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Molecular cloning of growth hormone receptor (GHR) from common carp(Cyprinus carpio L.)and identification of its two forms of mRNA transcripts

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Progress in Natural Science:Materials International 2006年第11期16卷 1156-1163页

作者： SUN Xiaofeng~ 1,2., GUO Qionglin~1**, HU Wei~1, WANG Yaping~1 and ZHU Zuoyan~1(1. State Key Laboratory of Freshwater Ecology and Biotechnology, institute of Hydrobiology, chinese {2. of Sciences, Wuhan 430072. china 2. Graduate School of chinese {2. of Sciences, beijing 100039, china) State Key Laboratory of Freshwater Ecology and Biotechnology Institute of Hydrobiology Chinese Academy of Sciences Wuhan 430072 China Graduate School of chinese academy of Sciences Beijing 100039 China

The cDNA of growth hormone receptor (GHR) was cloned from the liver of 2.year common carp (Cyprinus carpio L.) by reverse transcription-polymerase chain reaction (RT-PCR) and rapid amplification of cDNA end (RACE). Its open reading frame (ORF) of 1806 nucleotides is translated into a putative peptide of 602.amino acids, including an extracellular ligand-binding domain of 2.4 amino acids (aa), a single transmembrane domain of 2. aa and an intracellular signal-transduction domain of 334 aa. Sequence analysis indicated that common carp GHR is highly homologous to goldfish (Carassius auratus) GHR at both gene and protein levels. Using a pair of gene-specific primers, a GHR fragment was amplified from the cDNA of 2.year common carp, a 2.4 bp product was identified in liver and a 32. bp product in other tissues. The sequencing of the products and the partial genomic DNA indicated that the difference in product size was the result of a 97 bp intron that alternatively spliced. In addition, the 32. bp fragment could be amplified from all the tissues of 4-month common carp including liver, demonstrating the occurrence of the alternative splicing of this intron during the development of common carp. Moreover, a semi-quantitative RT-PCR was performed to analyze the expression level of GHR in tissues of 2.year common carp and 4-month common carp. The result revealed that in the tissues of gill, thymus and brain, the expression level of GHR in 2.year common carp was significantly lower than that of 4-month common carp.

关键词： growth hormone receptor (GHR) molecular cloning transcript expression analysis common carp.

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A new principle of synthetic cascade utilization of chemical energy and physical energy

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Science china(Technological Sciences) 2005年第2期48卷 163-179页

作者： JIN Hongguang1, HONG Hui1,2. WANG Baoqun1,2. HAN Wei1,2.& LIN Rumou1,2.1. institute of Engineering Thermophysics, chinese {2. of Sciences, beijing 100080, china 2. Graduate School of the chinese {2. of Sciences, beijing 100039, china institute of Engineering Thermophysics Chinese Academy of Sciences Beijing China Graduate School of the chinese academy of Sciences Beijing China

We propose a new principle of the cascade utilization of both chemical energy and physical energy in energy systems with the integration of chemical processes and thermal cycles. Particularly, a general equation of energy levels of substance, Gibbs free energy of chemical reaction and physical energy is explicitly founded. On the basis of this equation, a chemical-looping combustion and an indirect combustion are investigated. Furthermore, a mechanism of energy release, with the combination of decreasing the energy level of Gibbs free energy and upgrading the energy level of low or middle- temperature thermal energy, is clarified. The promising results obtained here establish a theoretical basis for the further investigation of multi-function systems in which energy and the environment are compatible, and create a new approach to improve the performance of traditional thermal cycles.

关键词： chemical energy, cascade utilization, exergy, energy conversion.

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Simulating the impacts of climate variation and land-cover changes on basin hydrology: A case study of the Suomo basin

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Science china Earth Sciences 2005年第9期48卷 1501-1509页

作者： CHEN Junfeng1,2. LI Xiubin2.& ZHANG Ming2.1. institute of Remote Sensing & GIS, Peking University, beijing 100871,{2. 2. institute of Geographical Sciences and Natural Resources Research, chinese academy of Sciences, beijing 100101,{2. institute of Remote Sensing & GIS Peking University Beijing China institute of Geographical Sciences and Natural Resources Research Chinese Academy of Sciences Beijing Chin

Impacts of land cover changes on watershed hydrology have been a long-term academic concern with acute dispute. But little attention has been paid to such effects on mesoscale river basins, where the society has a closer link to river hydrology. The present study focuses on a mesoscale river basin, the Suomo Basin that is located on the upper reaches of the Yangtze River. Land covers in the basin in the years 1970, 1986 and 1999 were mapped. A lumped hydrologic model, CHARM, and a distributed hydrologic model, SWAT, were used to model the impacts of both land-cover change and climate variation on river runoff during the past four decades. The results show that the contribution of climate variation to the change of runoff regime makes up 60%―80%, while that of land cover changes only 2.%.

关键词： climate variation, land-cover changes, Suomo Basin, CHARM model, SWAT model.

