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Overexpression of p2.^(KIP1)induced cell cycle arrest in G_1 phase and subsequent apoptosis in HCC-92.4 cell line

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World Journal of Gastroenterology 2000年第4期6卷 513-521页

作者： Jiang Li Xin Ke Yang Xin Xin Yu Meng Liang Ge Wen Liang Wang Jie Zhang Yun De Hou Department of Pathology,Fourth Military Medical University,Xi’an 710033,Shaanxi Province,china State Key Laboratory for Molecular Virology and Genetic Engineering,beijing 100052.china Department of Dermatology,beijing Hospital,beijing 100016,china institute of radiation medicine,beijing 100085,china 第四军医大学陕西西安中国预防医学科学院病毒所北京中国预防医学科学院病毒所分子病毒基因工程国家重点实验室北京北京医院北京解放军军事医学科学院放射医学所北京

AIM We have previously reported that inducible over-expresaion of Bak may prolong cell cycle in G1 phase and lead to apoptosis in HCC-92.4 cells. This study is to investigate whether p2.KIP1 plays an important role in this process. MEHODS In order to elucidate the exact function of p2.KIP1 in this process, a zinc inducible p2.KIP1 stable transfectant and transient p2.KIP1- GFP fusion transfectant were constructed. The effects of inducible p2.KIP1 on cell growth, cell cycle arrest and apoptosis were examined in the mock, control pMD vector, and pMD-KIP1 transfected HCC-92.4 cells. RESULTS This p2.KIP1-GFP transfectant may transiently express the fusion gene. The cell growth was reduced by 35% at 48 h of p2.KIP1 induction with zinc treatment as determined by trypan blue exclusion assay. These differences remained the same after 72.h of p2.KIP1 expression, p2.KIP1 caused cell cycle arrest after 2. h of induction, with 40% increase in G1 population. Prolonged p2.KIP1 expression in this cell line induced apoptotic cell death reflected by TUNEL assay. Fourty-eight h and 72.h of p2.KIP1 expression showed a characteristic DNA ladder on agarose gel electrophoresis.

关键词： p2.^(KIP1) apoptosis cell cycle inducible expression system carcinoma hepatocellular liver neoplasms

Inhibition of tumor growth and metastasis with antisense oligonucleotides (Cantide) targeting hTERT in an in situ human hepatocellular carcinoma model

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Acta Pharmacologica Sinica 2005年第6期26卷 762-768页

作者： Ru-xian LIN~2. Chao-wei TUO~3, Qiu-jun LU~2. Wei ZHANG~2. Sheng-qi WANG~(2.4) ~2.beijing institute of {2. medicine, beijing 100850, china, ~3 Department of Pathology, PLA 2.2.Hospital, Shenyang 110001, china beijinginstituteof{2.medicine Beijing100850China

Aim: To evaluate the in vivo antitumor effects of Cantide and the combined effect with 5-fluorouracil. Methods: An in situ human hepatocellular carcinoma model was established in mice livers orthotopically. Drugs were administered intravenously and tumor sizes were monitored with calipers. Plasma alpha-fetoprotein (AFP) were detected by radiation immunoassay. Morphology of tumors was evaluated by hematoxylin-eosin (H&E) staining of histological sections. Human telomerase reverse transcriptase (hTERT) protein levels were detected by Western blotting. Results: Cantide significantly inhibit in situ human hepatocellular carcinoma growth in mice with a 75 and 50 mg kg-1d-1administration of Cantide compared to the saline group in a dose-dependent manner, which included injecting Cantide 2. mg kg-1d-1-75 mg kg-1d-1by iv for 2. d after surgically removing the tumor in liver. Cantide was also found to prevent tumor recurrence in the liver and metastasis in the lung, showing a dose-dependent response. When Cantide was administered by iv combined with 5-fluorouracil, it resulted in a significant reduction in tumor growth compared to either agent alone treatment group. After the treatment with Cantide alone or combined with 5-fluorouracil, plasma AFP concentration decreased in a dose-dependent manner. Conclusion: These results demonstrated that Cantide was an effective antitumor antisense oligonucleotide in vivo and has the potential to be developed into a clinical anti-cancer drug.

