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Association of DRD2.polymorphisms and chlorpromazine-induced extrapyramidal syndrome in chinese schizophrenic patients

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Acta Pharmacologica Sinica 2006年第8期27卷 966-970页

作者： Sheng-nan WU~(2.3,5,6) Rui GAO~(2.3,6) Qing-he XING~(2.3,6) Hua-fang LI~4 Yi-feng SHEN~4 Niu-fan GU~4 Guo-yin FENG~4 Lin HE~(2.3,7)2.Bio-X Life Science research center,{2. Jiaotong University,{2. 2.0030,china 3 Institute of Nutritional sciences,SIBS,chinese academy of sciences,{2. 2.0031,china 4 {2. Institute of Mental Health,{2. 2.0030,china 5 {2. Institute of Hypertension,Ruijin Hospital,Medical School of {2. Jiaotong University,{2. 2.002.,china Bio-X Life Science research center Shanghai Jiaotong University Shanghai 200030 China

Aim:Extrapyramidal syndrome (EPS) is most commonly affected by typical antip-sychotic drugs that have a high affinity with the D2.***,manyresearch groups have reported on the positive relationship between the geneticvariations in the DRD2.gene and the therapeutic response in schizophrenia pa-tients as a result of the role of variations in the receptor in modulating *** this study,we evaluate the role DRD2.plays in chlorpromazine-induced EPS in schizophrenic ***:We identified seven SNP(singlenucleotide polymorphism) (-141Cins>del,TaqIB,TaqID,Ser311Cys,rs62.5,rs62.7and TaqIA) in the DRD2.gene in 146 schizophrenic inpatients (59 with EPS and 87without EPS according to the Simpson-Angus Scale) treated with chlorpromazineafter 8 *** alleles of all loci were determined by PCR (polymerase chainreaction).Results:Polymorphisms TaqlD,Ser311Cys and rs62.7 were not poly-morphic in the population recruited in the present *** statistical signifi-cance was found in the allele distribution of-141Cins>del,TaqIB,rs62.5 andTaqIA or in the estimated haplotypes (constituted by TaqIB,rs62.5 and TaqIA) inlinkage disequilibrium between the two ***:Our results did notlend strong support to the view that the genetic variation of the DRD2.gene playsa major role in the individually variable adverse effect induced by chlorpromazine,at least in chinese patients with *** results confirmed a previousstudy on the relationship between DRD2.and EPS in Caucasians.

关键词： dopamine receptors basal ganglia disease polymorphism chlorpromazine schizophrenia

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Anaerobic Induction of Isocitrate Lyase and Malate Synthase in Submerged Rice Seedlings Indicates the Important Metabolic Role of the Glyoxylate Cycle

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Acta Biochimica et Biophysica Sinica 2005年第6期37卷 406-414页

作者： Ying LU~(1.2. Yong-Rui WU~(1.2. Bin HAN~(1.3) 1 national center for {2. research. shanghai Institutes for Biological sciences. chinese academy of sciences. shanghai 2.02.3. china. 2.Graduate School of the chinese academy of sciences. Beijing 100039.china 3 Institute of Plant Physiology and Ecology. shanghai Institutes for Biological sciences. chinese academy of sciences. shanghai 2.0032. china nationalcenterfor{2.research ShanghaiInstitutesforBiologicalSciencesChineseAcademyofSciencesShanghai200233

The glyoxylate cycle is a modified form of the tricarboxylic acid cycle that converts C2.ompounds into C4 dicarboxylic acids at plant developmental *** studying submerged rice seedlings,we revealed the activation of the glyoxylate cycle by identifying the increased transcripts of mRNAs of thegenes of isocitrate lyase(ICL)and malate synthase(MS),two characteristic enzymes of the *** blot analysis showed that ICL and MS were activated in the prolonged anaerobic *** activity assay of pyruvate decarboxylase and ICL in the submerged seedlings indicated an 8.8-fold and3.5-fold increase over that in the unsubmerged seedlings,*** activity assay of acetyl-coen-zyme A synthetase in the submerged seedlings indicated a 3-fold increase over that in the unsubmergedseedlings,which is important for initiating acetate ***,we concluded that the glyoxylatecycle was involved in acetate metabolism under anaerobic conditions.

