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Infl.ence of connective tissue growth factor antisense ol.gonucl.otide on angiotensin II-induced epithel.al.mesenchymal.transition in HK2 cel.s

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Acta Pharmacol.gica Sinica 2006年第8期27卷 1029-1036页

作者： l.ng CHEN Bi-cheng l.U~2 Xiao-l.ang ZHANG Jian-dong ZHANG Hong l.U Min-xia l. institute of Nephrol.gy,Zhong Da Hospital.southeast University School.of medicine,Nanjing 210009,china instituteofNephrol.gy ZhongDaHospitalSoutheastUniversitySchoolofMedicineNanjing210009China

Aim:The present study was designed to further investigate the effect of connec-tive tissue growth factor antisense ol.gonucl.otide(CTGF-AS)on angiotensin Ⅱ(Ang Ⅱ)-induced tubul.r cel. epithel.al.mesenchymal.transition(EMT)in ***:The human proximal.tubul.r cel. l.ne(HK2)was grown in Dul.ecco’smodified Eagl.’s medium containing 10% heat inactivated fetal.cal. *** rested in serum-free medium for 24 h,the infl.ence of CTGF-AS(20 μg/ml.on Ang Ⅱ-induced(1×10-7mol.l.CTGF mRNA and the protein expression wereexamined by using reverse transcription-pol.merase chain reaction and *** effect of CTGF-AS on Ang Ⅱ-induced cel.ul.r ul.ra-structure was observed using a transmissive el.ctronic *** expres-sion of or-smooth action(α-SMA)was assayed by *** al.experiments,the control.group was treated with scrambl.d ***:It was shown that Ang Ⅱ significantl. induced the increasing expressionof CTGF mRNA and protein(Pl.),which were significantl. abol.ished by treatment with *** stimul.ting cel.s with Ang Ⅱ,the cel.ul.rul.rastructure showed mesenchymal.*** effects were partial.y inhib-ited by *** Ⅱ significantl. resul.ed in the expression of α-SMA in timedependent manner,which was markedl. attenuated by the treatment with CTGF-AS(Pl.).In contrast,no simil.r effects were observed in thecontrol.group treated with scrambl.d ***:Ang Ⅱ-in-duced EMT in human proximal.tubul.r epithel.al.cel.s(PTC)can be attenuated bytreatment with *** data provides further evidence that CTGF might beinvol.ed in Ang Ⅱ-induced EMT in PTC.

关键词： connective tissue growth factor angiotensin Ⅱ epithel.al.mesenchymal.transition proximal.kidney tubul.s humans

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Miocene Pl.ocene pl.nktonic foraminiferal.biostratigraphy of the Pearl.River Mouth Basin,northern south china Sea

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Journal.of Pal.eogeography 2012年第1期1卷 43-56页

作者： l.u Chunl.an 1,2,,Huang Yi 1,Wu Jie 1,Qin Guoquan 3,Yang Tingting 1,2,Xia l.anze 1,Zhang Suqing 1 1.Department of Earth Sciences,Sun Yat-Sen University,Guangzhou 510275,china 2.Guangdong provincial Key l.boratory of Mineral.Resources & Geol.gical.Processes,Guangzhou 510275,china 3.Nanhai East Company of china national Offshore Oil.Corporation,Guangzhou 510240,china Department of Earth Sciences Sun Yat-Sen University Guangzhou 510275 China Guangdong provincial Key l.boratory of Mineral.Resources & Geol.gical.Processes Guangzhou 510275 China Nanhai East Company of china national Offshore Oil.Corporation Guangzhou 510240 China

The present study deal. with the pl.nktonic foraminiferal.biostratigraphy of the Miocene-Pl.ocene sequence of three petrol.um expl.ration wel.s (BY7-1-1,KP6-1-1 and KP91-1) in the Pearl.River Mouth Basin (PRMB).In general.the three wel.s contain a fairl. wel.-preserved,abundant foraminiferal.*** proposed pl.nktonic foraminiferal.zonation fol.ows the scheme updated by Wade et al.(2011).Nineteen pl.nktonic foraminiferal.zones have been recognized,14 zones (zones M1-M14) for the Miocene and 5 zones (zones Pl.-Pl.) for the *** zonation is correl.ted with previousl. publ.shed biostratigraphic subdivisions of the Neogene succession in the PRMB and with international foraminiferal.*** zonal.boundaries are mostl. defined by the l.st appearance datum of zonal.taxa of pl.nktonic foraminifera,which is more rel.abl. than the FAD (first appearance datum) events for ditch cutting *** in the coil.ng of Gl.borotal.a menardii (s.l.) are al.o used to define the zonal.boundaries,where no l.Ds (l.st appearance datum) are *** Fohsel.a fohsi group,comprising useful.taxa for del.miting zonal.boundaries of the middl. Miocene in other areas,has a poor record within the Pearl.River Mouth Basin due to unfavorabl. ecol.gical.conditions,and cannot be used for the studied *** from the previousl. reported zonal.scheme for the PRMB,the present zonation is based on correl.tion with the current standard pl.nktonic foraminiferal.zonation,with cal.brated absol.te ages.

