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Identification and characterization of a novel isoform of hepatopoietin

第四届全国RNA进展研讨会

作者： Han-Jiang Fu, Jie Zhu, Ming Yang, Zhuo-Yuan Zhang, Hong Jiang, Yi Tie, Zhi-Xian Sun, Xiao-Fei Zheng beijing institute of {2. medicine, beijing 100850, china

The microRNAs (miRNAs) are an extensive class of small noncoding RNAs (18 to 2. nucleotides) with probable roles in the regulation of gene expression. Although a large number of miRNAs have been reported in a variety of eukaryotic systems, the function of the vast majority of these molecules remains to be determined. To study the functions of miRNAs, it is very important to define the cell and tissue specificity,

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World Journal of Gastroenterology 2002年第2期8卷 353-356页

作者： Jun Lu Wei-Min Cai,institute of Infectious Disease,First Affiliated Hospital,Medical SchooI,Zhejiang University,Hangzhou 310003 china Wang-Xiang Xu Yi-Qun Zhan Xiao-Lin Cui Fu-Chu He Xiao-Ming Yang,institute of radiation medicine,Academy of Military Medical Sciences,beijing 100850,china institute of infectious Disease First Affiliated Hospital Medical School Zhejiang University Hangzhou 310003 China.

AIM: To isolate a novel isoform of human HPO (HPO-2.5) from human fetal liver Marathon-ready cDNA and characterize its primary biological function. METHODS: 5'-RACE (rapid amplification of cDNA 5' ends) was used to isolate a novel isoform of hHPO in this paper. The constructed pcDNA(HPO-2.5), pcDNA(HPO) and pcDNA eukaryotic expression vectors were respectively transfected by lipofectamine method and the stimulation of DNA synthesis was observed by (3)H-TdR incorporation assay. Proteins extracted from different cells were analyzed by Western blot. RESULTS: A novel isoform of hHPO (HPO-2.5) encoding a 2.5 amino acid ORF corresponding to a translated production of 2. kDa was isolated and distinguished from the previous HPO that lacked the N-terminal 80 amino acids. The dose-dependent stimulation of DNA synthesis of HepG2.hepatoma cells by HPO-2.5 demonstrated its similar biological activity with HPO in vitro. The level of MAPK (Mitogen-activated protein kinase) phosphorylation by Western blot analysis revealed that HPO-2.5 might have the stronger activity of stimulating hepatic cell proliferation than that of HPO. CONCLUSION: A novel isoform of hHPO (HPO-2.5) was isolated from hepatic-derived cells. The comparison of HPO-2.5 and HPO will lead to a new insight into the structure and function of hHPO, and provide the new way of thinking to deeply elucidate the biological roles of HPO/ALR.

关键词： Amino Acid Sequence Cell Division Cell Fractionation Enzyme Activation Hepatocyte Growth Factor purification Humans Liver Mitogen-Activated Protein Kinases Molecular Sequence Data Open Reading Frames Protein Isoforms Sequence Alignment Tumor Cells, Cultured

Inter-comparisons of thermodynamic sea-ice modeling results using various parameterizations of radiative flux

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Acta Oceanologica Sinica 2006年第1期25卷 21-31页

作者： ZHANG Zhanhai CHENG Bin LAUNIAINEN Jouko WU Huiding LIU Yu State Oceanic Administration Key Laboratory for Polar Science Polar Research Institute of China Shanghai 200136 China Finnish institute of Marine Research Helsinki Fin-00931 Finland National Marine Environment Forecast Center Beijing 100081 China

Radiative fluxes are of primary importance in the energy and mass balance of the sea-ice cover. Various parameterizations of the radiative fluxes are studied in a thermodynamic sea-ice model. Model outputs of the surface radiative and heat fluxes and mass balance are compared with observations. The contribution of short-wave radiation is limited to a long part of winter. Therefore, simple schemes are often sufficient. Errors in estimations of the short-wave radiation are due mainly to cloud effects and occasionally to multi-reflection between surface and ice crystals in the air. The long-wave radiation plays an important role in the ice surface heat and mass balance during most part of a winter. The effect of clouds on the accuracy of the simple radiative schemes is critical, which needs further attention. In general, the accuracy of an ice model depends on that of the radiative fluxes.

