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Statistical analysis on adaptive evolution of SQUA genes in angiosperms

Progress in Natural Science:Materials International 2005年第1期 94-97页

作者： CHEN Yongyan 1.2. ZHONG Yang 3*, TIAN Bo 1. YANG Ji 4 and LI Dezhu 1.*(1. Laboratory of Plant Biodiversity and Biogeography, Kunming {1. of Botany, Chinese Academy of Sciences, Kunming 6502.4,{1. 2. College of Bioengineering, Dalian University, Dalian 1.662.,{1. 3. Ministry of Education Key Laboratory for Biodiversity and Ecological Engineering, School of Life Science, Fudan University, Shanghai 2.0433,{1. 4. College of Life Sciences, Peking University, beijing 1.0871.{1.) Laboratory of Plant Biodiversity and Biogeography，Kunming institute of Botany，Chinese Academy of Sciences，Kunming 650{1.4 China College of Bioengineering，Dalian University，Dalian 1.662.，{1. Ministry of Education Key Laboratory for Biodiversity and Ecological Engineering，School of Life Science，Fudan University，Shanghai 2.0433，{1. Laboratory of Plant Biodiversity and Biogeography，Kunming institute of Botany，Chinese Academy of Sciences，Kunming 650{1.4 China College of Life Sciences，Peking University，beijing 1.0871.{1.

SQUAMOSA (SQUA) subfamily includes important perianth identity genes of MADS-box gene family. SQUA genes of Dendrocalamus latiflorus were sequenced, and phylogenetic form on SQUA genes in angiosperms was analyzed. Relative rate and adaptive evolution after SQUA gene duplication in recent common ancestor of monocots and eudicots were analyzed using the methods of relative rate test, statistic on synonymous and non-synonymous coden substitution sites and likelihood rate test. The results show that both of relative rate and synonymous and non-synonymous coden substitution in eudicot clade are significantly higher than those in monocot clade, and the value of d N/d S uncovered possible positive selective pressure in eudicot clade.

关键词： angiosperms Dendrocalamus latiflorus SQUA gene relative rate test likelihood rate test adaptive evolution.

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Palaeogeothermal response and record of Late Mesozoic lithospheric thinning in the eastern North china Craton

Palaeogeothermal response and record of Late Mesozoic lithos...

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中国科学院地质与地球物理研究所2.07学术年会

作者： S.HU~1.M.FU~1.S.YANG~2.Y.YUAN~1.& J.WANG~1.1.{1. of Geology and Geophysics,Chinese Academy of Sciences,{1. 1.002.,{2. 2.2. Offshore Oil Exploration and Development Research Centre, CNOOC,1.002.,{1.,china

The palaeotemperature recorded by vitrinite reflectance(R) in the pre-Cenozoic uplifted stratigraphic strata,and in Palaeozoic-Mesozoic remnant basins outside the Cenozoic depocentres,has not been overprinted by later thermal events in the eastern North china Craton(NCC).Based on downhole R data from the Palaeozoic and the Mesozoic subsections, we reconstruct the temperature gradients when the subsections reached their maximum palaeotemperatures in the Middle Triassic and the Cretaceous,and calculate the corresponding heat flow histories since the early *** temperature gradient and heat flow were much higher in the Cretaceous(35-43℃km and 73-83 mW m,respectively) than in the Middle Triassic and at the *** high palaeo-heat flow during the Late Mesozoic implies that the thickness of the"thermal"lithosphere at that time was c.65 km,about half the thickness of c.1.5 km estimated for the Early *** change from a stable thermal regime to an active thermal regime took place during the Late Jurassic-Early Cretaceous(c.1.0 Ma).This tectonothermal event was accompanied by extensive surface erosion,and is also evidenced in the areas adjacent to the NCC,such as the South Yellow Sea and East china Sea *** study provides not only geothermal evidence for the Late Mesozoic lithospheric thinning,but also additional constraints on the thinning mechanism,which is currently being debated.

