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IL-8-induced L-selectin shedding regulates its binding kinetics to PSGL-1

chinese Science Bulletin 2009年第16期54卷 2786-2793页

作者： JIA XiaoLing1,2,3, CHEN Juan1,2 & LONG Mian1,2 1 National Microgravity Laboratory, institute of mechanics, chinese academy of sciences, beijing 100190, china 2.center of biomechanics and bioengineering, institute of mechanics, chinese academy of sciences, beijing 100190, china 3 School of Biological Science and Medical Engineering, Beihang University, beijing 100083, china National Microgravity Laboratory Institute of Mechanics Chinese Academy of Sciences Beijing China center of biomechanics and bioengineering Institute of Mechanics Chinese Academy of Sciences Beijing China School of Biological Science and Medical Engineering Beihang University Beijing China

L-selectin plays a crucial role in inflammation cascade by initiating the tethering and rolling of leukocytes on endothelium wall. While many L-selectin molecules are rapidly shed from the cell surface upon activation, the remaining membrane-anchored L-selectin may still play an important role in regulating leukocyte rolling and adhesion with different binding kinetics. Here we developed an in vitro model to activate Jurkat cells via interlukin-8 (IL-8) and quantified the two-dimensional (2D) binding kinetics, using a micropipette aspiration assay, of membrane-anchored L-selectin to P-selectin glycoprotein ligand 1 (PSGL-1) ligand coupled onto human red blood cells (RBCs). The data indicated that L-selectin shedding reduced the amount of membrane-anchored L-selectin and lowered both its reverse and forward rates. These results suggested that the rolling dynamics of activated leukocytes was determined by two opposite impacts: reducing the surface presentation would enhance the rolling but lowering the kinetic rates would decrease the rolling. This finding provides a new insight into under-standing how L-selectin shedding regulates leukocyte rolling and adhesion.

关键词：结合动力学选择素配体白细胞介素8 Jurkat细胞诱导细胞表面微管吸吮

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2023 Guideline for the management of hypertension in the elderly population in china

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Journal of Geriatric Cardiology 2024年第6期21卷 589-630页

作者： Hypertension Branch of chinese Geriatrics Society,beijing Hypertension Association,National Clinical Research center of the Geriatric Diseases Qi HUA Li FAN Zeng-Wu WANG Jing LI 不详 Xuanwu Hospital Capital Medical UniversityBeijingChina chinese PLA General Hospital BeijingChina Fuwai Hospital Chinese Academy of Medical Sciences and Peking Union Medical CollegeBeijingChina

Preamble Hypertension is one of the most common chronic diseases and the primary risk factor for stroke,myocardial infarction and even cardiovascular *** than half of the elderly population has high blood *** people are a unique group,and strategies for the prevention,diagnosis,evaluation,and treatment of hypertension in elderly individuals differ significantly from those in the general population.

关键词： hypertension prevention diagnosis

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Dynamics of morphological changes for mitochondrial fission and fusion

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Science china(Physics,mechanics & Astronomy) 2010年第4期53卷 680-689页

作者： WANG ShiQi 1,2,4 , FU ChangLiang 1,2 , ZHANG Yan 1,2 , CHEN Quan 3 & LONG Mian 1,2 1 Key Laboratory of Microgravity (National Microgravity Laboratory), beijing 100190, china 2.center for biomechanics and bioengineering, institute of mechanics, chinese academy of sciences, beijing 100190, china 3 State Key Laboratory of Biomembrane and Membrane Biotechnology, institute of Zoology, chinese academy of sciences, beijing 100101, china 4 institute of Vertebrate Paleontology and Paleoanthropology, chinese academy of sciences, beijing 100044, china Key Laboratory of Microgravity (National Microgravity Laboratory) Beijing China center for biomechanics and bioengineering Institute of Mechanics Chinese Academy of Sciences Beijing China institute of Vertebrate Paleontology and Paleoanthropology Chinese Academy of Sciences Beijing China State Key Laboratory of Biomembrane and Membrane Biotechnology Institute of Zoology Chinese Academy of Sciences Beijing China

