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Expressed sequence tag analysis and cloning of trehalose-6-phosphate synthase gene from marine alga Laminaria japonica(Phaeophyta)

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Acta Oceanologica Sinica 2012年第6期31卷 139-148页

作者： XUAN Jinsong 1,4,FENG Yanbin 1,WENG Manli 1,ZHAO Ge 1,SHI Jinfeng 1,YAO Jianting 2.WANG Xiuliang 2.GUO Baotai 3.QIAO Lixian 3.DUAN Delin 2.WANG Bin 1 1 The State Key Laboratory of Plant Genomics,{3. of Genetics and developmental Biology,{2. {3. of sciences,beijing 100101,China 2.The State Key Laboratory of Marine Biology,{3. of Oceanology,{2. {3. of sciences,Qingdao 2.6071,China 3.College of Life sciences,Qingdao agricultural University,Qingdao 2.6109,China 4 Department of Biological Science and Engineering,School of Chemical and Biological Engineering,University of Science and Technology beijing,beijing 100083.China 1. The State Key Laboratory of Plant Genomics Institute of Genetics and Developmental Biology Chinese Academy of Sciences Beijing 100101 China 4. Department of Biological Science and Engineering School of Chemical and Biological Engineering University of Science and Technology Beijing Beijing 100083 China 2. The State Key Laboratory of Marine Biology Institute of Oceanology Chinese Academy of Sciences Qingdao 266071 China 3. College of Life sciences Qingdao Agricultural University Qingdao 266109 China

A high quality cDNA library was constructed from the brown alga Laminaria japonica,with the titer of 1.2.10 5 pfu/*** average insert size of the cDNA library is about 1.6 *** the cDNA library,591 cDNA clones were randomly selected and *** a result,574 EST(expressed sequence tag) sequences were *** of the 574 ESTs were submitted to the dbEST database section of GenBank with the accession numbers from CX942.2. to *** cDNA library was screened with a α-3.0}p labeled 453.bp T P S gene probe,which is a partial sequence yielded from Porphyra *** positive cDNA clones were screened and the sequencing data showed that these four cDNA clones covered majority of *** TPS cDNA *** PCR amplification,sequencing and assembling,the entire ORF(open reading frame) sequence of the T P S gene was obtained,which was named *** encodes a protein containing 908 amino acids with a calculated molecular mass of 101 674 *** LjTPS gene was successfully expressed in *** and *** LjTPS gene has potential application both in plant breeding to stress tolerance and in deciphering the T P S gene function and mechanism to stress tolerance.

关键词： cDNA library comparative study expressed sequence tag(EST) analysis Laminaria japonica trehalose-6-phosphate synthase(TPS) T P S gene

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Effect of Nitrate on Root development and Nitrogen Uptake of Suaeda physophora Under NaCl Salinity

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Pedosphere 2010年第4期20卷 536-544页

作者： YUAN Jun-Feng FENG Gu MA Hai-Yan TIAN Chang-Yan Key Laboratory of Oasis Ecology and Desert environment Xinjiang Institute of Ecology and Geography ChineseAcademy of Sciences Urumqi 830011 （China） Graduate-UniversitY of chinese academy of {2. Beijing 100039 （China） College of Resources and environmental sciences China Agricultural University Beijing 100094 （China）

The effects of NaCl salinity and NO^-3.on growth, root morphology, and nitrogen uptake of a halophyte Suaeda physophora were evaluated in a factorial experiment with four concentrations of NaCl （1, 150, 3.0, and 450 mmol L^-1） and three NO^-3.levels （0.05, 5, and 10 mmol L^-1） in solution culture for 3. d. Addition of NO^-3.at 10 mmol L^-1 significantly improved the shoot （P 〈 0.001） and root （P 〈 0.001） growth and the promotive effect of NO^-3.was more pronounced on root dry weight despite the high NaCl concentration in the culture solution, leading to a significant increase in the root：shoot ratio （P 〈 0.01）. Lateral root length, but not primary root length, considerably increased with increasing NaCl salinity and NO^-3.levels （P 〈 0.001）, implying that Na^＋ and NO3.- in the culture solution simultaneously stimulated lateral root growth. Concentrations of Na^＋ in plant tissues were also significantly increased by higher NaCl treatments （P 〈 0.001）. At 10 mmol L^-1 NO^-3. the concentrations of NO^-3.and total nitrogen and nitrate reductase activities in the roots were remarkably reduced by increasing salinity （P 〈 0.001）, but were unaffected in the shoots. The results indicated that the fine lateral root development and effective nitrogen uptake of the shoots might contribute to high salt tolerance of S. physophora under adequate NO^-3.supply.