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Crustal structure of Gondwana-and Yangtze-typed blocks:An example by wide-angle seismic profile from Menglian to Malong in western Yunnan

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Science china Earth Sciences 2005年第11期48卷 1828-1836页

作者： ZHANG Zhongjie1, BAI Zhiming1, WANG Chunyong2. TENG Jiwen1, Lü Qingtian3, LI Jiliang1, SUN Shanxue1 & WANG Xinzheng1 1. institute of geology and Geophysics, {2. academy of Sciences, beijing 10002., china 2. institute of Geophysics, {2. Seismological Bureau, beijing 100081, china 3. institute of Deposition Resource, {2. academy of Geological Sciences, beijing 100037, china institute of geology and Geophysics Chinese Academy of Sciences Beijing China institute of Geophysics Chinese Seismological Bureau Beijing China institute of Deposition Resource Chinese Academy of Geological Sciences Beijing China

The wide-angle seismic profile between Menglian and Malong crosses the Baoshan block (Gondwana-typed), and Simao and southwestern Yangtze blocks (Yangtze-typed). By in-terpreting the wide-angle seismic data, we obtained the seismic crust/upper mantle structure of P-wave velocities together with the seismic reflections of these three blocks, Changning- Menglian and Mojiang suture zones among the mentioned three blocks. Our interpreting results demonstrate that the P-wave crustal velocity of Simao block is slower than that of Baoshan and southwestern Yangtze block and the crustal thickness gradually thickens from the Baoshan block, Simao to southwestern Yangtze block. Crustal reflection patterns of these three blocks have dis-tinct differences too. For the Gondwana-typed blocks, seismic reflections in the upper crust are well developed while in middle-lower crust they are very weak. The crustal reflections in the Yangtze block are very well developed. The crustal reflection patterns in Simao and southwest-ern Yangtze blocks are distinguishable. The average thickness of the crust in the studied area is about 40 km. And we make some discussions on the crustal thickening model of the three blocks in western Yunnan and tectonic setting of seismic developing and interaction of Gondwana and Yangtze blocks.

关键词： Gondwana blocks,Yangtze blocks,wide-angle profile,crustal structure,tectonic setting

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Vermiform trace fossils from the Precambrian Ruyang Group, western Henan

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chinese Science Bulletin 1997年第3期42卷 251-254页

作者： HU Jianmin, MENG Qingren and LI Wenhou1 Xi’ an College of geology, Xi’an 710054, china 2. {2. of Geophysics, chinese academy of Sciences, beijing 100101, china 3 Department of geology, Northwest University, Xi’an 710069, china Xi’an College of geology Xi’an China institute of Geophysics Chinese Academy of Sciences Beijing China Department of geology Northwest University Xi’an China

THE origin and evolution of Precambrian lives has been a long-standing controversial problem. It is now generally accepted by most paleontologists that the origin of metazoa can be traced back to more than 1000 Ma, because the Ediacara and latest pre-Ediacara fauna, such as the Huainan fauna, are featured by their diversification of forms, complicated structures

关键词： Precambrian trace fossil Ruyang Group.

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Wet-chemical synthesis and characteristics of Au nanoshell

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Science china Chemistry 2005年第5期48卷 431-435页

作者： LIU Zhongxin1,2. SONG Hongwei1, YU Lixin1,2. YANG Linmei1,2.& PAN Guohui1,2.1. Key Laboratory of Excited State Physics, Changchun institute of Optics, Fine Mechanics and Physics, chinese {2. of Sciences, Changchun 130033, china 2. Graduate School of the chinese {2. of Sciences, beijing 100039, china Key Laboratory of Excited State Physics Changchun Institute of Optics Fine Mechanics and Physics Chinese Academy of Sciences Changchun China Graduate School of the chinese academy of Sciences Beijing China

Gold nanoshells were prepared by an easy wet-chemical method, with Ag nanoparticles used as the templates. Transmission electron microscopy (TEM) indicated that the shells were sphere in shape, the size was homogenous, being about 2. nm, and no hard ag-glomerates were observed. The plasma resonance absorption of gold was tuned from visible to near-infrared (NIR) with the increased volume of HAuCl4. The temperature grads (?T) of the gold nanoshell hydrosol under the exposure of an 808-nm optical fiber laser with different power den-sities were measured. The highest ?T was 30℃ (5 W/cm2. irradiation area was 2.cm2.. This kind of gold nanoshell hydrosol is a promising material to be used in biomedicine such as photo-thermal cancer therapy, and its special photothermal convert property will photothermally trigger drug release.

关键词： wet-chemical synthesis, Au, nanoshell, NIR absorption, photothermal conversion.

