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Interferon-α enhances sensitivity of human osteosarcoma U2.S cells to doxorubicin by p53-dependent apoptosis

Acta Pharmacologica Sinica 2007年第11期28卷 1835-1841页

作者： Xiang-wei YUAN~2.Xiao-feng ZHU~(3,5),Xiu-fang HUANG~4.Pu-yi SHENG~2.Ai-shan HE~2.Zi-bo YANG~2.Rong DENG~3,Gong-kanFENG~3,Wei-ming LIAO~(2.5)~2.department of Orthopedic Surgery,The First Affiliated Hospital,{2.,Guangzhou 510080,china ~3 State Key Laboratoryof Oncology in South china,Cancer Center,{2.,Guangzhou 510060,china ~4.department of Pathology,The AffiliatedJiangmen Hospital,{2.,Jiangmen 52.030,china department of Orthopedic Surgery The First Affiliated Hospital Sun Yat-sen University Guangzhou 510080 China State Key Laboratory of Oncology in South china Cancer Center Sun Yat-sen University Guangzhou 510060 China department of Pathology The Affiliated Jiangmen Hospital Sun Yat-sen University Jiangmen 529030 China

Aim:To determine whether interferon-α(IFNα) can enhance doxorubicinsensitivity in osteosarcoma cells and its molecular ***:Cellviability was evaluated using 3-(4.5-dimethylthiazol-2.yl)-2.5-diphenyltetrazoliumbromide *** was studied using Flow cytometry analysis,Hoechst332.8 staining,DNA fragmentation assay,as well as the activation ofcaspase-3 and poly (ADP-ribose) *** expression was detectedby Western *** dependence of p53 was determined using ***:IFNα increased doxorubicin-induced cytotoxicity to a muchgreater degree through apoptosis in human osteosarcoma p53-wild U2.S cells,but not p53-mutant MG63 ***α markedly upregulated p53,Bax,Mdm2.andp2.,downregulated Bcl-2.and activated caspase-3 and PARP cleavage in re-sponse to doxorubicin in U2.S ***,the siRNA-mediated silencing ofp53 significantly reduced the IFNα/doxorubicin combination-induced cytotoxic-ity and PARP ***:IFNα enhances the sensitivity of humanosteosarcoma U2.S cells to doxorubicin by p53-dependent *** propercombination with IFNα and conventional chemotherapeutic agents may be a ra-tional strategy for improving the treatment of osteosarcoma with functional p53.

关键词： Interferon-α doxorubicin apoptosis p53 osteosarcoma

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Linearly time-varying channel estimation and training power allocation for OFDM/MIMO systems using superimposed training

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Science china(information Sciences) 2011年第7期54卷 1456-1470页

作者： ZHANG Han 1,DAI XianHua 2.& Pan DaRu 1 1 School of Physics and Telecommunication Engineering,South china Normal university,Guangzhou 510006,china 2.department of {4. Science and technology,sun yat-sen university,Guangzhou 51002.,china 1. School of Physics and Telecommunication Engineering South China Normal University Guangzhou 510006 China2. Department of Information Science and Technology SUN Yat-sen University Guangzhou 510026 China

We address the problem of estimating the linearly time-varying (LTV) channel of orthogonal frequency division multiplexing (OFDM)/multiple-input multiple-output (MIMO) systems using superimposed training (ST).The LTV channel is modeled by truncated discrete Fourier *** on this model,a two-step approach is adopted to estimate the LTV channel over multiple OFDM *** also present performance analysis of the channel estimation and derive a closed-form expression for the channel estimation *** is shown that the estimation variances,unlike that of the conventional ST-based schemes,approach to a fixed lower-bound as the training length increases,which is directly proportional to information-pilot power *** wireless communication systems with a limited transmission power,we optimize the ST power allocation by maximizing the lower bound of the average channel *** results show that the proposed approach outperforms the frequency-division multiplexed training schemes.