关键词： Oryza sativa glyoxylate cycle isocitrate lyase malate synthase acetyl-coenzyme A synthetase anoxia

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Construction of mini-Tn4001 transposon vector for Mycoplasma gallisepticum

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Science china(Life sciences) 2010年第11期53卷 1340-1345页

作者： CHEN HongJun, ZHAO ChunMei, SHEN XieYue, CHEN DanQing, YU ShengQing & DING Chan shanghai Veterinary research Institute, {2. academy of Agricultural sciences, shanghai 2.02.1, china 1. shanghai Veterinary research Institute Chinese Academy of Agricultural Sciences Shanghai 200241 China

The detailed genetic analysis of mycoplasmas has long been hampered by the lack of appropriate tools for genetic manipulation. In this study, the transposon vector, mini-Tn4001tetM, was constructed containing the tnp gene, encoding a transposase gene in Staphylococcus aureus, two copies of the IS2.6 inverted repeat sequence (inner and outer) and the tetM gene, from the Enterococcus faecalis Tn916 transposon, conferring resistance to tetracycline. This vector was electro-transformed into Mycoplasma gallisepticum (MG). The recombinant cells were screened by tetracycline selection. The results indicated that the transposon vector could replicate in MG strain R by successive passages, indicating that MG is a potential vector for expressing protective antigens of other pathogens.

关键词： Mycoplasma gallisepticum mini-Tn4001 transposon vector mycoplasma transformation

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Identification and Characterization of glnA Promoter and its Corresponding Trans-regulatory Protein GlnR in the Rifamycin SV Producing Actinomycete,Amycolatopsis mediterranei U32

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Acta Biochimica et Biophysica Sinica 2006年第12期38卷 831-843页

作者： Hao YU~(1&) Wen-Tao PENG~1 Yang LIU~1 Ting WU~2.Yu-Feng YAO~1 Ming-Xue CUI~1 Wei-Hong JIANG~1 Guo-Ping ZHAO~(1,3,4*) 1 Laboratory of Molecular Microbiology,Institute of Plant Physiology and Ecology,shanghai Institutes for Biological sciences,{2. academy of sciences,shanghai 2.0032.china 2.Department of Medicinal Chemistry,School of Pharmacy,Fudan University,shanghai 2.0032.china 3 State Key Laboratory of genetic Engineering & Department of Microbiology,School of Life Science,Fudan University,shanghai 2.0433,china 4 Laboratory of Disease and Health Genomics,{2. national Human Genome center at shanghai,shanghai 2.12.3,china Laboratory of Molecular Microbiology Institute of Plant Physiology and Ecology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai 200032 China Department of Medicinal Chemistry School of Pharmacy Fudan University Shanghai 200032 China State Key Laboratory of genetic Engineering & Department of Microbiology School of Life Science Fudan University Shanghai 200433 China Laboratory of Disease and Health Genomics Chinese National Human Genome Center at Shanghai Shanghai 201203 China

The genetic requirements for the transcription of glnA,encoding the major glutaminesynthetase in a rifamycin SV-producing Amycolatopsis mediterranei strain,U32.were *** experiments showed that the promoter of U32.glnA （pglnA） was likely to have two transcriptioninitiation sites:P1and P2.located 157 and 45 nucleotides （nt） upstream of the translational start codon,*** mobility shift and DNase I footprinting analyses revealed a 30 bp cis-element locatedat 45 to 75 nt downstream of P1,or 38 to 68 nt upstream of *** sequence of the cis-element displayedhigh similarity to the corresponding regions of pglnA from Streptomyces coelicolor and *** as a reporter gene,the expression levels of U32.pglnA and its deletion derivatives under differentnitrogen-source conditions were analyzed by detecting the catechol dioxygenase activities in ***54,*** J508 and *** FS10 （glnR mutant）.These in vivo studies showed that theactivation of U32.pglnA in *** required GlnR,and its binding to the U32.pglnA was furtherconfirmed by the gel mobility shift *** and heterologous expression of the U32.glnR allowed usto detect the in vitro interaction between the U32.GlnR and the corresponding pglnA *** shown by in vivo glnR inactivation and complementation indicated that GlnR is essential for theactive transcription of glnA in U32.

