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New Method of Cotton Flow Speed Detection Based on Area CCD

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New Method of Cotton Flow Speed Detection Based on Area CCD

Hydrogen peroxide-induced changes in intracellular pH of guard cells precede stomatal closure

亚洲精细农业与计算机农业应用联合大会

作者： Gaili Gao Daoliang Li Shouhai Shang College of Engineering china Agricultural university beijing 100083 china College of Information and Electrical Engineering china Agricultural university beijing 100083 chinaCollege of Engineering china Agricultural university beijing 100083 china

In this paper, it is first proposed that area CCD may be used to detect the movement speed of cotton or foreign fibers in a cotton flow pipeline. A new method is presented for using the RGB color coordinates to identify CCD images of the cotton flow and adopting the RGB coordinate distance to distinguish the exact location of the same cotton line from two adjacent images. Eight CCD images of the different foreign fiber in cotton, a CCD image of raw cotton and a CCD image with a black background are selected. A 6×6 pixel small image is chosen from each of that ones. The RGB color coordinates of the small images are calculated with the use of the function from the Matlab image processing tools. It may be found that the RGB coordinate data of the different types of foreign fibers, the cotton and black background have a marked difference. By tracking the RGB coordinate datum sequence of some line pixel points in the images, the exact location of movement cotton or foreign fibers in two adjacent images can be identified. And furthermore according to the CCD frame rate, the instantaneous speed of the cotton flow or foreign fibers can be got.

关键词： cotton foreign fiber speed detection.

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Cell Research 2001年第1期11卷 37-43页

作者： ZHANG XIAO, FA CAI DONG, JUN FENG GAO, CHUN PENG SONG (Department of Biology, Henan university, Kaifeng 475001, china) (College of Life Sciences, Northwest Sci-Tech university of agriculture and Forestry, Yangling 712100, china) 2College of Life Sciences Northwest Sci-Tech University of Agriculture and Forestry Yangling 712100 China 1Department of Biology Henan University Kaifeng 475001 China 2College of Life Sciences Northwest Sci-Tech University of Agriculture and Forestry Yangling 712100 China 1Department of Biology Henan University Kaifeng 475001 China

Epidermal bioassay demonstrated that benzylamine, a membrane-permeable weak base, can mimick hydrogen peroxide (H2.2. to induce stomatal closure, and butyric acid, a membrane-permeable weak acid, can partly abolish the H2.2.induced stomatal closure. Confocal pH mapping with the probe 5-(and-6)- carboxy seminaphthorhodafluor- 1 - acetoxymethylester (SNARF-1-AM) revealed that H2.2.leads to rapid changes in cytoplasmic and vacuolar pH in guard cells of Viola faba L, i. e. alkalinization of cytoplasmic areas occur red in parallel with a decrease of the vacuolar pH, and that butyric acid pretreatment can abolish alkalinization of cytoplasmic areas and acidification of vacuolar areas of guard cells challenged with H2.2. These results imply that the alkalinization of cytoplasm via efflux of cytosol protons into the vacuole in guard cells challenged with H2.2.is important at an early stage in the signal cascade leading to stomatal closure.

关键词： H2.2 pH SNARF-1-AM confocal Vicia guard cell.

The ∧∧ Bond Energy in Double-∧ Hypernuclei

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The ∧∧ Bond Energy in Double-∧ Hypernuclei

Effects of repeated applications of fungicide carbendazim on its persistence and microbial community in soil

第十三届全国核结构研讨会暨第九次全国“核结构与量子力学”专题讨论会

作者： H.F.L~(1 1)) 1(College of Science,china agriculture university,beijing 100083,P.R.china)

To provide guideline for near future experiments proposed at PANDA@FAIR and J-PARC,the∧∧bond energy in double-∧hypernuclei are explored with a new effective∧N interaction PK1-Y1,which is obtained by optimizing both hyperon binding energy and spin-orbit splitting within a relativistic mean field approach. The AA bond energy under case with onlyσ,ωmesons exchanged is found to be consistent with a perturbation calculation based on a Skyrme-Hartree-Fock *** the strange mesons such as scalarσ and vectorΦ,the net contribution fromσ andΦhas sharply reduced the∧∧bond *** addition,core polarization effect is shown tiny in heavier double-∧hypernuclei.

