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Efflux of potassium ion is an important reason of HL-60 cells apoptosis induced by tachyplesin

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Acta Pharmacologica Sinica 2006年第10期27卷 1367-1374页

作者： Hal-tao ZHANG~(2.4) Jun WU~3 Hai-feng ZHANG~2 Qi-feng ZHU ~2Institute of Biochemistry.and Molecular Biology,Guangdong Medical College,Zhanjiang 524023,China ~3Affiliated Hospital of Guangdong Medical College,Zhanjiang 524023,China Institute of Biochemistry and Molecular Biology Guangdong Medical College Zhanjiang 524023 Chin

Aim:To investigate the role of intercellular potassium in tachyplesin-induced HL-60cells ***:The concentration of intercellular potassium,cell vol-ume and mitochondrial membrane potential were examined by flow ***:The concentration of intercellular potassium reduced in a time-depen-dent manner in tachyplesin-treated HL-60 *** addition,the loss of mitochon-drial membrane potential was tightly coupled with the shrinkage of cell *** caspase inhibitors protected against DNA degradation but did not pre-vent the loss of HL-60 cell viability induced by ***2+,which was akind of blocker of volume-regulatory K+channels,increased the viability oftachyplesin-treated HL-60 cells and maintained mitochondrial membrane potentialand cell ***:Efflux of K+was an important reason for apoptosisin tachyplesin-treated HL-60 *** of K+affected the viability of tachyplesin-treated HL-60 cells independent of the process of caspase activation.

关键词： horseshoe crab tachyplesin intracellular potassium mitochondrial membrane potential HL-60 cell

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粉喷短桩在某软基处理中的应用研究

土工基础 2002年第3期16卷 11-14页

作者：钟小林周全能王祥上海华东铁路建设监理有限公司蚌埠分公司安徽蚌埠233000 铁道第四勘察设计院湖北武汉430063

以萧甬铁路增建二线软土地基试验粉喷桩处理段的实测资料为例 ,对不同类型的粉喷桩处理软基的效果进行了分析 ,针对当地的工程地质条件 ,提出采用粉喷短桩处理软基具有技术可行、经济合理的优点 ,全线采用粉喷桩处理时宜采用短桩。

关键词：粉喷短桩软基处理萧甬铁路工程地质

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Cardioprotective effects of ghrelin and des-octanoyl ghrelin on myocardial injury induced by isoproterenol in rats

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Acta Pharmacologica Sinica 2006年第5期27卷 527-535页

作者： LLian LI~2 Li-ke ZHANG~5 Yong-zheng PANG~(2.4) Chun-shui PAN~(3.4) Yong-fen QI~(3.4) Li CHEN~4 Xian WANG~(3,4) Chao-shu TANG~(3,4) Jing ZHANG~(2.4,6) ~2Institute of Cardiovascular Research,Peking University First Hospital,Beijing 100034,China:~3Department of Physiology and Pathophysiology,Peking University Health Science Center,Beijing 100083,China ~4Key Laboratory of the Ministry of Education on Molecular Cardiology,Beijing 100083,China:~5Department of Pathophysiology,Capital University of Medicine,Beijing 100054,China lnstituteofCardiovascularResearch PekingUniversityFirstHospitalBeijing100034China DepartmentofPathophysiology CapitalUniversityofMedicineBeijing100054China

Aim:To determine the cardioprotective action of ghrelin and des-octanoyl ghrelinin rats with isoproterenol-induced myocardial ***:Rats were subcu-taneously injected with isoproterenol （ISO;20,10,and 5 mg/kg） on d 1,2 and 3,respectively,and then 3 mg/kg for the next 7d with or without ghrelin or des-octanoyl-ghrelin （100μg/kg,twice daily）.Plasma ghrelin and growth hormonelevels were assayed using radioimmunoassay *** hormone secreta-gogue receptor （GHSR） and ghrelin mRNA were determined using *** binding capacity and the affinity for [3H]ghrelin were determined by re-ceptor binding ***:Compared with controls,ISO-treated rats showedsevere myocardial injury,cardiomegaly,infarction-like necrosis and massive fi-brosis with increases in irradiated-ghrelin （ir-ghrelin） content in plasma by 67%and myocardia by 66% and in the mRNA level in the myocardia by 93% （P<0.01）.ISO-treated rats had 95% （P<0.01） higher GHSR mRNA levels in the *** maximal binding capacity of [3H]ghrelin for myocardial sarcolemma was higherin ISO-treated rats than in *** administration improved cardiac func-tion and ameliorated cardiomegaly and attenuated myocardial lipid peroxidationinjury and relieved cardiac fibrosis as compared with ISO treatment ***-istration of des-octanoyl ghrelin effectively antagonized ISO-induced myocardialinjury and improved all parameters ***,the therapeutic effect ofdes-octanoyl ghrelin was significantly weaker than that of *** plasmagrowth hormone level increased markedly,by 1.5-fold （P<0.01）,with ghrelinadministration as compared with that in controls,but was unaltered in the des-octanoyl ghrelin ***:Myocardial ghrelin and GHSR were up-regulated during ISO-induced myocardial *** protective effect of ghrelinagainst ISO-induced cardiac function injury and fibrosis was more potent thanthat of des-octanoyl ghrelin,which suggests that ghrelin could be an endog

关键词： ghrelin des-octanoyl ghrelin myocardium fibrosis isoproterenol

Ｆｒｉｅｎｄ病毒诱发小鼠红白血病生物学特性的进一步研究

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北京实验动物科学 1993年第4期10卷 11-14页

作者：褚建新华国勋丁立应红光李卫萍李景德中国医学科学院血液学研究所

本文报道了F-MuLV诱发小鼠红白血病生物学特性的进一步研究结果。发现中国昆明小鼠和615小鼠对F-MuLV有明显抵抗。脾脏的原红细胞是该病毒的靶细胞,但切脾后仍可发病,这时骨髓成为该病毒的靶器官。因而提出,在正常动物中F-MuLV对脾红系... 详细信息

本文报道了F-MuLV诱发小鼠红白血病生物学特性的进一步研究结果。发现中国昆明小鼠和615小鼠对F-MuLV有明显抵抗。脾脏的原红细胞是该病毒的靶细胞,但切脾后仍可发病,这时骨髓成为该病毒的靶器官。因而提出,在正常动物中F-MuLV对脾红系细胞比骨髓更具有亲和性。

关键词：小鼠红白血病敏感性弗氏病毒

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