关键词： halophyte lateral roots nitrate reductase activity root morphology

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Cloning and sequencing of nifBHDKENX genes of Paenibacillus massiliensis T7 and its nif promoter analysis

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Science China(Life sciences) 2006年第2期49卷 115-122页

作者： ZHAO Hongxin1,2.3. XIE Baoen2.& CHEN Sanfeng1,2.1. State Key Laboratory for {3.biotechnology and Biological School, China agricultural University, {2. 100094, China 2. Key Laboratory for {3.-Microbial Resource and Application of Agriculture Ministry, China agricultural University, {2. 100094, China 3. Chemical and Biological School, ShenYang Normal University, Shenyang 11003., China 1. State Key Laboratory for agrobiotechnology and Biological School China Agricultural University Beijing 100094 China 2. Key Laboratory for agro-Microbial Resource and Application of Agriculture Ministry China Agricultural University Beijing 100094 China 3. Chemical and Biological School ShenYang Normal University Shenyang 110034 China

A 3.0} bp of nifH fragment was PCR amplified from Paenibacillus massiliensis T7 using the universal degenerate primers. The PCR-amplified nifH fragment was labeled with DIG; then used as a probe in Southern blot analysis. Southern blot result showed that there were two positive signals, indicating that there might be two copies of nifH in P. massiliensis T7. A total of 102.4 bp DNA sequence containing purD; nifBHDKENX was obtained by five rounds of inverse-PCR amplification. The predicted proteins of nifBHDKENX had high homology with those from other nitrogen-fixing bacteria. Only one putative σ[54]-dependent promoter sequence was detected upstream of the nifB gene; nifBHDKENX were likely to be organized in one operon. Assays of β-galactosidase activity of P. massiliensis T7PB carrying a nifB-lacZ fusion under different concentrations of NH4 [+]; O2.showed that the expression of nifB-lacZ was strongly inhibited by O2.

关键词： Paenibacillus massiliensis T7, nifBHDKENX, nifB-lacZ, inverse-PCR.

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Greenhouse Gas Emissions from Northeast China Rice Fields in Fallow Season

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Pedosphere 2007年第5期17卷 630-638页

作者： LIANG Wei SHI Yi ZHANG Hua YUE Jin HUANG Guo-Hong institute of Applied Ecology Chinese Academy of Sciences Shenyang 110016 （China） Graduate School of the chinese academy of {2. Beijing 10005g （China） College of Resources and environment Huazhong Agricultural University Wuhan 430070 （China）

CH4, N2. and CO2.emissions from northeast chinese rice fields were measured in the fallow season （November to March） to investigate the effects of freezing-thawing on the emissions. Both CH4 emission from and atmospheric CH4 oxidation by the soil occurred, but the flux was small. During the fallow season, rice fields acted as a minor source of atmospheric CH4, which accounted for about 1% of the CH4 emission during the rice growing period. The field was also a substantial source of atmospheric N2., which ranged between 40 to 77 mg m-2.and eu=counted for 40%-50% of the annual N2. emission. The largest N2. flux was observed in the thawing period during the fallow season. Laboratory incubation tests showed that the largest N2. flux came from the release of N2. trapped in frozen soil. Tillage and rice straw application （either mulched on the soil surface or incorporated in the soil） stimulated the CH4 and CO2.emissions during the fallow season, but only straw application stimulated N2. emission substantially.

