检索结果-南通市图书馆

Syntheses of amphiphilic biodegradable copolymers of poly(ethyl ethylene phosphate) and poly(3-hydroxybutyrate) for drug delivery

引用

Science China Chemistry 2009年第7期52卷 961-968页

作者： CHENG Jing1.& WANG Jun2 1.Department of Polymer Science and Engineering, University of Science and Technology of China, hefei 230026, China 2 hefei national laboratory for physical sciences at microscale, School of Life sciences, University of Science and Technology of China, hefei 230027, China Department of Polymer Science and Engineering University of Science and Technology of China Hefei 230026 China hefei {1. laboratory for physical sciences at microscale School of Life Sciences University of Science and Technology of China Hefei 230027 China

Biodegradable and amphiphilic triblock copolymers poly(ethyl ethylene phosphate)-poly(3-hydroxybutyrate)-poly(ethyl ethylene phosphate) (PEEP-b-PHB-b-PEEP) have been successfully synthesized through ring-opening polymerization. The structures are confirmed by gel permeation chromatography and NMR analyses. Crystallization investigated by X-ray diffraction reveals that the block copolymer with higher content of poly(ethyl ethylene phosphate) (PEEP) is more amorphous, showing decreased crystallizability. The obtained copolymers self-assemble into biodegradable nanoparticles with a coreshell micellar structure in aqueous solution, verified by the probe-based fluorescence measurements and transmission electronic microscopy (TEM) observation. The hydrophobic poly(3-hydroxybutyrate) (PHB) block serves as the core of the micelles and the micelles are stabilized by the hydrophilic PEEP block. The size and size distribution are related to the compositions of the copolymers. Paclitaxel (PTX) has been encapsulated into the micelles as a model drug and a sustained drug release from the micelles is observed. MTT assay also demonstrates that the block copolymers are biocompatible, rendering these copolymers attractive for drug delivery.

关键词： poly(3-hydroxybutyrate) polyphosphoester drug delivery polymeric nanoparticles

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Short exposure to paclitaxel induces multipolar spindle formation and aneuploidy through promotion of acentrosomal pole assembly

引用

Science China(Life sciences) 2010年第11期53卷 1322-1329页

作者： BIAN MingLei1. FU JingYan1. YAN Yan1. CHEN Qiang1. YANG Chao2, SHI QingHua2,JIANG Qing1.& zhang ChuanMao1.1.OE Key {1. of Cell Proliferation and Differentiation and the State Key {1. of Bio-membrane and Membrane Bio-engineering, College of Life sciences, Peking University, Beijing 1.0871. China 2hefei national {1. for physical sciences at microscale and School of Life sciences, University of Science and Technology of China, hefei 230026, China 1. MOE Key laboratory of Cell Proliferation and Differentiation and the State Key laboratory of Bio-membrane and Membrane Bio-engineering College of Life Sciences Peking University Beijing 100871 China2. Hefei National Laboratory for Physical Sciences at Microscale and School of Life Sciences University of Science and Technology of China Hefei 230026 China

Paclitaxel is a widely used microtubule drug and cancer medicine. Here we report that by short exposure to paclitaxel at a low dose, multipolar spindles were induced in mitotic cells without centrosome amplification. Both TPX2 depletion and Aurora-A overexpression antagonized the multipolarity. Live cell imaging showed that some paclitaxel-treated cells accomplished multipolar cell division and a portion of the daughter cells went on to the next round of mitosis. The surviving cells grew into clones with varied genome content. The results indicated that an aneuploidy population could be induced by short exposure to paclitaxel at a low dose, implicating potential side effects of paclitaxel.

关键词： paclitaxel tpx2 Aurora-A multipolar cell division aneuploidy

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Facile synthesis of dendrimer-like star-branched poly(isopropylacrylamide) via combination of click chemistry and atom transfer radical polymerization

引用

Science China Chemistry 2010年第12期53卷 2520-2527页

作者： WAN XueJuan,XU Jian & LIU ShiYong CAS Key laboratory of Soft Matter Chemistry Department of Polymer Science and Engineering,{1. national laboratory for physical sciences at the microscale,University of Science and Technology of China,{1. 230026,China 1. CAS Key laboratory of Soft Matter Chemistry Department of Polymer Science and Engineering Hefei National Laboratory for Physical Sciences at the Microscale University of Science and Technology of China Hefei 230026 China

We report a facile synthesis method of dendrimer-like star-branched poly(N-isopropylacrylamide) (PNIPAM) via the combination of click chemistry and atom transfer radical polymerization (ATRP) by employing the arm-first ***,the α-azido-ω-chloro-heterodifunctionalized building block,N3-PNIPAM-Cl (G0-Cl),was synthesized via ATRP by 3-azidopropyl 2-chloropropionate as the *** advantage of click chemistry,the first generation (G1. of dendrimer-like star-branched PNIPAM,G1.(Cl)3,was facilely prepared via the click coupling reaction between G0-Cl and *** the construction of second generation (G2) dendrimer-like star-branched PNIPAM,G2-(Cl)6,terminal chloride moieties of G1.(Cl)3 were first converted to azide,and then reacted with excess tripropargylamine to give G1.(alkynyl)6 ;G2-(Cl)6 was subsequently prepared via click reaction between G1.(alkynyl) 6 and *** permeation chromatography (GPC) and matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry were employed to confirm the successful construction of dendrimer-like star-branched *** unique thermal phase transition behavior of this dendrimer-like star-branched polymer in aqueous solutions was further investigated by turbidimetry and micro-differential scanning calorimetry (Micro-DSC).