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改良的小波变换算法在生物微粒检测中的应用(英文)

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生物化学与生物物理进展 2010年第10期37卷 1144-1150页

作者：章永登陈良怡徐涛华中科技大学生命科学与技术学院分子生物物理教育部重点实验室武汉430074 中国科学院生物物理研究所计算与系统生物学中心北京100101

荧光图像的微粒检测已经成为了生物学研究中不可或缺的工具之一.介绍了一种改良的小波变换算法(improved wavelet transform,IWT),该方法实现简单,能够以很高的速度和精度来进行生物微粒的检测.IWT 源自多尺度小波乘积算法(wavelet mult... 详细信息

荧光图像的微粒检测已经成为了生物学研究中不可或缺的工具之一.介绍了一种改良的小波变换算法(improved wavelet transform,IWT),该方法实现简单,能够以很高的速度和精度来进行生物微粒的检测.IWT 源自多尺度小波乘积算法(wavelet multiscale products,WMP),但它不仅解决了 WMP算法遇到的问题,而且在处理各类图像的时候具有更强的适应性.使用人工合成的图像和真实的图像来定量地分析 IWT、WMP以及多尺度方差稳定变换算法(multiscale variance stabilizing transform,MSVST)的检测效果.实验结果表明,IWT 在大多数情况下的检测效果比 WMP 好很多,且与更为复杂的MSVST 算法相当.此外,在处理相同图像时,IWT 的速度比 MSVST 快 2.%.因此,IWT 算法能够普遍适用于各种生物微粒的自动化检测,其简单准确的特点使之成为荧光图像分析更好的选择.

关键词：微粒检测 IWT WMP MSVST

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THE INFLUENCE OF STIMULATING SENSORY-MOTOR CORTEX ON THE ACTIVITY OF NEURONS IN NUCLEUS RAPHE MAGNUS IN THE RATS

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针刺研究 1989年第Z1期 32-33页

作者： Zou Ting Liu Xiang institute of Acupuncture and Moxibustion,{1. Academy of Traditional Chinese Medicine, {2.

The purpose of the work was to study whether SM was involved in the descending inhibitory effects of NRM on nociceptive response. The cxperiments were carried out on rats. Results were as follows: 1. The influence of single and short train stimulating SM or motor areal(MI) on 55 neurons of NRM were observed. Of 55 neurons, 2. units showedthe excitatory response to noxious stimulation, 1.0}units the inhibitory respon-se and 1. units without obvious changes. During stimulation of SM with sin-gle and short train pulse for 55 neurons or MI for 1. neurons, a few unitswere activated (1./55 or 2.1.), only one unit 'were inhibited, most of themshowed no obvious changes.

关键词： NRM SM THE INFLUENCE OF STIMULATING SENSORY-MOTOR CORTEX ON THE ACTIVITY OF NEURONS IN NUCLEUS RAPHE MAGNUS IN THE RATS

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Analyses on Climate Characteristics in Xingtai City during 1.54 - 2.1.

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Meteorological and Environmental Research 2011年第11期2卷 23-24,28页

作者： LIU Jun1.CHENG Xiao-hui2.HUANG Rong3,4 1.Observation and Network Department,Hebei Meteorological Bureau,Shijiazhuang 05002.,china 2.Xingtai Meteorological Bureau in Hebei Province,Xingtai 054000,china 3.{2. institute of Urban Meteorology,china Meteorological Administration,{2. 1.0089,china 4.china Academy of Meteorological Sciences,{2. 1.0081.china

[Objective] The research aimed to analyze climate characteristics in Xingtai City during 1.54-2.1..[Method] Based on climate data in Xingtai City during 1.54-2.1.,by using line chart,trend chart,climatic variability and statistical diagram,the climate characteristics in Xingtai City in 57 years were analyzed.[Result] The annual average temperature,annual average maximum and minimum temperatures in Xingtai City during 1.54-2.1. all presented gradual rise *** rise velocity of annual average maximum temperature was the *** annual average temperature presented rise trend year by *** rise velocity of annual average minimum temperature was the *** annual average rainfall,wind speed,relative humidity and sunshine hours all presented gradual decline trend,while the annual average total cloud amount presented increase *** annual average ground temperatures at 0,5,1.,1. and 2. cm in Xingtai City during 1.54-2.1. were respectively 1..6,1..7,1..8,1..8 and 1..8 ℃.The annual average ground temperatures at 40 and 80 cm,1.6 and 3.2.m in Xingtai City during 1.81.2.1. were respectively 1..2.1..2.1..4 and 1..4 ℃.It was clear that the ground temperature at deep layer gradually rose as depth increase.[Conclusion] The research provided theoretical basis for guiding agricultural production in Xingtai City.

