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science china(Life sciences) 2004年第1期47卷 25-30页

作者： GU Zhiliang, ZHU Dahai, LI Ming, LI Hui, DENG Xuemei & WU ChangxinMolecular and Cellular Developmental Biology Laboratory, Harbin Institute of technology, Harbin 150001, china college of animal science & technology, Northeast agricultural university, Harbin 150030, china National Laboratories for Agribiotechnology, china agricultural university, beijing 100094, china college of animal science & technology, china agricultural university, beijing 100094, china department of Life science and technology, Changshu Institute of technology, Changshu 215500, china department of Biochemistry and Molecular Biology, National Laboratory of Medical Molecular Biology, Peking Union Medical college, beijing 100005, china

Myostatin, a new member of the TGF-p superfamily, is predominantly expressed in skeletal muscle cells and functions as a negative regulator of skeletal muscle growth in animals. Recently, we have reported three single nucleotide polymorphisms (SNPs) in the chicken my-ostatin gene. Herein, we investigate the association of those SNPs with the production traits in a F2 chicken line derived from Broilers crossing to Silky with the least square analysis. The results show that the BB and AA genotypes are strongly associated with abdominal fat weight (AFW), abdominal fat percentage (AFP), and birth weight (BW) (P 2 individuals have significant difference between CC and DD genotypes (P< 0.05). Breast muscle weight (BMW) of EF birds is higher than that of EE birds (P< 0.05). In this report, we present the first genetic evidence to show that chicken myostatin not only plays an important role in controlling skeletal muscle growth and differentiation, but also may be involved in regulation of adipose growth in chicken.

关键词： myostatin gene, SNPs, F2 chicken population, abdominal fat, breast muscle weight.

Molecular mapping of QTLs for root response to phosphorus deficiency at seedling stage in wheat (Triticum aestivum L.)

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Progress in Natural science:Materials International 2007年第10期17卷 1177-1184页

作者： Li Zhenxing, Ni Zhongfu, Peng Huiru, Liu Zhiyong, Nie Xiuling, Xu Shengbao, Liu Gang and Sun Qixin(Key Laboratory of Crop Heterosis and Utilization and State Key Laboratory for Agrobiotechnology, Key Laboratory of Crop Genomics and Genetic Improvement, beijing Key Laboratory of Crop Genetic Improvement, department of Plant genetics & breeding, china agricultural university, beijing 100094, china) Key Laboratory of Crop Heterosis and Utilization Department of Plant Genetics and Breeding China Agricultural University Beijing 100094 China Key Laboratory of Crop Genomics and Genetic Improvement Department of Plant Genetics and Breeding China Agricultural University Beijing 100094 China beijing Key Laboratory of Crop Genetic Improvement Department of Plant Genetics and Breeding China Agricultural University Beijing 100094 China

Phosphorus (P) deficiency in the soil is one of the major abiotic stresses that limit plant growth and crop productivity throughout the world. Development of cultivars with improved P-deficiency tolerance is an efficient strategy for sustainable agriculture. Plant roots play an important role in crop growth and development, especially in nutrient uptake and improvement of P-efficiency. Mapping quantitative trait loci (QTLs) for root traits and their response to low P stress at seedling stage will facilitate the development of P-efficient wheat cultivars. In this study, 30 QTLs (LOD>2.0) were mapped for the three root traits, such as root length, root number and root dry matter under different P supply conditions and their response to P-stress. These QTLs were distributed on 14 chromosomes, with each of the 5 QTLs explaining more than 10% phenotype variance. Analyses showed that root traits and their response to P-deficiency were controlled by different QTLs. In addition, alleles with positive effects were separated on both parents, and wheat cultivars with improved P-efficiency could be developed by accumulating these positive effect alleles together.

关键词： QTLs roots wheat seedling RIL P-deficiency.