关键词： dendrimer-like branched ATRP click chemistry

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

p53 and Bad: remote strangers become close friends

引用

Cell Research 2007年第4期17卷 283-285页

作者： Peng Jiang~1.Wenjing Du~1.Mian Wu ~1.0} {1. laboratory for physical sciences at microscale and School of Life sciences,University of Science and Technology of China,Anhui 230027,China hefei{1.laboratoryforphysicalsciencesatmicroscaleandSchoolofLifesciences UniversityofScienceandTechnologyofChinaAnhui230027China

The transcription factor p53 is a key regulator of theDNA damage response to genotoxic stress in higher ***/inactivation of p53 has been implicatedin the pathogenesis of many tumor types[1..p53 respondsto cellular insults,such as DNA damage or hypoxia,eitherby arresting the cell cycle so that DNA damage could berepaired,or by triggering apoptosis if DNA repair is *** activation of apoptosis-related genes byp53 is critical for the induction of programmed cell *** search of target genes of p53 involved in cell-cyclearrest and/or apoptosis,numerous p53-inducible geneshave been identified over the past 1. *** themare genes such as Bax[2],Puma[3],Noxa[4],Bid [5],p21.af1.6],and many *** apoptosis-relatedfactors function in different cell organelles including thecytosolic membrane,the mitochondria and the ***,none of these candidate effectors alone can beaccountable for the complicated mechanisms underlyingthe p53 transcription-dependent apoptotic signaling *** work implicated that Bad may contributeto p53-induced mitochondria-mediated apoptosis,yet noknown molecular mechanism had been ascribed to this

关键词： p53 and Bad gene remote strangers become close friends

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

QCM and SPR Investigations on Enzymatic Degradation of Poly（ε-caprolactone） Film

QCM and SPR Investigations on Enzymatic Degradation of Poly...

引用

第三届工程塑料国际学术研讨会

作者： Yi Hou 1.Jing Chen 1.Peijian Sun 2 Zhihua Gan 2.guangzhao zhang1 1. hefei national laboratory for physical sciences at microscale Department of Chemical Physics University of Science and Technology of China {1. Anhui China 2. CAS Key Laboraotry of Engineering Plastics Institute of Chemistry Chinese Academy of sciences (CAS) Beijing1.0080 China

The enzymatic degradation of poly（ε-caprolactone）（PCL） has been investigated by using quartz crystal microbalance with dissipation （QCM-D） and surface plasmon resonance （SPR） in situ. The frequency and dissipation responses of QCM-D and the SPR signal responses can well describe the weight loss and the morphological changes of a PCL film. Our experiments reveal that the degradation has much dependence on the crystallinity and morphology of PCL. PCL with lower crystallinity （45%） and small spherulites degrades in a way of layer-by-layer. Both QCM-D and SPR measurements demonstrate the degradation is of first-order. However, PCL with higher crystallinity （55%） and larger spherulites exhibits a more complex degradation behavior;namely, the degradation in crystalline region lags behind that in amorphous region, leading to a microporous structure.

关键词：

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Bcl-2 cleavages at two adjacent sites by different caspases promote cisplatin-induced apoptosis

引用

Cell Research 2007年第05期17卷 441-448页

作者： Jianbei Zhu1.Ying Yang1.Jiarui Wu1.2 1.Kev laboratory of Systems Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological {1., Chinese Academy of {1., 320 Yueyang Road, Shanghai 200031. China 2 hefei national laboratory for physical {1. at microscale and School of Life {1., University of Science & Technology of China, hefei 230027, China KeylaboratoryofSystemsBiology InstituteofBiochemistryandCellBiologyShanghaiInstitutesforBiologicalSciencesChineseAcademyofSciences320YueyangRoadShanghai200031China

The protein encoded by bcl-2 proto-oncogene plays an important role in the mitochondria-mediated apoptotic *** the general role of Bcl-2 is anti-apoptotic, previous work showed that Bcl-2 fragments cleaved by caspasescould promote apoptotic process. We report herein that Bcl-2 protein was cleaved to produce two fragments of around23 kDa in human hepatocarcinoma BEL-7404 cells or in Bcl-2 overexpressing CHO cells induced by cisplatin. Treat-ing cells with the general caspase inhibitor z-VAD-fmk blocked the induced cleavage of Bcl-2. Mutagenesis analysesshowed that Bcl-2 was cleaved by caspases at two adjacent recognition sites in the loop domain (YEWD31.AGD34↓V),which could be inhibited by caspase-8 and -3 inhibitors, respectively. Overexpression of the carboxyl terminal 23 kDafragments increased the sensitivity of CHO cells to cisplatin-induced apoptosis. These results indicate that Bcl-2 can becleaved into two close fragments by different caspases during cisplatin-induced apoptosis, both of which contribute tothe acceleration of apoptotic process.

