检索结果-南通市图书馆

成人门急诊急性呼吸道感染诊治与防控专家共识

在线全文

学校读者我要写书评

暂无评论

中华传染病杂志 2024年第6期42卷 321-337页

作者：全球华人临床微生物暨感染学会

急性呼吸道感染在临床中极为常见, 通常病情较轻、预后良好, 但重症患者的住院率和病死率会显著升高。因此, 如何早期识别重症感染、及时诊治与早期预防, 成为重要的临床问题。本共识针对急性呼吸道感染的病原学、流行病学、临床及影像... 详细信息

急性呼吸道感染在临床中极为常见, 通常病情较轻、预后良好, 但重症患者的住院率和病死率会显著升高。因此, 如何早期识别重症感染、及时诊治与早期预防, 成为重要的临床问题。本共识针对急性呼吸道感染的病原学、流行病学、临床及影像学特征、病原检测方法、治疗、预防与控制等方面, 基于循证证据, 总结出23条建议, 供临床参考借鉴, 以提高医师对疾病的认识, 优化诊治及感控策略和门诊就诊流程。

关键词：急性呼吸道感染成人诊治防控专家共识

Bioamendment of petroleum contaminated ultisol:effect on oil content, heavy metals and pH of tropical soil

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Journal of Environmental Sciences 2001年第1期13卷 92-98页

作者： E. D. Udosen 1, J. P. Essien 2, R. M. Ubom 2 (1 department of Chemistry and Biochemistry, university of Uyo, nigeria 2.department of Botany and microbiology, university of Uyo, nigeria) department of Chemistry and Biochemistry University of Uyo Nigeria.

the effect of organic amendments on the oil content, heavy metals concentration and pH of petroleum contaminated sandy loam ultisol obtained from Rumuekpe oil field in Emohua Local Government Area of Rivers State, nigeria was determined. Petroleum contaminated soils were treated with wood ash, compost and sawdust. the addition of organic amendments resulted in a significant(at 95% probability level) decrease in oil content by 92% for composting, 81% for soil treated with sawdust and 58% for soil with ash supplementation, over 6 months. the effect of treatments on the iron(Fe), copper(Cu) and lead(Pb) concentration was significant at P 1. the remediation also affected the pH of soil. this initial pH of 5 6 was depressed by the application of compost and sawdust supplements respectively to a final pH of 5 2 and 5 3. On the other hand, amending the soil with wood ash raised the pH from 5 6 to 6 2. Increased acidity caused a decrease in the heavy metals concentration in the contaminated soil. Soil treatment with compost generally gave the best remediation results, followed by sawdust and then ash. Adjusting the pH of oil contaminated soil to high acidic levels may promote the availability and migration of heavy metals in remediated soils and not necessarily the rate of oil mineralization.

关键词： bioamendament tropical soil nige

Hepatitis C virus infection of human hepatoma cell line 7721 in vitro

在线全文

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2001年第5期7卷 685-689页

作者： Zhi-Qiang Song~1 Fei Hao~1 Feng Min~2 Qiao-Yu Ma~2 Guo-Dong Liu~2.department of Dermatology~1department of infectious diseases~2,Southwest Hospital,third Military Medical university,Chongqing 400038,China department of Dermatology Southwest Hospital Third Military Medical University Chongqing 400038 China. songzq@***

AIM: to establish a cell culture system with long-term replication of hepatitis C virus in vitro. MEtHODS: Human hepatoma cell line 7721 was tested for its susceptibility to HCV by incubating with a serum from a patient with chronic hepatitis C. Cells and supernatant were harvested at various phases during the culturing periods. the presence of HCV RnA, the expression of HCV antigens in cells and/or supernatant were examined by RtPCR, in situ hybridization and immunohistochemistry respectively. RESULtS: the intracellular HCV RnA was first detected on d2 after infection and then could be intermittently detected in both cells and supernatant over a period of at least three months. the expression of HCV nS3,CP10 antigens could be observed in cells. the fresh cells could be infected by supernatant from cultured infected cells and the transmission of viral genome from HCV-infected 7721 cells to PBMCs was also observed. COnCLUSIOn: the hepatoma line 7721 is not only susceptible to HCV but also supports its long-term replication in vitro.

关键词： Carcinoma, Hepatocellular Liver neoplasms Antigens, Viral Cell Division Hepacivirus development Hepatitis C Humans In Situ Hybridization In Vitro Phenotype RnA, Viral Research Support, non-U.S. Gov't tumor Cells, Cultured Virus Replication

Phylogenetic clusters of rhizobia revealed by genome structures

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Science China(Life Sciences) 2004年第3期47卷 268-278页

作者： ZHEnG Junfang1, LIU Guirong1,2, ZHU Wanfu1, ZHOU Yuguang3 & LIU Shulin1,2 1. department of microbiology, School of Basic Medical Sciences, Peking university, Beijing 100083, China 2. department of microbiology and infectious diseases, university of calgary, calgary, ABt2. 4n1, canada 3. Institute of microbiology, Chinese Academy of Sciences, Beijing 100080, China 1. department of microbiology School of Basic Medical Sciences Peking University 100083 Beijing China 2. department of microbiology and infectious diseases University of Calgary ABT2N 4N1 Calgary Canada 3. Institute of microbiology Chinese Academy of Sciences 100080 Beijing China