关键词： parameterizations of radiative fluxes energy balance at the surface of ice/snow thermodynamic sea-ice model Baltic Sea Bohai Sea

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PERIODIC TABLE OF CLIMATIC CHANGES

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海洋地质与第四纪地质 1992年第2期 47-51页

作者： Xu Qinqi(institute of Vertebrate Paleontology and Paleoanihropology, Academia Sinica, beijing, 100044,{2.)Huang Yuzhen(beijing Municipal Computing Centre, beijing, 100005,{2.)

The climate changed regularly in accordance with the variation of the average incoming solar radiation in the winter half-year of the northern hemisphere on the Earth’s surface in each oxygen isotopic stage (i. e. Di in Table 1) during the last 730,000 years. Many interesting phenomena of the nature may be explained with applications of these data.

关键词： astroclimatology oxygen isotopic stage the penultimate glacial Peking Man

Proteomic analysis reveals the radiosensitization of vanillin derivative bromovanin by targeting G2.M checkpoint and mitotic catastrophe

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Proteomic analysis reveals the radiosensitization of vanilli...

An experimental study on variation of thermal fields during the deformation of a compressive en echelon fault set

中国毒理学会第五次全国学术大会

作者： Bo Zhang,Shu-Bing Wang,Qin-Zhi Xu,Xiao-Dan Liu,Ping-Kun Zhou beijing institute of {2. medicine,AMMS,beijing 100850

＜正＞AIM To study the radiosensitizing effect and the related mechanism of the novel vanillin derivative bromovanin,and provide evidence and experimental data for exploring it as new anticancer drug. Method Hepatic c...

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Progress in Natural Science:Materials International 2007年第3期17卷 298-304页

作者： LIU Peixun, MA Jin, LIU Liqiang, MA Shengli and CHEN Guoqiang (Laboratory of Earthquake Dynamics, institute of Geology, china Earthquake Administration, {2. 10002., china) Laboratory of Earthquake Dynamics Institute of Geology China Earthquake Administration Beijing 100029 China

The temperature variations during deformation of a compressive en echelon fault set in a rock sample were measured by a surface measurement system with multi-point platinum resistance thermometers and an infrared thermal image system. The measurements obtained by the two systems were in agreement with each other, indicating a relationship between temporal-spatial variations of the thermal field and changes of stress and strain. At varied structural positions of the rock sample, the rising of the temperature was different, implying distinct stress distributions at these positions. In the experiment, the deformation process of the sample included three stages: elastic deformation, stick-slip, and rupture. Correspondingly, the variation process of temperature had also three stages, each of which had its own temperature rising profile. And the thermal radiation field showed a similar process. These phenomena mean that the dominant mechanisms of temperature rising in all stages of deformation are different. Experimental results provide a physical basis for the study of current fault activities by using data of satellite infrared images.

关键词： en echelon fault set stick-slip thermal infrared radiation temperature mechanism of temperatnre rising.

病原DNA识别及其诱导天然免疫调节机制研究进展

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生物化学与生物物理进展 2011年第12期38卷 1099-1105页

作者：邢雅玲郑洋王凯陈晓娟陈忠斌军事医学科学院放射与辐射医学研究所北京100850

机体天然免疫系统拥有一系列可以探测和抵制微生物侵袭的机制.目前,关于病原RNA的细胞内识别机制有了较为深入的研究和相关报道,但细胞内病原DNA的识别和相应的天然免疫应答机制仍未完全被揭示.阐明上述机制有助于了解和治疗一系列微生... 详细信息

机体天然免疫系统拥有一系列可以探测和抵制微生物侵袭的机制.目前,关于病原RNA的细胞内识别机制有了较为深入的研究和相关报道,但细胞内病原DNA的识别和相应的天然免疫应答机制仍未完全被揭示.阐明上述机制有助于了解和治疗一系列微生物感染相关的疾病,包括病毒和细菌感染类疾病、病毒相关的肿瘤、自身免疫性疾病等.近年来,细胞内多个充当"DNA传感器"的分子和干扰素调节分子被认为在细胞质DNA诱导宿主天然免疫反应过程中起着关键性调节作用.综述了对细胞内病原DNA的主要识别分子、信号通路以及相关的天然免疫调控机制.