关键词： Bcl-2 apoptosis cisplatin caspase-3 caspase-8

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Nano-size uni-lamellar lipodisq improved in situ auto-phosphorylation analysis of E. coli tyrosine kinase using 1.F nuclear magnetic resonance

引用

Protein & Cell 2015年第3期6卷 229-233页

作者： Dong Li(1. Juan Li(1. Yonglong Zhuang(3) Longhua zhang(1. Ying Xiong(1. Pan Shi (2) Changlin Tian (1.(2) 1.hefei {1. laboratory for physical Science at the microscale & School of Life Science University of Science and Technology of China Hefei 230026 China 3.Department of Chemistry Anhui University Hefei 230027 China 2.High Magnetic Field Laboratory Chinese Academy of Sciences Hefei 230031 China

Dear Editor, Among many of biophysical methods, 1.F NMR spectros- copy has emerged as a powerful tool for characterizing protein structure, dynamics and function properties due to high intrinsic sensitivities of fluorine, 1.0% natural abundance of the NMR-active spin, the absence of any natural background in cells, and exquisite sensitivities of 1.F chemical shift to environment （Shi et al., 201.; Shi et al.,

关键词： Unnatural Amino Acid Interaction Partner Protein Phosphorylation Analysis Amber Stop Codon Membrane Scaffold Protein

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Structures comparison and analysis of human phosphoribosylpyrophosphate synthetase 1.and its different mutations shed light on the possible pathogenic mechanism

Structures comparison and analysis of human phosphoribosylpy...

引用

第十七届国际生物物理大会;第十二届中国生物物理大会

作者： Peng Chen~(1.2),Maikun Teng~(1.2),XuLi~(1.2) 1.hefei {1. laboratory for physical sciences at microscale and School of Life sciences 2.University of Science and Technology of China,hefei,Anhui 230027,People's Republic of China

Human PRS 1.Phosphoribosylpyrophosphate synthetase 1.,an enzyme that catalyzes the reaction between ATP and D-ribose-5-phosphate to produce AMP and PRPP(phosphoribosylpyrophosphate),which is a common substrate of all de novo and salvage synthesis of human purine and pyrimidine nucleotides,links to many human genetic diseases including gout,deafness(reported by our group and our cooperators ) and *** far,1. kinds of different single point mutation of human PRS 1.have been reported to lead to different diseases mentioned *** order to reveal the pathogenic mechanism of different mutation from the structural biology sight,we have solved the 2.0 A wild type human PRS1.crystal structure and 3 kinds of pathogenic mutation crystal *** on the protein structure difference and other in vitro functional experiments,we are trying to find the possible pathogenic mechanism of these different mutations.

关键词： PRS1 Mutation structure

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Biochemical and structural studies of Iml3p-Chl4p complex

Biochemical and structural studies of Iml3p-Chl4p complex

引用

第十七届国际生物物理大会;第十二届中国生物物理大会

作者： Yuyong Tao~(1.2),Xu Li~(1.2),Liwen Niu~(1.2),Maikun Teng~(1.2) 1.hefei {1. laboratory for physical sciences at the microscale and School of Life sciences,University of Science and Technology of China 2.Key laboratory of Structural Biology,Chinese Academy of sciences

Yeast Iml3p and Chl4p were identified as two members of budding yeast outer kinetochore and localized to kinetochore in a Ctf1.p-dependent *** of Iml3p and Chl4p would cause defects in the segregation of minichromosom... 详细信息

关键词： Biochemical and structural studies of Iml3p-Chl4p complex

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Ab Initio study of the reaction of nitric oxide with pre-covered oxygen on Au（1.1. surface

Ab Initio study of the reaction of nitric oxide with pre-cov...

引用

中国化学会第九届全国量子化学学术会议

作者： Wenhua zhang, Jinlong Yang {1. national laboratory for physical sciences at microscale, University of Science and Technology of China, {1., 230026

＜正＞The adsorption and reaction of a single NO molecule with atomic oxygen pre-covered Au（1.1. surface have been investigated theoretically based on the density-functional theory. We studied two kinds of Au（1.1. surfaces with 0.33 ML and 1.ML coverage of oxygen, respectively. The calculated results show that NO reacts with pre-covered oxygen atom to generate NO2 or NO3 spontaneously.

关键词：

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：