检索结果-南通市图书馆

The screening and identification of endogenous retrovirus free CEMPs

在线全文

学校读者我要写书评

暂无评论

science china(Life sciences) 2004年第6期47卷 562-566页

作者： LU Quanzhi1,3, HAN Hongbing1,3, LIAN Zhengxing1,3, LI Ning2,3, ZHANG Qingcai1, ZHAO Zili4, PEI Dezhi1, ZHANG Xiaolan1 & WU Changxin1 1. college of animal science and technology, china agricultural university, beijing 100094, china 2. State Key Laboratory of Agrobiotechnology, beijing 100094, china 3. Laboratory of animal genetics and breeding, Ministry of Agriculture, beijing 100094, china 4. Sangzi Hu Pig breeding Farm, Hubei 434010, china Correspondence should be addressed to Lian Zhengxing college of animal science and technology China Agricultural UniversityBeijing 100094China Laboratory of animal genetics and breeding Ministry of AgricultureBeijing 100094China State Key Laboratory of Agrobiotechnology Beijing 100094China Laboratory of animal genetics and breeding Ministry of AgricultureBeijing 100094China college of animal science and technology China Agricultural UniversityBeijing 100094China Sangzi Hu Pig breeding Farm Hubei 434010China

The provirus DNA sequence of porcine endogenous retrovirus (PERV) distributed in the pig genome is the major obstacle that restricts the swine as the organ donors in xenotransplantation, and the copy number of PERV varies greatly among different breeds and individuals. In the experiment, 67 healthy, female Chinese Experimental Mini-Pigs (CEMPs) aged at 3—6 months were selected from the animal Husbandry Station of china agricultural university, the copy number of PERV and types of envelope protein gene (env) were then investigated by means of PCR analysis and Southern blotting. It is showed that the distribution of types of envelope protein gene in Landrace and CEMPs makes little difference, but the proportion of individuals carrying two types of envelope protein gene (env-A and env-B, which is de- noted as env-AB) is much larger than those which carry only one type of envelope protein gene (env-A or env-B). Meanwhile, two endogenous retrovirus free pigs were found for the first time during our research, and the copy number of others is relatively low, which is about 10 to 20. All the results illuminate the genetic diversity of indigenous pig breeds in china and the potential of CEMPs to serve as organ donors in xenotransplantation.

关键词： swine, CEMPs, porcine endogenous retrovirus (PERV), PCR, Southern blot.

Genetic diversity revealed by genomic-SSR and EST-SSR markers among common wheat, spelt and compactum

在线全文

学校读者我要写书评

暂无评论

Progress in Natural science:Materials International 2005年第1期 25-34页

作者： YANG Xinquan, LIU Peng, HAN Zongfu, NI Zhongfu and SUN Qixin*(department of Plant genetics & breeding, State Key Laboratory for Agrobiotechnology, china agricultural university, beijing 100094, china) department of Plant genetics & breeding State Key Laboratory for Agrobiotechnology China Agricultural University Beijing 100094China

In this study, two SSR molecular markers, named genomic-SSR and EST-SSR, are used to measure the genetic diversity among three hexaploid wheat populations, which include 28 common wheat (Triticum aestivum L.), 13 spelt (Triticum spelta L.), and 11 compactum (Triticum compactum Host.). The results show that common wheat has the highest genetic polymorphism, followed by spelt and then compactum. The mean genetic distance between the populations is higher than that within a population, and similar tendency is detected for individual genomes A, B and D. Therefore, spelt and compactum can be used as potential germplasms for wheat breeding, especially for enriching the genetic variation in genome D. As compared with spelt, the genetic diversity between common wheat and compactum is much smaller, indicating a closer consanguine relationship between these two species. Although the polymorphism revealed by EST-SSR is lower than that by genomic-SSR, it can effectively differentiate diverse genotypes as well. Together with our present results, it is concluded that EST-SSR marker is an ideal marker for assessing the genetic diversity in wheat. Meanwhile, the origin and evolution of hexaploid wheat is also analyzed and discussed.

关键词： common wheat spelt compactum genomic-SSR EST-SSR genetic diversity.