关键词： glnA promoter cis-element glnR Amycolatopsis mediterranei

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Finite element simulation of stretch forging using a mesh condensation method

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Science china(Technological sciences) 2010年第1期53卷 227-234页

作者： CHEN Wen & CUI ZhenShan national Die & Mold CAD Engineering research {2.,shanghai Jiao Tong University,shanghai 2.0030,china 1. national Die & Mold CAD Engineering research {2. Shanghai Jiao Tong University Shanghai 200030 China

In order to reduce the computation time of finite element simulations of stretch forging process,a mesh condensation method is presented and applied to a three-dimensional rigid-viscoplastic finite element *** this method,a conventional mesh for the whole zone of a workpiece is condensed to a computational mesh for the active deformation *** vital problems are solved,which are automatic construction of the computational mesh and treatment of interfaces between the deformation zone and the rigid *** mesh condensation method is compared with conventional finite element method by simulations of a six-bite stretch forging *** simulation results including forging load,temperature distribution and effective strain distribution are *** efficiency and accuracy of this method are verified.

关键词： mesh condensation method finite element method stretch forging incremental forging

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Are small RNAs a big help to plants?

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Science china(Life sciences) 2009年第3期52卷 212-223页

作者： MAO YingBo,XUE XueYi & CHEN XiaoYa national Key Laboratory of Plant Molecular genetics,Institute of Plant Physiology and Ecology,{2. Institutes for Biological sciences,chinese academy of sciences,{2. 2.0032.china 1. national Key Laboratory of Plant Molecular genetics Institute of Plant Physiology and Ecology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai 200032 China

The discovery of RNA interference(RNAi) has augmented our knowledge of gene regulation and presents a fascinating technology that has a great potential for application in genetic analysis,disease therapy,plant protection,and many other *** this review,we will focus on the biological functions of RNAi and its application in agriculture with a brief introduction to the history of its discovery and molecular mechanism.

关键词： RNA interference gene regulation plant protection

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Primary scientific results of Chang'E-1 lunar mission

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Science china Earth sciences 2010年第11期53卷 1565-1581页

作者： OUYANG ZiYuan1,2. LI ChunLai1, ZOU YongLiao1, ZHANG HongBo1, Lü Chang1, LIU JianZhong1, LIU JianJun1, ZUO Wei1, SU Yan1, WEN WeiBin1, BIAN Wei1, ZHAO BaoChang3, WANG JianYu4, YANG JianFeng3, CHANG Jin5, WANG HuanYu6, ZHANG XiaoHui7, WANG ShiJin7, WANG Min1, REN Xin1, MU LingLi1, KONG DeQing1, WANG XiaoQian1, WANG Fang1, GENG Liang1, ZHANG ZhouBin1, ZHENG Lei1, ZHU XinYing1, ZHENG YongChun1, LI JunDuo1, ZOU XiaoDuan1, XU Chun1, SHI ShuoBiao1, GAO YiFei1 & GAO GuanNan1 1 national Astronomical Observatories, chinese academy of sciences, Beijing 100012.{2. 2.Institute of Geochemistry, chinese academy of sciences, Guiyang 550002.{2. 3 Xi’an Institute of Optics and Precision Mechanics, chinese academy of sciences, Xi’an 710119,{2. 4 shanghai Institute of Technical Physics, chinese academy of sciences, shanghai 2.0083,{2. 5 Purple Mountain Observatory, chinese academy of sciences, Nanjing 2.0008,{2. 6 Institute of High Energy Physics, chinese academy of sciences, Beijing 100049,{2. 7 center for Space Science and Applied research, chinese academy of sciences, Beijing 100190,{2. 1. national Astronomical Observatories Chinese Academy of Sciences Beijing 100012 China2. Institute of Geochemistry Chinese Academy of Sciences Guiyang 550002 China3. Xi’an Institute of Optics and Precision Mechanics Chinese Academy of Sciences Xi’an 710119 China4. Shanghai Institute of Technical Physics Chinese Academy of Sciences Shanghai 200083 China5. Purple Mountain Observatory Chinese Academy of Sciences Nanjing 210008 China6. Institute of High Energy Physics Chinese Academy of Sciences Beijing 100049 China7. Center for Space Science and Applied Research Chinese Academy of Sciences Beijing 100190 China

