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science Bulletin 2005年第18期 67-72页

作者： SUN Zhaogang1,2 & ZHANG Manfu1 1. Lab of animal Molecular Virology, college of Biological science, china agricultural university, beijing 100094, china 2. department of Bacteriology and Immunology, beijing TB & thoracic Tumor Research Institute, beijing 101149, china

The infectious laryngotracheitis virus (ILTV) glycoprotein G (gG) gene of E3 and Zhonghai strains was cloned, sequenced and compared with the gG gene of other Type I animal herpesviruses. To find the localization and the function of the gG in the infected cells, the 35 kD fusion pro-tein (His-GG) was expressed by inserting the coding region of gG except for the signal peptide into pET30a (+). After puri-fication of the His-GG fusion protein, the rats’ antibody to the His-GG was prepared and purified by using the protein G Sepharose. Results of laser scanning confocal microscopy (LSCM) detection showed that the ILTV gG was in the peri-nuclear region and membrane of chicken embryo liver (CEL) and kidney (CEK) cells, and that the gG accumulated more in the coalescent part than in the other parts of the adjacent CEL or CEK cells. The plaque size and the one-step growth curve tests suggested that the ILTV gG was required for viral growth by cell-to-cell direct infection in tissue-cultured CEL cells.

关键词： glycoprotein G expression localization function.

Recent Advances in Swine Amino Acid Nutrition

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Journal of animal science and Biotechnology 2010年第2期1卷 118-130页

作者： Guoyao Wu department of animal science and Faculty of Nutrition,Texas A&M university,college Station,TX,77843,U.S.A and State Key Laboratory of animal Nutrition,china agricultural university,beijing,100193,china department of animal science and Faculty of Nutrition Texas A8dV1 UniversityCollege StationTX77843U. S. A and State Key Laboratory of animal Nutrition China Agricultural UniversityBeijing100193China

Recent advances in swine protein nutrition are characterized by the development of functional a- mino acids （AA） in regulating fetal and postnatal survival, growth and development. These AA include arginine, glutamine, glutamate, proline, leucine, cyste- ine and tryptophan. Due to limited knowledge on AA nutrition, pork producers have traditionally paid little attention to supplementing the arginine family of AA to swine diets. Results of recent studies indicate that functional AA serve important regulatory functions in nutrient metabolism, protein turnover, and immune function, therefore enhancing efficiency of feed utili- zation by pigs. The underlying mechanisms include activation of nitric oxide, mammalian target of rapam- ycin, gaseous signaling, and AMP-activated protein ki- nase pathways, as well as anti-oxidative function. Di- etary supplementation with arginine, glutamine, pro- line or leucine to weanling piglets enhances theirgrowth performance. Arginine or glutamine is also ef- fective in increasing milk production by lactating sows. Furthermore, supplementing arginine to the diet of pregnant gilts between days 30 and 114 of gestation increases the number of live-born piglets and litter birth-weight. Availability of feed-grade functional AA holds great promise for improving animal health and nutrient utilization in pig production worldwide. Addi- tionally, feedstuffs of animal origin [ e. g. , blood meal （ ring dried ）, feather meal （ hydrolyzed ）, meat and bone meal, porcine protein meal, and poultry by-prod- uct meal （both feed- and petfood-grades） ] are excel- lent and cost-effective sources of both essential and functional AA for formulating balanced swine diets. New knowledge on AA nutrition provides a much needed scientific basis for revising the next edition of swine nutrient requirements.

关键词： functional amino acids production swine nutrition

Optimization of the Process of Gelatin-ceftiofur Sodium Microspheres

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Journal of Wuhan university of technology(Materials science) 2010年第6期25卷 975-978页

作者：郝智慧肖希龙 department of Pharmacology and Toxicology College of Veterinary MedicineChina Agricultural University Qingdao KDN animal Drugs technology Development Research Center

Gelatin microsphere（GMS） was prepared through W/O emulsion chemical-crossline *** best formula was selected by examining its appearance,size,drug carrier and drug dissolution *** experimental results showed that the optimized gelatin microspheres were spherical ball with smooth surface and had well *** average size of blank gelatin microspheres was 15.84 μm,while the loaded microspheres＇average diameter were 33.10 μ*** was also shown that drug loading of microspheres increased with increasing loading capacity,but drug encapsulation efficiency had a trend of climbing up and then *** encapsulation efficiency reached the maximum when the dosage ratio was 2：*** the results show ceftiofur sodium microspheres have sustained release in the PBS buffer of pH7.4.