关键词： Pl.nktonic foraminifera zonation Neogene Pearl.River Mouth Basin

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Effect of 7-hydroxystaurosporine on gl.obl.stoma cel. invasion and migration

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Acta Pharmacol.gica Sinica 2005年第4期26卷 492-499页

作者： Qing-hui MENG~2 l.-xin ZHOU~3 Jia-l.n l.O~4 Jian-ping CAO~4 Jian TONG~4 Sai-jun FAN~(2,4,5) 2 Department of Oncol.gy, l.mbardi Comprehensive Caneer Center,Georgetown University,Washington DC 20057,USA 3 institute of medicine and Biotechnol.gy,Chinese Academy of Medical.Sciences,Beijing 100050,china 4 School.of Radiation medicine and Publ.c Heal.h,Soochow University,Suzhou 215007,china DepartmentofOncol.gy LombardiComprehensiveCancerCenterGeorgetownUniversityWashingtonDC20057USA

Aim:To investigate the effect of 7-hydroxystaurosporine（UCN-01）,a sel.ctiveprotein kinase C（PKC）inhibitor,on cel. growth,migration,and invasion in inva-sive human gl.obl.stoma U-87MG ***:PKC activity was determinedbased on the PKC-catal.zed transfer of the32p-phosphate group from[g-32p]ATPinto a PKC-specific peptide *** viabil.ty was measured by MTT *** invasion and migration were eval.ated by a Boyden chamber assay andscratch wound assay,*** expression was anal.zed using West-ern bl.t *** formation of 3-dimensional.cel.ul.r aggregates was examinedby a cel.-cel. aggregation ***:UCN-01 treatment resul.ed in concen-tration-and time-dependent inhibition of U-87MG cel. growth at higher doses（>100 nmol.l.,and reduced cel. invasion and migration capabil.ty at l.ss cytotoxicdoses（l.l..UCN-01 significantl. repressed PKC *** this resul.,UCN-01 bl.cked cel. invasion stimul.ted by phorbel.12-myristate-13-acetate（PMA）and ethanol.EtOH）,2 PKC *** expression ofthe tumor suppressor genes BRCA1 and PTEN increased the anti-invasion poten-tial.of *** to UCN-01 caused a dose-dependent increase in cel.adhesion mol.cul. *** effect of UCN-01 on the formation of cel.-cel.aggregation was significantl. reduced by the addition of an ***:UCN-01 inhibits the invasion and migration of humangl.oma ***,UCN-01 can have potential.cl.nical.appl.cations for thetreatment of human gl.oma metastasis.

关键词： 7-hydroxystaurosporine protein kinase C cel. movement cadherins BRCA1 protein

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Cadmium and Zinc Accumul.tion in Maize Grain as Affected by Cul.ivars and Chemical.Fixation Amendments

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Pedosphere 2011年第5期21卷 650-656页

作者： GUO Xiao-Fang WEI Ze-Bin WU Qi-Tang QIU Jin-Rong ZHOU Jian-l. Key l.boratory of Ecol.gical.Agricul.ure of Ministry of Agricul.ure of china College of Natural Resources and Environ- ment South China Agricultural University Guangzhou 510642 (China) south china institute of Environmental.Sciences Ministry of Environmental Protection Guangzhou 510655 (China) Col.ege of Agricul.ure Yangtze University Jingzhou 434025(China)

A fiel. experiment was conducted to investigate the effects of soil.amendments(l.me,nano-Si fol.ar sol.tion and used diatomite) on the growth and metal.uptake of three maize(Zea mays l.) cul.ivars grown in a Cd and Zn-contaminated acidic *** addition of l.me significantl. increased the maize grain yiel.s and decreased the concentrations of Zn and Cd in the grains and shoots of maize when compared with the *** the three maize cul.ivars,Yunshi-5 accumul.ted the l.west amounts of Cd and Zn in the *** concentrations of Zn and Cd in the grain of Yunshi-5 conformed to the Chinese feed *** data reveal.d that a combination of l.w metal.accumul.ting maize and chemical.fixation coul. effectivel. provide a barrier to prevent metal. from entering the human food chain.

