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Growth phase-dependent expression of proteins with decreased plant-specific N-glycans and immunogenicity in tobacco BY2 cells

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Science China(Life Sciences) 2009年第8期52卷 739-746页

作者： YIN BoJiao1,2,ZHENG NuoYan1,2,LI Yin1,TANG SanYuan2,LIANG LiMing1 & XIE Qi1,2 1 State Key laboratory of Biocontrol,School of Life Sciences,Sun Yat-sen(Zhongshan) University,Guangzhou 510275,China 2 State Key laboratory of plant Genomics,National Centre for plant Gene Research,Institute of Genetics and Developmental Biology,Chinese Academy of Sciences,Beijing 100101,China State Key laboratory of Biocontrol School of Life Sciences Sun Yat-sen (Zhongshan) University Guangzhou 510275 China State Key laboratory of plant Genomics National Centre for Plant Gene Research Institute of Genetics and Developmental Biology Chinese Academy of Sciences Beijing 100101 China

plants possess some desirable characteristics to synthesize recombinant glycoproteins for pharma-ceutical ***,the mammalian glycoproteins produced in plants are somewhat different from their natural counterparts in terms of *** immunogenicity of plant-specific glyco-epitopes is the major concern in human ***,the distribution of N-glycans in different growth phases of tobacco BY2 cells and their immunogenicity in mice were *** was ob-served that the percentage of β1,2-xylose and α1,3-fucose in proteins of growing cells increased and the corresponding protein extracts caused accelerated immune response in *** on this observation,the recombinant erythropoietin in BY2 cells was expressed and characterized,and Western blot analysis showed that the recombinant erythropoietin contained a relatively small amount of plant-specific glyco-epitopes in the early phase of culture *** study may provide a simple but effective strategy for the production of therapeutic glycoproteins with human-like N-glycan structures in plant hosts to avoid a great allergenic risk.

关键词： BY2 cell,N-glycans,protein expression immunogenicity

The relationship of differential expression of genes in GA biosynthesis and response pathways with heterosis of plant height in a wheat diallel cross

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Chinese Science Bulletin 2009年第16期54卷 3029-3034页

作者： WANG XiuLi1,2, YAO YingYin1,2, PENG HuiRu1,2, ZHANG Yi1,2, LU LaHu1,2, NI ZhongFu1,2 & SUN QiXin1,2 1 State Key laboratory for Agrobiotechnology, Key laboratory of Crop Heterosis and Utilization (MOE), Key laboratory of Crop Genomics and Genetic Improvement (MOA), and Beijing Key laboratory of Crop Genetic Improvement, China Agricultural Univer- sity, Beijing 100193, China 2 National plant Gene Research Centre (Beijing), Beijing 100193, China State Key laboratory for Agrobiotechnology Key Laboratory of Crop Heterosis and Utilization (MOE) Key Laboratory of Crop Genomics and Genetic Improvement (MOA) and Beijing Key Laboratory of Crop Genetic Improvement China Agricultural University Beijing 100193 China National plant Gene Research Centre (Beijing) Beijing 100193 China

Heterosis in internode elongation and plant height is commonly observed in hybrid plants, but the molecular basis for the increased internode elongation in hybrids is unknown. In this study, midparent heterosis in plant height was determined in a wheat diallel cross involving 16 hybrids and 8 parents, and real-time PCR was used to analyze alterations in gene expression between hybrids and parents. Significant heterosis of plant height and the first internode in length were observed for all 16 hybrids, but the magnitude of heterosis was variable for different cross combinations. Analysis revealed that the heterosis of the first internode was significantly correlated to that of plant height (r = 0.56, P plant height. Real-time PCR analysis exhibited that significant difference in heterosis of gene ex- pression was observed among all cross combinations. Moreover, heterosis of the first internode in length was correlated significantly and positively with expression heterosis of KS, GA3ox2-1, GA20ox2, GA20ox1D, GA-MYB and GID1-1, but significantly and negatively with expression heterosis of GAI and GA2ox-1, which is consistent with our recently proposed model of GAs and heterosis in wheat plant height, suggesting the alteration of GA biosynthesis and response pathways might be responsible for the observed heterosis in plant height.