关键词： gelatin microspheres surface morphology size drug carrier

Erk1/2 mftogen-activated protein kinase pathway Involves in myostatin-reguiated differentiation repression

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Erk1/2 mftogen-activated protein kinase pathway Involves in ...

The Predatory Function of Three Spiders to Two Insect Pests in Rice Within a Multi-species Co-existence System

中国遗传学会七届二次青年研讨会

作者： Wei Yang,Yan Chen,Yong Zhang,Xueyan Wang,Ning Yang,and Dahai Zhu National Laboratory of Medical Molecular Biology,Institute of Basic Medical sciences,Chinese Academy of Medical sciences & Peking Union Medical college,5 Dong Dan San Tiao,beijing 100005,china Molecular and Cellular Developmental Biology Laboratory,Harbin Institute of technology,Harbin 150001,china department of animal genetics and breeding,Coliege of animal science and technology,china agricultural university,BeQing 100094,china

The cytokines of transforming growth factor-beta (TGF-β) and its superfamily members are potent regulators of tumorigenesis and multiple cellular events. Myostatin is a member of TGF-β superfamily and plays a negative role in the control of cell proliferation and differentiation. We now demonstrate that myostatin rapidly activated the extracellular signal-regulated Wnase (Erk)1/2 cascade in C2C12 myoblasts. A more remarkable Erk1/2 activation stimulated by myostatin was observed in differentiating than proliferating cells. The results also showed that Ras was the upstream regulator and participated in myostatin-induced Erk1/2 activation because that expression of a dominant negative Ras prevented myostatin-mediated Erk1/2 activation and proliferation Inhibition. Importantly, the myostatin-suppressed myotube fusion and differentiation marker genes expression were attenuated by blockade of Erk1/2 MAPK pathway through pre-treatment with MEK1 inhibitor PD98059, indicating that myostatin-stimulated activation of Erk1/2 negatively regulates myogenic differentiation. Activin receptor Ⅱ b (ActRⅡb) was previously suggested as the only type Ⅱ membrane receptor triggering myostatin signaling. In this study, by using synthesized siRNAs and dominant negative ActRllb, we demonstrate that myostatin failed to stimulate Erk1/2 phosphoryiation and can not inhibited myoblasts differentiation in the ActRllb knockdown C2C12 cells, indicating that ActRⅡb was required for myostatin-stimulated differentiation suppression. Altogether, our findings in this report provide the first evidence to reveal functional role of the Erk1/2 MAPK pathway in myostatin action as a negative regulator of muscle cell growth.

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agricultural sciences in china 2002年第4期1卷 391-396页

作者： LI Jian-quan, SHEN Zuo-rui and ZHAO Zhi-mo( college of Plant Protection , china agricultural university, beijing 100094 , P.R. china department of Plant Protection , South-west agricultural university, Chongqing 400716 , P.R. china) college of Plant Protection China Agricultural University Beijing 100094 P. R. China department of Plant Protection Southwest Agricultural University Chongqing 400716 P. R . China

