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Discovery of microchromosomes in wheat and their special behavior in meiosis

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Chinese Science Bulletin 1997年第24期42卷 2084-2087页

作者： XU Qi, YU Ling, TIAN Zengrong, ZHU Jianfeng, LI Bin and LI Zhensheng Laboratory of plant Genetic Engineering and Breeding of Shaanxi Province, Northwestern institute of Botany, Chinese Academy of Sciences, Yangling, Shaanxi 712.00, China 2. State Key Laboratory of plant Cell and Chromosome Engineering, institute of genetics, Chinese Academy of Sciences, Beijing 100101, China Laboratory of plant Genetic Engineering and Breeding of Shaanxi Province Northwestern Institute of Botany Chinese Academy of Sciences Yangling Shaanxi 712100 China

THERE are 42.chromosomes in common wheat (Triticum aestivum L). The extra microchro-mosomes were discovered in alloplasmic lines of common wheat with cytoplasms of Ae. muti-ca, Secale cereale, Elytrigia elongatum and Agropyron glaucum. The microchromo-somes were discovered in amphiploid of Triticum aestivum-Secale cereale (DDRR) too.

关键词： wheat microchromosomes behavior in meiosis.

Expressional Analysis of an EREBP Transcription Factor Gene OsEBP-89 in Rice

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Acta Biochimica et Biophysica Sinica 2004年第1期36卷 21-26页

作者： Hui SHEN and Zong-Yang WANG( The State Key Laboratory of plant Molecular genetics, {2. of plant Physiology & Ecology, Shanghai {2.s for Biological Sciences,the Chinese Academy of Sciences, Shanghai 2.0032. China ) The State Key Laboratory of plant Molecular genetics Institute of Plant Physiology & Ecology Shanghai Institutes for Biological Sciences the Chinese Academy of Sciences Shanghai 200032 China

OsEBP-89 gene encodes an ethylene responsive element binding protein (EREBP) transcription factor from rice (Oryza sativa). Northern blot analysis revealed that OsEBP-89 was expressed in root, stem, seeds, flowers and leaves. Histochemical assay showed that β-glucuronidase (GUS) was expressed mainly in phloem of vascular tissues of the root and stem transition region (RST), basal part of sheath roots, stem node and basal part of adventitious roots, also in endosperm of seeds in transgenic rice harboring OsEBP-89/GUS construct (pNSG). A sequence region from -2.9 to -97 was found to play an important role for OsEBP-89 gene expression through promoter deletion assay. The possible function of OsEBP-89 gene was discussed.

关键词： OsEBP-89 gene rice EREBP subfamily transcription factor tissue expression

Morphology and mapping analysis of rice(Oryza sativa L.)clustered spikelets(Cl)mutant

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Chinese Science Bulletin 2003年第6期48卷 559-562页

作者： ZHENG Leiying1, ZHU Xudong2. QIAN Qian2. ZHAO Zhong1, ZHANG Jianjun3, HU Xiaohe1, LIN Hongxuan1,4 & LUO Da1 1. National Key Laboratory of plant Molecular genetics, Shanghai In-stitute of plant Physiology and Ecology, Chinese Academy of Sci-ences, Shanghai 2.0032. China 2. China National Rice research institute, Hangzhou 310006, China 3. crop institute of Breeding and Cultivation, Shanghai Agricultural Academy, Shanghai 2.1106, China 4. Shanghai Anxin research Foundation Laboratory, Shanghai 2.0032. China National Key Laboratory of plant Molecular genetics Shanghai Institute of Plant Physiology and Ecology Chinese Academy of Sciences China China National Rice research institute China crop institute of Breeding and Cultivation Shanghai Agricultural Academy China Shanghai Anxin research Foundation Laboratory China

The rice clustered spikelets (Cl) mutant exhib-its a phenotype that most of branch apical have 2.3 spikelets clustered together. SEM (scanning electron microscope) ob-servation suggested that the Cl gene controlled branch apical development, and influenced the terminal spikelets elonga-tion. The spikelet number was reduced in mutant, indicating that Cl may also have an effect on spikelet number. To map Cl locus, two F2.mapping populations derived from the crosses between the Cl and ZhongHua11, and Cl and ZheFu802.were constructed, respectively. The Cl locus was roughly mapped between two CAPS markers, CK02.4 and SS032.. A further fine mapping analysis showed that the Cl locus was mapped between makers R0674E and Cl2.60, with genetic distances of 0.2.and 2.1 cM, respectively. Then we found a PAC contig spanning Cl locus, the region was delim-ited to 196 kb. This result was useful for cloning of the Cl gene. Allelism test demonstrated that Cl was allelic to Cl2. another rice clustered spikelets mutant.