Fine Mapping QTLs Affecting Milk Production Traits on BTA6 in Chinese Holstein with SNP Markers

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Journal of Integrative Agriculture 2013年第1期12卷 110-117页

作者： LIU Rui SUN Dong-xiao WANG Ya-chun YU Ying ZHANG Yi CHEN Hui-yong ZHANG Qin ZHANG Sheng-li ZHANG Yuan college of animal science and technology/Key Laboratory of animal genetics and breeding Ministry of Agriculture/National Engineering Laboratory of Animal Genetics/China Agricultural University Sichuan animal science Academy

Our previous studies demonstrated that the region around markers BMS470 and BMS1242 on BTA6 showed a linkage to 305-d milk yield and composition traits in the Chinese Holstein population. We herein focused on such narrow region to fine map milk production QTLs with 15 SNPs across 25 Mb with each SNP in 1 Mb within most regions in a Chinese Holstein population with daughter design. 1 449 Holstein cows and 11 sires were genotyped for such SNPs by using TaqMan probe and RFLP assays. Multipoint linkage analysis across family revealed a QTL affecting milk yield between PPARGC1A C4075T and SLC34A2 T1713C. Meanwhile, within family analysis found three milk yield QTLs （two in CR T60984131G-CEP135 C501T and one in PDLIM5 A106C-OPN T3907, a fat yield QTLin UGDH T1670C-CR T60984131G region, and two protein yield QTLs in TBC1D1 G501C-UGDH T1670C and PPARGC1A C4075T-SLC34A2 T1713C, respectively. Associations between aforementioned significant SNP markers and milk production traits were further implemented. We found significant associations of PPARGC1A C4075T, SLC34A2 T1713C with milk yield （P0.05, P0.01, P0.01）, UGDH T1670C, and CR T60984131G with fat yield （P0.01, P0.01）, and PPARGC1A C4075T, SLC34A2 T1713C, UGDH T1670C and OPN T3907 with protein yield （P0.01, P0.01, P0.01, P0.01）. Our findings implied that QTLs affecting milk production traits on BTA6 were pleictropism or multigenic effect and PPARGC1A and OPN may be the causal mutations behind milk production QTLs on BTA6 in the Chinese Holstein population.

关键词： fine mapping milk production trait SNP BTA6 Chinese Holstein

Analysis of genome-wide gene expression in root of wheat hybrid and its parents using Barleyl GeneChip

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Progress in Natural science:Materials International 2006年第7期16卷 712-720页

作者： ZHANG Yinhong, NI Zhongfu, YAO Yingyin, ZHAO Jun and SUN Qixin (department of Plant genetics & breeding and State Key Laboratory for Agrobiotechnology, Key Laboratory of Crop Genomics and Genetic Improvement, Ministry of Agriculture / beijing Key Laboratory of Crop Genetic Improvement, china agricultural university, beijing 100094, china) china Agr Univ Dept Genet & Plant Breeding Beijing 100094 Peoples R China china Agr Univ Minist AgrBeijing Key Lab Crop Genet Improvement State Key Lab Agrobiotechnol Key Lab Crop Genom & Genet Improvement Beijing 100094 Peoples R China

Previous studies indicated that differential gene expression between hybrids and their parents is responsible for the hct-crosis. In this paper, we report genome-wide gene expression analysis in roots of a highly heterotic inter-specific hybrid 3338/2463 and its parental inbreds using Barley GeneChip. A total of 1187 genes displayed difference in gene expressions between hybrid 3338/2463 and its parents, and they can be clustered into eight differential expression patterns. Further analysis revealed that among these 1187 genes, 975 genes showed high sequence similarity to the GenBank entries, and represented diverse functional categories, such as metabolism, cell growth and maintenance, signal transduction, response to stress, transcription regulation and others. Fourteen genes were selected for RT-PCR analysis and expression patterns of 9 (64. 29% ) genes were confirmed. Remarkably, 380 differentially expressed genes could be mapped on the Chinese Spring deletion bins, and with the number of genes in seven homoeologous groups being 158, 148, 121, 140, 132, 94 and 127 respectively. It is concluded that a combination of systematic identification of differentially expressed genes with comparative mapping would provide further insight into understanding of molecular basis of heterosis.

关键词： GeneChip gene expression heterosis hybrid wheat.

Research of blastocyte-like structure in chicken

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science china(Life sciences) 2005年第5期48卷 481-486页

作者： LI Jia1, PAN Qiuzhen1, LI Junying1, HAN Hongbing1, SUN Shufeng1, YANG Jun1, XU Shuguang, TIAN Liang, LIAN Zhengxing1,2, YANG Ning1,2 & LI Ning2,3 1. college of animal science and technology, china agricultural university, beijing 100094, china 2. Laboratory of animal genetics and breeding, Ministry of Agriculture, beijing 100094, china 3. State Key Laboratory of Agrobiotechnology, beijing 100094, china college of animal science and technology China Agricultural University Beijing 100094 China college of animal science and technology China Agricultural University Beijing 100094 China Laboratory of animal genetics and breeding Ministry of Agriculture Beijing 100094 China Laboratory of animal genetics and breeding Ministry of Agriculture Beijing 100094 China State Key Laboratory of Agrobiotechnology Beijing 100094 China

