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Invasive mechanism and management strategy of Bemisia tabaci(Gennadius) biotype B:Progress report of 973.Program on invasive alien species in China

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Science China(Life sciences) 2009年第1期52卷 88-95页

作者： WAN FangHao1,ZHANG GuiFen1,LIU ShuSheng2.LUO Chen3.CHU Dong4,ZHANG YouJun4,ZANG LianSheng2.JIU Min2.Lü ZhiChuang1,CUI XuHong1,ZHANG LiPing4,ZHANG Fan3.ZHANG QingWen5,LIU WanXue1,LIANG Pei5,LEI ZhongRen1 & ZHANG YongJun1 1 State Key Laboratory for Biology of Plant Diseases and Insect Pests,{3. of Plant Protection,chinese Acadeny of Agriculture {2.,beijing 100094,China 2.{3. of Applied Entomology,Zhejiang University,Hangzhou 3.002.,China 3.{3. of Plant and environment Protection,beijing {13. of agricultural and Forestry {2.,beijing 100089,China 4 {3. of Vegetables and Flowers,chinese Acadeny of Agricullare {2.,beijing 100081,China 5 Department of Entomology,China Agriculture University,beijing 100094,China 1. State Key Laboratory for Biology of Plant Diseases and Insect Pests Institute of Plant Protection Chinese Acadeny of Agriculture Sciences Beijing 100094 China 2. institute of Applied Entomology Zhejiang University Hangzhou 310029 China 3. institute of Plant and environment Protection Beijing Academy of Agricultural and Forestry Sciences Beijing 100089 China 4. institute of Vegetables and Flowers Chinese Acadeny of Agricullare Sciences Beijing 100081 China 5. Department of Entomology China Agriculture University Beijing 100094 China

Bemisia tabaci(Gennadius) biotype B,called a "superbug",is one of the most harmful biotypes of this species complex *** this report,the invasive mechanism and management of *** biotype B,based on our 5-year studies,are *** *** biotypes,B,Q,ZHJ1,ZHJ2.ZHJ3.and FJ1,have been identified in *** B dominates the other biotypes in many regions of the *** diversity in biotype B might be induced by host plant,geographical conditions,and/or insecticidal *** activities of CarE(carboxylesterase) and GSTs(glutathione-S-transferase) in biotype B reared on cucumber and squash were greater than on other host plants,which might have increased its resistance to *** higher activities of detoxification enzymes in biotype B might be induced by the secondary metabolites in host *** adaptive ability of biotype B adults to adverse conditions might be linked to the expression of heat shock protein *** indigenous *** biotypes were displaced by the biotype B within 2.5 *** asymmetric mating interactions and mutualism between biotype B and begomoviruses via its host plants speed up widespread invasion and displacement of other *** biotype B displaced Trialeurodes vaporariorum(Westwood) after 4-7 generations under glasshouse *** adaptive ability of the biotype B to adverse conditions and its rapid population increase might be the reasons of its successful displacement of *** ability of the biotype B to switch to different host plants may enrich its host plants,which might enable it to better compete with *** predatory natural enemies possess greater ability to suppress *** under field *** kairomones in the 3.d and 4th instars of biotype B may provide an important stimulus in host searching and location by its *** present results provide useful information in explaining the mechanisms of genetic diversity,e

关键词： National Basic Research and development Program of China(973 Program) invasive alien species Bemisia tabaci biotype B ecological mechanisms genetic diversity sustainable management

A STUDY ON FIELD WATER CIRCULATION PATTERN IN THE DRYLAND OF NORTHERN CHINA

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Journal of Geographical sciences 1997年第4期7卷 36-43页

作者： Zhong Zhaozhan Zhao Jubao institute of agrometeorology,{2.{3. People’s Republic of China

Based on the observed data of soil moisture from locating experiments from 1986 to 1990, the pattern of field water circulation in dryland of northern China, where the mean annual precipitation is 3.0 600 mm, is studied in this paper using the method of water balance. The results show that water satisfying ratio of spring seeding crops is 83.7 90.8 percent and that of winter wheat is about 70 percent in these areas; about 80 90 percent of water consumption of spring seeding crops and about 60 70 percent of water consumption of winter wheat comes from precipitation during the growing period, the rest comes from the soil water storage before the seeding period. But the available soil water is not used thoroughly, about 3. 70 percent of available soil water remains unused when the crops are harvested. At the fallow period, the amount of soil water lost by evaporation is very important, which takes up 57 68 percent of precipitation in winter wheat field and 73. 2.4 percent in field of spring seeding crops. Thus restraining soil evaporation, raising the storage ratio of natural precipitation and the soil water utilization efficiency of crops, strengthening the circulation ability of soil water by adopting efficient measures of agricultural techniques, are the main ways for exploiting and developing the potential productivity of natural precipitation in these areas.