Mitochondria experience continuous fusion and fission in a living cell, but their dynamics remains poorly quantified. Here a theoretical model was developed, upon a simplified population balance equation (PBE), to predict the morphological changes induced by mitochondrial fission and fusion. Assuming that both fission and fusion events are statistically independent, the survival probability of mitochondria staying in the fission or fusion state was formulated as an exponentially-decayed function with time, which depended on the time-dependent distribution of the mitochondrial volume and the fission and fusion rates. Parametric analysis was done for two typical volume distributions. One was Gamma distribution and the other was Gaussian distribution, derived from the measurements of volume distribution for individual mitochondria in a living cell and purified mitochondria in vitro. The predictions indicated that the survival probability strongly depended on morphological changes of individual mitochondria and was inversely correlated to the fission and fusion rates. This work provided a new insight into quantifying the mitochondrial dynamics via monitoring the evolution of the mitochondrial volume.

关键词： mitochondria morphology fission fusion dynamics modeling

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Markov chain approach to identifying Wiener systems

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Science china(Information sciences) 2012年第5期55卷 1201-1217页

作者： ZHAO WenXiao 1,2 & CHEN HanFu 1,2 1 Key Laboratory of Systems and Control,institute of Systems Science,AMSS,chinese academy of sciences,beijing 100190,china 2 National center for Mathematics and Interdisciplinary sciences,chinese academy of sciences,beijing 100190,china Key Laboratory of Systems and Control Institute of Systems Science AMSS Chinese Academy of Sciences Beijing China National center for Mathematics and Interdisciplinary sciences Chinese Academy of Sciences Beijing China

Identification of the Wiener system composed of an infinite impulse response (IIR) linear subsystem followed by a static nonlinearity is *** recursive estimates for unknown coefficients of the linear subsystem and for the values of the nonlinear function at any fixed points are given by the stochastic approx-imation algorithms with expanding truncations (SAAWET).With the help of properties of the Markov chain connected with the linear subsystem,all estimates derived in the paper are proved to be strongly *** comparison with the existing results on the topic,the method presented in the paper simplifies the convergence analysis and requires weaker conditions.A numerical example is given,and the simulation results are consistent with the theoretical analysis.

关键词： Wiener system recursive identification stochastic approximation Markov chain strong consis-tency

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Observation results of relativistic electrons detected by Fengyun-1 satellite and analysis of relativistic electron enhancement (REE) events

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Science china(Physics,mechanics & Astronomy) 2008年第12期51卷 1947-1956页

作者： YANG XiaoChao1,2↑ & WANG ShiJin1 1 center for Space Science and Applied Research, chinese academy of sciences, beijing 100190, china 2 Graduate University of chinese academy of sciences, beijing 100049, china center for Space Science and Applied Research Chinese Academy of Sciences Beijing China Graduate University of chinese academy of sciences Beijing China

The space particle component detector on Fengyun-1 satellite which works at the sun-synchronous orbit of about 870 km altitude has detected relativistic electrons for a long time. In comparison with the SAMPEX satellite observations during 1999–2004, the relativistic electron data from Fengyun-1 satellite from June 1999 to 2005 are used to analyze the relativistic electron enhancement (REE) events at the low earth orbit, and the possible correlation among REE events at the low earth orbit, high-speed solar wind and geomagnetic storms is discussed. The statistical result presents that 45 REE events are found in total during this time period, and the strong REE events with the maximum daily average flux > 400 cm?2·srt-1·s?1 occur mostly during the transition period from solar maximum to solar minimum. Among these 45 REE events, four strong REE events last a longer time period from 26-to 51-day and correlate closely with high speed solar wind and strong geomagnetic storms. Meanwhile, several strong geomagnetic storms occur continuously before these REE events, and these continuous geomagnetic storms would be an important factor causing these long-lasting strong REE events. The correlation analysis for overall 45 events indicates that the strength of the REE events correlates with the solar wind speed and the strength of the geomagnetic storm, and the correlation for strong REE events is much stronger than that for weak REE events.