关键词： bond energy hypernuclei core polarization

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Journal of Environmental Sciences 2009年第2期21卷 179-185页

作者： YU Yunlong,CHU Xiaoqiang,PANG Guohui,XIANG Yueqin,FANG Hua Department of Plant Protection,College of agriculture and Biotechnology,Zhejiang university,Hangzhou 310029,china. Department of Plant Protection College of Agriculture and Biotechnology Zhejiang University Hangzhou 310029 China

Carbendazim, a systemic benzimidazole fungicide, is applied repeatedly to control plant diseases including soilborne diseases, over a growing season. Studies were carried out under laboratory conditions to assess the effects of repeated carbendazim applications on its persistence and microbial community in soil. The results indicate that dissipation of carbendazim in soil was accelerated with its application frequency. The degradation rate constant of carbendazim was increased significantly from 0.074 d-1 to 0.79 d-1. The corresponding half-life was shorten markedly from 9.3 d to 0.9 d after four repeated applications. No significant inhibitory effect of carbendazim on soil microbial utilization of the carbon sources was observed after first treatment, but a slight increase in average well color development （AWCD） was shown after second, third, and fourth applications. It suggested that soil microorganisms become adapted to carbendazim after repeated application. Simpson and Shannon indexes of soil microbial community from carbendazim treated soil were also similar to those from the control soil, indicating that the richness and dominant character of soil microorganisms remain unchangeable after repeated application. However, after first, second, and third addition of carbendazim, McIntosh indexes on day 21 were significantly higher compared with the control, suggesting that balance of soil microorganisms was altered due to the enrichment of the specific carbendazim-adapting strains in soil

关键词： carbendazim degradation soil microorganism repeated application

Manufacture and Properties of SiC(p)/AI Composites Made by Squeeze Casting

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Rare Metals 1993年第4期12卷 308-311页

作者：李义春尹丽娟安希墉 General Research Institute for Non-ferrous Metals Beijing 100088.China beijing agriculture Engineering university Beijing 100083China Harbin Institute of Technology Harbin 150006China

SiC(p)/Al composites(volume fraction of SiC V_f=30～40％)were manufactured successfully by squeeze casting,and its properties were *** reinforcements distributed homogeneously and bonded tightly with the matrix in the *** with the matrix alloy Grade 6061,the elastic modulus of SiC(p)/6061 composite is increased by 1.17 times,the tensile strength increased by 27％,while the coefficient of thermal expansion is decreased by 51％.

关键词： Squeeze casting Composite Manufacture

Effects of Panicle Nitrogen Fertilization on Non-Structural Carbohydrate and Grain Filling in Indica Rice

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Agricultural Sciences in china 2010年第11期9卷 1630-1640页

作者： ZHENG Yong-mei, DING Yan-feng, LIU Zheng-hui and WANG Shao-hua Key Laboratory of Crop Growth Regulation, Ministry of agriculture, Nanjing agriculture university, Nanjing 210 095, P.R.china [a] Key Laboratory of Crop Growth Regulation Ministry of Agriculture Nanjing Agriculture University Nanjing 210 095 P.R. China

Grain filling, a crucial stage of grain yield formation in rice, is usually affected by the panicle nitrogen （N） fertilization. Field and pot culture experiments were conducted to explore the underlying mechanisms of N effect. Two rice cultivars with high lodging resistance were grown in the field and pot. Four panicle N fertilization treatments were conducted in 2006 and repeated in 2007. The result showed that medium level of panicle N fertilization treatment （NM） enhanced the accumulation and translocation of non-structural carbohydrate （NSC） in the stem and sheath. Compared with non-nitrogen treatment （NO）, NM promoted the translocation of labeled ^13C from stem and sheath to grain. But, low level of panicle N fertilization treatment （NL） and high level of panicle N fertilization treatment （NH） showed the negative effect. The endosperm cell, grain length, and grain width of NM increased more quickly than that of NO from 4 to 10 d after anthesis. During the early period of grain filling, sucrose-phosphate synthase （EC 2.***.1.14, SPS） activity were significantly higher for the NM treatment than those of the NL and NH treatments. Sucrose synthase （EC 2.***.1.13, SuSase） activity in the grains was substantially enhanced by NM, with the duration of higher activity being longer than those of the other treatments. At maturing stage, NM significantly increased the filled grain number, the seed-setting rate, and the grain weight compared with NL and NH. The results suggest that NM have a positive effect on the activities of enzymes of physiological importance, thereby increasing the grain size and promoting grain filling.

关键词： rice panicle nitrogen fertilization grain filling non-structural carbohydrate sink capacity sucrose-phosphatesynthase sucrose synthase

Cloning and expression profiles of 15 genes encoding WRKY transcription factor in wheat (Triticum aestivem L.)