关键词： Xingtai City Climate characteristics Analysis china

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Net photosynthesis rate and transpiration rate of Algonquin in different growth years

Net photosynthesis rate and transpiration rate of Algonquin ...

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ⅩⅪ International Grassland Congress and the Ⅷ International Rangeland Congress

作者： L.J.Xu~1.B.Wang~2.Q.Z.Sun~1.1.rassland Research {1. of Chinese Academy of Agriculture Science Hohhot 01.01. {1. 2.School of Soil and Water Conservation {2. Forestry University {2. 1.0083 {1.

＜正＞Introduction Algonquin is tolerant to drought ***,it can not survive more than 5 years in Chifeng city of Inner *** photosynthesis rate（Pn） and the transpiration rate（Tr） of plants’ leave are the major driver of its *** study was designed to compare Pn and Tr of Algonquin in different years to better understand the suitability of Algonquin on this site.

关键词： Algonquin net photosynthesis rate transpiration rate

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Neuronatin基因研究进展

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生物化学与生物物理进展 2003年第1期30卷 3-6页

作者：余利红周仁平张成岗军事医学科学院放射医学研究所北京100850

Neuronatin (Nnat)是最近克隆的脑特异表达的新基因 ,在大脑发育过程中被选择性地剪接 .人Nnat基因全长 3 973bp ,含有 3个外显子和 2.内含子 .NnatmRNA有α和β两种剪接形式 ,分别编码 81.和 5 4个氨基酸 ,两者具有同一相位的开放阅... 详细信息

Neuronatin (Nnat)是最近克隆的脑特异表达的新基因 ,在大脑发育过程中被选择性地剪接 .人Nnat基因全长 3 973bp ,含有 3个外显子和 2.内含子 .NnatmRNA有α和β两种剪接形式 ,分别编码 81.和 5 4个氨基酸 ,两者具有同一相位的开放阅读框架 ,差别在于 β形式中间的外显子被剪切掉 .人Nnat是单拷贝基因 ,定位于染色体 2.0q1. 2. 1.0}.小鼠Nnat基因定位于 2.染色体末端 .该基因是印记基因 (imprintedgene) ,仅在父本来源的等位基因表达 ,而母本来源的等位基因则被甲基化不能表达 .该基因在胚胎期及出生后早期大量表达 ,而在成年和衰老动物大脑中表达明显降低 ,因此推测该基因与大脑的发育和分化密切相关 .成年动物中垂体前叶是NnatmRNA唯一强表达的地方 .推测其蛋白质产物具有疏水N端和亲水C端 ,是跨膜蛋白 ,其确切功能尚属未知 .

关键词： Neuronatin基因脑发育印记基因研究进展

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SHEEC食管癌细胞中NGAL基因的功能

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生物化学与生物物理学报 2003年第3期35卷 247-254页

作者：李恩民许丽艳蔡唯佳熊华淇沈忠英曾毅汕头大学医学院生物化学与分子生物学教研室汕头大学医学院肿瘤病理研究室中国预防医学科学院病毒学研究所汕头51.031 汕头51.031 北京1.0052

中性粒细胞明胶酶相关脂质运载蛋白 (neutrophilgelatinase associatedlipocalin ,NGAL)是脂质运载蛋白(lipocalin)家族的一个新成员 ,可能是人类的一种新的癌基因 ,但是在肿瘤中的功能不清楚。以往研究发现NGAL基因在SHEEC食管癌细胞... 详细信息

中性粒细胞明胶酶相关脂质运载蛋白 (neutrophilgelatinase associatedlipocalin ,NGAL)是脂质运载蛋白(lipocalin)家族的一个新成员 ,可能是人类的一种新的癌基因 ,但是在肿瘤中的功能不清楚。以往研究发现NGAL基因在SHEEC食管癌细胞中显著过表达 ,表明该细胞是一种用来揭示NGAL基因在肿瘤中功能的良好模型。采用反义封闭技术 ,同时结合裸鼠成瘤实验等研究了反义封闭NGAL基因转录对SHEEC食管癌细胞的浸润和分裂增殖等行为的影响。结果发现 ,反义封闭NGAL基因转录不但可以有效地降低SHEEC细胞分泌的基质金属蛋白酶 9和基质金属蛋白酶 2.活性 ,而与此同时裸鼠成瘤细胞的浸润行为也相应地受到了明显抑制 ,然而SHEEC细胞端粒的长度、拓扑异构酶II的含量以及细胞增殖指数等未发生明显变化。表明NGAL基因在食管癌细胞SHEEC中的功能可能主要是通过明胶酶在促进肿瘤细胞的浸润中发挥作用 ,而可能与肿瘤细胞分裂增殖的相关性不明显

