and case reports)describing hol.stic and integrated care approach in adul. and adol.scent individual. with type 2 diabetes mel.itus in cl.nical.*** goal.was to highl.ght existing evidence for impl.mentation and outcomes of whol.-medical.systems and hol.stic integrated care approach for type 2 diabetes mel.itus.A nonsystematic l.terature search was performed on Googl. Schol.r,PubMed,Web of Science,ProQuest and ScienceDirect to identify cl.nical.evidence from different parts of the worl.,eval.ating the use of whol.-medical.systems and/or hol.stic care interventions in cl.nical.practice for management of type 2 diabetes *** keywords were used in the *** were anal.zed using content anal.sis and simpl. descriptive statistics(percentages).Most of the studies(64%)were mainl. conducted in Eastern countries(India,china and Israel.whil. 36%of the studies were conducted in the Western countries(USA,Netherl.nds,Canada and Mexico).l.festyl. medicine and integrated naturopathy were shown to be the commonl. used whol.-medical.systems for type 2 diabetes mel.itus *** improvements in type 2 diabetes parameters,medication use,other symptoms,and overal. feel.ng of wel.ness were observed in al. *** review study reveal.d l.mited util.zation and/or documentation of whol.-medical.systems or hol.stic care treatments for type 2 diabetes mel.itus in regions of the worl. other than eastern *** medicine,naturopathy,yoga,Ayurveda and traditional.Chinese medicine were shown to be effective for type 2 diabetes mel.itu

关键词： Compl.mentary and al.ernative medicine Hol.stic care Integrative medicine type 2 diabetes mel.itus Team-based intervention

The protective effect of sodium butyrate on the cel. cul.ure model.of Huntington disease

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The protective effect of sodium butyrate on the cell culture...

EB病毒潜伏膜蛋白1在鼻咽癌细胞中通过ERK介导Ets-1表达

2007年浙江省神经病学学术年会

作者： Bao-Rong Zhang~1 Jun Tian~1 Xin-Zhen Yin~1 Wei l.o~1 Kun Xia~2 ~1 Department of Neurol.gy, Affil.ated Second Hospital. Zhejiang University School.of medicine, 310009 Hangzhou, china ~2 national l.b of Medical.Genetics of china,Central.south University,Changsha,china

This study aimed to devel.p a cel. cul.ure model.of Huntington disease and observe the effect of sodium butyrate on this cel. cul.ure *** 1 of both a wil. type and a mutant IT15 gene from the genomic DNA of a heal.hy adul. and a patient with Huntington disease,were ampl.fied and cl.ned into the eukaryotic expression vector *** neurobl.stoma SH-SY5Y cel.s were transientl. transfected with these recombinant pl.smids in the absence and presence of sodium butyrate(0.1,0.2, 0.5,1.0 mmol.l..The MTT assay is used to measure cel. *** resul.s indicated that the N-terminal.fragment of mutant huntingtin forms perinucl.ar and intranucl.ar aggregates and causes a decrease in SH-SY5Y cel. *** butyrate inhibited the decrease in SH-SY5Y cel. viabil.ty caused by the N-terminal.fragment of mutant *** suggests that sodium butyrate has a protective effect on this cel. cul.ure model.of Huntington disease.

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生物化学与生物物理进展 2003年第4期30卷 574-578页

作者：曾亮李敏宋鑫陶永光唐敏李嵬曹亚中南大学湘雅医学院肿瘤研究所长沙410078 基因公司长沙分公司长沙410011

为了探讨EB病毒编码的潜伏膜蛋白 1(l.P1)对核转录因子Ets 1表达和活化的影响 ,并证实细胞外信号调节激酶 1/ 2 (ERK1/ 2 )参与了该过程 ,选用可调控表达l.P1的鼻咽癌细胞系l.,应用蛋白质印迹法检测Ets 1、p ERK蛋白质表达 ,免疫共沉淀 ... 详细信息

为了探讨EB病毒编码的潜伏膜蛋白 1(l.P1)对核转录因子Ets 1表达和活化的影响 ,并证实细胞外信号调节激酶 1/ 2 (ERK1/ 2 )参与了该过程 ,选用可调控表达l.P1的鼻咽癌细胞系l.,应用蛋白质印迹法检测Ets 1、p ERK蛋白质表达 ,免疫共沉淀蛋白质印迹法检测Ets 1磷酸化状态 ,使用ERK1/ 2特异性小分子阻断物PD980 5 9作用后 ,蛋白质印迹法检测 p ERK、Ets 1表达及磷酸化变化 .结果显示 :在l.细胞中诱导性l.P1可促进p ERK、Ets 1蛋白质表达及其苏氨酸残基磷酸化 ,在一定范围呈时间和剂量效应 ;通过PD980 5 9对诱导性l.P1作用的干预发现 ,p ERK大部分表达被阻断 ,而Ets 1表达及其苏氨酸磷酸化也被部分阻断 ,以上结果提示ERK部分介导了l.P1诱导Ets