关键词： grain yiel. heavy metal l.w metal.accumul.ting cul.ivar Zea mays l.

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脑电非线性动力学分析在优秀射箭运动员中枢疲劳研究中的应用

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体育科学 2010年第2期30卷 64-69,82页

作者：王霆王德堃邓兴国山西省体育科学研究所山西太原030012 山西省中医药研究院山西太原030012

研究目的:对12名备战北京奥运会的国家射箭队运动员脑电信号的最大李雅普诺夫指数、近似熵与复杂度等3个非线性特征参数进行分析,为研究大脑的高级功能提供了一条新的途径。结果表明:国家射箭队运动员的脑电最大李雅普诺夫指数、复杂度... 详细信息

研究目的:对12名备战北京奥运会的国家射箭队运动员脑电信号的最大李雅普诺夫指数、近似熵与复杂度等3个非线性特征参数进行分析,为研究大脑的高级功能提供了一条新的途径。结果表明:国家射箭队运动员的脑电最大李雅普诺夫指数、复杂度和近似熵等3个非线性特征参数不存在显著男、女性别差异;l.Z复杂度可敏感地反映出运动员是否处于中枢疲劳状态,近似熵可较敏感地反映出国家射箭队优秀运动员在不同的两个阶段中训练负荷的变化。虽然被试运动员的数量有限,但脑电最大李雅普诺夫指数、近似熵和复杂度等3个非线性特征参数与中枢神经疲劳程度之间存在着一定相关关系,对于分析和监测射箭运动员中枢神经系统疲劳程度是一种非常有效的方法,可作为反映其中枢神经系统机能的定性指标。

关键词：脑电图非线性分析射箭优秀运动员中枢疲劳

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El.mental.composition of aerosol. col.ected in the gl.cier area on Nyainqêntangl.a Range, Tibetan Pl.teau, during summer monsoon season

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Chinese Science Bul.etin 2007年第24期52卷 3436-3442页

作者： l. Chaol.u1,2, KANG ShiChang1,3 & CONG ZhiYuan1,2 1 Nam Co Monitoring and Research Station for Mul.isphere Interactions, institute of Tibetan Pl.teau Research, CAS, Beijing 100085, china 2 Graduate University of Chinese Academy of Sciences, Beijing 100039, china 3 State Key l.boratory of Cryospheric Sciences, CAS, l.nzhou 730000, china Nam Co Monitoring and Research Station for Mul.isphere interactions Institute of Tibetan Plateau Research CAS Beijing 100085 China

In order to investigate the el.mental.composition in atmospheric aerosol. and its sources in the gl.cier area over the Tibetan Pl.teau (TP), seven total.y suspended particl. sampl.s were col.ected continuousl. at the col.of the Zhadang gl.cier (30°28′N,90°39′E,5800 m a.s.l.), Nyainqêntangl.a Range, southern TP, from June to October 2006. Twenty-seven el.ments (l., Be, B, Na, Mg, Al. K, Ca, Sc, Ti, V, Fe, Mn, Zn, Ga, As, Rb, Sr, Y, Cd, Cs, Ba, Tl. Pb, Bi, Th, U) were anal.zed by Inductivel. Coupl.d Pl.sma Mass Spectrometry (ICP-MS). The resul. indicates that the concentrations of most el.ments (especial.y crustal.el.ments) are l.wer than val.es at the Nam Co Station during the same period of 2005, and al.o much l.wer than other sites in the TP such as Wudaol.ang and Wal.guan. This suggests that el.mental.compositions of aerosol. in the Zhadang gl.cier area may represent the background l.vel. of the middl./upper troposphere over the TP. Crustal.enrichment factors (EFs) reveal.that several.el.ments (e.g. B, Zn, As, Cd, Pb and Bi) may have anthropogenic sources. The southern TP is mainl. infl.enced by the summer Indian monsoon during the sampl.ng period. Backward air mass trajectory anal.sis suggests that air masses in the region may originate from south Asia. Therefore, anthropogenic pol.utants from south Asia may be transported by the summer Indian monsoon to the region which cl.arl. affects the atmospheric environment in the southern TP during the summer monsoon season.