Rhizobia, bacteria that fix atmospheric nitrogen, are important agricultural resources. In order to establish the evolutionary relationships among rhizobia isolated from different geo-graphic regions and different plant hosts for systematic studies, we evaluated the use of physical structure of the rhizobial genomes as a phylogenetic marker to categorize these bacteria. In this work, we analyzed the features of genome structures of 64 rhizobial strains. these rhizobial strains were divided into 21 phylogenetic clusters according to the features of genome structures evaluated by the endonuclease I-CeuI. these clusters were supported by 16S rRnA compari-sons and genomic sequences of four rhizobial strains, but they are largely different from those based on the current taxonomic scheme (except 16S rRnA).

关键词： rhizobia, phylogeny, genome structure.

Distribution of actinomycetes in oil contaminated ultisols of the niger Delta(nigeria)

在线全文

学校读者我要写书评

暂无评论

Journal of Environmental Sciences 2000年第3期12卷 296-302页

作者： J.P. Essien 1, E. D. Udosen 2 (1. department of microbiology, university of Uyo, nigeria 2. department of Chemistry and Biochemistry, university of Uyo,nigeria) department of microbiology University of Uyo Nigeria department of Chemistry and Biochemistry University of Uyo Nigeria

the distribution of actinomycetes in oil contaminated sandy loam ultisols of the niger Delta region of nigeria was studied to aid in understanding the effect of hydrocarbons on indigenous microbial population in tropical soils. the average total counts of actinomycetes in all the oil samples analysed was 10 3 cfu/g. Higher counts of actinomycetes were observed during the dry season than during the wet season. the counts of hydrocarbonoclastic actinomycetes correlated positively with the total count of actinomycetes. the actinomycetes were generally restricted to the top soil(0—10 cm soil depth) although a seemingly deeper(down to 40 cm soil depth) distribution was noticed in the dry season. the isolates included oil degrading species of Actinoplanes, norcadia, Streptomyces and Streptosporangium. their high oil utilization ability indicates their positive potential and role in the bioremediation of oil spilled soils.

关键词： actinomycetes oil contaminated ultisols

Polarization and Apoptosis of t Cell Subsets in Idiopathic thrombocytopenic Purpura

在线全文

学校读者我要写书评

暂无评论

Cellular & Molecular Immunology 2005年第5期2卷 387-392页

作者： Fang Liu~(1,2) Changlin WU~1 Xiaomeng Yang~1 Hong Xiao~1 Xuemin Zhuo~1 Zhengwei Cheng~1 Qun Chen~1 ~1department of microbiology and Immunology,Guangdong Medical College,Zhanjiang,Guangdong 524023,China ~2department of microbiology and Immunology,Guangdong Medical College,no.2 Wen-ming East Road,Zhanjiang,Guangdong 524023,China. department of microbiology and Immunology Guangdong Medical College Zhanjiang China. liufang@***

It is well-known that idiopathic thrombocytopenic purpura （ItP） is an acquired organ-specific autoimmune hemorrhagic disease and dysfunctional cellular immunity is considered important in the pathophysiology of ItP. However, polarization patterns and apoptosis profiles of t lymphocytes remain unclear. In this study, we investigated the polarization of t cell subsets, the expressions of apoptotic proteins Fas/FasL on the subsets and the level of anti-apoptotic gene bcl-2 and bax mRnA. It was demonstrated that the ratios of thl/th2 and tcl/tc2 in ItP children were increased obviously and that the average percentages were increased clearly for thl and th2, but not for tcl and tc2. In ItP children, the enhancing expressions were detected for FasL on thl and tcl and for Fas on th2 and tc2. With increasing level of bcl-2 mRnA and decreasing expression of bax mRnA in ItP children, the ratio of bcl-2/bax mRnA was improved obviously, which was positive correlated with the ratio of thl/th2. taken together, our findings indicate that ItP is a thl predominant disease. this polarization pattern of t cell subsets might be related to the high ratio of bcl-2/bax mRnA and the abnormal expressions of Fas and FasL on t cell subsets.