关键词： relativistic electron enhancement low earth orbit high-speed solar wind geomagnetic storm

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Research of the Gas-solid Flow Character Based on the DEM Method

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Journal of Thermal Science 2011年第6期20卷 521-526页

作者： Xueyao Wang and Yunhan Xiao Research center for Clean Energy and Power,chinese academy of sciences Key Laboratory of Advanced Energy and Power,chinese academy of sciences (institute of Engineering Thermophysics),beijing,100190,china Research center for Clean Energy and Power Chinese Academy of Sciences Beijing China Key Laboratory of Advanced Energy and Power Chinese Academy of Sciences (Institute of Engineering Thermophysics) Beijing China

Numerical simulation of gas-solid flow behaviors in a rectangular fluidized bed is carried out three dimensionally by the discrete element method (DEM).Euler method and Lagrange method are employed to deal with the gas phase and solid phase *** collided force among particles,striking force between particle and wall,drag force,gravity,Magnus lift force and Saffman lift force are considered when establishing the mathematic ***-sphere model is used to describe the collision of *** addition,the Euler method is also used for modeling the solid phase to compare with the results of *** flow patterns,particle mean velocities,particles' diffusion and pressure drop of the bed under typical operating conditions are *** results show that the DEM method can describe the detailed information among particles,while the Euler-Euler method cannot capture the micro-scale *** matter which method is used,the diffusion of particles increases with the increase of gas *** the gathering and crushing of particles cannot be simulated,so the energy loss of particles' collision cannot be calculated and the diffusion by using the Euler-Euler method is *** addition,it is shown by DEM method,with strengthening of the carrying capacity,more and more particles can be schlepped upward and the dense suspension upflow pattern can be ***,the results given by the Euler-Euler method are not consistent with the real situation.

关键词： DEM gas-solid flow numerical simulation

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Developing a microfluidic-based system to quantify cell capture efficiency

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Science china(Life sciences) 2009年第2期52卷 173-181页

作者： YANG Fan, GAO YuXin, ZHANG Yan, CHEN Juan & LONG Mian National Microgravity Laboratory and center for biomechanics and bioengineering, institute of mechanics, chinese academy of sciences, beijing 100080, china National Microgravity Laboratory and center for biomechanics and bioengineering Institute of Mechanics Chinese Academy of Sciences Beijing China

Micro-fabrication technology has substantial potential for identifying molecular markers expressed on the surfaces of tissue cells and viruses. It has been found in several conceptual prototypes that cells with such markers are able to be captured by their antibodies immobilized on microchannel substrates and unbound cells are flushed out by a driven flow. The feasibility and reliability of such a microflu- idic-based assay, however, remains to be further tested. In the current work, we developed a microflu- idic-based system consisting of a microfluidic chip, an image grabbing unit, data acquisition and analysis software, as well as a supporting base. Specific binding of CD59-expressed or BSA-coupled human red blood cells (RBCs) to anti-CD59 or anti-BSA antibody-immobilized chip surfaces was quan- tified by capture efficiency and by the fraction of bound cells. Impacts of respective flow rate, cell concentration, antibody concentration and site density were tested systematically. The measured data indicated that the assay was robust. The robustness was further confirmed by capture efficiencies measured from an independent ELISA-based cell binding assay. These results demonstrated that the system developed provided a new platform to effectively quantify cellular surface markers effectively, which promoted the potential applications in both biological studies and clinical diagnoses.