关键词： OFDM/MIMO systems linearly time-varying channel estimation superimposed training training power allocation

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Mining and Integrating Reliable Decision Rules for Imbalanced Cancer Gene Expression Data Sets

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Tsinghua Science and technology 2012年第6期17卷 666-673页

作者： Hualong Yu 1 , Jun Ni 2., Yuanyuan Dan 3 , sen Xu 4.1. School of Computer Science and Engineering, Jiangsu {2. of Science and technology, Zhenjiang 2.2.03, china 2. {4. of Radiology, Carver College of Medicine, The {2. of Iowa, Iowa City, IA 52.4., USA 3. School of Biology and Chemical Engineering, Jiangsu {2. of Science and technology, Zhenjiang 2.2.03, china 4. School of {12. Engineering, Yancheng Institute of technology, Yancheng 2.4.51, china School of Computer Science and Engineering Jiangsu University of Science and Technology Zhenjiang 212003 China|c|

There have been many skewed cancer gene expression datasets in the post-genomic era. Extraction of differential expression genes or construction of decision rules using these skewed datasets by traditional algorithms will seriously underestimate the performance of the minority class, leading to inaccurate diagnosis in clinical trails. This paper presents a skewed gene selection algorithm that introduces a weighted metric into the gene selection procedure. The extracted genes are paired as decision rules to distinguish both classes, with these decision rules then integrated into an ensemble learning framework by majority voting to recognize test examples; thus avoiding tedious data normalization and classifier construction. The mining and integrating of a few reliable decision rules gave higher or at least comparable classification performance than many traditional class imbalance learning algorithms on four benchmark imbalanced cancer gene expression datasets.

关键词： cancer gene expression data class imbalance paired differential expression genes decision ruleensemble learning majority voting

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Perceptual video hashing robust against geometric distortions

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Science china(information Sciences) 2012年第7期55卷 1520-1527页

作者： XIANG ShiJun 1,4.,YANG JianQuan 2.& HUANG JiWu 3 1 School of information Science and {2.,Jinan university,Guangzhou 510632.china 2.Shenzhen Institutes of Advanced {2.,Chinese Academy of Sciences,Shenzhen 518055,china 3 School of information Science and {2.,sun yat-sen university,Guangzhou 5102.5,china 4.State Key Laboratory of information Security,Institute of Software,Chinese Academy of Sciences,Beijing 10004.,china 1. School of information Science and technology Jinan University Guangzhou 510632 China4. State Key Laboratory of Information Security Institute of Software Chinese Academy of Sciences Beijing 100049 China2. Shenzhen Institutes of Advanced Technology Chinese Academy of Sciences Shenzhen 518055 China3. School of Information Science and Technology Sun Yat-sen University Guangzhou 510275 China

In this paper,we propose a robust perceptual hashing algorithm by using video luminance histogram in *** underlying robustness principles are based on three main aspects:1) Since the histogram is independent of position of a pixel,the algorithm is resistant to geometric deformations; 2. the hash is extracted from the spatial Gaussian-filtering low-frequency component for those common video processing operations such as noise corruption,low-pass filtering,lossy compression,etc.; 3) a temporal Gaussian-filtering operation is designed so that the hash is resistant to temporal desynchronization operations,such as frame rate change and *** a result,the hash function is robust to common geometric distortions and video processing *** results show that the proposed hashing strategy can provide satisfactory robustness and uniqueness.