The prey-seeking behavior of three spiders (X1-Pirata subpiraticus, X2-Clubiona japonicola and X3-Tetragnatha japonica) for brown plant hopper (X4-Nilaparvata lugens) and rice spittle bug (X5-Cal-litettix versicolor) was investigated, as well as how interference between and within species occurred, by using a quadratic regression rotational composite design. Six predation models derived from the analysis of interactions among and within predators and preys were developed. The total predatory capacity of spiders on rice insect pests after coexistence for one day can be expressed as follows: Y3 = 32.795 + 2.25X1 + 1.083X2 + 0.5X3 + 10.167X4 + 3.167X5 - 1.67X12 - 2.42X22 - 3.295X32 - 0.045X42 + 0.455X52 - 3.125X1X2 + 0.375X1X3 -0.625X1X4 - 0.375X1X5 + 0.375X2X3 - 0.875X2X4 + 0.125X2X5 + 0.375X3X4 - 0.375X3X5 + 0.125X4X5. The principal efficiency analysis using this model indicated that increases in insect pest density significantly increased predation by predators; this was much greater than the effect of any single predator. X4 had a greater effect than X5; however, X4 and X5 demonstrated little interspecific interference and even promoted each other and increased predation rates as the densities of the two pests increased. Among the three predators, an increase in the density of X, had the greatest effect on the increase in predation, X3 had the second, X2 the third greatest effect. As predator density increased inter- and intra-species interference occurred, which were largely related to the size, activity, niche breadth, niche overlap and searching efficiency of the predators. X2 produced the greatest interference between different individuals and between any other predator species. X3 had the second greatest, which reduced predation levels at high predator densities. Because of these factors, the highest predation rate was obtained at a prey density of 120 per 4 rice-hills. The optimal proportion of the three predators in the multi-predator prey system was X1: X2:

关键词： Spiders Predation Bio-control Intra-species relationships Co-existence system Interaction Intraspecific competition Interspecific competition animal behavior Natural enemies Rice insect pest

Genomic DNA methylation patterns in bovine preim-plantation embryos derived from in vitro fertilization

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science china(Life sciences) 2007年第1期50卷 56-61页

作者： HOU Jian, LIU Lei, LEI TingHua, CUI XiuHong, AN XiaoRong & CHEN YongFu State Key Laboratory for Agrobiotechnology and department of animal Physiology, college of Biological science, china agricultural university, beijing 100094, china State Key Laboratory for Agrobiotechnology and department of animal Physiology College of Biological Science China Agricultural University Beijing 100094 China

By using the approach of immunofluorescence staining with an antibody against 5-methylcytosine (5MeC), the present study detected the DNA methylation patterns of bovine zygotes and preimplanta-tion embryos derived from oocyte in vitro maturation (IVM), in vitro fertilization (IVF) and embryo in vitro culture (IVC). The results showed that: a) paternal-specific demethylation occurred in 61.5% of the examined zygotes, while 34.6% of them showed no demethylation; b) decreased methylation level was observed after the 8-cell stage and persisted through the morula stage, however methylation levels were different between blastomeres within the same embryos; c) at the blastocyst stage, the methyla-tion level was very low in inner cell mass, but high in trophectoderm cells. The present study suggests, at least partly, that IVM/IVF/IVC may have effects on DNA methylation reprogramming of bovine zygotes and early embryos.

关键词： DNA methylation embryo antibody against 5-methylcytosine in vitro fertilization bovine

Forage, crop and tree residues integrated with herbivore production in some Asian regions

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Forage, crop and tree residues integrated with herbivore pro...

Mapping of Fertility Restoring Gene for Aegilops kotschyi Cytoplasmic Male Sterility in Wheat Using SSR Markers

第七届草食动物营养国际学术研讨会

作者： Q.X.Meng~(1*) D.X.Lu~2 J.X.Liu~3 X.X.Wang~4 L.P.Ren~1 ~1 college of animal science and technology china agricultural university beijing 100094 china ~2 Inner Mongolian Academy of animal science Inner Mongolian Hohhot 010030 china ~3 college of animal science Zhejiang university Hangzhou 310029 china ~4 department of animal science and Veterinary Medicine beijing university of Agriculture beijing 102206 china

＜正＞In this review paper,forage,crop and tree cropping integrated with herbivore industry in agro-forestry systems of Asian countries is critically *** most Asian developing countries,herbivore production accounts for the leading position in overall livestock *** feed their herbivores mainly by locally available feed resources,such as natural tree leaves（oil palm fronds, shrubs shoot）,forages and by-products or residues from vegetables or crops （rice,maize,wheat,sorghum,etc.）.Thus,several integration farming systems, covering various ecological and environmental conditions in Asian regions,are naturally formed,including the integration system using crop residues as fibrous feeds in china,the agro-by-products-buffaloes system in Vietnam,the shrub-livestock system in northern dry lands of china,the oil palm-ruminant system in Malaysia,and the herbivore-coconut plantation system in northern Asian *** major components,technical structure,effectiveness of the system accomplishment and the future perspectives,are also discussed.

