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Heter.sis of Double Low Self-incompatibility in oilseed r.pe(Br.ssica napus L.)

agricultural sciences in china 2002年第7期1卷 732-737页

作者： SHEN Jin-xiong, FU Ting-dong and YANG Guang-sheng( National key lab.r.tor. of Cr.p genetic improvement, National r.peseed genetic improvement Center.Huazhong agricultural Univer.ity, wuhan 430070 , p. r.. china oil crops research institute ,chinese academy of agricultural sciences , wuhan 430062., p. r.. china ) National key lab.r.tor. of Cr.p genetic improvement National Rapeseed Genetic Improvement CenterHuazhong Agricultural University Wuhan 430070 P. R . China oil crops research institute Chinese Academy of Agricultural Sciences Wuhan 430062 P. R .Chin National key lab.r.tor. of Cr.p genetic improvement National Rapeseed Genetic Improvement CenterHuazhong Agricultural University Wuhan 430070 P. R . China

66 F 1 hybr.ds, pr.duced by 3 double low self-incompatible lines and 22 var.eties with a Nor.h Car.lina II (NCII) cr.ssing design, wer. tested for.their.heter.sis in wuhan, china dur.ng two gr.wing seasons fr.m 1999 - 2001. The r.sults showed that significant differ.nces wer. found between F1s and their.par.nts for.yield per.plant and seed oil content. Mid-par.nt heter.sis of these two char.cter. r.nged fr.m 5.50% -64.11% and fr.m 1.55% -7.44% r.spectively. Heter.sis for.seed yield per.plant was gr.ater.than that of seed oil content. For.yield components, heter.sis of total number.of siliques per.plant was the highest, followed by seed number.per.silique and 1 000 seeds weight. Significant genotype-by-year.inter.ction was found for.seed yield per.plant. r.sults fr.m cor.elation and combining ability analysis indicated that par.ntal effects on its F! hybr.d depended on char.cter., seed yield per.plant was affected by both additive and non-additive effects, and seed oil content was affected mainly by additive effect. When designing hybr.d pr.gr.mme, par.nts might be selected by GCAs and var.ances of SCAs of par.nts for.the char.cter. affected by both additive and non-additive effects, and by the sum of GCAs of female and male par.nts for.the char.cter. mainly affected by additive effects.

关键词： Br.ssica napus L. Self-incompatibility Heter.sis Combining ability

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Inhibtion of Br.ssica napus Bn5 Gene Expr.ssion Enhances Susceptibility to the Vir.ses

Inhibtion of Brassica napus Bn5 Gene Expression Enhances Sus...

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中国植物病理学会2008年学术年会

作者： Cai Li~(1,2) Hou Mingsheng~1 Liu Shengyi~2 1. Depar.ment of plant Science and Technology, Huazhong agricultural Univer.ity, wuhan 430070,china 2..the key lab. of genetic improvement for oil crops, the ministry of agriculture, p r. china, oil crops research institute, chinese academy of agricultural sciences, wuhan 430062. china

A gene with putative function for.disease r.sistance was selected fr.m oilseed r.pe UniGene libr.r. and named as *** r.A inter.er.nce vector.of Bn5 gene was constr.cted and tr.nsfor.ed into *** L. by using the pollen ...

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Somatic mutation and development of DH lines r.sistant to Scler.tinia scler.tior.m in Br.ssica napus L

Somatic mutation and development of DH lines resistant to Sc...

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湖北省植物病理学会2002年学术年会

作者： S.Y. Liu J. ZHANG H. Z. WANG Z. Y. Xu W. YANG Y. Y. Liu key lab.r.tor. of genetic improvement for.oil crops, the ministry of agriculture, p.r. china, oil crops research institute, chinese academy of agricultural sciences, wuhan 430062. p.r.china

The stem r.t disease (Scler.tinia scler.tior.m) causes tr.mendous yield loss of oilseed r.pe (Br.ssica napus.) . This study was aimed to develop a str.tegy thr.ugh in vitr. mutation-selection for.r.si

关键词： Br.ssica napus Haploid somatic mutation r.sistance Scler.tinia scler.tior.m Matur.ty

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The r.ot development-r.lated(r.evr. Gene Incr.ases r.ot Gr.wth of Ar.bidopsis

The Root development-related(RDevR) Gene Increases Root Grow...