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

柔嫩艾美耳球虫裂殖生殖阶段超微结构的研究

畜牧兽医学报 2001年第5期32卷 452-457页

作者：安健汪明孔繁瑶殷佩云北京农学院动物科技系 102206 中国农业大学动物医学院北京100094

利用透射电镜对柔嫩艾美耳球虫 (Eimeriatenella)裂殖生殖阶段的超微结构进行了观察描述。其第二次裂殖生殖方式为外裂殖生殖 ,裂殖子侵入宿主细胞后 ,裂殖子逐渐变圆形成裂殖体 ,此过程中裂殖子的棒状体首先消失 ,此后锥体和极环消失... 详细信息

利用透射电镜对柔嫩艾美耳球虫 (Eimeriatenella)裂殖生殖阶段的超微结构进行了观察描述。其第二次裂殖生殖方式为外裂殖生殖 ,裂殖子侵入宿主细胞后 ,裂殖子逐渐变圆形成裂殖体 ,此过程中裂殖子的棒状体首先消失 ,此后锥体和极环消失、最后微线、淀粉颗粒和折光体消失。裂殖体长大后 ,细胞膜下陷 ,将裂殖体分成几部分 ,随后裂殖体进行核分裂形成多核裂殖体 ,此后在靠近细胞核处细胞膜加厚外突 ,临近部位的细胞膜凹陷 ,同时近突起部位内膜复合体加厚形成极环 ,随着时间的推移逐渐形成幼稚裂殖子 ,幼稚裂殖子后部与残体相连 ,此过程中裂殖子的锥体、极环、微线、棒状体、淀粉颗粒、折光体依次逐渐形成。裂殖子成熟的标志为从残体脱离 ,裂殖子的膜下微管 2 4根纵向贯穿裂殖子直达顶端和极环相连 ,裂殖子内部由锥体、锥体前环 1、锥体前环 2、极环、微线、线粒体、折光体和 3 多个棒状体等组成。

关键词：柔嫩艾美耳球虫超微结构裂殖生殖裂殖子鸡

Chemical composition,in situ degradability and in vitro digestibility of several shrubs from sandy soils

在线全文

学校读者我要写书评

暂无评论

Chemical composition,in situ degradability and in vitro dige...

Intestinal digestibility of protein and amino acid of ruminant feeds with the mobile nylon bag and in vitro digestion technique

The Ⅶ International Symposium on the Nutrition of Herbivores(ISNH-7)

作者： D.M.Xu~(1 2) K.Wang~(1 3) S.J.Guo~2 ~1department of Grassland science college of animal science and technology china agricultural university beijing 100094 P.R.china ~2Ningxia university Yinchuan Ningxia 750021 P.R.china

＜正＞Chemical compositions,in situ degradability and in vitro digestibility at the post-fruiting period of five sand-banding shrubs,which include Caragana intermedia,Caragana korshinskii,Hedysarum scoparium,Hedysarum laeve and Salix psammophylla were *** to nutritive value, the five shrubs can be used as *** them,*** is the first choice as feedstuff for realizing the sustainability of ley fanning and improving the production of livestock.

关键词： shrubs sandy soils chemical composition in situ degradability in vitro digestibility

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Intestinal digestibility of protein and amino acid of rumina...

Effects of rumen-protected tryptophan on growth performance,fibre characteristics,nutrient utilization and plasma essential amino acids in Cashmere goats during the Cashmere slow growth period

The Ⅶ International Symposium on the Nutrition of Herbivores(ISNH-7)

作者： Q.Yue~1 H.J.Yang~(1 3) Ch.Y.Xie~1 X.B.Yao~1 J.Q.Wang~2 ~1department of animal Biology & Physiology college of Biological sciences china agricultural university beijing 100094 P.R.china ~2State Key Laboratory of animal Nutrition Institute of animal science china Academy of agricultural sciences beijing 100094 P.R.china

＜正＞The study was to provide the Chinese Feedbank with intestinal digestibility（Idg）of crude protein（CP）and AA for feedstuffs commonly used in *** various AA of feedstuffs weren’t synchronously digested,while a larger variation was found in the same AA between *** CP Idg estimated by mobile nylon bag technique in soyabean meal,cottonseed meal,peanut cake meal,rapeseed meal,rice bran,maize germ meal,wheat bran and lucerne hay were 0.964,0.813, 0.950,0.826,0.586,0.793,0.679 and 0.400,*** has a strong correlation with the value obtained by three-step in vitro procedure.

关键词： crude protein amino acid intestinal digestibility ruminants

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Effects of rumen-protected tryptophan on growth performance,...