关键词： CH4 CO2 freezing-thawing N2. rice field

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Distribution and Migration of Heavy Metals in Undisturbed Forest Soils:A High Resolution Sampling Method

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Pedosphere 2008年第3期18卷 386-393页

作者： RUAN Xin-Ling ZHANG Gan-Lin NI Liu-Jian HE Yue State Key Laboratory of Soil and sustainable Agriculture Institute of Soil Science chinese academy of {2. Nanjing 210008 （China） Graduate School of the chinese academy of {2. Beijing 100049 （China） College of Resources and environmental sciences Nanjing Agricultural University Nanjing 210095 （China）

The vertical distribution and migration of Cu,Zn,Pb,and Cd in two forest soil profiles near an industrial emission source were investigated using a high resolution sampling method together with reference element ***-meter soil profile was sectioned horizontally at 2.cm intervals in the first 40 cm,5 cm intervals in the next 40 cm,and 10 cm intervals in the last 2. *** migration distance and rate of heavy metals in the soil profiles were calculated according to their relative concentrations in the profiles,as calibrated by the reference element *** enrichment of heavy metals appeared in the uppermost layer of the forest soil,and the soil heavy metal concentrations decreased down the profile until reaching their background *** calculated average migration rates of Cd,Cu,Pb,and Zn were 0.70,0.3.,0.3.,and 0.76 cm year-1,respectively,which were comparable to other methods.A simulation model was proposed,which could well describe the distribution of Cu,Zn,Pb,and Cd in natural forest soils.

关键词： distribution heavy metals high resolution sampling method migration rate

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Effects of Terracing and agroforestry on Soil and Water Loss in Hilly Areas of the Sichuan Basin, China

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Journal of Mountain Science 2008年第3期5卷 241-248页

作者： J.H. ZHANG Z.A. SU G.C. LIU Key Laboratory of Mountain Hazards and Earth Surface Processes Chinese Academy of Sciences 610041 China institute of Mountain Hazards and environment Chinese Academy of Sciences ＆Ministry of Water Conservancy Chengdu 610041 China The Graduate School of the chinese academy of {2. Beijing 100049 China

Soil erosion in hilly areas of the Sichuan Basin is a serious concern over sustainable crop production and sound ecosystem. A 3.year experiment was conducted using the method of runoff plots to examine the effects of terracing and agroforestry in farmland systems on soil and water conservation of slope fields in the hilly areas in Jianyang County, Sichuan Province, Southwestern China. A power function (Y = aXb) can statistically describe the relationship between water runoff (Y) and rainfall (X). The regression equation for the treatment of sloping terraces with crops (Plot 2. is remarkably different from that for the treatment of sloping terraces with grasses and trees (Plot 1) and the conventional up- and down-slope crop system (Plot 3. regarding equation coefficients, while regression equations are similar between Plot 1 and Plot 3. Water runoff amount and runoff coefficient of slope fields increased by 2..5～41.0 % and 2..5～69.7 % respectively, compared to those of sloping terraces, suggesting that terracing notably reduced the water runoff in the field. In the case of sloping terraces, lower amount of water runoff was observed on sloping terraces with crops than on sloping terraces with grasses and trees. Sediment yields on the slope fields in the normal year of rainfall distribution were notably higher (3..41～3.1.67 % and 3..06～403.44 % for Plot 1 and Plot 2. respectively) than those on sloping terraces, implying that terracing also plays a significant role in the reduction in soil erosion. It is suggested that terracing with crops is significantly effective for soil and water conservation in cultivatedfarmland, while the conventional practice of up- and down- slope cultivation creates high rates of water runoff and soil sediment transport. Terracing with grasses and fruit trees shows a less reduction in water runoff than terracing with crops, which was observed in the 3.year experiments.

关键词： agroforestry sediment transport runoff sloping terrace soil and water conservation

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RECENT ADVANCES IN SPACE STUDIES OF THE EATRH SURFACE, METEOROLOGY AND CLIMAfE IN CHINA

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空间科学学报 1998年第S1期18卷 61-70页

作者： LU Da-ren(institute of Atmospheric Physics, The chinese {2. of{3., beijing 10002.)