关键词： video hashing geometric distortion histogram Gaussian filtering

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Fast Fourier-Galerkin methods for first-kind logarithmic-kernel integral equations on open arcs

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Science china Mathematics 2010年第1期53卷 1-22页

作者： WANG Bo1,WANG Rui2.& XU YueSheng3,4.1Academy of Mathematics and Systems Science,Chinese Academy of Sciences,Beijing 100190,china 2.chool of {2. Science and Engineering,Graduate university of the Chinese Academy of Sciences,Beijing 100190,china 3{4. of Mathematics,Syracuse university,Syracuse,NY 132.0},USA 4.4. of Scientific Computing and Computer Applications,sun yat-sen university,Guangzhou 5102.5,china 1. Academy of Mathematics and Systems Science Chinese Academy of Sciences Beijing 100190 China2. School of Information Science and Engineering Graduate University of the Chinese Academy of Sciences Beijing 100190 China3. Department of Mathematics Syracuse University Syracuse NY 13244 USA4. Department of Scientific Computing and Computer Applications Sun Yat-sen University Guangzhou 510275 China

We propose a fully discrete fast Fourier-Galerkin method for solving an integral equation of the first kind with a logarithmic kernel on a smooth open arc,which is a reformulation of the Dirichlet problem of the Laplace equation in the *** optimal convergence order and quasi-linear complexity order of the proposed method are established.A precondition is *** this method with an efficient numerical integration algorithm for computing the single-layer potential defined on an open arc,we obtain the solution of the Dirichlet problem on a smooth open arc in the *** examples are presented to confirm the theoretical estimates and to demonstrate the efficiency and accuracy of the proposed method.

关键词： Dirichlet problem open arc singular boundary integral equations Fourier-Galerkin methods logarithmic potentials

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An Aggregation Composition Compensation Method Based on Paired Net

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International Journal of Automation and computing 2012年第5期9卷 530-538页

作者： Xiao-Yong Mei 1,2.Yi-Yan Fan 2.Chang-Qin Huang 3 Ai-Jun Jiang 1 Shi-Xian Li 1 1 School of information Science and technology,{2.,Guangzhou 510006,china 2.School of Computer Science and technology,Hunan university of Arts and Science,Changde 4.5000,china 3 department of Electrical Engineering and Computer Science,university of California,Irvine,CA,92.97,USA 1. School of information Science and technology Sun Yat-sen University Guangzhou 510006 China 2. School of Computer Science and technology Hunan University of Arts and Science Changde 415000 China 3. department of Electrical Engineering and Computer Science University of California Irvine CA 92697 USA

With regard to the failure and cancellation of business logic of web services composition(WSC),this paper propose a novel web services transaction compensation mechanism based on paired net which can dynamically establish agile compensation-triggered process(CSCP-Nets),and satisfy prospective compensation *** related execution semantics of five usual composition compensation patterns based on paired net are analyzed in the situations of successful execution,failure compensation and failure *** net based application of trip reservation process(TRP) shows that it is feasible.

关键词： Web services composition failure handling composition compensation execution semantic analysis paired net

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First-principle study of interaction of H2.and H2. molecules with （ZnO）_n（n=3-6） ring clusters

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Progress in Natural Science:Materials International 2010年第1期20卷 30-37页

作者： Hua-peng CHEN~1,Jian-ning DING~(2.3),Ning-yi YUAN~2.Xiu-qin WANG~2.Cheng-lung CHEN~4.Duan WENG~(1*) 1.department of Materials Science and Engineering,Tsinghua Universtiy,Beijing 100084.china 2.Center for Low-dimensional Materials,Micro-nano Devices and System, Jiangsu Polytechnic university,Changzhou 2.3164.china 3.Key Laboratory of New Energy Source,Changzhou 2.3164.china 4.department of Chemistry,National sun yat-sen university,Kaohsiung 804.4.Taiwan,china [a] department of Materials Science and Engineering Tsinghua Universtiy Beijing 100084 China [b] Center for Low-dimensional Materials Micro-nano Devices and System Jiangsu Polytechnic University Changzhou 213164 China [c] Key Laboratory of New Energy Source Changzhou 213164 China [d] department of Chemistry National Sun Yat-sen University Kaohsiung 80424 Taiwan China

A density functional calculation was performed to investigate the impact of hydrogen and water molecules on zinc oxide clusters（ZnO）n=3-6…X,where X=H2.nd *** calculated binding energies were corrected for the basis set superposition error （BSSE）.The structural parameters and chemical hardness were calculated for the complexes of zinc oxide clusters and guest *** strength values of the interaction between the clusters and the guest molecules were analyzed based on the topological properties of atoms in molecules（AIM）theory of *** stereo electronic interactions inside the ZnO clusters were analyzed in detail using the natural bond orbital（NBO）analysis.