The strategic plan for the development of the unmanned chinese Lunar Exploration Program is characterized by three distinct stages: "orbiting around", "landing on" and "returning from" the Moon. The first chinese lunar probe, Chang'E-1, which was successfully launched on October 2.th, 2.07 at Xichang Satellite Launch {2., and guided to crash on the Moon on March 1st, 2.09, at 52.36°E, 1.50°S, in the north of Mare Fecunditatis, is the first step towards the "orbiting around" stage. The Chang'E-1 mission lasted 495 days, exceeding the expected life-span by about four months. A total of 1.37 TB raw data was received from Chang'E-1. It was then processed into 4 TB scientific data products at various levels. Many scientific results have been obtained by analyzing these data, including especially the "global lunar image from the first chinese lunar explora- tion mission". All scientific goals of Chang'E-1 have been achieved. It provides much useful materials for further advances of lunar sciences and planetary chemistry. Meanwhile, these results will serve as a firm basis for future chinese lunar missions.

关键词： lunar orbiter Chang’E-1 scientific data results

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Construction of WCB-11:A novel phiYFP arsenic-resistant whole-cell biosensor

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Journal of Environmental sciences 2010年第9期22卷 1469-1474页

作者： Qing Hu ,Li Li,Yanjuan Wang,Wenjuan Zhao,Hongyan Qi,Guoqiang Zhuang Department of Environmental Bio-Technology,research center for Eco-Environmental {2.,chinese academy of {2.,Beijing 100085,china. [a] Department of Environmental Bio-Technology Research Center for Eco-Environmental Sciences Chinese Academy of Sciences Beijing 100085 China

The prediction and assessment of environmental pollution by arsenic are important preconditions of advocating environmental protection and human health risk assessment. A yellow fluorescent protein-based whole-cell biosensor for the detection of arsenite and arsenate was constructed and tested. An arsenic-resistant promoter and the regulatory gene arsR were obtained by PCR from the genome ofEscherichia coli DH5ct, andphiYFP was introduced into E. coli DH5ct as a reporter gene to construct an arsenic-resistant whole-cell biosensor （WCB-11） in which phiYFP was expressed well for the first time. Experimental results demonstrated that the biosensor has a good response to arsenic and the expression ofphiYFP. When strain WCB-11 was exposed to As^3＋ and As^5＋, the expression of yellow fluorescence was time-dependent and dose-dependent. This engineered construct is expected to become established as an inexpensive and convenient method for the detection of arsenic in the field.

关键词： arsenic reporter gene whole-cell biosensor yellow fluorescent protein (phiYFP)

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Intermittent hypoxia attenuates ischemia/reperfusion induced apoptosis in cardiac myocytes via regulating Bcl-2.Bax expression

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Cell research 2003年第5期13卷 385-391页