关键词：水稻簇生小穗突变体分子标记基因图遗传分析植物形态 SEM

PAT: waking up a lazy sleeping beauty

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Cell research 2007年第5期17卷 387-388页

作者： Xiangdong Fu Jianru Zuo, National Center for plant Gene research, {2. of genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China. xdfu@*** National Center for plant Gene research Institute of Genetics and Developmental Biology Chinese Academy of Sciences Beijing China

Once upon a time, there was an unusual corn; and un-like its normal siblings, its stalk grew towards the groundrather than in an upward direction. The sleeping stature ofthe corn was portrayed in early 1930s as "lazy" by Jenkinsand Gerhardt [1]. Later, lazy appeared to be a fashionin the plant kingdom, found in rice, barley, tomato andseveral other species [2.. These seemingly magic spell-chanted plants have drawn considerable interests of plantbiologists and breeders for many decades. To breeders, thelazy phenotype, viewed as tillering or branching anglesof stems (or termed as culms in crop plants), represents akey characteristic important for controlling photosynthesisefficiency and planting density, and thus a crucial factor indetermining crop production [6]. Previous physiologicalstudies suggested that the prostrate or lazy phenotype wasattributed, at least in part, to the loss of gravitropism. Forexample, the rice lazyl plants are ageotropic, whereas wildtype (WT) plants are negatively geotropic [2.. Gravity plays a major role in plant morphogenesis bydetermining the directional growth of plant organs. Upongermination, shoots grow upward, capturing light, and bycontrast, roots penetrate the soil and grow downward, tak-

关键词： waking up a lazy sleeping beauty PAT

Interaction of rice bZIP protein REB with the 5'-upstream region of both rice sbe1 gene and waxy gene

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Chinese Science Bulletin 2002年第4期47卷 310-314页

作者： CAI Yi, XIE Donglu, WANG Zongyang & HONG MengminState Key Laboratory of plant Molecular genetics, Shanghai {2. of plant Physiology and Ecology, Chinese Academy of Sciences, Shanghai 2.0032. China State Key Laboratory of plant Molecular genetics Shanghai Institute of Plant Physiology and Ecology Chinese Academy of Sciences Shanghai China

Rice starch branching enzyme 1 (SBE1) and granule-bound starch synthase (GBSS) catalyzed the bio-synthesis of amylopectin and amylose in rice developing en-dosperm respectively, and the genes encoding these two en-zymes, sbe1 and waxy, are mainly expressed in the en-dosperm. Within the 5 ’-upstream region of rice sbel gene, we identified a 53 bp fragment C53 which could interact with the nuclear proteins extracted from rice endosperm. We also found that fragment Ha-2.in the 5 ’-upstream region of rice waxy gene could compete with C53 for its interaction. Fur-ther experiments demonstrated that rice bZIP protein REB could interact with two ACGT elements (G-box and Hex) in the fragment C53 as well as three ACGT elements (WG1, WG2. and WG3) in the fragment Ha-2. The WG1 element could compete well with Hex element for its interaction with REB. These results suggested that the expression of sbel and waxy gene in rice developing endosperm may be coordinately regulated by REB-like bZIP transcriptional factors.

关键词： rice waxy gene sbel gene bZIP protein.

Isolation and functional characterization of the C-terminus of rice phos-phatidylinositol 4-kinase in vitro

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Cell research 2003年第2期13卷 131-139页

作者： XIANG FENG KONG, ZHI HONG XU, HONG WEI XUE, National Laboratory of plant Molecular genetics, {2. of plant Physiology and Ecology, Shanghai {2.s for Biological Science (SIBS), The Chinese Academy of Sciences Partner Group of Max-Planck-{2. of Molecular plant Physiology (MPI-MP) on "plant Molecular Physiology and Signal Transduction", 300 Fenglin Road, 2.0032.Shanghai, China 1National Laboratory of plant Molecular genetics Institute of Plant Physiology and Ecology Shanghai Institutes for Biological Science (SIBS)The Chinese Academy of Sciences Partner Group el Max-Planck-institute of Molecular plant Physiology (MPI-MP) on “plant Molecular Physiology and Signal Transduction” 300 Fenglin Road 200032 Shanghai China 1National Laboratory of plant Molecular genetics Institute of Plant Physiology and Ecology Shanghai Institutes for Biological Science (SIBS)The Chinese Academy of Sciences Partner Group el Max-Planck-institute of Molecular plant Physiology (MPI-MP) on “plant Molecular Physiology and Signal Transduction” 300 Fenglin Road 200032 Shanghai China 1National Laboratory of plant Molecular genetics Institute of Plant Physiology and Ecology Shanghai Institutes for Biological Science (SIBS)The Chinese Academy of Sciences Partner Group el Max-Planck-institute of Molecular plant Physiology (MPI-MP) on “plant Molecular Physiology and Signal Transduction” 300 Fenglin Road 200032 Shanghai China