关键词： NGAL基因食管癌细胞肿瘤细胞浸润明胶酶裸鼠成瘤实验

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Internet环境下海量地形数据发布和三维显示

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同济大学学报（自然科学版） 2004年第12期32卷 1671-1676页

作者：张立强童小华杨崇俊刘冬林中国科学院遥感应用研究所北京100101 同济大学测量与国土信息工程系上海200092

论述了在Internet环境下海量地形数据发布和三维显示的关键技术 ,包括平均插值小波建立金字塔数据结构的原理和方法、快速压缩解压缩技术、网络模型和基于视相关的二叉三角形简化地形的算法 .在此基础上 ,开发了基于Internet的全球三维... 详细信息

论述了在Internet环境下海量地形数据发布和三维显示的关键技术 ,包括平均插值小波建立金字塔数据结构的原理和方法、快速压缩解压缩技术、网络模型和基于视相关的二叉三角形简化地形的算法 .在此基础上 ,开发了基于Internet的全球三维可视化信息系统 .实验表明 ,所提出的技术方案是切实可行的 ,完全能够满足Internet环境下大规模数字高程模型和影像实时无缝漫游的要求 .

关键词：地形数据平均插值小波二叉三角形负载平衡分布式组件

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Evaluation of 1. plant barcodes in Bryophyta (Mosses)

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Journal of Systematics and Evolution 2010年第1期48卷 36-46页

作者： 1.2.Yan LIU 3 Hai-Fei YAN 4 Tong CAO 3 Xue-Jun GE 1.{1. of Applied Ecology, Chinese Academy of Sciences, Shenyang 1.001.,{1.) 2.Graduate University of the Chinese Academy of Sciences, {2. 1.0049,{1.) 3(Key Laboratory of Plant Resources Conservation and Sustainable Utilization, South{1. Botanical Garden, Chinese Academy of Sciences, Guangzhou 51.650,{1.) 4(College of Life & Environment Sciences, Shanghai Normal University, Shanghai 2.02.4,{1.) (institute of Applied Ecology Chinese Academy of Sciences Shenyang 110016 China) (Graduate University of the Chinese Academy of Sciences Beijing 100049 China) (Key Laboratory of Plant Resources Conservation and Sustainable Utilization South China Botanical Garden Chinese Academy of Sciences Guangzhou 510650 China) (College of Life & Environment Sciences Shanghai Normal University Shanghai 200234 China) *Correspondence: Tong CAO Xue-Jun GE *Correspondence: *?Author for correspondence: E-mail: ct1946@*** xjge@*** Tel./Fax: 86-2.-372. 2.51.

DNA barcoding is a molecular tool that uses a standardized DNA region to identify *** preliminary study reported here is the first attempt to specifically focus on universality and attributes of candidate barcodes across a wide systematic range of *** tested eight previously proposed plant barcoding regions(atpF-atpH, ITS2. matK, psbK-psbI, rbcL, rpoB, rpoC1. and trnH-psbA) and two popular phylogenetic markers(rps4 and trnL-trnF of cpDNA) in 49 moss species and 9 liverwort species, representing half of the orders in moss *** ITS2. rbcL, rpoC1. rps4, trnH-psbA and trnL-trnF regions showed good universality, and therefore the efficacy of these loci as DNA barcodes was further evaluated in 36 mosses and 2.liverworts, each of which included two to three individuals per *** five loci, ***, rpoC1. rps4, trnH-psbA and trnL-trnF, were easy to amplify and sequence and showed significant inter-specific genetic variability, making them potentially useful DNA barcodes for *** best performing single loci were the rbcL and rpoC1.coding *** loci showed equivalent performance and combinations of them did not greatly increase their discrimination *** addition, phylogenies generated from each of the separate regions and multi-locus combinations by using best-fit and Kimura 2.parameter models were compared, but no significant difference was found.

关键词： Bryophyta DNA barcoding rbcL rpoC1 rps4 trnH-psbA trnL-trnF.

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