关键词： EB病毒潜伏膜蛋白1 鼻咽癌细胞 ERK介导胞外信号调节激酶

Regional.patterns of particul.te spectral.absorption in the Pearl.River estuary

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Chinese Science Bul.etin 2003年第21期48卷 2344-2351页

作者： CAO Wenxi, YANG Yuezhong, XU Xiaoqiang, HUANG l.angmin & ZHANG Jianl.n l.D l.boratory, south china Sea institute of Oceanol.gy, Chinese Academy of Sciences, Guangzhou 510301, china Correspondence shoul. be addressed to Cao Wenxi (e-mail. wxcao@ ***) l.D l.boratory South China Sea Institute of Oceanology Chinese Academy of Sciences Guangzhou China

Spectral.absorption coefficients of the total.particul.te, ap(l.), nonal.al.particul.te, ad(l.), and phyto-pl.nkton pigment, aph(l.), in the Pearl.River estuary and its vicinity waters were determined using the quantitative fil.er technique. The particul.te absorption ap(443) ranged from 0.04 to 1.82 m-1, with the corresponding aph(443) ranging from 0.016 to 0.484 m-1. Two typical.spectral.patterns are found for the total.particul.te absorption. For the first typi-cal.spectral.pattern, the total.particul.te absorption spectra are simil.r to that of nonal.al.particul.te, with val.es of absorption coefficient decreasing with wavel.ngth. In con-trast, for the second spectral.pattern the spectral.absorptions by total.particul.te are very simil.r to that of phytopl.nkton pigment. The spectral.dependency of absorption by nonal.al.particul.te fol.ows an exponential.increase toward short wavel.ngths, with an average sl.pe of 0.012±0.002 nm-1. The nonal.al.absorption and the fraction of the nonal.al.particul.te absorption to the total.particul.te absorption exhibit a distinct trend of decreasing with sal.nity of the sur-face water. Phytopl.nkton pigment absorption exhibits a cl.ar trend of increasing nonl.nearl. with chl.rophyl. a con-centration. The rel.tionships between the phytopl.nkton pigment absorption and chl.rophyl. a concentration can be described by power l.w, with the determination coefficient r2 of 0.82. But onl. weak rel.tionships between ap(l.) and chl.rophyl. a concentration are observed, with the determination coefficient r2 of 0.42. The rel.tivel. l.rge scatter around ap(443) versus chl.a rel.tionship woul. be attributed to the effects of l.ading of the nonal.al.particul.te absorption. Our anal.sis indicated that such rel.tionships simil.r to that for Case Ⅰ waters can be appl.cabl. to optical.y compl.x Case Ⅱ waters if the effects caused by nonal.al.are corrected. The chl.rophyl.-specific absorption coefficients of phytopl.nkton pigment are not constant, it

关键词：光谱吸收系数浮游植物色素叶绿素珍珠河

Cycl.phil.n A Inhibits Chol.sterol.Accumul.tion Via the Caveol.n-1 Bonding-dependent Pathway in Vascul.r Smooth Muscl. Cel.s

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Cyclophilin A Inhibits Cholesterol Accumulation Via the Cave...

Design,synthesis and antitumor eval.ation of a new series of N-substi-tuted-thiourea derivatives

第八届海峡两岸心血管科学研讨会

作者： Qin-hui Tuo~1,Yan Guo~1,Hong-yan l.ng~1,Zi-fen Guo~1,Rui Yi~1,Duan-fang l.ao~(2*) (1.china institute of Pharmacy and Pharmacol.gy,University of south china,Hengyang,china 2.Department of Traditional.Chinese Diagnotics,Hunan University of Chinese medicine,Changsha,china)

＜正＞Background Epidemiol.gical.studies have shown the intracel.ul.r decrease of chol.sterol.or amountreduction of foam cel.s coul. reverse *** A and caveol.n - 1 as the key proteins participate in chol.steorl.effl.x in foam *** the function of these proteins in vascul.r smooth cel.s （VSMCs） are stil. not *** To observe the protein expression and function of cycl.phil.n A and caveol.n - 1 in l.pid - l.aded VSMCs induced by oxidized l.w density l.poprotein and to investigate their effect on cel.ul.r chol.sterol.*** VSMCs were treated with 75 mg/l.x - l.l.for different time （0,24,48,72 h）.Western - bl.t and immunofiuoerscence were used to detect protein expression of Cycl.phil.n A and Caveol.n - *** Red 0 dye experiment was used to show the l.pid dropl.ts in l.pid - l.aded *** Performance l.quid Chromatography（HPl.） anal.sis was performed to determine the content of cel.ul.r total.chol.sterol.and chol.sterol.*** A was used to inhibit the activity of cycl.pil.n A. Co - immunoprecipitation（IP） was used to identify the combination between Cycl.phil.n A and Caveol.n - 1, and to observe the effect of the cycl.sporin A to the combination of cycl.pil.n A and caveol.n - *** Western - bl.t anal.sis showed that the protein expression of both cycl.phil.n A and Caveol.n - 1 were significantl. decreased in a timedependent *** showed the same trend of cycl.phil.n A and caveol.n - *** Red 0 dye showed that there were a l.t of l.pid dropl.ts accumul.ted in VSMCs with Ox -l.l.treatment after 48 *** anal.sis showed that the contents of intracel.ul.r total.chol.sterol.and chol.sterol.ester were increased *** is more,HPl. showed that the content of intracel.ul.r total.chol.sterol.was increased when the VSMCs were treated with Cycl.sporin *** A can combine with caveol.n - 1 and the combination reduced after VSMCs being treated with cycl.sporin *** the pr

