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用脉冲电场凝胶电泳分析棉病囊霉及其突变体的染色体DNA

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生物化学与生物物理进展 1993年第4期20卷 286-288页

作者：杨素红葛忠良北京放射医学研究所北京100850

采用交变脉冲电场凝胶电泳和碱变性交变脉冲电场凝胶电泳方法,分析了棉病囊霉酵母菌及其2.不同的突变菌株的核型,得知此菌株含有5条染色体 DNA,而2.突变体的染色体 DNA 都没有大片段的缺失或双链断裂,但其稳定性不如野生型菌株的 DNA... 详细信息

采用交变脉冲电场凝胶电泳和碱变性交变脉冲电场凝胶电泳方法,分析了棉病囊霉酵母菌及其2.不同的突变菌株的核型,得知此菌株含有5条染色体 DNA,而2.突变体的染色体 DNA 都没有大片段的缺失或双链断裂,但其稳定性不如野生型菌株的 DNA,而且存在单链断裂等碱不稳定性位点.

关键词：棉病囊霉电泳染色体 DNA

用基因芯片检测DPYD等位基因在受试人群中的发生频率

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生物化学与生物物理进展 2004年第11期31卷 1031-1037页

作者：文思远王晓云张敏丽王升启军事医学科学院放射医学研究所北京100850 北京大学第一附属医院北京100009

二氢嘧啶脱氢酶基因 (DPYD基因 )所编码的二氢嘧啶脱氢酶 (DPD酶 )是氟化嘧啶类抗肿瘤药物代谢的主要限速酶 ,其活性存在显著的个体差异 ,并因此影响药物的疗效和毒副作用 .大部分编码低 /无活性酶的突变型等位基因是由于基因中的单核... 详细信息

二氢嘧啶脱氢酶基因 (DPYD基因 )所编码的二氢嘧啶脱氢酶 (DPD酶 )是氟化嘧啶类抗肿瘤药物代谢的主要限速酶 ,其活性存在显著的个体差异 ,并因此影响药物的疗效和毒副作用 .大部分编码低 /无活性酶的突变型等位基因是由于基因中的单核苷酸多态性 (singlenucleotidepolymorphism ,SNP)造成的 ,检测这些SNPs是预测患者对药物的反应和实现个体化给药方案的基础 .制备并优化了用于检测DPYD基因中 6个已知SNPs所编码的等位基因 (DPYD 2., 3, 4 , 5 , 9, 12.)的基因芯片 ,建立了该芯片的基因分型标准 .并利用该芯片检测了肿瘤患者 (112. )、肾病患者 (83例 )和健康者 (4 5例 )中DPYD突变型等位基因的发生频率 .在受试人群中 ,突变型等位基因DPYD 5和DPYD 9平均发生率分别为 32. 0 8%和 11 2.5 % ,未发现DPYD 2., 3, 4 , 12.变型等位基因 .而且以上单碱基突变的发生率在肿瘤患者、肾病患者和健康者间以及男性、女性肿瘤患者间无显著性差异 ,表明其与疾病的发生或性别无显著性关联 .对 2.0例标本的基因分型结果采用直接测序法进行验证 ,19例基因芯片分型结果与直接测序法结果相一致 .DPYD 5、DPYD 9突变型等位基因在受试人群中具有较高的发生率 .利用基因芯片能够对其实现快速准确的检测 .

关键词：突变型等位基因人群肿瘤患者发生率基因芯片肾病发生频率直接测序法利用

Hawking radiation and thermodynamics of a Vaidya-Bonner black hole

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Research in Astronomy and Astrophysics 2010年第1期10卷 33-38页

作者： Zhen-Feng Niu Wen-Biao Liu Department of Physics Hebei North University Zhangjiakou 075000 China Department of Physics Institute of Theoretical Physics Beijing Normal University Beijing 100875 China

Using Parikh＇s tunneling method, the Hawking radiation on the apparent horizon of a Vaidya-Bonner black hole is calculated. When the back-reaction of particles is neglected, the thermal spectrum can be precisely obtained. Then, the black hole thermodynamics can be calculated successfully on the apparent horizon. When a relativistic perturbation is applied to the apparent horizon, a similar calculation can also lead to a purely thermal spectrum. The first law of thermodynamics can also be derived successfully at the new supersurface near the apparent horizon. When the event horizon is thought of as a deviation from the apparent horizon, the expressions of the characteristic position and temperature are consistent with the previous viewpoint which asserts that the thermodynamics should be based on the event horizon. It is concluded that the thermodynamics should be constructed exactly on the apparent horizon while the event horizon thermodynamics is just one of the perturbations near the apparent horizon.