The chicken embryo is a classic model used to investigate embryonic development, gene expression, and tissue differentiation, and is also an important research tool in studying the animal functional genomics. The whole blastoderms of fresh unincubated eggs from White Leg-horn chickens were collected with a paper ring, mechanically broken into small pieces and cul-tured in medium. Then the small pieces would develop into blastocyte-like structures (BLS), which could be facilitated by an addition of fetal bovine serum (FBS) to the primary culture and their diameter was nearly doubled from 12 to 24 h. The additional yolk had no positive effect on the development in the first 12 h but encouraged the BLSs attaching and inner cells differentiat-ing instead in 24 h. The inner cells of the BLS showing a high alkaline-phosphatase activity similar to those in mouse embryonic stem (ES) cells and also expressing a large amount of the specific stage embryonic antigen-1 (SSEA-1) on the surface, which was known to be the char-acteristic of non-differentiated mouse and avian ES cells, could finally differentiate into nerve-like cells, fibroblast cells and so on in the medium. Leukemia inhibitory factor (LIF) facilitated the cells’ proliferation and prevented differentiation in the suspended culture of the BLSs. So we drew the conclusion that the BLS obtained from broken blastoderm can be used to amplify avian ES cells so as to initiate a new method of producing transgenic chickens.

关键词： BLS, blastocyst-like structure, embryonic stem cells, ES cells, chicken.

Expression of Mucl During Implantation in Sows

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Expression of Mucl During Implantation in Sows

有机镁与有机硒对肉鸡机体组织抗氧化机能的影响

第十五次全国动物遗传育种学术讨论会

作者： REN Qian,FU Jinlian,WANG Aiguo~* college of animal science and technology,china agricultural university/Key Laboratory of animal genetics and breeding Ministry of Agriculture 100193,beijing,P.R.china

＜正＞Introduction Litter size is one of the most economically important traits in pig *** ovulation,fertilization and implantation,till the birth of piglet,every step may affect litter *** these steps,implantation is... 详细信息

关键词： RT-PCR Immunohistrochemistry Implantation endometrium Muc1 sows

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畜牧兽医学报 2003年第6期34卷 542-547页

作者：邱榕生呙于明中国农业大学动物科技学院营养与饲料系北京100094

研究在日粮中使用有机硒和有机镁对肉鸡生长性能和抗氧化机能的影响。试验采用2×2因子设计,共分成4个处理:对照组、有机硒组(添加0 25mg/kg的有机硒)、有机镁组(添加0 2%的有机镁)和复合组(有机硒和有机镁同时使用)。每个处理140只鸡,... 详细信息

研究在日粮中使用有机硒和有机镁对肉鸡生长性能和抗氧化机能的影响。试验采用2×2因子设计,共分成4个处理:对照组、有机硒组(添加0 25mg/kg的有机硒)、有机镁组(添加0 2%的有机镁)和复合组(有机硒和有机镁同时使用)。每个处理140只鸡,共560只鸡。试验结果表明:日粮中使用有机镁可提高肝脏和心脏中过氧化氢酶的活性(P<0 05),增加肝脏中谷胱甘肽的合成量(P<0 01),减少肌肉和肝脏中丙二醛的产生量(P<0 01)。使用有机硒显著提高肉鸡前期(1～3周)的饲料转化率(P<0 05)和生长速度(P<0 01);有机硒可提高血浆中谷胱甘肽过氧化物酶的活性(P<0 05)和肝脏的总抗氧化力(P<0 01),减少肝脏中丙二醛的含量(P<0 05)。有机镁和有机硒的使用对提高机体总抗氧化能力和减少肝脏中丙二醛的数量有显著的交互效应(P<0 01)。

关键词：有机镁有机硒肉鸡机体组织抗氧化机能日粮生长性能肝脏心脏过氧化氢酶谷胱甘肽肌肉丙二醛饲料转化率交互效应

Expressed sequence tag analysis and cloning of trehalose-6-phosphate synthase gene from marine alga Laminaria japonica(Phaeophyta)

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Acta Oceanologica Sinica 2012年第6期31卷 139-148页