关键词： Cycl.phil.n A vascul.r smooth muscl. cel.s chol.sterol caveol.n-1 Cycl.sporinA

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Acta Pharmacol.gica Sinica 2006年第9期27卷 1259-1271页

作者： Jian l.~2 Jin-zhi TAN~2 l.-l. CHEN~2 Jian ZHANG~2 Xu SHEN~(2,3)Chang-l.n MEI~(4,6)l.-l. FU~4 l.-ping l.N~5 Jian DING~5 Bing XIONG~2 Xi-shan XIONG~4 Hong l.U~(2,6)Xiao-min l.O~(2,6)Hua-l.ang JIANG~(2,3)2 Drug Discovery and Design Centre,State Key l.boratory of Drug Research,Shanghai institute of Materia Medica,Shanghai institutes for Biol.gical.Sciences,Chinese Academy of Sciences,Shanghai 201203.china 3 School.of Pharmacy.East china University of Science and Technol.gy,Shanghai 200237,china 4 Division of Nephrol.gy,Department of Internal.medicine,Changzheng Hospital.Second Mil.tary Medical.University,Shanghai 200003,china 5 Division of Antitumor Pharmacol.gy,Shanghai institute of Materia Medica,Shanghai 201203.china 2Drug Discovery and Design Centre State Key Laboratory of Drug Research Shanghai Institute of Materia Medica Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai 201203 China 2Drug Discovery and Design Centre State Key Laboratory of Drug Research Shanghai Institute of Materia Medica Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai 201203 China 2Drug Discovery and Design Centre State Key Laboratory of Drug Research Shanghai Institute of Materia Medica Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai 201203 China 2Drug Discovery and Design Centre State Key Laboratory of Drug Research Shanghai Institute of Materia Medica Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai 201203 China 3School.of Pharmacy East China University of Science and Technology Shanghai 200237 China 3School.of Pharmacy East China University of Science and Technology Shanghai 200237 China 4Division of Nephrol.gy Department of Internal Medicine Changzheng Hospital Second Military Medical University Shanghai 200003 China 5Division of Antitumor Pharmacol.gy Shanghai Institute of Materia Medica Shanghai 201203 China 5Division of Antitumor Pharmacol.gy Shanghai Institute of Materia Medica Shanghai 201203 China 2Drug Discovery and Design Centre State Key Laboratory of Drug Research Shanghai Institute of Materia Medica Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai 201203 China 4Division of Nephrol.gy Department of Internal Medicine Changzheng Hospital Second Military Medical University Shanghai 200003 China 2Drug Discovery and Design Centre State Key Laboratory of Drug Research Shanghai Institute of Materia Medica Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai 201203 China 2Drug Discovery and Design Centre State Key Laboratory of Drug Research Shanghai Institute of Materia Medica Shanghai Institutes for Bi

Aim:To design and synthesize a novel.cl.ss of protein tyrosine kinase inhibitors,featuring the N-(2-oxo-1,2-dihydroquinol.n-3-yl.methyl.-thiourea ***:First,compounds 1 and 2 were identified using the virtual.screeningapproach in conjunction with binding assay based on surface pl.smon ***,3 regions of compounds 1 and 2 were sel.cted for chemical.*** compounds were characterized potent inhibitory activities toward thehuman l.ng adenocarcinoma cel. l.ne SPAC ***:Forty new compounds(1-2,3a-g,4a-w,and 5a-l.were designed,synthesized and *** com-pounds(1,3e,41,4w,5a,and 5b)were found to show promising inhibitory activityagainst the SPAC1 tumor cel. *** inhibitory activity of compound 5aincreases approximatel. 10 times more than that of the original.compound ***:This study provides a promising new templ.te with potential.antitu-mot activity.

关键词： N-substituted-thiourea derivatives antitumor SPAC1 tyrosine kinase inhibitor virtual.screening