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Further Report on Carcinogenesis or Tumorigenicity ofMDCK Canine Kidney Cell(CKC) Lines and Analysis ofTheir Chromosome Karyotypes

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agricultural Sciences in china 2002年第11期1卷 1293-1301页

作者： ZHANG De-li, XIA Geng-tian, HE Xu-yu, GAO Bu-xian ,BAI Xiao-hong ,LI Liu-jin,HUANG Gao-sheng, LIU Shang-gao, [YEN Lung-fei] and FANG Fu-de(Peking university Health Science center, beijing 100083 ,P. R. china beijing Institute of Preventive Medicine , beijing 100071 , P.R. china center for research and Development of Animal Healthcare Products, Fourth Military Medical university, Xi’an 710032, P.R. china Institute for Cancer research at china Cancer research Foundation, Fourth Military Medical university, Xi’an 710032, P.R. china Key Open Laboratory of Preventive Veterinary Medicine, Ministry of agriculture/china agricultural university , beijing 100094 , P. R. china State Key Laboratory of Medical Molecular Biology , Institute of Basic Medical Sciences , beijing 100005 , P. R. china) Peking university Health Science center Beijing 100083P.R.China beijing Institute of Preventive Medicine Beijing 100071 P.R.China center for research and Development of Animal Healthcare Products Fourth Military Medical University Xi'an 710032 P.R. beijing Institute of Preventive Medicine Beijing 100071 P.R.China center for research and Development of Animal Healthcare Products Fourth Military Medical University Xi'an 710032 P.R.China Institute for Cancer research at china Cancer research Foundation Fourth Military Medical University Xi'an 710032 P.R.China Key Open Laboratory of Preventive Veterinary Medicine Ministry of Agriculture/China Agricultural University Beijing 100094 P.R.China State Key Laboratory of Medical Molecular Biology Institute of Basic Medical Sciences Beijing 100005 P.R.China

Using Hela cell cultures as positive control and primary canine kidney cell (CKC) or feline kidney cell (FKC) cultures purified in vitro on passage 3 as negative control, the tumorigenicity of Madin-Darby canine kidney (MDCK) cells was tested in >273 nude mice, and colony formation in soft agarose and haemag-glutination under different concentration of plant lectins of these cells were carried out at the same time. Subsequently, very low tumorigenicity strains of MDCK line were successfully selected; these were evaluated for the production of canine or feline combination viral vaccines, free of infectious agents, and of known cytoge-netic and tumorigenic. It is thus evident that MDCK cell of M, JB, JC, WB or H strain can be approved as substrate for the preparation of attenuated viral vaccines, but MDCK cell of YA, YB and KA strains can not be approved as substrate for the preparation of attenuated viral vaccines. The heritable character of these cell sub-lines is comparatively stable, and shows little significant difference between passages.

关键词： Kidney cell line Nude mice Carcinogenesis Tumorigenicity Canine kidney cell (CKC) MDCK

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Farmland Information Management and Decision Support System based on GIS

Farmland Information Management and Decision Support System ...

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国际农业工程大会

作者： Zhifang YangArtificial Intelligence research center College of Mechanical and Electric Engineering agricultural university of Hebei Post Code: 071001 Baoding china.Gang LiuKey Laboratory of Modern Precision agriculture System Integration research Ministry of Education (china agricultural university) Post Code: 100083 beijing china

＜正＞ The revolution of information technology offers the opportunity for the development of agricultural modernization. On the basis of the development of the practices of precision agriculture in recent years, this paper introduces the key technologies of the system briefly and explains basic field information which integrates with spatial information storage,

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Transcritption regulation of soybean ribulose-1,5-bisphos-phate carboxylase small sub-unit gene by external factors

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chinese Science Bulletin 2002年第1期47卷 37-43页

作者： HE Chaoying, WANG Weiquan,DONGFANG Yang, ZHANG Jinsong, GAI Junyi& CHEN ShouyiPlant Biotechnology Laboratory, Institute of Genetics, chinese Academy of Sciences, beijing 100101, china National center for Soybean Improvement, Soybean research Inst Plant Biotechnology Laboratory Institute of Genetics Chinese Academy of Sciences Beijing China National center for Soybean Improvement Soybean Research Institute Nanjing Agricultural University Nanjing China