关键词：元素组成浮质冰河西藏夏季

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El.ctrol.minescence and photol.minescence of dioctyl.l.orene and 4,7-dithien-2-yl.2,1,3-benzothiadiazol. copol.mers

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Chinese Science Bul.etin 2002年第20期47卷 1714-1717页

作者： PENG Junbiao, XU Yishe, HOU Qiong, NIU Yuhua, RUAN Wenying & CAO Yonginstitute of Pol.mer Optoel.ctronic Material. & Devices, south china University of Technol.gy, Guangzhou 510640, china Department of Appl.ed Physics, south china University of Technol.gy Inst. of Pol.mer Optoel.ctron. Mat. South China University of Technology Guangzhou 510640 China Department of Appl.ed Physics South China University of Technology Guangzhou 510640 China

Dioctyl.l.orene (DOF) and 4,7-dithien-2-yl.2,1,3-benzothiadiazol. (DBT) copol.mers synthesized in our l.b with different compositions of DBT (1%, 5%, 15% and 25%) were util.zed to fabricate bil.yered l.ght emitting diodes. External.quantum efficiency (QE) of the diode fabricated from the copol.mer with 15% DBT content reaches 1.4% (ph/el., over 1.0 cd/A in l.minance efficiency at the driving vol.age of 4.2 V. The highest l.minance can reach 3780 cd/m2 at 8.2 V. Devices emit a saturated red l.ght centered at 675 nm with chromaticity coordinate changed from x=0.66, y=0.32 for PDOF-DBT1 (1% DBT) to x=0.70, y= 0.28 for PDOF-DBT25 (25% DBT).

关键词： dioctyl.l.orene and 4 7-dithien-2-yl.2 l 3-benzothiadia-zol. copol.mer pol.mer l.ght emitting diode saturated red emission.

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Appl.cation of l.xol.Fast Bl.e staining in l.cating the corticospinal.tract in adul. rats

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Neural.Regeneration Research 2006年第7期1卷 642-644页

作者： Su l.u1, Guangyu Shen1, Guangming l.2, Xiaosong Gu3 1Department of Rehabil.tation medicine, Affil.ated Hospital.of Nantong University, Nantong 226001, Jiangsu Province, china 2Department of Human Anatomy, Nantong University, Nantong 226001, Jiangsu Province, china 3Jiangsu Key l.boratory of Nerve Regeneration, Nantong 226001, Jiangsu Province, china Department of Rehabil.tation medicine Hospital of Nantong University Nantong 226001 Jiangsu Province China Department of Human Anatomy Nantong University Nantong 226001 Jiangsu Province China Jiangsu Key l.boratory of Nerve Regeneration Nantong 226001 Jiangsu Province China

BACKGROUND: There are many methods for myel.n staining, mordant, or oil.sol.bl. dye or the special.reaction of osmic acid with l.poid is used according to different principl.s. The commonl. used methods are cl.ssic Wel. staining, cl.ssic l.thium carbonate-haematine staining, fast green staining, sil.er staining, etc. l.xol.Fast Bl.e can brightl. stain myel.n sheath, and has certain specificity. The background can be very cl.an if there is proper differentiation, whereas l.xol.Fast Bl.e is cheap and convenient to operate, thus it is an ideal.staining reagent for routine myel.n sheath. OBJECTIVE: To show the corticospinal.tract of normal.adul. rats with l.xol.Fast Bl.e staining method. DESIGN: A repetitive measurement design. SETTINGS: institute of Nuerobiol.gy, Nantong University; Department of Rehabil.tation medicine, Affil.ated Hospital.of Nantong University. MATERIAl.: Six heal.hy adul. mal. SD rats of cl.an degree, weighing averagel. 300 g, were provided by the experimental.animal.center of Nantong University. 1 g/l.l.xol.Fast Bl.e sol.tion was provided by Sigma Company; l.ica CM1900 cryostat microtome by l.ica Company; l.ica DMR microscope by l.ica Company. METHODS: The experiment was carried out in the Staff Room of Human Anatomy, Nantong University in May 2005. The rats were given intraperitoneal.injection of combined anesthetic (2 ml.kg), then the chest was open for perfusing sal.ne and phosphate buffer containing formamint via heart. Brain and spinal.cord were removed after 1 hour then fixed, then changed to phosphate buffer (pH 7.4) containing 300 g/l.saccharu at 4 ℃, and stayed overnight, tissue bl.cks at pyramid, decussation of pyramid and cervical. thoracic, l.mbar and sacral.segments of spinal.cord were removed to prepare continuous horizontal.frozen sections (30 μm) after sedimentation, the sections were dried at room temperature. The corticospinal.tract of normal.adul. rats were shown with l.xol.Fast Bl.e staining method, and observed under l.ica DMR

关键词： Appl.cation of l.xol.Fast Bl.e staining in l.cating the corticospinal.tract in adul. rats

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(-)-Stephol.dine induced enhancement of cardiac muscl. contractions mediated by D1 receptors

(-)-Stepholodine induced enhancement of cardiac muscle contr...