关键词：基因差异表达杂种优势杂交小麦反应途径生物合成遗传算法株高双列

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Scale correlation between vegetation and soil in larch forest, NE China

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Journal of Environmental Sciences 2001年第1期13卷 51-57页

作者： MA Ke ming 1, ZU Yuan gang 2, Michel Godron 3 (1. Reserch Center for Eco Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China. E mail: mkm@*** 2. Open Research laboratory of Forest plant Ecology, Northeast Fo Research Center for Eco-Environmental Sciences Chinese Academy of Sciences Beijing 100085 China. mkm@***

After the analysis on the linages of species, the fractal dimensions of vegetation and soil in a managed larch(Larix gmelini) forest in Daxingan Mountains, NE China were estimated separately, and their scale correlation was discussed. (1) The dominant species of the larch forest, larch and the important accompany species, birch (Betula platyphylla) were homogeneously distributed along the transect. The other species were heterogeneous, and can be divided into three groups located on the right, left and central parts of the transect respectively. The transect can be separated into two parts at the position for the 29th quadrat(580m). (2) The fractal dimension in the large scale range from 200 to 400m was lower than that in the small scale range from 0 to 200m in the forest, indicating the different variation regularities of spatial heterogeneity in different scale ranges. The inflection point at 200m was one of the key scales of spatial hierarchy of the larch forest. (3) The scale variation of the forest was correlated with that of soil pH on large scales, which reflected the indirect control of the dominant species to the spatial pattern and species distribution of understorey by changing soil acidity in the forest and the indirect effects of forest management.

关键词： fractal dimension larch forest scale variation soil spatial heterogeneity species linkX

Combining sap flow measurement-based canopy stomatal conductance and 13C discrimination to estimate forest carbon assimilation

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Science Bulletin 2005年第18期 55-61页

作者： ZHAO Ping1, LU Ping2, MA Ling1, SUN Guchou1, RAO Xingquan1, CAI Xi’an1 & ZENG Xiaoping1 1. South China Institute of Botany, Chinese Academy of Sciences, Guangzhou 510650, China 2. csiro plant industry, Darwin laboratory, PMB44 Winnellie, NT 0822, australia

The available methods for studying C uptake of forest and their problems in practices are reviewed, and a new approach to combining sap flow and 13C techniques is proposed in this paper. This approach, obtained through strict mathematic derivation, combines sap flow measure-ment-based canopy stomatal conductance and 13C discrimi-nation to estimate instantaneous carbon assimilation rate of a forest. Namely the mean canopy stomatal conductance (gc) acquired from accurate measurement of sap flux density is integrated with the relationship between 13C discrimination (?) and Ci/Ca (intercellular/ambient CO2 concentrations) and with that between Anet (net photosynthetic rate) and gCO2 (stomatal conductance for CO2) so that a new relation be-tween forest C uptake and ? as well as gc is established. It is a new method of such kind for studying the C exchange be-tween forest and atmosphere based on experimental ecology.

关键词： sap flow stable carbon isotope C assimilation Granier’s thermal dissipation probe mean canopy stomatal conductance.

DNA chip-based expression profile analysis indicates involvement of the phosphatidylinositol signaling pathway in multiple plant responses to hormone and abiotic treatments

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Cell Research 2004年第1期14卷 34-45页

作者： Wen Hui LIN, Rui YE, Hui MA, Zhi Hong XU, Hong Wei XUE National laboratory of plant Molecular Genetics, Institute of plant Physiology and Ecology, Shanghai Institutes for Biological Sciences (SIBS), Chinese Academy of Sciences and Partner Group of Max-Planck-Institute of Molecular plant Physiology (MPI-MP) on "plant Molecular Physiology and Signal Transduction", 300 Fenglin Road, 200032 Shanghai, China Xue HW Chinese Acad Sci Shanghai Inst Biol Sci Inst Plant Physiol & Ecol Natl Lab Plant Mol Genet 300 Fenglin Rd Shanghai 200032 Peoples R China