关键词： telomerase Cantide hepatocellular carcinoma recurrence neoplasm metastasis

Structure Relaxation of Mg_(65)Cu_(2.)Gd_(10) Metallic Glass and Its Effect on Strength

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Journal of Wuhan University of Technology(Materials Science) 2009年第2期24卷 210-213页

作者：李国强 School of Materials Science & Engineering Beijing University of Aeronautics and Astronautics china Perfect Machinery Industry Corp. Ltd

To identify the re-arrangement of constituent atoms of an amorphous Mg65Cu2.Gd10 alloy happened with annealing, structure relaxation of the alloy was investigated as a function of an- nealing time at 373 K through extended X-ray absorption fine structure （EXAFS） analysis procedures. To understand the effect of structure relaxation on strength, compression tests were conducted for both the as-cast and the annealed Mg65Cu2.Gd10 samples. It is found that short range order around Cu and Gd atoms exhibits different variation trends with increasing annealing time at 373 K, though the structure of the alloy still remains to be amorphous. Based on the fact that the strength of the alloy first exhibits a reduction and then a recovery with annealing time, it is suggested that the enhancement of short range order around Cu should be responsible for the strength reduction, while the enhancement of short range order around Gd should be responsible for the strength recovery.

关键词： Mg-based metallic glass structure relaxation strength annealing

Effect of Cycloheximide on the Adaptive Response Induced by Low Dose radiation

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Biomedical and Environmental Sciences 1992年第1期5卷 46-52页

作者： CAI Lu AND LIu SHUZHENGDepartment of radiation Biology, institute of radiation medicine, Norman Bethune University of Medical Sciences, Changchun 13002., china Corresponding author Department of radiation Biology Norman Bethune University of Medical Sciences Changchun China

Human lymphocytes pre-exposed to 10 mGy or 50 mGy of X-rays become less sensitive to subsequent large dose irradiation, exhibited lower rate of chromosome aberration than expected. This adaptive response could be inhibited by cycloheximide, a protein synthesis inhibitor for successive 2.h period ranging from 0.5h before to 4h after the low dose exposure, indicating that the adaptive response was directly related with the protein synthesis.

关键词： In Effect of Cycloheximide on the Adaptive Response Induced by Low Dose radiation

人脑红蛋白(NGB)全长cDNA序列的克隆

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生物化学与生物物理进展 2002年第6期29卷 946-951页

作者：王春丽张成岗军事医学科学院放射医学研究所北京100850

人脑红蛋白 (neuroglobin ,NGB)是新发现的神经系统特异的携氧蛋白 ,然而其全长cDNA序列一直未见报道 .采用电子序列延伸技术和cDNA序列末端快速扩增技术 (rapidamplificationofcDNAends ,RACE)研究发现 ,人NGB全长cDNA序列为 190 9bp,... 详细信息

人脑红蛋白 (neuroglobin ,NGB)是新发现的神经系统特异的携氧蛋白 ,然而其全长cDNA序列一直未见报道 .采用电子序列延伸技术和cDNA序列末端快速扩增技术 (rapidamplificationofcDNAends ,RACE)研究发现 ,人NGB全长cDNA序列为 190 9bp,5′非编码区为 375bp ,编码区 (45 6bp)可编码 15 1个氨基酸 ,3′非编码区为 10 78bp,其中含 2.7bp的 poly (A) (GenBank接受号 :AF4 2.2.797) .综合采用电子序列延伸技术与RACE技术是获得全长cDNA序列的有效方法 ,为后续的功能研究提供了重要基础 .

关键词：人脑红蛋白全长cDNA序列克隆电子序列延伸技术 cDNA末端快速扩增技术

Source time-function as discriminant using empirical Green's function

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Science china Earth Sciences 2005年第12期48卷 2118-2122页

作者： HE Yongfeng1,2.& CHEN Xiaofei1 1. Department of Geophysics, School of Earth & Space Sciences, Peking University, beijing 100871, china 2. North-West {2. of Nuclear Technology, Xi’an 71002., china Department of Geophysics School of Earth & Space Sciences Peking University Beijing China North-West institute of Nuclear Technology Xi’an China

The source mechanism of underground nuclear explosions is remarkably different from that of earthquakes in both spatial radiation pattern and source time-function (STF). Small underground nuclear explosions can be used as empirical Green’s functions (EGF) to isolate the source-time spectrum of a large suspected earthquake occurred in a Nuclear Test Site (target area) by the spectral division method. As this study shows, with high-quality data, the quotient spectrum can be transformed to the time domain, yielding the apparent far-field source-time function of the large suspected event. The relative source time-function (RSTF) of a nuclear ex-plosion is usually a simple pulse with a duration of about 1 s., while an earthquake’s is more complicated with a series of pulses and a longer duration. The RSTF can be used as a nice dis-criminant to distinguish the nature earthquakes from underground nuclear explosions in target areas.