Ribulose-l,5-bisphosphate carboxylase small subunit gene (rbcS) is present with multi-gene family in plant genome. In Glycine max, the rbcS polypeptide (EC4.***.1.39) is encoded by a gene family containing 4-8 members. Three full-length rbcS cDNA clones were isolated and characterized from soybean seedlings, and both of their nucleotide and amino acid sequences showed high similarity. Differential accumulation of the rbcS mRNA was observed among roots, hypocotyls, cotyledons, epicotyls and leaves. The rbcS genes were up-regulated by various external factors such as salicylic acid (SA), salt stress and drought stress. The expression level of rbcS genes after being treated by 2.0 mmol/L SA and0.4% NaCl, respectively, is 2.5-3.0-fold as high as that of control sample. Moreover, soybean rbcS mRNA was accu-mulated with diurnal variation but easily influenced by light and low temperature.

关键词： soybean ribulose-1 5-bisphosphate carboxylase small subunit gene (rbcS) salicylic acid water stress diurnal variation.

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Expression of a Modified Cry1Ie Gene in E. coli and in Transgenic Tobacco Confers Resistance to Corn Borer

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Acta Biochimica et Biophysica Sinica 2004年第4期36卷 309-313页

作者： Yun-Jun LIU, Fu-Ping SONG1, Kang-Lai HE1, Yuan YUAN, Xiao-Xia ZHANG, Peng GAO, Jian-Hua WANG, and Guo-Ying WANG * State Key Laboratory for Agrobiotechnology, National center for Maize Improvement, china agricultural university, beijing 100094, china 1State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, CAAS, beijing 100094, china State Key Laboratory for Agrobiotechnology National Center for Maize Improvement China Agricultural UniversityBeijing 100094 China State Key Laboratory for Biology of Plant Diseases and Insect Pests Institute of Plant ProtectionCAAS Beijing 100094 China

The wild-type Cry1Ie gene from Bacillus thuringiensis was modified for its efficient expression in transgenic plants. Modified Cry1Ie gene (designated as Cry1Iem) was cloned into prokaryotic expression vector pET28b and its expression in E. coli was confirmed by SDS-PAGE analysis. Bioassays using crude expression products in E. coli revealed that Cry1Iem protein had a similar toxicity to corn borer as wild-type Cry1Ie. Cry1Iem gene was then inserted downstream of the maize ubiquitin-1 promoter in plant expression vector p3301. Transgenic tobacco plants carrying Cry1Iem showed insecticidal activity against corn borer.

关键词： Bacillus thuringiensis Cry1Iem transgenic tobacco insect-resistance

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New Thinking on the Definition of Innovation Methods

New Thinking on the Definition of Innovation Methods

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第十一届全国经济管理院校工业技术学研究会

作者： Junjie ZHAO 1,Baofeng CHEN 1,Bingyou LU 2 1.china agricultural university,College of Economics & Management,beijing,china,100083 2.china Rural Technology Development center,beijing,china,100045

Innovation methods are attached more and more universal importance which can promote innovation and increase the efficiency of *** article introduces the domestic and foreign scholars’ different perspectives of innovation methods,and firstly re-defined innovation methods in the broad and narrow sense,and reclassified them in accordance with the contents and its roles in the process of *** will pave the way for the further research of innovation methods theory,and provide a reference for the application and integration research of the innovation methods.

关键词： Innovation methods Definition Classification

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Sampling Survey and Identification of Races of Soybean Cyst Nematode (Heterodera glycines Ichinohe) in Huang-Huai Valleys

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Journal of Integrative agriculture 2006年第8期5卷 615-621页

作者： LU Wei-guo1, 2, GAI Jun-yi1 and LI Wei-dong2 1 Soybean research Institute of Nanjing agricultural university/National center for Soybean Improvement/National Key Laboratory for Crop Genetics and Germplasm Enhancement, Nanjing 210095, P.R.china 2 Institute of Cotton and Oil Crops, Henan Academy of agricultural Sciences, Zhengzhou 450002, P.R.china [a]Soybean research Institute of Nanjing agricultural university/National center for Soybean Improvement/National Key Laboratory for Crop Genetics and Germplasm Enhancement Nanjing 210095 P.R.China[b]Institute of Cotton and Oil Crops Henan Academy of Agricultural Sciences Zhengzhou 450002 P.R.China