作者： JIAN WEN DONG, HAI FENG ZHU, WEI ZHONG ZHU, HAI LEI DING, TIE MIN MA, ZHAO NIAN ZHOU 1 Laboratory of Hypoxic Cardiovascular Physiology, shanghai Institutes for Biological sciences,{2., shanghai 2.0031, china.2.Department of Physiology and Pathophysiology, Health Science center Peking University, Beijing 100083 china 1Laboratory of Hypoxic Cardiovascular Physiology Shanghai Institutes for Biological SciencesChinese Academy of Sciences Shanghai 200031 China. E-mail: znzhou@*** 1Laboratory of Hypoxic Cardiovascular Physiology Shanghai Institutes for Biological SciencesChinese Academy of Sciences Shanghai 200031 China. E-mail: znzhou@*** 1Laboratory of Hypoxic Cardiovascular Physiology Shanghai Institutes for Biological SciencesChinese Academy of Sciences Shanghai 200031 China. E-mail: znzhou@*** 1Laboratory of Hypoxic Cardiovascular Physiology Shanghai Institutes for Biological SciencesChinese Academy of Sciences Shanghai 200031 China. E-mail: znzhou@*** 1Laboratory of Hypoxic Cardiovascular Physiology Shanghai Institutes for Biological SciencesChinese Academy of Sciences Shanghai 200031 China. E-mail: znzhou@*** 1Laboratory of Hypoxic Cardiovascular Physiology Shanghai Institutes for Biological SciencesChinese Academy of Sciences Shanghai 200031 China. E-mail: znzhou@***

Intermittent hypoxia has been shown to provide myocardial protection against ishemia/reperfusion-induced injury. Cardiac myocyte loss through apoptosis has been reported in ischemia/reperfusion injury. Our aim was to investigate whether intermittent hypoxia could attenuate ischemia/reperfusion-induced apoptosis in cardiac myocytes and its potential mechanisms. Adult male Sprague-Dawley rats were exposed to hypoxia simulated 5000 m in a hypobaric chamber for 6 h/day, lasting 42.days. Normoxia group rats were kept under normoxic conditions. Isolated perfused hearts from both groups were subjected to 30 min of global ischemia followed by 60 min reperfusion. Incidence of apoptosis in cardiac myocytes was determined by terminal deoxynucleotidyl transferase mediated dUTP nick end labeling （TUNEL） and DNA agarose gel electrophoresis. Expressions of apoptosis related proteins, Bax and Bcl-2. in cytosolic and membrane fraction were detected by Western Blotting. After ischemia/reperfusion, enhanced recovery of ca

关键词： intermittent hypoxia apoptosis cardiac myocytes Bax Bcl-2.

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The non-destructive analysis of ancient jade artifacts unearthed from the Liangzhu sites at Yuhang,Zhejiang

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Science china(Technological sciences) 2010年第12期53卷 3404-3419页

作者： GAN FuXi1,3, CAO JingYan2. CHENG HuanSheng3, Gu DongHong1, RUI GuoYao2. FANG XiangMing2. DONG JunQing1 & ZHAO HongXia1 1Scientific Archeology center, shanghai Institute of Optics and Fine Mechanics,{2., shanghai 2.1800, china 2.research Institute of Cultural Relics and Archeology of Zhejiang, Hangzhou 310014, china 3 Fudan University, shanghai 2.0433, china 1. Scientific Archeology center Shanghai Institute of Optics and Fine Mechanics Chinese Academy of Sciences Shanghai 201800 China3. Fudan University Shanghai 200433 China2. Research Institute of Cultural Relics and Archeology of Zhejiang Hangzhou 310014 China

Samples of the jade artifacts unearthed from the Liangzhu sites at Yuhang (Fanshan,Yaoshan, Huiguanshan and Tangshan) of Zhejiang province were described. The chemical composition, phase and jade structure of a hundred and more intact samples were analyzed by the non-destructive techniques of PIXE, XRD and laser Raman spectroscopy. Results showed that the minerals of the jade artifacts mostly belonged to tremolite and tremolite-actinolite. Relationship of the civilization development in the Neolithic age and the application of tremolite type mineral in jade artifacts was discussed. The contents of rare and trace elements in tremolite jade artfacts and in mineral samples were measured for the first time. The possible mineral sources of these tremolite type jade artifacts were discussed.

关键词： Liangzhu culture non-destructive analysis ancient jade artifacts essence and source

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