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湖北省遗传学会第八次代表大会暨学术讨论会

作者： Chenggang Wang,Guohua Chai,Lei Shi,Zetao Bai,Liming Xu,Caihua Dong,Junyan Huang,Shengyi Liu~* oil crops research institute,the key lab.for.oil Cr.p Biology,the ministry of agriculture,pr., chinese academy of agricultural sciences,wuhan,430062.china

The plasma membr.ne is a selectively per.eable lipid bilayer.found in all *** contains a wide var.ety of biological molecules,pr.mar.ly pr.teins and lipids,which ar. involved in a vast ar.ay of cellular.pr.cesses,such as cell adhesion,ion channel conductance and cell ***,we cloned a r.ot development-r.lated(r.evr. gene in Ar.bidopsis thaliana,which existed two mr.A splicing var.ants,named r.evr. and r.evr.,*** expr.ssion pr.ducts of r.evr. wer. pr.dicted to have five tr.nsmembr.ne domains,which belong to integr.l membr.ne family pr.teins. Subcellular.localization of r.evr.GFp fusion pr.tein in onion epider.al cells showed r.evr.was localized to the plasma ***-pCr.with the gene-specific pr.mer. showed r.evr. mr.A wer. abundant in r.ots,In contr.st,fewer.r.evr. mr.A wer. found in r.ot,but wer. abundant in *** seedling development,tr.nsgenic Ar.bidopsis thaliana which over.expr.ssed r.evr. exhibited longer.pr.mer.r.ots and mor. later.l *** to now,ther. wer. not any r.por.s about r.evr. biological functioa Our.r.sults pr.vided impor.ant insights into r.les of r.evr. pr.bably involved in the r.ot gr.wth.

关键词： r.evr.gene mr.A splicing var.ants membr.ne pr.tein r.ot gr.wth

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High-Thr.ughput discover. of chlor.plast and mitochondr.al DNA polymor.hisms in br.ssicaceae species by Or.-EcoTILLING

High-Throughput discovery of chloroplast and mitochondrial D...

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第十三届全国植物基因组学大会

作者： Changli Zeng~(1,2),Guangyong Wang~1,Jianbo Wang~2,Guixin Yan~1,Biyun Chen~1,Kun Xu~(1,) Jun Li~1,Guizhen Gao~1,Xiaoming Wu~(1*) 1 oil crops research institute of The chinese academy of agricultural sciences,key lab.r.tor. of Biology and genetic improvement of oil crops,ministry of agriculture,wuhan 430062.p.r.china 2 College of Life sciences,Jianghan Univer.ity,wuhan 430056,p.r.china

Infor.ation on polymor.hic DNA in or.anelle genomes is essential for.evolutionar. and ecological ***,it is challenging to per.or. high-thr.ughput investigations of chlor.plast and mitochondr.al DNA *** r.cent year., E...

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Quantitative Tr.it Loci for.Bor.n Use Efficiency in Br.ssica napus

Quantitative Trait Loci for Boron Use Efficiency in Brassica...

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第十二届全国植物基因组学大会

作者： Zunkang Zhao,Likun Wu,Lei Shi,Fangsen Xu~* National key lab.r.tor. of Cr.p genetic improvement,Micr.element research Centr.,Huazhong agricultural Univer.ity, wuhan 430070,china

Bor.n(B) is essential for.the gr.wn and development of higher.*** napus is one of the most sensitive crops to B *** studied,ther. ar. abundant genotype differ.nces in toler.nce to B deficiency among differ.nt Br.ssica napus ***,study on B nutr.tion genetic and molecular.biology in Br.ssica napus would be availab.e for.us to impr.ve oil r.peseed in B uptake and use. B-efficient Br.ssica napus cultivar.QY10 and B-inefficient cultivar.Bakow wer. scr.ened out at seedling and matur. stages,and then one BQ DH population was constr.cted by micr.spor. cultur. based on a cr.ss

关键词： Br.ssica napus Genotype Bor.n efficiency DH QTL

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Maize mutant selection for.ener.y cr.p discover.

Maize mutant selection for energy crop discovery

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The Second Inter.ational Symposium on Bioener.y and Biotechnology——in Conjunction with Miscanthus Wor.shop

作者： Bin Yu1,2,4,Wei Sun1,2,3,Fei Liu1,2,3,Lin Liu1,2,3,Jun Jia1,2,3,Wei Yang1,2,4,Xueqian Fu2,4,Yonglian Zheng2,4,Liangcai peng1,2,3,4 1 Biomass and Bioener.y research Centr.,Huazhong agricultural Univer.ity,wuhan 430070,china 2 National key lab.r.tor. of Cr.p genetic improvement,Huazhong agricultural Univer.ity,wuhan 430070,china 3 College of plant sciences and Technology,Huazhong agricultural Univer.ity,wuhan 430070,china 4 College of Life sciences and Technology,Huazhong agricultural Univer.ity,wuhan 430070,china

To r.duce the dependence on fossil oil for.tr.nspor.ation needs, biofuel is incr.asingly consider.d as a par.ial alter.ative. Maize is one of major.food crops over.the wor.d and

关键词： Maize mutant selection for.ener.y cr.p discover.