关键词： cell surface marker microfluidic capture efficiency

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Unsteady Numerical Simulation of Hot Streak/Blades Interaction and Film Cooling

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Journal of Thermal Science 2010年第5期19卷 402-409页

作者： Weilin Yi,Lucheng Ji and Yunhan Xiao Research center for Energy and Power,chinese academy of sciences,beijing,china Key Laboratory of Advanced Energy and Power,chinese academy of sciences,(institute of Engineering Thermophysics),beijing,china P.O.Box 2706,beijing 100190,china Research center for Energy and Power Chinese Academy of Sciences Beijing China Key Laboratory of Advanced Energy and Power Chinese Academy of Sciences (Institute of Engineering Thermophysics) Beijing China beijing China

Deeply research on management and application of hot streak is an important way to breakthrough technique obstacle of aero engine hot *** method is a useful instrument to investigate the correlative *** the paper developed independently three dimensional unsteady parallel computational code-MpiTurbo based on Fortran 90 and MPI at Linux operating *** unsteady numerical simulation was carried out to investigate impacts of the factors,which included circumferential locations of hot streak and clocking positions of blade rows,on the thermal environment of a 1+1 counter-rotating *** results clearly indicated that clocking positions of hot streak/blade row and blade row/blade row had great influence on the time-averaged temperature distribution of the third blade ***,it can be effective for improving thermal environment of turbine to optimize blade parameters and clocking *** film cooling layout was designed by the repetitious steady simulation based on source term *** the flow structure detail was given by the unsteady simulation.

关键词： hot streak turbine parallel computation film cooling

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Monaural voiced speech segregation based on elaborate harmonic grouping strategies

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Science china(Information sciences) 2011年第12期54卷 2491-2500页

作者： LIU WenJu 1 , ZHANG XueLiang 1 , JIANG Wei 1 , LI Peng 2 & XU Bo 2 1 National Laboratory of Pattern Recognition, institute of Automation, chinese academy of sciences, beijing 100190, china 2 Digital Media Content Technology Research center, institute of Automation, chinese academy of sciences, beijing 100190, china National Laboratory of Pattern Recognition Institute of Automation Chinese Academy of Sciences Beijing China Digital Media Content Technology Research center Institute of Automation Chinese Academy of Sciences Beijing China

In this paper, an enhanced algorithm based on several elaborate harmonic grouping strategies for monaural voiced speech segregation is proposed. Main achievements of the proposed algorithm lie in three aspects. Firstly, the algorithm classifies the time-frequency (T-F) units into resolved and unresolved ones by carrier-to-envelope energy ratio, which leads to more accurate classification results than by cross-channel correlation. Secondly, resolved T-F units are grouped together according to minimum amplitude principle, which has been verified to exist in human perception, as well as the harmonic principle. Finally, "enhanced" envelope autocorrelation function is employed to detect amplitude modulation rates, which helps a lot in reducing half-frequency error in grouping of unresolved units. Systematic evaluation and comparison show that performance of separation is greatly improved by the proposed algorithm. Specifically, signal-to-noise ratio (SNR) is improved by 0.96 dB compared with that of previous method. Besides, our algorithm is also effective in improving the PESQ score and subjective perception score.

关键词： computational auditory scene analysis voiced speech separation harmonistic principle minimum amplitude principle elaborate harmonic grouping strategies

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Strong decays of N~*(1535) in an extended chiral quark model

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Science china(Physics,mechanics & Astronomy) 2009年第10期52卷 1452-1457页

作者： AN ChunSheng1,2,3 & ZOU BingSong1,2,3 1 institute of High Energy Physics,chinese academy of sciences,beijing 100049,china 2 Theoretical Physics center for Science Facilities,chinese academy of sciences,beijing 100049,china 3 Graduate University of chinese academy of sciences,beijing 100049,china institute of High Energy Physics Chinese Academy of Sciences Beijing China Theoretical Physics center for Science Facilities Chinese Academy of Sciences Beijing China Graduate University of chinese academy of sciences Beijing China

The strong decays of the N*(1535) resonance are investigated in an extended chiral quark model by including the low-lying $ qqqq\bar q $ components in addition to the qqq component. The results show that these five-quark components in N*(1535) contribute significantly to the N*(1535) → Nπ and N*(1535) → Nη decays. The contributions to the Nη decay come from both the lowest energy and the next-tolowest energy five-quarks components, while the contributions to the Nπ decay come from only the latter one. Taking these contributions into account, the description for the strong decays of N*(1535) is improved, especially for the puzzling large ratio of the decays to Nη and Nπ.

关键词： N*(1535) strong decays five-quark components

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