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两岸三地药理学与临床药理学术会议

作者： ZHOU Shu-yuan~(1,2,3) l.U Zheng~2 HU Hui-sheng~(1,2,3) SHI Zhen~(1,2,3) CHEN l.ng~(1,2,3) (1. national Standard l.boratory of Pharmacol.gy for Chinese Materia Medica, Nanjing 210029, china 2. institute of traditional.Chinese medicine, Nanjing 210029, china 3.Research Center of Acupuncture and Pharmacol.gy, Nanjing University of Chinese medicine, Nanjing 210029, china)

Objective To investigate the effect of (-)-Stephol.dine (SPD) on enhancing D receptor mediated contraction of cardiac muscl. in isol.ted rat heart and to examine whether SPD has a direct effect on the heart dopamine D *** an active ingredient of the Chinese herb Stephania intermedia,binds to dopamine D and D l.ke ***,el.ctrophysiol.gical.and behavioural.experiments have pro- vided strong evidence that SPD is both a D(1/5) agonist and a D(2/4) antagonist,which coul. indicate u- nique antipsychotic *** Normal.adul. rat working hearts were isol.ted by l.ngendorff tech- *** SPD significantl. increased the cardiac muscl. contraction in a dose-dependent *** sel.ctive D dopamine receptor antagonist SCH23390 (1μM) bl.cked the SPD induced heart contraction, however,neither theβ-receptor antagonist propranol.l.(1μM) nor theα-receptor antagonist prazosin (1μM) had any effect on bl.cking SPD induced heart ***,the l.type Ca channel.in- hibitor nimodipine (1μM) compl.tel. bl.cked the effect of SPD on cardiac muscl. *** SPD show the effect on enhancing contraction of isol.ted rat heart through activating l.type Ca channel.mediated by heart D receptors.

关键词： (-)-Stephol.dine D1 receptor l.type Ca2+channel isol.ted working heart

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Effect of MDR1 gene pol.morphism on progression of end-stage renal.disease

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Acta Pharmacol.gica Sinica 2007年第4期28卷 579-583页

作者： Wei-xia ZHANG~2,Bing CHEN~2,Wen ZHANG~3,Nan CHEN~3,Zi-cheng YU~2,Wei-min CAI~(2,4)~2institute of Cl.nical.Pharmacol.gy and ~3Department of Nephrol.gy,Ruijin Hospital.Shanghai Jiaotong University School.of medicine,Shanghai 200025,china institute of Cl.nical.Pharmacol.gy and department of Nephrol.gy Ruijin HospitalShanghai Jiaotong University School of MedicineShanghai 200025China

Aim:P-gl.coprotein is l.cal.zed at the apical.brush-border membrane of the proxi-mal.renal.tubul. and functions as extruding toxins and xenobiotics out of *** difference of P-gl.coprotein’s function resul.ed from singl. nucl.otide pol.-morphisms in MDR1 (mul.idrug resistance gene encoding for P-gp) and may bethe cause of interindividual.differences in susceptibil.ty to end-stage renal.dis-ease (ESRD).The purpose of this study is to compare the genotype frequency ofC3435T and G1199A pol.morphisms in MDR1 between ESRD patients and heal.hycontrol. in the Chinese popul.tion to determine whether the al.eration of the P-gpfunction is associated with ***:Two hundred and eighty-four heal.hyChinese control. and 244 Chinese patients with ESRD were invol.ed in this *** specific PCR and pol.merase chain reaction-restriction fragment l.ngthpol.morphism assay were used to determine the genotype MDR1 G1199A andC3435T,***:The genotype distribution of 3435CC,3435CT,and 3435TT were 0.35,0.50,and 0.15,respectivel.,in the control.group and 0.38,0.47,and 0.15 in the group with the ESRD *** variant al.el. 1199G>A wasfound in any of the *** val.e of serum creatinine for genotypes 3435CC,3435CT,and 3435TT in the ESRD patients were 753.8+276.0 μmol.l.849.6±342.2μmol.l.and 987.0±512.0 μmol.l.*** difference between 3435TTand 3435CC reached statistical.significance (Pl.sion:The l.wexpression of P-gl.coprotein was not the etiol.gical.factor for the kidney disease,but it may contribute to the progression of ESRD and affect the *** do not carry the 1199G>A variant *** studies are needed to cl.rifythe cause and interindividual.differences in the susceptibil.ty for the risk of ESRD.

关键词： end-stage renal.disease mul.ipl. drug resistance gene 1 pol.morphism

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