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Human remains and the environment of Early Pleistocene in the Nihewan Basin

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Science china Earth Sciences 2004年第5期47卷 437-444页

作者： CAI Baoquan1 & LI Qiang2.1. Department of History, Xiamen University, Fujian 361005, china 2. institute of Vertebrate Palaeontology and Palaeoanthropology, {2. academy of Sciences, beijing 100044, china Department of History Xiamen University Fujian 361005 China Inst. of Vertebrate Palaeontology Chinese Academy of Sciences Beijing 100044 China

A new Early Pleistocene Paleolithic site was found in July 2.01 in the Nihewan Ba-sin, Hebei Province. Totally 500 mammal specimens assigned to 2. species and 5 lithic artifacts were collected. The coexistence of Allophaiomys deucalion, Borsodia chinensis and Yangia tingi provides important evidence of chronology. On the basis of the comparison of mammalian fauna, the date of Paleolithic artifacts is probably earlier than 1.8 MaBP, possibly 2.0 MaBP. This is the earliest evidence of hominid activity found so far in North china. The hominid at that time in the Nihewan Basin lived in an environment of arid grasslands with scattered trees of temperate zone. This discovery is significant to the study of human origin and cultural development.

关键词： human remains, environment, Early Pleistocene, Nihewan.

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Effect of concentration on surfactant micelle shapes——A molecular dynamics study

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Science china Chemistry 2005年第5期48卷 470-475页

作者： GAO Jian1,2. GE Wei1 & LI Jinghai1 1. Multiphase Reaction Laboratory, institute of Process Engineering, chinese {2. of Sciences, beijing 100080, china 2. Graduate School of The chinese {2. of Sciences, beijing 100085, china Multiphase Reaction Laboratory Institute of Process Engineering Chinese Academy of Sciences Beijing China Graduate School of The chinese academy of Sciences Beijing China

Many aspects of the behavior of surfactants have not been well understood due to the coupling of many different mechanisms. Computer simulation is, therefore, attractive in the sense that it can explore the effect of different mechanisms separately. In this paper, the shapes, structures and sizes of sodium dodecylbenzenesulfonate (SDBS) micelles under different con-centrations in an oil/water mixture were studied via molecular dynamics (MD) simulations using a simplified atomistic model which basically maintains the hydrophile and lipophile properties of the surfactant molecules. Above the critical micellar concentration (cmc), surfactant molecules ag-gregate spontaneously to form a wide variety of assemblies, from spherical to rodlike, wormlike and bilayer micelles. Changes in their ratios of the principle moments of inertia (g1/g3, g2.g3) in-dicated the transition of micelle shapes at different concentrations. The aggregation number of micelle is found to have a power-law dependence on surfactant concentration.

关键词： surfactant, MD simulation, sodium dodecylbenzenesulfonate, micelle shape.

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Properties and applications of cold supersonic gas jet

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Science china(Physics,Mechanics & Astronomy) 2006年第6期49卷 709-715页

作者： ZHANG Shaofeng1,2.MA Xinwen1,2.LIU Huiping2.LI Bin1,2.& ZHU Xiaolong1,2.1. institute of Modern Physics,chinese {2. of Sciences,Lanzhou 730000,china 2. Graduate University of chinese {2. of Sciences,beijing 100049,china institute of Modern Physics Chinese Academy of Sciences Lanzhou China Graduate University of chinese academy of Sciences Beijing China

By analyzing the formation mechanism of a supersonic gas jet,a set of equations which describe the atomic beam properties were established. The influence of initial temperature,initial pressure,background gas pressure and pumping speed was discussed in detail. A simulation program was developed based on the equations,and the results under different initial conditions were obtained. The results are in good agreement with the experimental data,and suggest that,in order to get much smaller transverse momentum in collision experiments,it is necessary to lower the initial temperature and the initial pressure of the supersonic gas jet,together with increasing the pumping speed. These results are very instructive for construction of a new generation of cold supersonic gas jets.

关键词： cold supersonic gas jet,zone of silence,nozzle,skimmer.

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Case studies on mesoscale structures of heavy rainfall system in the Yangtze River generated by Meiyu front

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Science china Earth Sciences 2005年第8期48卷 1303-1311页

作者： LIU Liping1, RUAN Zheng1 & QIN Danyu2.1. chinese academy of Meteorological Sciences, {2. 100081, china 2. Nanjing institute of Meteorology, Nanjing 2.0044, china chinese academy of Meteorological Sciences Beijing China Nanjing institute of Meteorology Nanjing China

A heavy rainfall system was observed over the Yangtze River during Meiyu season by using dual-Doppler radar systems in the field experiment conducted by the project of National Fundamental Research Planning “Research on formation mechanism and the prediction theory of hazardous weather over china”. The three-dimensional mesoscale kinematic structure and process of a heavy rain on 2. and 2. July, 2.02.are investigated by using the radial velocity and dual-Doppler radar technique. The results show that a southwest-northeast oriented rain band with the length of about 1000 km involves numbers of meso-β or meso-γ-scale convective cells with the size of 2.―50 km. The heavy rain band in the middle reaches of the Yangtze River ex-ists in a low-level wind shear. The interaction between southwest low-level jet on the southern side of wind shear and east wind on the northern side formed the updraft. The wind disturbance, wind shear and convergence generate the convective action. The new cell developed in right rear flank of rain band and moved to the southwest wind area, where the vapor is abundant. This kind of echo can last a long time and developed well. The strong convective echoes are often ac-companied by the meso-γ-scale vortex and convergence.

关键词： heavy rainfall in Meiyu front, mesoscale structure, dual-Doppler radar, middle reaches of the Yangtze River, low-level jet.

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