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Optimization of agr.bacter.um tumefaciens mediated soybean embr.onic tips genetic tr.nsfor.ation using or.hogonal exper.ment

Optimization of agrobacterium tumefaciens mediated soybean e...

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第六届中国植物逆境生理学与分子生物学学术研讨会

作者： Jie Zhang1,Lei Shang1,Wenyong Zheng1,Dongxu Zhang1,Mengchen Zhang2,Chun zhi Jiang2,Dongmei Wang2 1 College of Life Science,agricultural Univer.ity of Hebei,Baoding,071001,china 2 institute of Food and oil crops,Hebei academy of agricultural and For.str. sciences,Shijiazhuang 050031,china

＜正＞In or.er.to impr.ve the tr.nsfor.ation efficiency,Agr.bacter.um tumefaciens mediated tr.nsfor.ation system of soybean embr.onic tips was optimized by or.hogonal design using tr.nsient expr.ssion of GUS gene in this *** influency factor.（fer.enting liquor.of OD,infection time,inclusion of acetosyr.ngone and co-cultivation time r.stor. tr.in time）under.four.differ.nt levels wer. analysised by or.hogonal design.

关键词： soybean embr.onic tips or.hogonal design genetic tr.nsfor.ation Agr.bacter.um tumefacien

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Tr.nsgene silencing caused by 35S pr.moter.methylation in tr.nsgenic cotton plants

Transgene silencing caused by 35S promoter methylation in tr...

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江苏省遗传学会植物分子育种学术研讨会

作者： Wang Hai-Hai Wu Shenjie Guo Wangzhen Zhang Tianzhen (National key lab.r.tor. of Cr.p genetics & Ger.plasm Enhancement, Cotton research institute, Nanjing agricultural Univer.ity, Nanjing 210095, china)

Cotton genetic engineer.ng widely had been applied in pest management and improvement of fiber.quality and oil content of seeds. Tr.nsgene silencing had been widespr.ad r.vealed in many crops, but systemic study on gene silencing in tr.nsgenic cotton has not been r.por.ed. In this study, Embr.ogenic calli （EC） of cotton wer. tr.nsfor.ed by Agr.bacter.um str.in LBA4404 har.our.ng pBI121 binar. vector. After.cultur.d for.5-6 months, plantlets with 2-3 leaves wer. r.gener.ted and pCr.amplification was per.or.ed to detect npt-II and gus. In total, 97 tr.nsgenic plantlets har.or.ng both gus and npt-II wer. obtained. Fr.m these plantlets, 10 tr.nsgenic plantlets （10%） wer. r.vealed gus silencing tested by GUS assays and other.87 plantlets wer. GUS-positive. After.gr.fted these tr.nsgenic plantlets into gr.enhouse about 3 mouths, a GUS-negative plant （G-） and a GUS-positive plant （G+） gener.ted fr.m single calli line wer. chose to fur.her.study the silencing mechanism. Leaves of G- and G+ wer. fur.her.assayed by GUS staining and the r.sults showed GUS gene till expr.ssed stably in G+ plant and did not expr.ssed in G- plant, which cer.ainly suggested G- was a gus silencing tr.nsgenic cotton plant. r.-pCr.analysis indicated npt-II gene expr.ssed stably in leaves of G- and G+ plants;while GUS gene only expr.ssed in leave of G+ and no tr.nscr.ptem was detected in G- leaves, which was consistent with the GUS assays of G- and G+ tr.nsgenic cotton plants. It is r.mar.able that active of npt-II was not affected by gus silencing although they ar. adjacent to each another.in the tr.nsgenic locus separ.ted by about 700bp vector.DNA. Ther.for., silencing did not depend entir.ly on the position effect, although npt-II was used for.selection in this tr.nsfor.ation. Tr.nsgenic copy number.also affected gene expr.ssion and multi-copy inser.ion into genome could induce the DNA methylation. Souther. analysis showed these two tr.nsgenic plants had two copies, but we could not cer.ify whether

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Simultaneous identification and kar.otyping analysis of the chr.mosomes of *** by multiple BAC-FISH

Simultaneous identification and karyotyping analysis of the ...

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江苏省遗传学会第七届二次代表大会暨学术研讨会

作者： Kai Wang1, Bing Guan1, Wangzhen Guo, Baoliang Zhou, Yan Hu, Yichao Zhu, and Tianzhen Zhang2 (National key lab.r.tor. of Cr.p genetics and Ger.plasm Enhancement, Cotton research institute, Nanjing agricultural Univer.ity, Nanjing 210095, china)

＜正＞Cotton is one of the most impor.ant natur.l fiber.and edible oil crops in the wor.d. Significant pr.gr.ss has been made in the development of genetic maps for.the pur.ose of map-based gene

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