低碳经济与高效养殖——第六次全国饲料营养学术研讨会暨动物营养学分会成立三十周年大会

作者： Hui Ma~a,Wei Zhang~a,Xiaoping Zhu~a,Weihong Song~b,Jun Liu~c,Zhihai Jia~(a,*) a college of animal science and technology,china agricultural university,beijing 100193,P.R.china b department of science and Research of Hei longjiang Academy of Land-reclamation sciences,Jiamusi 154007,P.R.china c The college of animal science and technology of Hei longjiang August First Land-Reclamation university, Daqing 163319,P.R.china * Corresponding Author.Present address:college of animal science and technology,china agricultural university,beijing 100193,P.R.china.

Tryptophan(Trp) is a limiting amino acid in growing ruminants and supplementation of Trp in a rumen-protected form may be positive effects on performance in *** may affect cashmere fibre growth by regulating plasma melatonin *** December to February,identified as the cashmere slow growth period,cashmere growth rate slows down and plasma melatonin secretion ***,Trp supplementation may affect cashmere fibre growth positively during this period.Tryptophan(Trp) is a limiting amino acid in growing ruminants and supplementation of Trp in a rumen-protected form may be positive effects on performance in *** may affect cashmere fibre growth by regulating plasma melatonin *** December to February,identified as the cashmere slow growth period,cashmere growth rate slows down and plasma melatonin secretion ***,Trp supplementation may affect cashmere fibre growth positively during this period.

关键词： rumen-protected tryptophan growth performance fibre characteristics nutrient utilization plasma essential amino acids Cashmere goats

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

植物逆境胁迫耐受性功能基因组研究进展

生物化学与生物物理进展 2001年第6期28卷 797-801页

作者：陈其军王学臣刘强中国农业大学农业部生理生化开放实验室北京100094

为了更加高效地利用基因工程技术提高植物对逆境胁迫的耐受性 ,需要在全基因组水平上对植物逆境胁迫耐受性的复杂机制进行整合性研究 .植物逆境胁迫耐受性功能基因组的研究可概括为 :利用胁迫特异性的表达序列标签 (EST)及cDNA微阵列 (... 详细信息

为了更加高效地利用基因工程技术提高植物对逆境胁迫的耐受性 ,需要在全基因组水平上对植物逆境胁迫耐受性的复杂机制进行整合性研究 .植物逆境胁迫耐受性功能基因组的研究可概括为 :利用胁迫特异性的表达序列标签 (EST)及cDNA微阵列 (或基因芯片 )技术筛选与胁迫相关的候选基因 ,然后利用反向遗传学等技术对候选基因的功能进行研究 ,利用酵母双杂交、正向遗传学等技术对基因及基因产物间的相互关系进行研究 .通过这些研究可以全面地了解植物对胁迫 (渗透、干旱、极端温度 )响应的复杂机制和相互作用以及相应的信号转导途径。

关键词：逆境胁迫功能基因组表达序列标签微阵列反向遗传学植物

体细胞克隆牛和转基因体细胞克隆牛的遗传学分析(英文)

在线全文

学校读者我要写书评

暂无评论

生物化学与生物物理进展 2004年第8期31卷 699-704页

作者：黄银花徐公瑾杜志强胡晓湘张磊涂剑锋程雪波李宁中国农业大学农业与生物技术国家重点实验室北京100094

分析了来自同一细胞系的体细胞克隆牛甜甜、庆庆、浒娃及来源同一培养转基因体细胞系转基因体细胞克隆牛九妹、乐娃和 1个妊娠 8个月转基因流产胎牛 8C2以及随机抽取的 1头鲁西黄牛 (LX)、 1头褐斯坦牛 (HS)在 2 4个微卫星位点标记牛的... 详细信息

分析了来自同一细胞系的体细胞克隆牛甜甜、庆庆、浒娃及来源同一培养转基因体细胞系转基因体细胞克隆牛九妹、乐娃和 1个妊娠 8个月转基因流产胎牛 8C2以及随机抽取的 1头鲁西黄牛 (LX)、 1头褐斯坦牛 (HS)在 2 4个微卫星位点标记牛的基因型 .结果表明 2 4个多态位点均表现出多态 ,等位基因数为 1～ 5个 ,平均为 3 17个 .根据网上公布的数据 ,按其最高频率计算 ,甜甜、庆庆、浒娃、九妹、乐娃、 8C2与培养细胞系、转基因细胞系间匹配概率为 1 17× 10 -3 6,根据本研究观察到的数据计算 ,匹配概率为 1 90× 10 -2 3 ;而与随机抽取的 1头鲁西黄牛及褐斯坦牛的基因型分别在 2 3和2

关键词：体细胞克隆牛转基因遗传学等位基因

Experimental Researches on Carcinogenesis or Tumorigenicity of MDCK Canine Kidney Cell(CKC) Lines and Analysis of Their Chromosome Karyotypes