关键词： idiopathic thrombocytopenic purpura polarization apoptosis t cell subset

Arbuscular mycorrhizal for-mation of crucifer leaf mus-tard induced by flavonoids apigenin and daidzein

在线全文

学校读者我要写书评

暂无评论

Chinese Science Bulletin 2004年第12期49卷 1254-1261页

作者： DOnG Changjin1,2 & ZHAO Bin1 1. State Key Laboratory of Agricultural microbiology, Huazhong Agri- cultural university, Wuhan 430070, China 2. department of Biology, Hubei normal university, Huangshi 435002, China 1. State Key Laboratory of Agricultural microbiology Huazhong Agricultural University 430070 Wuhan China 2. department of Biology Hubei Normal University 435002 Huangshi China

Flavonoids from legume root secretion may probably act as signal molecules for expression of Rhizobial “nod” nodulation genes and AM fungal symbiotic gene. Leaf mustard is a non-mycorrhizal plant; it does not contain fla- vonoids and other signal molecules. AM fungi could not in- fect the roots of leaf mustard and form a symbiont in nature, when it was treated with flavonoids (apigenin or daidzein). the results of trypan blue staining showed that two kinds of AM fungi (G. intraradices and G. mosseae) successfully in- fected the roots of non-mycorrhizal plant leaf mustard. AM fungi grew towards and colonized the roots of leaf mustard, producing young spores and completing the course of life. AM fungi are the only one kind of fungi with ALP activity. the result of ALP staining has also proved that AM fungi infected successfully the roots of leaf mustard. AM fungi (G. intraradices and G. mosseae) that existed in the roots of non-mycorrhizal plant leaf mustard were probed by nested PCR and special molecular probes. the above-mentioned proof chains have fully proved that flavonoids induced AM fungi (G. intraradices and G. mosseae) to infect non-my- corrhizal plant and establish symbiotic relationship.

关键词：非菌根植物免疫十字花科叶芥菜芹甙元大豆黄酮类黄酮灌木菌根真菌嵌套PCR trypan蓝色染色

Isoform of Vascular Endothelial Cell Growth Inhibitor (VEGI72-251) Increases Interleukin-2 Production by Activation of t Lymphocytes

在线全文

学校读者我要写书评

暂无评论

Acta Biochimica et Biophysica Sinica 2006年第4期38卷 249-253页

作者： Jing-Juan YAO~1 Min ZHAnG~1 Xiao-Hui MIAO~2 Ping ZHAO~1 Shi-Ying ZHU~1 Hui DInG~1 Zhong-tian QI~1 1 Key Laboratory for Medical microbiology,PLA,department of microbiology,Second Military Medical university,Shanghai 200433,China 2.department of infectious diseases,Changzheng Hospital,Second Military Medical university Shanghai 200433,China KeyLaboratoryforMedicalmicrobiology PLADepartmentofMicrobiologySecondMilitaryMedicalUniversityShanghai200433China departmentofinfectiousdiseases ChangzhengHospitalSecondMilitaryMedicalUniversityShanghai200433China

the objective of this study is to investigate the characteristics of the recombinant variant ofhuman vascular endothelial cell growth inhibitor,VEGI72-251,and compare its biological activities with that ofits prototype VEGI24-174,the recombinant plasmid containing the variant VEGI72-251gene was constructedand expressed in Escherichia *** effects of the expressed VEGI72-251on cell proliferations were checkedin the human umbilical vein endothelial cell line and certain tumor cell lines （ECV304 and B 16）.the inhibitionof VEGI72-251on angiogenesis was detected in the chorioallantoic membrane of chick *** comparisonwith VEGI24-174,the recombinant human VEGI72-251seems to have no effect on the proliferation of endothelialcells and the angiogenesis of the chorioallantoic membrane in *** enzyme-linked immunosorbent assay-based method was used for the measurement of interleukin-2 （IL-2） production by peripheral blood monocytes（PBMCs） treated with *** were pretreated with VEGI72-251（1.25-12.50μg/ml） for 24 h invitro,and the IL-2 concentration in PBMC medium was increased from 354 pg/ml to 1256 pg/*** can beconcluded that VEGI72-251is able to increase the level of human IL-2 production by the activation of *** from VEGI24-174on anti-angiogenesis,VEGI72-251may serve as an anti-cancer factorthrough its activation of t lymphocytes.

关键词： vascular endothelial cell growth inhibitor biological activity interleukin-2

Chloride channel-dependent copper acquisition of laccase in the basidiomycetous fungus Cryptococcus neoformans

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Science China(Life Sciences) 2010年第1期53卷 125-130页

作者： ZHU ChangWei1,JIAnG nan1,XIAO DongGuang2,PAn Jiao1 & ZHU XuDong1 1 State Key Program of microbiology and department of microbiology,College of Life Sciences,nankai university,tianjin 300071,China 2 tianjin Industrial microbiology Key Lab,College of Bioengineering,tianjin university of Science & technology,tianjin 300457,China 1. State Key Program of microbiology and department of microbiology College of Life Sciences Nankai University Tianjin 300071 China2. Tianjin Industrial Microbiology Key Lab College of Bioengineering Tianjin University of Science & Technology Tianjin 300457 China

the CLC chloride channel gene CLC-A of the pathogen yeast Cryptococcus neoformans was previously reported to be critical for multicopper laccase activity and growth at an elevated *** study reports that copper homeostasis was impaired in the clc-a *** was demonstrated by the substantial decrease of the intracellular quantity of copper under copper-limited growth as determined by flame atomic absorption ***-A is a critical factor in copper homeostasis which is required for copper acquisition of laccase in ***.

关键词： chloride channel laccase copper homeostasis Cryptococcus neoformans