Soybean cyst nematode (SCN Heterodera glycines Ichinohe) is one of the most important nationwide soybean diseases in china. A total of 38 soil specimens or locations in the area was sampled and tested for SCN races during 2001-2003 for the inspection of race distribution in Huang-Huai Valleys. A map of race distribution was constructed according to the data from both the present study and the published reports cited. Three areas, namely, the area of southeast to Jinan in Shangdong Province; the area of northern Henan Province and its border region to south of Hebei Province; and the area of Luohe, Zhoukou of Henan Province and Fuyang of Anhui Province mainly infested with Race 1 were identified. Race 4 was predominant in Shanxi Province, beijing and the adjacent area of Henan, Shandong, and Anhui provinces, and the delta of Huanghe River in Shandong Province. Race 2 was mainly found in Liaocheng, Dezhou of Shangdong Province and Shijiazhuang of Hebei Province, and Jiaozuo and Huojia of Henan Province. Race 7 was distributed in the west part of Jiaodong Peninsula of Shandong Province and Kaifeng, Huaxian, Wenxian of Henan Province. Race 5 was found and scattered in Hebei and Henan Province. Race 9 was found in Shangqiu of Henan Province, which was reported for the first time in china. It can be seen that Race 1 and Race 4 were the two predominant races in Huang-Huai Valleys, and that research should focus on developing resistant cultivars of these races. There might exist other races in an area with some predominant races. The race substitution in the past decade was not obviously found, therefore, the results should be meaningful to future breeding for resistance to SCN in Huang-Huai Valleys.

关键词： soybean (Glycine max (L.) Merr.) Huang-Huai Valleys cyst nematode (Heterodera glycines Ichinohe) race

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黄河流域河套灌区灌水管理和地下水利用的影响评估研究-基于GIS技术和MODFLOW的耦合模拟（英文）

黄河流域河套灌区灌水管理和地下水利用的影响评估研究-基于GIS技...

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2009水资源与可持续发展高层论坛

作者： XU Xu~(1,2),HUANG GuanHua~(1,2+),QU ZhongYi~3 1 center for agriculture Water research,china agricultural university,beijing,100083,P.R.china 2.chinese-israeli international center for research and training in agriculture,china agricultural university,beijing,100083,P.R.china 3 Water Conservancy and Civil Engineering College,Inner Mongolia agricultural university,Hohhot, 010018,P.R.china

＜正＞Due to severe water scarcity,water resources used in agricultural sector have been reduced markedly in Hetao Irrigation *** of water-saving practices（WSPs） is required for the sustainable agricultural *** human activities including WSPs and increase of groundwater abstraction can lower down the groundwater table,which

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ANALYSIS AND MONITORING FOR EPIDEMIC SYSTEM AS A BASIS FOR CUCUMBER DOWNY MILDEW WARNING IN GREENHOUSE

ANALYSIS AND MONITORING FOR EPIDEMIC SYSTEM AS A BASIS FOR C...

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第四届智能化农业信息技术国际学术会议

作者： Chunjiang Zhao~~(1 2*) Ming Li~(1 3) Xiao Han~(1 2) Zheng Zhang~(1 2) Yan’an Wang~4 1 National Engineering research center for Information Technology in agriculture beijing 100097 PR china 2 Key Laboratory for Information Technologies in agriculture the Ministry of agriculture beijing 100097 PR china 3 College of Information and Electrical Engineering china agricultural university beijing 100083 PR china 4 College of life science Shandong agricultural university Taian 271018 PR china

＜正＞Plant disease warning is a relative new concept and method system for integrated pest management. The basic of plant disease warning is epidemiology mechanistic and monitoring *** analysis of cucumber downy mildew epidemic has been proposed as the framework and criteria of early warning ***,a wide range of monitoring methods,including computer aids,for epidemic system provide foundation of establishing, operation and evaluation for early warning models. These results and approaches would prove useful in modeling and information system development for cucumber downy mildew warning.