关键词： black hole physics -- relativity -- kinematics and dynamics

A good example of high-energy radiation delay event

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Chinese Science Bulletin 1997年第4期42卷 313-315页

作者： GAN Weiqun and Rieger Erich1 Purple Mountain Observatory, Nanjing 2.0008, china 2. Max-Planck institute of Extraterrestrial Physics, Garching 85740,Germany Purple Mountain Observatory Nanjing China Max-Planck institute of Extraterrestrial Physics Garching Germany

THE delay of high-energy radiation of solar flares can be divided into two types: one is the energy-dependent delay in the hard X-ray regime, the other is the delay between the hard X-rays and instantaneous γ-ray line (GRL) emissions. Both types have different physical meanings. The former reflects the difference in acceleration or transportation of energetic electrons with various energies, while the latter reflects such a difference between the energetic electrons

关键词： sun. flare X-ray γ-ray.

Preliminary results of a regional air-sea coupled model over East Asia

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Chinese Science Bulletin 2010年第21期55卷 2295-2305页

作者： LI Tao ZHOU GuangQing institute of Atmospheric Physics Chinese Academy of Sciences Beijing 100029 China Graduate University of the Chinese Academy of Sciences Beijing 100049 China

We have established a regional air-sea coupled model over East Asia and conducted a 2.-year integration to evaluate its performance in reproducing the present *** coupled model consists of RegCM3 and HYCOM controlled by the OASIS3 coupler with resuolution of 60 km for the atmosphere and 33 km for the ocean,*** some other regional air-sea coupled models,a one-way nesting method is employed in the oceanic component and a heat flux adjustment for solar radiation is used to remove an about 2.C cold bias in *** primary analysis for this 2.-year integration shows that the coupled model successfully reproduces the main features of the circulations over East Asia,both in the atmosphere and the ocean,including climatology,seasonal and interannual *** are seen in the coupled model compared to the uncoupled one,especially in the simulation of precipitation,the most important element of the East Asian monsoon,although there are still obvious discrepancies that come mainly from the model components *** analyses show that the rainfall simulation benefits from the enhancements of the Northwest Pacific Subtropical High in summer,which leads to the improvement of the moisture flux simula-tion at the middle-lower atmospheric *** results indicate that the regional air-sea coupled model is more suitable for the East Asia monsoon simulation.

关键词：海气耦合模式东亚地区空气西北太平洋副高大气环流海域耦合模型模拟降雨

Dynamic study of photosensitized one-electron oxidation of polyG by menadione

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Science china Chemistry 2005年第4期48卷 292-296页

作者： MA Jianhua1,LIN Weizhen2.DU Fuqiang2.HAN Zhenhui2.YAO Side2.& LIN Nianyun2.1.College of Biology Engineering,Jimei University,Xiamen 36102.,china 2.Laboratory of Nanobiomedicine & Laboratory of {2. Chemistry,Shanghai institute of Applied Physics,Chinese Acade Sciences,Shanghai 2.1800,china College of Biology Engineering Jimei University Xiamen 361021 China

The electron transfer reaction of triplet menadione (3MQ?) with polyguanylic acid (polyG) in CH3CN rich (97%) solution was investigated by 2.8 nm(KrF) laser flash *** transient absorption spectra and kinetics obtained from the interaction of 3MQ? with polyG demonstrated that the primary radical ion pair,radical cation of polyG and radical anion of me-nadione can be detected *** results demonstrated that the reaction rate con-stant of 3MQ? with polyG is almost equal to that of 3MQ? with dGMP.

关键词： menadione, polyG, laser flash photolysis, triplet state, one electron oxidation.