During past 2.years, chinese scientists have conducted a series of scientific,technical, and application research relating to earth observation for satellite and payload development, meteorological and {2.al monitoring, disaster reduction, resources surveying, and {3. development of national economy and society. An short and incomplete review is given in this paper.

关键词： chinese space research Earth observation Satellite programs Remote sensing

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“九五”我国农业科技发展基本思路与建议

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中国农业科学 1996年第1期 1-7页

作者： Science and Technology Management Departmentchinese academy of {2.{3. (chinese academy of {2.{3. beijing 100081) 中国农业科学院科技管理局中国农业科学院北京 100081

对我国改革开放以来,农业和农业科技取得的成就与问题,进行了全面总结。在充分调查分析国内外有关背景材料的基础上,提出了“九五”我国农业科技6大研究目标和14个优先发展重点项目及农业科技发展战略措施。为国家、部门的宏观决策,指... 详细信息

对我国改革开放以来,农业和农业科技取得的成就与问题,进行了全面总结。在充分调查分析国内外有关背景材料的基础上,提出了“九五”我国农业科技6大研究目标和14个优先发展重点项目及农业科技发展战略措施。为国家、部门的宏观决策,指导农业科技工作提供依据。

关键词： “九五” 农业科技发展思路建议

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Single-molecule studies of DNA by molecular combing

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Progress in Natural Science:Materials International 2007年第5期17卷 493-499页

作者： Liu Yuying, Wang Pengye and Dou Shuoxing (College of Science, China agricultural University, beijing 100083. China Laboratory of Soft Matter Physics, beijing National Laboratory for Condensed Matter Physics,{3. of Physics, chinese academy of sciences, beijing 100080, China) [a]College of Science China Agricultural University Beijing China [b]Laboratory of Soft Matter Physics Beijing National Laboratory for Condensed Matter PhysicsInstitute of Physics Chinese Academy of Sciences Beijing China

Molecular combing is a powerful method for aligning a large array of DMA molecules onto a surface. It is a process whereby DNA molecules are stretched and aligned on a glass surface by the force via fluid flow. The ability to comb up to several hundred DNAs on a single cover slip allows for a statistically significant number of measurements to be made. These features make molecular combing an attractive tool for genomic studies, such as DNA replication, DNA transcription, DNA-protein interaction and so on. In this review article, we discuss the molecular combing principle, method and its applications.

关键词： DNA molecule molecular combing DNA-protein interaction.

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Characterization, gene cloning and expression of new xylanase XYNB with high specific activity

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chinese Science Bulletin 2003年第8期48卷 761-765页

作者： ZHANG Honglian, YAO Bin, WANG Yaru, ZHANG Wangzhao & YUAN Tiezheng Feed Research institute, chinese academy of agricultural sciences,{2., China Correspondence should be addressed to Yao Bin(email: yao-bin@***) Feed Research institute Chinese Academy of Agricultural Sciences China

Extracellular xylanase XYNB from Streptomy-ces olivaceoviridis A1 has been purified and characterized. The optimal pH value and temperature of XYNB for its ac-tivity are 5.2.and 60℃, respectively. The specific activity of XYNB is as high as 2.69.78 U/mg. Metal cations, EDTA and SDS have no effects on enzyme activity of XYNB. The gene xynB coding mature protein of XYNB has been cloned by PCR. The forward oligonucleotide primer used in the PCR reaction was synthesized based on the N-terminal amino acid sequence of XYNB mature protein, and the reverse oligonu-cleotide primers are random oligonucleotide. The cloned gene xynB is 576 bp long and its G+C content is 64.3.. The xynB encodes 191 amino acid residues, and the putative mo-lecular weight of XYNB is 2..83. kD. The xynB has been expressed in E. coli, and the expressed xylanase has normal bioactivity.

关键词：基因克隆技术基因表达木聚糖酶 XYNB 比活度

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