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agricultural sciences in china 2002年第8期1卷 845-849页

作者： LIU Bao-shen, SUN Qi-xin, GAO Qing-rong, SUN Lan-zhen, XIE Chao-jie, LI Chuan-you, NI Zhong-fu and DOU Bing-de( Agronomy department, Shandong agricultural university, Taian 271018, P.R.china department of Plant genetics and breeding, china agricultural university, beijing 100094 , P. R . china ) Agronomy department Shandong Agricultural University Taian 271018 P. R. China department of Plant genetics and breeding China Agricultural University Beijing 100094 P.R.China department of Plant genetics and breeding China Agricultural University Beijing 100094 P.R.China Agronomy department Shandong Agricultural University Taian 271018 P. R. China

LK783 was found to be a good fertility restorer for K-type male sterility of wheat. Microsatel-lite markers were employed to map the major restoring gene in LK783. Maintainer and restorer DNA pools were established using the extreme sterile and fertile plants among (KJ5418A//911289/LK783)F1 population, respectively. Seventy-nine sets of SSR primers were screened for polymorphism between the two pools, 6 of which were found polymorphic. Linkage analysis showed that Xgwm11, Xgwm18, Xgwm264a and Xgwm273 were linked to the restoring gene in LK783, while Xgwm11, Xgwm18 and Xgwm273 were co-segregated. The distance between the Rf gene in LK783 and the three co-segregated markers was 6.54 ± 4.37 cM, the distance between Rf gene and Xgwm264a was 5. 71 ± 4.10 cM. The four SSR markers were located on chromosome IBS by amplifying the DNA of nulli-tetrasomics and ditelosomics of CS with the 4 sets of primers, indicating that the major restoring gene in LK783 was located on IBS, but the relative location of the gene was different from Rfv1, allelism of the two genes should be further investigated. The breeding for new fertility restorer lines of K-type cytoplasmic male sterility in wheat would be facilitated by using the four polymorphic markers.

关键词： Wheat ( Triticum aestivum) Cytoplasmic male sterility Restoring gene Molecular marker Microsatellite

Influence of PrP 106―126 on expression of laminin and fibronectin in astrocyte

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science Bulletin 2008年第14期 2161-2165页

作者： LI YuRong1,2, GUAN LeLuo3, YANG JianMin1, ZHOU XiangMei1, YIN XiaoMin1 & ZHAO DeMing1 1 National animal Transmissible Spongiform Encephalopathy Laboratory, college of Veterinary Medicine, china agricultural university, beijing 100094, china 2.department of animal science and technology, Hebei agricultural university, Baoding 071001, china 3 department of agricultural, Food and Nutritional science, university of Alberta, Edmonton, Alberta T6G2P5, Canada

Astrogliosis is a hallmark of prion disease, but the metabolic alterations of astrocytes remain poorly documented. A synthetic peptide corresponding to amino acid 106―126 of the human prion protein (PrP) has been shown to be toxic to neurons. In this study, the effects of PrP 106―126 on astrocytes were investigated in vitro. The proliferation of astrocytes was significantly (P 26 (80 μmol/L) from microglia. The expression of laminin (LN) and fibronectin (FN) was examined at both mRNA and protein levels. The results showed that ex- posure of astrocytes to PrP 106―126 enhanced the expression of LN and FN. The increase of FN in astrocyte cultures required cytokines previously released by activated microglia. This study reveals the expression of LN and FN affected by PrP106―126.

关键词： Prion peptide 106―126 (PrP 106―126) astrocyte microglia laminin fibronectin