The phosphatidylinositol (PI) metabolic pathway is considered critical in plant responses to many environmental factors, and previous studies have indicated the involvement of multiple PI-related gene families during cellular responses. Through a detailed analysis of the Arabidopsis thaliana genome, 82 polypeptides were identified as being involved in PI signaling. These could be grouped into different families including PI synthases (PIS), PI-phosphate kinases (PIPK), phospholipases (PL), inositol polyphosphate phosphatases (IPPase), inositol polyphosphate kinases (IPK), PI transfer proteins and putative inositol polyphosphate receptors. The presence of more than 10 isoforms of PIPK, PLC, PLD and IPPase suggested that these genes might be differentially expressed during plant cellular responses or growth and development. Accordingly, DNA chip technology was employed to study the expression patterns of various isoforms. In total, 79 mRNA clones were amplified and used for DNA chip generation. Expression

关键词： phosphatidylinositol signal transduction DNA chip expression profile.

Ammonium improves iron nutrition by decreasing leaf apoplastic pH of sunflower plants(Helianthus annuus ***)

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Chinese Science Bulletin 2003年第20期48卷 2215-2220页

作者： ZOU Chunqin & ZHANG Fusuo Key laboratory of plant-Soil Interactions, Ministry of Education Key laboratory of plant Nutrition, Ministry of Agriculture of China Department of plant Nutrition, China Agricultural University, Beijing 100094, China Correspondence should be addressed to Zhang Fusuo (e-mail: zhangfs@ ***) Key laboratory of plant-Soil Interaction Ministry of Education Key Laboratory of Plant Nutrition Ministry of Agriculture of China Department of Plant Nutrition China Agricultural University Beijing China

The effect of nitrogen form on pH and concentration of soluble iron (Fe) in leaf apoplast was investigated in hydrophonically grown sunflower plants (Helianthus annuus L. cv. Frankasol), and the mechanism underlying the improved Fe nutrition by ammonium (NH4) supply was also elucidated. Ammonium supply ameliorated Fe nutrition of plants grown without Fe through decreasing apoplastic pH and increasing soluble Fe concentration in apoplastic fluid of young leaves. The soluble Fe concentration in apoplastic fluid and cell sap of young leaves, and xylem exudates of NH4 fed-plants was higher than that of nitrate (NO3) fed-plants, and no typical Fe-deficiency chlorosis in young leaves was observed in NH4 fed plant without Fe supply. The apoplastic pH was 6.15 and 5.94 in young leaves of Fe-deficient plants fed respectively with NO3 and NH4, while in Fe-sufficient plants, the apoplastic pH was 6.43 with NO3, and 5.50 with NH4 supply. In primary leaves, the apoplastic pH was around 6.25 irrespective of nitrogen form and Fe supply. The pH of xylem exudate was 5.72 in Fe-deficient plants fed with NO3 and 5.49 with NH4. Iron nutrition increased the pH of xylem exudate by 0.27 and 0.16 unit under NO3 and NH4 supply respectively.

关键词：向日葵铁营养学非原质体 pH值氮铵赋存形态植物生理学

Measures against the adverse impact of natural wind on air-cooled condensers in power plant

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Science China(Technological Sciences) 2010年第5期53卷 1320-1327页

作者： YANG LiJun,DU XiaoZe & YANG YongPing Key laboratory of Condition Monitoring and Control for Power plant Equipment of Ministry of Education of China,North China Electric Power University,Beijing 102206,China 1. Key laboratory of Condition Monitoring and Control for Power plant Equipment of Ministry of Education of China North China Electric Power University Beijing 102206 China

The natural wind plays disadvantageous roles in the operation of air-cooled steam condensers in power *** is of use to take various measures against the adverse effect of wind for the performance improvement of air-cooled *** on representative 2×600 MW direct air-cooled power plant,three ways that can arrange and optimize the flow field of cooling air thus enhance the heat transfer of air-cooled condensers were *** physical and mathematical models of air-cooled condensers with various flow leading measures were presented and the flow and temperature fields of cooling air were obtained by CFD *** back pressures of turbine were calculated for different measures on the basis of the heat transfer model of air-cooled *** results show that the performance of air-cooled condensers is improved thus the back pressure of turbine is lowered to some extent by taking measures against the adverse impact of natural wind.