在线全文

学校读者我要写书评

暂无评论

agricultural sciences in china 2002年第7期1卷 816-827页

作者： ZHANG De-li, LI Liu-jin, XIA Geng-tian, HE Xu-yu, GAO Bu-xian, BAI Xiao-hong, HUANG Gao-sheng, LIU Shang-gao, YAN Long-fei and FANG Fu-de( department of Immunology, School of Basic Medical sciences , Peking university Health science Center , beijing 100083 , P. R . china beijing Institute of Preventive Medicine , beijing 100071 , P. R . china department of Toxicology at Center for Research & Development of animal Healthcare Products , Fourth Military Medical university , Xian 710032 , P. R . china department of Pathology at School of Pre-clinical Education , Section of Pathology at Xi-Jing Hospital, Institute for Cancer Research at china Cancer Research Foundation , Fourth Military Medical university, Xian 710032 , P. R . china Key Open Laboratory of Preventive Veterinary Medicine of State Ministry of Agriculture, college of animal Medical sciences & State Key Laboratory of agricultural Biotechnology , college of Biological sciences , china agricultural university , beijing 100094 , P.R.china State Key Laboratory of Medical Molecular Biology, School of Basic Medical sciences at the Peking Union Medical Colloge /Institute of Basic Medical sciences at the Chinese Academy of Medical sciences , beijing 100005 , P. R . china) department of Immunology School of Basic Medical Sciences Peking University Health Science Center Beijing 100083 P. R.China beijing Institute of Preventive Medicine Beijing 100071P.R.China department of Pathology at School of Pre-clinical Education department of Toxicology at Center for Research Development of animal Healthcare Products Fourth Military Medical University Xian 710032 P. R . China beijing Institute of Preventive Medicine Beijing 100071P.R.China department of Pathology at School of Pre-clinical Education Section of Pathology at Xi-Jing Hospital Institute for Cancer Research at China Cancer Research Foundation Fourth Military Medical University Xian 710032 P. R . China Key Open Laboratory of Preventive Veterinary Medicine of State Ministry of Agriculture College of Animal Medical Sciences State Key Laboratory of Agricultural Biotechnology College of Biological Sciences China Agricultural University Beijing 100094 P State Key Laboratory of Medical Molecular Biology School of Basic Medical Sciences at the Peking Union Medical Colloge/Institute of Basic Medical Sciences at the Chinese Academy of Medical Sciences Beijing 100005 P.R. China

The chromosomal number variations & structural aberrations of the MDCK cell line, primary feline or canine kidney cell(FKC or CKC) and Hela cell line were investigated and their karyotypes of conventional chromosome bands were analyzed. The carcinogenesis or tumorigenicity testing of these cell lines in about 232 nude mice and for colony formation in soft agarose and for haemagglutination under different concentration of plant lectins of these cells were carried out. Under the prerequisite that the incidence of cancer or tumor in negative-control nude mice inoculated subcutaneously with primary feline or canine kidney cell cultures purified in vitro at passage 3 was 0 (0/22) and 0 (0/10), respectively. The incidence of the progressively-growing malignant tumor(MT) in positive-control nude mice inoculated subcutaneously with Hela cell cultures of KB, X, or NM20/X strain was 10/10, 25/25 and 5/51, respectively. The results showed that the incidence of tumor in nude mice with tetraploid YA strain of MDCK cell during 20 - 45 passages, with hy-podiploid JB strain of MDCK cell on passage 25, with di-and hypoploid JC strain of MDCK cell during 2-15 passages or with hypoploid M strain of MDCK cell during 9 - 27 passages was 28/58, 1/5, 4/18 and 0/31, respectively. The chromosomal analysis results showed that the ratio of difference in the rate of modal chromosome number between high(mcs+ n) and lowest (mcs) passages was not more than 5% - 15% and the structure aberrations was generally 0 - 3% . These results proved that the genetic characteristics of chromosomal number of cell lines determines their tumorigenicity, but it is species-specific. MDCK line has tumorigenicity no matter what its chromosome karyotype is, at least it has very low tumorigenicity even when its modal chromosome number is hypoploid. The repeatedly frozen, thawed and split controls of tumorigenicity-positive cell lines(X strain of Hela, M strain of BHK-21, JA strain of Vero, YA strain of MDCK) have much

关键词： Cell line MDCK Hela Feline or canine kidney cell Karyotypes Carcinogenesis or tuniori-genicity