Inhibitor of MAPK/ERK kinase but not NMDA receptor antagonist blocks the survival-promoting effect of learning on immature neurons in hippocampus

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Inhibitor of MAPK/ERK kinase but not NMDA receptor antagonis...

中国神经科学学会第九届全国学术会议暨第五次会员代表大会

作者： Zhi-Wei XU**,Yong-Hong WU**,Fei-Long Zhang,Yan GAO,Cheng-Gang ZHANG~* beijing institute of {2. medicine,State Key Laboratory of Proteomics,beijing 100850,china

＜正＞There is growing evidence demonstrating that hippocampus-dependent learning can rescue the newly generated neurons from apoptotic cell death,but the underlying mechanism is largely *** we explored the role of ERK signaling pathway in survival-promoting effect of hippocampus-dependent *** were injected intraperitoneally with bromodeoxyuridine（BrdU） to label dividing *** week later,the mice were trained in Morris water maze for five consecutive ***32.（30 mg/kg）,an inhibitor of the upstream MAPK/ERK kinase （MEK） and MK801（0.14 mg/kg）,an antagonist of NMDA receptor were injected 30 min respectively before training. Level of extracellular signal-regulated kinase（ERK） phosphorylation was characterized using Western *** of BrdU-labeled cells in dentate gyrus was then *** showed that spatial learning enhanced the survival of immature neurons generated before ***32. did not produce significant impairment in escape latency of spatial learning,although SL32. down-regulated levels of hippocampal ERK ***,SL32. blocked the survival-promoting effect of *** contrast,MK801 increased the phosphorylation levels of ERK in hippocampus of the ***,MK801 also disrupted the performance of mice on spatial learning and decreased swimming speed of the ***,MK801 did not modulate survival-promoting effect of learning. Taken together,our results demonstrate that learning tasks enhance survival of new neurons through ERK-dependent signaling mechanisms but not mediated by activation of NMDA receptor in *** current study might provide a possible regulatory strategy of compensating neurogenesis losses associated with neurodegeneration disease and nonpathological ageing.

关键词： neurogenesis cell survival learning ERK NMDA receptor

来源： cnki会议评论

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cnki会议

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用掩蔽注入法研究钛注入H13钢的耐磨性

物理学报 2004年第11期53卷 3823-3828页

作者：杨建华张通和南通工学院物理部南通226007 北京师范大学射线束和材料工程教育部重点实验室北京100875

采用由金属蒸汽真空弧离子源引出的强束流钛、碳离子对H13钢进行表面改性研究 .钛和碳离子注入剂量分别为 3× 10 1 7和 1× 10 1 7cm- 2.,引出电压分别为 4 8和 30kV ,平均束流密度分别为 4 7和 2.0 μA·cm- 2..为了保持相同的摩擦磨... 详细信息

采用由金属蒸汽真空弧离子源引出的强束流钛、碳离子对H13钢进行表面改性研究 .钛和碳离子注入剂量分别为 3× 10 1 7和 1× 10 1 7cm- 2.,引出电压分别为 4 8和 30kV ,平均束流密度分别为 4 7和 2.0 μA·cm- 2..为了保持相同的摩擦磨损实验条件 ,注入过程中采用掩蔽注入技术 .摩擦磨损实验结果表明 ,钛离子注入H13钢提高了其耐磨性 ,并大幅度降低其摩擦系数 .利用卢瑟福背散射谱测量了离子注入表面的成分 ,并采用逐层递推法得出了钛在H13钢中的浓度深度分布。

关键词： H13钢耐磨性表面相结构背散射金属摩擦磨损束流掩蔽递推法

Data　Bank　and　Consulting　System　for　Medical　Management　of　Patiens　with　Acute　radiation　Sickness

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中华放射医学与防护杂志 1995年第5期 306-310页

作者： Tang Zhongming Ye Genyao Mao Bingzhi Luo Qingliang Wang Guilin Liu Xiuwen Lin Ke and Wu Dechang(beijing institute of {2. Medicime,beijing 00850,P.R.china)(North Taipmg Read Hospital beijing 100039,P.R. china)

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关键词： Software data bank consultation medical treatment acute radiation syndrome