关键词： density functional theory zinc oxide clusters atoms in molecules

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Purification and partial characterizations of coagulant protein Fla from Daboia russelli siamensis (Myanmar) venom

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Acta Pharmacologica Sinica 2007年第10期28卷 1580-1584页

作者： Huan-huan sun~(1,3),Qi CHEN~(2.3),Xi LIN~1,Jia-shu CHEN~(1,4.,Peng-xin QIU~1,Guang-mei YAN~~1 {2. of Pharmacology,Zhongshan Medical College,sun yat-sen university Guangzhou 510080,china ~2.Guangdong Institute forDrug Control,Guangzhou 510000,china department of Pharmacology Zhongshan Medical College Sun Yat-Sen University Guangzhou China Guangdong Institute for Drug Control Guangzhou China

Aim:To purify and characterize the coagulant protein FIa from Daboia russellisiamensis (Myanmar) ***:FIa was purified from Daboia russellisiamensis (Myanmar) venom by ion-exchange chromatography on CM-SephadexC-50,and gel filtration on Sephadex G-75 and a Superdex 75 *** hemo-static activity of FIa was determined by the method of Williams and *** chromogenic substrates were used respectively to determine the activa-tion of factor X and *** fibrinogen-clotting activity of FIa wasdetermined by the method of Gao et *** saline was used as a negativecontrol while factor Xa and thrombin were used as positive controls,***:FIa,a coagulant protein,was achieved by ion-exchange chromatogra-phy and gel filtration with a molecular weight of 34.4.9 and an isoelectric point *** was shown to have strong hemostatic *** hemostatic activity of0.5 mg FIa was equal to that of 1.562. u *** primarily activated factor X,however,had no influence on prothrombin,nor did it cleave or clot ***:FIa is a factor X-activating enzyme,which could activate factor X tofactor Xa,but has no effect on prothrombin and fibrinogen.

关键词： snake venoms Daboia russelli siamensis factor X blood coagulation

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Correlation analysis between loss of heterozygosity at chromosome 18q and prognosis in the stage-Ⅱ colon cancer patients

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Chinese Journal of Cancer 2010年第8期29卷 761-767页

作者： Wei Wang1,2. Yuan-Fang Li1,2. Xiao-Wei sun1,2. Gong Chen1,3, You-Qing Zhan1,2. Chun-Yu Huang1,2. De-sen Wan1,3, Zhi-Zhong Pan1,3, Zhi-Wei Zhou1,2.1 State Key Labortary of Oncology in South {2., Guangzhou, Guangdong 510060, P. R. {2. {4. of Gasrtic and Pancreatic Surgery, sun yat-sen university Cancer Center, Guangzhou, Guangdong 510060, P. R. {2. 3 department of Colorectal Surgery, sun yat-sen university Cancer Center, Guangzhou, Guangdong 510060, P. R. {2. department of Gasrtic and Pancreatic Surgery Sun Yat-sen University Cancer Center State Key Labortary of Oncology in South China Guangdong Guangzhou 510060 department of Colorectal Surgery Sun Yat-sen University Cancer Center State Key Labortary of Oncology in South China Guangdong Guangzhou 510060