关键词： target-area, empirical Green's function, RSTF.

Tungsten-182.Mssbauer experiments at liquid nitrogen temperature

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Chinese Science Bulletin 2001年第9期46卷 732-734页

作者： HUANG Runsheng, LIU Bing, ZHANG Jinrong, LIU Cong & GAO Xuekui1. Physics Department of Nanjing University, Nanjing 2.0093, china 2. china {2. of Atomic Energy, beijing 102.13, china Physics Department of Nanjing University Nanjing China china institute of Atomic Energy Beijing China

A good 182.a Mossbauer source and an apparatus designed specially were made. 182. transmission Mossbauer experiments were successfully carried out, both of the source and absorber at liquid nitrogen temperature. It wa... 详细信息

关键词： Mossbauer effect 182. Mossbauer effect.

Radioimmunotherapy of carcinoma of colon with [131I]-labeled recombinant chimeric monoclonal antibodies to carcinoembryonic antigen

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Acta Pharmacologica Sinica 2005年第10期26卷 1259-1264页

作者： Qiu-jun LU~1, Guang-xing BIAN, Yuan-yuan CHEN, Min ZHANG, Shao-ming GUO, Li-qing WEN institute of radiation {2., beijing 100850, china instituteofradiation{2. Beijing100850China

Aim: To study the distribution of [131I]-labeled anti-CEA MoAbs and its therapeutic effect on the human colonic cancer model in nude mice. Methods: A nude mice model of human colonic cancer was established. [~131I]-labeled anti-CEA MoAbs were injected intravenously into mice. The distribution of the MoAbs was then determined and the effect of RIT on human colonic cancer was observed. Results: The [131I]-labeled anti-CEA MoAbs had a specific distribution after injection. Tumor/non-tumor ratios for [131I]-labeled anti-CEA MoAbs were 10-2. times higher than [131I]-labeled IgG 96 h after injection. Thirty days after injection, significant inhibition of the volume and weight of tumor was observed in the treated mice compared with the control. The tumor growth inhibition rate of 3,1 mCi/kg CEA MoAbs group (LS180, LS174T, SW1116) was 47.8%～64.0%. This was 69.6%-78.6% in the 6.2. mCi/kg CEA MoAbs group, and 81.8%-86.2. in the 12.5 mCi/kg [131I]-labeled anti-CEA MoAbs group. The plasma CEA level was alo lower in treated mice. Conclusion: The results indicate that [131I]-labeled anti-CEA MoAbs can be effective in RIT on colonic cancers.

关键词： humanized chimeric recombinant monoclonal antibody radioimmunotherapy colonic cancer nude mice

Inertial theory of the mean Hadley circulation under the condition of nonstatic equilibrium

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Science china Earth Sciences 2006年第5期49卷 546-553页

作者： CHAO Jiping1 & LIU Fei2.3 1. National Marine Environmental Forecasting Center, beijing 100081, china 2. Key Laboratory Regional Climate-Environment for Temperate East Asia, {2. of Atmospheric Physics, Chinese Academy of Sciences, beijing 10002., china 3. Graduate University of Chinese Academy of Sciences, beijing 100049, china National Marine Environmental Forecasting Center Key Laboratory Regional Climate-Environment for Temperate East Asia Institute of Atmospheric Physics Chinese Academy of Sciences Graduate University of Chinese Academy of Sciences

Tropical atmosphere is divided into two layers, the layer near the ground is the planet boundary layer, where the motion is linear, if the radiation equilibrium temperature is given at the top of the boundary layer, the vertical motion there can be obtained. It is the non-viscous ideal atmos- pheric layer above the boundary one, where the motion is nonlinear. After getting the conservation laws of angular momentum, potential temperature and potential vortex, the inertial theory of the Hadley circulation driven by the vertical motion of the boundary layer is established, followed by sev- eral examples of its application.

关键词： Hadley circulation, inertial theory, conservation laws of angular momentum, potential temperature and potential vortex.