关键词：病原DNA DNA传感器天然免疫干扰素

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Meteorological observations on Mount Everest in 2.05

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Progress in Natural Science:Materials International 2007年第7期17卷 828-837页

作者： Xie Aihong~(1**) Qin Dahe~1 Ren Jiawen~1 Qin Xiang~1 Xiao Cunde~(1,2. Hou Shugui~1 Kang Shichang~(1,3) Yang Xingguo~(1,4) Jiang Youyan~1 (1.Key Laboratory of Cryosphere and Environment,Cold and Arid Regions Environment and Engineering Research institute,Chinese Academy of Sciences,Lanzhou 730000,china 2.Chinese Academy of Meteorological Science,{2. 100081,china 3.institute of Tibetan Plateau Research,Chinese Academy of Sciences,{2. 100085,china 4.Gansu Provincial Meteorological Bureau,Lanzhou 730000,china) Key Laboratory of Cryosphere and Environment Cold and Arid Regions Environment and Engineering Research Institute Chinese Academy of Sciences Lanzhou 730000 China

Mount Everest,the highest point on the Earth is often referred to as the earth's third pole as such the place is relatively inaccessible and little is known about its *** April 2.05,an automatic weather station was installed at the mountain's North Col(652. m a.s.l.).According to the observational 10-minute mean and daily records,the meteorological characteristics were analyzed. All the meteorological elements displayed obvious diurnal variations during May 1 to July 2.,*** monthly variation of daily meteo- rological elements on Mount Everest coincided with that on Dingri,the closest routine meteorological station,with the high correlation co- efficients of 0.92.,0.877,0.682.0.755,0.82. and 0.676(n=83,p<0.001)for mean temperature,minimum temperature,max- imum temperature,relative humidity,pressure and wind speed,***,the vertical mean gradient of temperature was above 0.6℃/100 m,especially for the daily maximum *** weather events on Mount Everest prominently appeared on the same day as those on Dingri,especially those from daily mean pressure,temperature and relative humidity with the cross-correlation coeffi- cients of 0.673,0.485 and 0.487(n=83,p<0.001),*** other weather events on Mount Everest lagged one-day be- hind those on ***,forecasting of the weather events on Mount Everest from pressure on Dingri was more reliable than those from the other meteorological *** conclusions are much important for research on meteorology and climate changes in the region.

关键词： Mount Everest meteorological observation Dingri County radiation air temperature relative humidity air pressure wind

Pressure-induced crystallite breaking in La_(0.7)Sr_(0.3)Mn_(0.9)Fe_(0.1)O_3 nanosolids

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Chinese Science Bulletin 1998年第6期43卷 458-461页

作者： ZHENG Fanlei 1, 2. SUI Yu 1, 3, XU Dapeng 1 and SU Wenhui 1, 4 1. Department of Physics, Jilin University, Changchun 13002., china 2. Changchun institute of Physics, Chinese Academy of Sciences, Changchun 13002., china 3. institute of Metal R Department of Physics Jilin University Changchun Chinas Changchun institute of Physics Chinese Academy of Sciences Changchun China institute of Metal Research Chinese Academy of Sciences Shenyang China International Center for Materials Physics Chinese Academy of Sceinces Shenyang China Center for Condensed Matter and radiation Physics CCAST (World Lab.) Beijing China

In the compacting process of the La 0.7Sr 0.3Mn 0.9Fe 0.1O 3 nanosolids under the pressure range of 0.0-4.5 GPa, the apparent pressure-induced crystallite breaking phenomenon in these nanosolids was observed. With increasing pressure up to 4.5 GPa, the average grain size decreases by 46% while the magnetization of nanosolids decrease by 40% and their coercive increases by 35%. This kind of breaking has a close relation to the existence of oxygen deficiency in La 0.7Sr 0.3Mn 0.9Fe 0.1O 3 nanoparticles. A simple and convenient method for preparing the bulk nanosolids with a large number of clean interfaces has been suggested.

关键词： nanosolid pressure-induced breaking high pressure.

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