Background and Objective: Colorectal cancer is one of the most common malignant cancers in the world. Although the clinicopathologic staging is the golden criterion for the prognosis at present, the optimum prognostic criteria for colorectal cancer should be a combination of the clinicopathologic staging and the molecular markers. However, there are currently no molecular markers available for the prognosis of colorectal cancer. Several tumor-suppressor genes associated with colorectal cancer have been mapped at the 18q2.-2. region. In this study we detected the frequency of loss of heterozygosity (LOH) at chromosome 18q and investigated the relationship between LOH and clinicopathologic features and its prognostic value for patients with stage Ⅱ colon cancer. Methods: A total of 106 samples of tumor tissues and corresponding normal mucosa from patients with sporadic stage-Ⅱ colon cancer were included in this study. All the samples were formalin-fixed and paraffin-embedded. DNA was extracted from tumor tissues and LOH of D18S4.4. D18S55, D18S58, D18S61 and D18S64.at chromosome 18q was analyzed using polymerase chain reaction (PCR), polyacrylamide gel-electrophoresis, and DNA sequencing method. Multivariate analysis for association between LOH and prognosis in colon cancer patients was performed with Cox proportional hazards regression model. Results: The median follow-up time was 68 months. For 106 patients, 5-year survival rate was 83.6%, which was associated with age and gross tumor type (P = 0.011 and 0.034. respectively). Among 102.patients who were eligible for LOH information, the overall frequency of LOH is 4..0% (50/102., and that of LOH at 5 microsatellite loci of D18S4.4. D18S55, D18S58, D18S61, and D18S64.was 30.2. (2./86), 2..4. (18/77), 2..6% (2./70), 35.0% (2./80), and 2..8%(15/72., respectively. The occurrence of LOH was significantly associated with tumor location and histopathologic grade (P = 0.02., 0.016 and 0.005, respectively). LOH was more freq

关键词：大肠癌染色体杂合性预后患者相关分析聚丙烯酰胺凝胶电泳临床病理

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Binding activity of H-Ras is necessary for in vivo inhibition of ASK1 activity

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Cell Research 2004年第2期14卷 148-154页

作者： Jun DU~(1,2.), Shao Hui CAI~(3,4., Zhe SHI~3, Fumihiko NAGASE~1 ~1department of Medical {2., Nagoya university School of Health Sciences, Aichi 4.1-8673, Japan. ~2.chool of Pharmaceutical Science, sun yat-sen university, Guangzhou, 510089, {4.. ~3department of Immunology, Nagoya university Graduate and Faculty School of Medicine, Aichi, 4.6-8550, Japan. ~4.ollege of Pharmacy, Jinan university, Guangzhou, 510632. {4.. 2.chool of Pharmaceutical Science Sun Yat-Sen University Guangzhou 510089 China. 4.ollege of Pharmacy Jinan University Guangzhou 510632 China. 3department of Immunology Nagoya University Graduate and Faculty School of Medicine Aichi 466-8550 Japan. 1department of Medical technology Nagoya University School of Health Sciences Aichi 461-8673 Japan.

H-Ras is well known as one of the essential components of Ras/Raf/MEK/ERK cascade, which is a critical prosurvival signaling mechanism in most eukaryotic cells. Ras targets Raf/MEK/ERK cascade by integrating and transmitting extracellular signals from growth factor receptors to Raf, leading to the propagation of signals to modulate a serious of cellular survival events. Apoptosis signal-regulating kinasel (ASK1) serves as a general mediator of cell death because it is responsive to a variety of death signals. In this study, we found that H-Ras interacted with ASK1 to cause the inhibition of both ASK1 activity and ASK1-induced apoptosis in vivo, which was reversed only partially by addition of RafS62.A, an antagonist of Raf, whereas MEK inhibitor, PD98059, and PI3K inhibitor, LY2.4.02. did not disturb the inhibitory effect of H-Ras on ASK-1-induced apoptosis. Furthermore, by means of immunoprecipitate and kinase assays, we demonstrated that the interaction between H-Ras and ASK1 as well as the inhibition

关键词： H-Ras ASK1 apoptosis binding activity.

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