作者： XUAN Jinsong 1,4,FENG Yanbin 1,WENG Manli 1,ZHAO Ge 1,SHI Jinfeng 1,YAO Jianting 2,WANG Xiuliang 2,GUO Baotai 3,QIAO Lixian 3,DUAN Delin 2,WANG Bin 1 1 The State Key Laboratory of Plant Genomics,Institute of genetics and Developmental Biology,Chinese Academy of sciences,beijing 100101,china 2 The State Key Laboratory of Marine Biology,Institute of Oceanology,Chinese Academy of sciences,Qingdao 266071,china 3 college of Life sciences,Qingdao agricultural university,Qingdao 266109,china 4 department of Biological science and Engineering,School of Chemical and Biological Engineering,university of science and technology beijing,beijing 100083,china The State Key Laboratory of Plant Genomics Institute of Genetics and Developmental Biology Chinese Academy of Sciences Beijing 100101 China

A high quality cDNA library was constructed from the brown alga Laminaria japonica,with the titer of 1.2×10 5 pfu/*** average insert size of the cDNA library is about 1.6 *** the cDNA library,591 cDNA clones were randomly selected and *** a result,574 EST(expressed sequence tag) sequences were *** of the 574 ESTs were submitted to the dbEST database section of GenBank with the accession numbers from CX942625 to *** cDNA library was screened with a α-32 p labeled 453 bp T P S gene probe,which is a partial sequence yielded from Porphyra *** positive cDNA clones were screened and the sequencing data showed that these four cDNA clones covered majority of *** TPS cDNA *** PCR amplification,sequencing and assembling,the entire ORF(open reading frame) sequence of the T P S gene was obtained,which was named *** encodes a protein containing 908 amino acids with a calculated molecular mass of 101 674 *** LjTPS gene was successfully expressed in *** and *** LjTPS gene has potential application both in plant breeding to stress tolerance and in deciphering the T P S gene function and mechanism to stress tolerance.

关键词： cDNA library comparative study expressed sequence tag(EST) analysis Laminaria japonica trehalose-6-phosphate synthase(TPS) T P S gene

Cloning and characterization of a novel R1-MYB transcription factor in maize

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Progress in Natural science:Materials International 2009年第9期19卷 1089-1096页

作者： Guanqing Jia, Bo Li, Dengfeng Zhang, Tifu Zhang, Zhiyong Li, Jingrui Dai, Shoucai Wang* National Maize Improvement Center of china, Key Laboratory of Crop Genomics and Genetic Improvement of Agriculture Ministry, department of Plant genetics & breeding, china agricultural university, beijing 100193, china National Maize Improvement Center of china Key Laboratory of Crop Genomics and Genetic Improvement of Agriculture Ministry Department of Plant Genetics & Breeding China Agricultural University Beijing 100193 China

R1-MYB transcription regulatory factors play vital roles in transcriptional modification during higher plant metabolism and development. In the present study, an R1-MYB gene was isolated from the maize (Zea mays L.) inbred line C8605-2 based on expressed sequence tags (ESTs), which expressed differentially between the hybrid C8605-2 × W1445 and its two parents in an oligonucleotide microarray. The full-length cDNA, designated as ZmMYBE1 (GeneBank Accession No. FJ024049), consists of 1992 nucleotides and contains a 1296-bp open reading frame. One conserved MYB domain was detected near the N-terminus of the deduced amino acid sequence, where an acidic Ser/Thr-rich area was also observed in the downstream region. The sequence of ZmMYBE1, mapped to chromosome 5 (bin 5.03), was revealed belonging to a multi-gene family in the maize genome. Subcellular localization analysis determined that ZmMYBE1 was located in the nucleus. Expression analysis showed that ZmMYBE1 transcripts accumulated in various tissues examined, with high expression levels in the immature ear and low levels in the tassel, root, and stem. The expression analysis for ZmMYBE1 in immature ears showed the highest expression level at the earliest vegetative developmental stage of the plant. Expression of ZmMYBE1 higher than that of the parents was observed in the hybrid C8605-2 × W1445. And also the expression level similar to the lowest of the parents in the hybrid C8605-2 × W245 was observed, revealing different expression patterns for the same gene in various hybrids combined from diversified inbred lines. Transgenic Arabidopsis overexpressing ZmMYBE1 implies that ZmMYBE1 has an important role in developmental regulation in maize.

关键词： MYB transcription factor Differential expression Maize