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Progress in Natural Science:Materials International 2008年第6期18卷 697-705页

作者： Hualing Wu,Zhongfu Ni,Yingyin Yao,Ganggang Guo,Qixin Sun* Department of Plant Genetics&Breeding and State Key Laboratory for Agrobiotechnology,Key Laboratory of Crop Heterosis and Utilization,Ministry of Education,Key Laboratory of Crop Genomics and Genetic Improvement,Ministry of agriculture/beijing Key Laboratory of Crop Genetic Improvement,china Agricultural university,beijing 100094,china Department of Plant Genetics & Breeding and State Key Laboratory for Agrobiotechnology Key Laboratory of Crop Heterosis and Utilization Ministry of Education Key Laboratory of Crop Genomics and Genetic Improvement Ministry of Agriculture/Beijing Key Laboratory of Crop Genetic Improvement China Agricultural University Beijing 100094 China

WRKY proteins are involved in various physiological processes,including biotic and abiotic stress responses,hormone responses and ***,no systematic identification,expression and function analysis of WRKY genes in wheat were *** this study,we isolated 15 wheat cDNAs with complete open reading frame(ORF)encoding putative WRKY proteins using in silico *** analysis indicated that the 15 wheat WRKY genes belonged to three major WRKY *** analysis revealed that most genes expressed drastically in leaf,except TaWRKY10 which expressed in crown *** genes were strongly up-reg-ulated with the senescence of *** genes were responsive to low temperature,high temperature,NaCl or PEG ***-over,dierential expression patterns were also observed between wheat hybrid and its parents,and some genes were more responsive to PEG treatment in the *** results demonstrated that wheat WRKY genes are involved in leaf senescing and abiotic *** the changed expression of these WRKY genes in hybrid might contribute to the heterosis by improving the stress tolerance in hybrids.

关键词： Wheat WRKY transcription factor Gene cloning Gene expression Heterosis

ON THE EXPLOITATION AND SUSTAINED UTILIZATION OF REGIONAL RESOURCES, TAKING THE DEVELOPMENT PROGRAM TO TAME THE HUANGHUAIHAI PLAIN AS AN EXAMPLE

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Bulletin of the Chinese Academy of Sciences 1995年第3期9卷 257-264页

作者： Shi Yuanchun(beijing university of agriculture)

The Huanghuaihai Plain is noted for its vast landmass, complex natural conditions and development problems to overcome. This article lists the achievements and experiences obtained by Chinese agrotechnicians during the past two decades in the comprehensive reclamation of the Plain, and based on these, it explores the potential of the Plain to make up the increasingly scarce local resources by S&T means.

关键词：刃口知心内曲

Cloning and characterization of a novel R1-MYB transcription factor in maize

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Progress in Natural Science:Materials International 2009年第9期19卷 1089-1096页

作者： Guanqing Jia, Bo Li, Dengfeng Zhang, Tifu Zhang, Zhiyong Li, Jingrui Dai, Shoucai Wang* National Maize Improvement Center of china, Key Laboratory of Crop Genomics and Genetic Improvement of agriculture Ministry, Department of Plant Genetics & Breeding, china Agricultural university, beijing 100193, china [a]National Maize Improvement Center of china Key Laboratory of Crop Genomics and Genetic Improvement of Agriculture Ministry Department of Plant Genetics & Breeding China Agricultural University Beijing 100193 China

R1-MYB transcription regulatory factors play vital roles in transcriptional modification during higher plant metabolism and development. In the present study, an R1-MYB gene was isolated from the maize (Zea mays L.) inbred line C8605-2 based on expressed sequence tags (ESTs), which expressed differentially between the hybrid C8605-2 × W1445 and its two parents in an oligonucleotide microarray. The full-length cDNA, designated as ZmMYBE1 (GeneBank Accession No. FJ024049), consists of 1992 nucleotides and contains a 1296-bp open reading frame. One conserved MYB domain was detected near the N-terminus of the deduced amino acid sequence, where an acidic Ser/Thr-rich area was also observed in the downstream region. The sequence of ZmMYBE1, mapped to chromosome 5 (bin 5.03), was revealed belonging to a multi-gene family in the maize genome. Subcellular localization analysis determined that ZmMYBE1 was located in the nucleus. Expression analysis showed that ZmMYBE1 transcripts accumulated in various tissues examined, with high expression levels in the immature ear and low levels in the tassel, root, and stem. The expression analysis for ZmMYBE1 in immature ears showed the highest expression level at the earliest vegetative developmental stage of the plant. Expression of ZmMYBE1 higher than that of the parents was observed in the hybrid C8605-2 × W1445. And also the expression level similar to the lowest of the parents in the hybrid C8605-2 × W245 was observed, revealing different expression patterns for the same gene in various hybrids combined from diversified inbred lines. Transgenic Arabidopsis overexpressing ZmMYBE1 implies that ZmMYBE1 has an important role in developmental regulation in maize.

关键词： MYB transcription factor Differential expression Maize