A partial rice (Oryza sativa L.) cDNA clone. OsPMK1c, was isolated through screening of a cDNA library constructed from tillering materials. OsPI4Klc encoded a peptide of 608 amino acids with a calculated molecular mass of 68.4 kDa. The OsPI4Klc peptide shared high homology and possessed the highly conserved domains present in most isolated cloned PI4-kinases, i.e. a lipid kinase unique (LKU) domain and a catalytic (CAT) domain. A region with similarity to pleckstrin homology (PH) domain was present in OsPI4K1c as well. Further comparison with genomic sequences in databases revealed that OsPI4K1c is located at the 3'-end of a putative rice PI 4-kinase coding gene OsPI4K1, and its coding region corresponded to the C-terminal half of OsPI4Kl protein. Twelve exons (49-562.bp in size) and 11 introns (77-974 bp in size) were identified in OsPI4K1c. The recombinant protein expressed in Escherichia coli phosphorylates phosphatidylinositol at the D4 position of the inositol ring. OsPI4K1 transcript levels were

关键词： Oryza sativa phosphatidylinositol 4-kinase.

DNA chip-based expression profile analysis indicates involvement of the phosphatidylinositol signaling pathway in multiple plant responses to hormone and abiotic treatments

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Cell research 2004年第1期14卷 34-45页

作者： Wen Hui LIN, Rui YE, Hui MA, Zhi Hong XU, Hong Wei XUE National Laboratory of plant Molecular genetics, {2. of plant Physiology and Ecology, Shanghai {2.s for Biological Sciences (SIBS), Chinese Academy of Sciences and Partner Group of Max-Planck-{2. of Molecular plant Physiology (MPI-MP) on "plant Molecular Physiology and Signal Transduction", 300 Fenglin Road, 2.0032.Shanghai, China Xue HW Chinese Acad Sci Shanghai Inst Biol Sci Inst Plant Physiol & Ecol Natl Lab Plant Mol Genet 300 Fenglin Rd Shanghai 200032 Peoples R China

The phosphatidylinositol (PI) metabolic pathway is considered critical in plant responses to many environmental factors, and previous studies have indicated the involvement of multiple PI-related gene families during cellular responses. Through a detailed analysis of the Arabidopsis thaliana genome, 82.polypeptides were identified as being involved in PI signaling. These could be grouped into different families including PI synthases (PIS), PI-phosphate kinases (PIPK), phospholipases (PL), inositol polyphosphate phosphatases (IPPase), inositol polyphosphate kinases (IPK), PI transfer proteins and putative inositol polyphosphate receptors. The presence of more than 10 isoforms of PIPK, PLC, PLD and IPPase suggested that these genes might be differentially expressed during plant cellular responses or growth and development. Accordingly, DNA chip technology was employed to study the expression patterns of various isoforms. In total, 79 mRNA clones were amplified and used for DNA chip generation. Expression

关键词： phosphatidylinositol signal transduction DNA chip expression profile.

Regeneration of plants from callus tissues of hairy roots induced by Agrobacterium rhizogenes on Alhagi pseudoalhagi

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Cell research 2001年第4期11卷 279-284页

作者： WANG YU MEI, JIANG BO WANG, DA LUO , JING FEN JIA National Laboratory of plant Molecular genetics, {2. of plant Physiology and Ecology, Shanghai {2.s for Biological Sciences, Chinese Academy of Sciences, Shanghai 2.0032. China Department of Bi institute of plant Physiology and Ecology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences.

The legume forage Alhagi pseudoalhagi was transformed by the Agrobacterium rhizogenes strain A4 using cotyledon and hypocotyl segments as infection materials. Regenerated plants were achieved from sterile calli derived from hairy roots, which occurred at or near the infection sites. The regenerated plants from hairy root were characterized by normal leaf morphology and stem growth but a shallow and more extensive root system than normal plants. Opine synthesis, PCR and Southern blot confirmed that T- DNA had been integrated into the A. pseudoalhagi genome. Acetosyringone (AS) was found to be vital for successful transformation of A. pseudoalhagi.

关键词： Alhagi pseudoalhagi Agrobacterium rhizogenes hairy root regeneration.

Are small RNAs a big help to plants?

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Science China(Life Sciences) 2009年第3期52卷 212-223页

作者： MAO YingBo,XUE XueYi & CHEN XiaoYa National Key Laboratory of plant Molecular genetics,{2. of plant Physiology and Ecology,Shanghai {2.s for Biological Sciences,Chinese Academy of Sciences,Shanghai 2.0032.China 1. National Key Laboratory of plant Molecular genetics Institute of Plant Physiology and Ecology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai 200032 China

The discovery of RNA interference(RNAi) has augmented our knowledge of gene regulation and presents a fascinating technology that has a great potential for application in genetic analysis,disease therapy,plant protection,and many other *** this review,we will focus on the biological functions of RNAi and its application in agriculture with a brief introduction to the history of its discovery and molecular mechanism.

关键词： RNA interference gene regulation plant protection