关键词： air-cooled condenser wind speed and direction flow and heat transfer back pressure power plant

Cloning and expression of putative ethylene receptor genes in soybean plant

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Progress in Natural Science:Materials International 2007年第10期17卷 1152-1160页

作者： Xie Zongming1,2,3, Lei Gang1,2, Wuriyanghan Hada1,2, Tian Aiguo1, Zhang Jinsong1 and Chen Shouyi1(1. National Key laboratory of plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China 2. Graduate School of Chinese Academy of Sciences, Beijing 100039, China 3. Xinjiang Academy of Agricultural and Reclamation Science, Shihezi 832000, China) National Key laboratory of plant Genomics Institute of Genetics and Developmental Biology Chinese Academy of Sciences Beijing 100101 China Graduate School of Chinese Academy of Sciences Beijing 100039 China Xinjiang Academy of Agricultural and Reclamation Science Shihezi 832000 China

Ethylene plays important roles in plant growth, development, and stress responses, and ethylene receptors have been identified and studied extensively in various plant species. Here we report the cloning of four ethylene receptor genes from soybean, i.e. GmETR1, GmERS1, GmETR2 and GmEIN4. Construction of the phylogenic tree showed that GmETR1 and GmERS1 belong to subfamily I whereas GmETR2 and GmEIN4 belong to subfamily II. The four ethylene receptor genes showed different tissue-specific expression patterns in roots, stems, leaves, cotyledons, flowers, pods and seeds of soybean. These genes were differentially regulated by various abiotic stresses and plant hormones. The possible roles of the four genes in soybean plant were also discussed.

关键词： abiotic stress expression analysis ethylene receptor plant hormone soybean.

Applications of stable isotopes to study plant-animal relationships in terrestrial ecosystems

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Chinese Science Bulletin 2004年第22期49卷 2339-2347页

作者： WANG Jianzhu1,LIN Guanghui1,2,HUANG Jianhui1 & HAN Xingguo1 1.Key laboratory of Quantitative Vegetation Sciences and Research Center for plant Ecology and Biodiversity Conservation,Institute of Botany,Chinese Academy of Sciences,Beijing 100093,China 2.Department of Global Ecology,Carnegie Institution of Washington,Stanford,CA 94305,USA Key laboratory of Quantitative Vegetation Sciences and Research Center for plant Ecology and Biodiversity Conservation Institute of Botany Chinese Academy of Sciences Beijing 100093 China Department of Global Ecology Carnegie Institution of Washington Stanford CA 94305 USA

As natural tracers, stable isotopes have been extensively used in plant physiological, ecological and envi- ronmental research. Recently, animal physiological ecologists have also applied stable isotope techniques to study plant- animal relationships. The isotopic compositions of animal body generally reflect and integrate their diets over a time period ranging from hours to years to the lifetime of an indi- vidual. When animal living habitat changes or animals move to a new environment, the animal isotopic compositions will shift accordingly. Thus, stable isotope signatures of an ani- mal can truly reflect its food sources, habitat, distribution and movement patterns during a given time period. More- over, by analyzing animal-tissue isotopic compositions at different temporal scales, we can improve our understanding of animal adaptation to environmental changes. Stable iso- tope technique also provides an ideal tool to study animal foodweb relationship and community structure because of isotopic fractionation during the processes of nutrient as- similation by animals. Stable isotope technique can continu- ously measure animal trophic position in a foodweb, which can eventually reveal the predator-prey relationship and its role in determining matter balance and energy flow in the entire ecosystem. Stable isotope technique has been one of the most important and efficient tools in studying plant-animal relationship. In this paper, we first review recent advances in the application of stable isotope techniques to plant-animal relationship research then evaluate their advantages and disadvantages, and finally discuss some future directions associated with stable isotope applications to plant-animal relationship research.

关键词：稳定同位素植物-动物关联性食物来源营养水平动物迁移