关键词： Cucumber downy mildew greenhouse plant epidemiology monitoring epidemics waning

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A Methodology Framework for Regional Land-use Change Studies based on Landsat TM images: A Case Study in Northeast china

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chinese Journal of Population,Resources and Environment 2004年第4期2卷 3-11页

作者： Deng Xiangzheng , Huang Shumin , Zhan Jinyan & Zhao Tao center for chinese agricultural Policy, chinese Academy of Sciences, beijing 100101 Institute of Geographical Sciences and Natural Resources research, chinese Academy of Sciences, beijing 100101 Anthropology Department, Iowa State university, Ames, Iowa 50010-1050, USA, center for chinese agricultural Policy Chinese Academy of Sciences Beijing 100101 China Institute of Geographical Sciences and Natural Resources research Chinese Academy of Sciences Beijing 100101 China Anthropology Department Iowa State University Ames IA 50010-1050 United States

Land-use change is an important aspect of global environment change. It is, in a sense, the direct result of human activities influencing our physical environment. This paper analyzed the land-use change in Northeast china during 1985-2000 based on Landsat TM images. It divides Northeast china into five land-use zones based on the dynamic degree model of land-use: woodland/grassland-arable land conversion zone, dry land-paddy field conversion zone; urban expansion zone, interlocked zone of farming and pasturing and reclamation and abandon zone. The findings include the obvious increase of cropland area, paddy field and dry land increased by 75 and 276 thousand hm2 respectively; urban areas expanded rapidly, areas of town and rural residence increased by 76.8 thousand hm2; areas of forests and grassland decreased sharply with the amounts of 1399 and 1521 thousand hm2 respectively; areas of water body and unused land increased by 148 and 514 thousand hm2 respectively. This paper also discusses the

关键词： spatial pattern land use land-use change driving force Northeast china

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Structural and functional analysis of denitrification genes in Pseudomonas stutzeri A1501

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Science china(Life Sciences) 2005年第6期48卷 585-592页

作者： YAN Yongliang1,2, YANG Jian2, CHEN Lihong2, YANG Fan2, DONG Jie2, XUE Ying2, XU Xingye2, ZHU Yafang2, YAO Zhijian3, LIN Min4, WANG Yiping5 & JIN Qi1,2 1. College of Biological Sciences, china agricultural university, beijing 100094, china 2. State Key Laboratory for Moleclular Virology and Genetic Engineering, beijing 100176, china 3. National center for Human Genome research, beijing 100176, china 4. Biotechnology research Institute, chinese Academy of agricultural Sciences, beijing 100081, china 5. College of Life Sciences, Peking university, beijing 100871, china 1. College of Biological Sciences China Agricultural University 100094 Beijing China 2. State Key Laboratory for Moleclular Virology and Genetic Engineering 100176 Beijing China 3. National center for Human Genome research 100176 Beijing China 4. Biotechnology research Institute Chinese Academy of Agricultural Sciences 100081 Beijing China 5. College of Life Sciences Peking University 100871 Beijing China

Four gene clusters associated with denitrification were identified in the genome of A1501 strain, nar, nir, nor and nos, including 40 genes totally, which encode proteins for sub-stance transportation, gene regulation and reductases. The three gene clusters, nir, nor and nos are adjacent on chromosome and are far from nar gene cluster. Compared with other denitrifying bacteria, the 40 denitrification genes in A1501 strain compose a complete denitrification catalysis system. In A1501 strain, this system has the following characteristics: (i) only one copy of narK gene is found in nar gene cluster; (ii) a narM gene is present between narK and narG; (iii) two genes, dnarE and orfl are identified at downstream of narX and narL genes, of which dnrE per-haps is a transcriptional factor belonging to FNR family; (iv) there are 16 nir genes in A1501, the most in the known denitrifying bacteria; (v) it is for the first time that norR gene has been found in A1501 and also in Pseudomonas; (vi) nos gene cluster is relatively conservative, with a com-pletely identical composition and arrangement of genome to the reference bacteria strain.

关键词： Pseudomonas stutzeri, denitrification, nitrate reductase, nitrite reductase, nitric oxide reductase, nitrous oxide reductase.

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