关键词： crop field water soil evaporation crop water consumption field water circulation dryland

Functional Analysis of the ZAG2.Promoter from Maize in Transgenic Tobaccos

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Journal of Integrative Agriculture 2012年第8期11卷 1266-1273页

作者： LU Min-hui WANG Guo-ying MENG Zheng WANG Jian-hua Plant Genetic and Breeding China Agricultural University Beijing 100193 P.R.China institute of Crop Sciecnes Chinese Academy of Agricultural Sciences Beijing 100081 P.R.China Center for Signal Transduction and Metabolomics Institute of Botany Chinese Academy of Sciences Beijing 100093 P.R.China

The function of the 3.040 bp sequence at the upstream translation starting site （ATG） of the ZAG2.gene, isolated from the maize genome, was studied. The sequence analysis showed that the sequence contained a typical class C MADS-box gene regulatory element. The 5′ UTR region of the gene contains a 1 2.9-bp intron that might have important regulatory functions. To study the sequence function, deletion derivatives of promoter-reporter （uidA） gene fusions were generated and transformed into tobaccos. The GUS staining and fluorescence quantification results showed that the GUS activity was detected only in the third and fourth whorl floral organs of the transgenic tobaccos under driving the promoter including the first intron, while detected in all the organs and was stronger under driving the promoter without the first intron. However, the GUS activity was just detected in one whorl of the fourth or third floral organs under driving of the 3.S promoter. These results suggested that the first intron of the ZAG2.gene contains functional regulatory elements, which turned out to be important for gene expression in the heterologous systems. Moreover, the GUS activity was decreased when the reporter gene driven by the promoters with 5′-deletions, respectively, from -1 606 to -951 and -951 to -42. nts, which indicates that positive regulatory elements are present in these two sequence stretches.

关键词： maize MADS-box gene ZAG2 intron

Inheritance of Resistance to SMV3.and Identification of RAPD Marker Linked to the Resistant Gene in Soybean

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agricultural sciences in China 2002年第3期1卷 245-250页

作者： ZHENG Cui-ming, CHANG Ru-zhen and QIU Li-juan(institute of Crop Germplasm Resources , chinese academy of agricultural sciences ,{2. ) institute of Crop Germplasm Resources Chinese Academy of Agricultural Sciences Beijing 100081

One SMV resistant soybean line (95-53.3. was crossed with four susceptible soybean varieties/ line (HB1, Tiefeng2., Amsoy, Williams) and one resistant introduced line PI4863.5. Their F, and F2.individuals were identified for SMV resistance by inoculation with SMV3. The results showed that in the four crosses of resistant X susceptible, F1 were susceptible and the ratio of F2.populations was 1 resistant : 3.susceptible (mosaic and necrosis), indicating that 95-53.3.carries one recessive gene that confer resistance to SMV3. There is segregation of susceptibility in F2.progenies from the cross of 95-53.3.X PI4863.5, indicating that the SMV3.resistant gene in 95-53.3.is located at different locus from PI4863.5. By bulked segregating analysis (BSA) in F2.populations of 95-53.3.X HB1, one codominant RAPD marker OPN11980/1070 closely linked to SMV3.resistance gene amplified with RAPD primer OPN11 was identified. The DNA fragment OPNll980 was amplified in resistant parent 95-53.3.and resistant bulk, and OPN111070 was amplified in susceptible parent HB1 and susceptible bulk. OPNll980/1070 was amplified in F,. Identification of the markers in F2.plants showed that the codominant marker OPNll980/1070 is closely linked to the SMV resistance locus in 95-53.3. with genetic distance of 2.1cM.

关键词： Soybean SMV RAPD marker Inheritance of resistance

Seasonal influence on the development of parthenotes and cloned embryos in pigs

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Progress in Natural Science:Materials International 2007年第10期17卷 1172-1176页

作者： Zhang Kun1, Zhang Yunhai3.4, Pan Dengke5, Ma Yufang1, Wei Hengxi1, Zhang Jian6, Li Xuyang7, Wang Shaohua1, Li Yan2. Dai Yunping1,2.and Li Ning1(1. State Key Laboratory for agrobiotechnology, College of Biological Science, China {3. University, {2. 100094, China 2. JiPulin Biotech Ltd., {2. 100094, China 3. Danish {3. of {3. sciences, 883. Tjele, Denmark 4. College of Animal Science and Technology, Anhui {3. University, Hefei 2.003., China 5. {3. of Animal sciences, chinese academy of agricultural sciences, {2. 100094, China 6. Animal Clinical Hospital, China {3. University, {2. 100094, China 7. Hebei Minghui Group of Pigs Breeding, Sanhe 0652.0, China) State Key Laboratory for agrobiotechnology College of Biological Science China Agricultural University Beijing 100094 China JiPulin Biotech Ltd. Beijing 100094 China Danish institute of agricultural {2. 8830 Tjele Denmark College of Animal Science and Technology Anhui Agricultural University Hefei 230036 China institute of Animal sciences Chinese Academy of Agricultural Sciences Beijing 100094 China Animal Clinical Hospital China Agricultural University Beijing 100094 China 7. Hebei Minghui Group of Pigs Breeding Sanhe 065200 China

Seasonal influence on the developmental ability of porcine embryos was studied by comparisons of the number of cumulus-oocytes-complexes (COCs) per ovary pair and meiotic maturation ability of oocytes in different seasons; and by the observations on developmental competence of the parthenogenetic oocytes and cloned embryos collected from different seasons. We found that the number of COCs per ovary pairs was significantly higher in spring compared to the other seasons (Pdevelopmental competence of porcine embryos.

关键词： pig cloned parthenogenetic season

Study on the Relationship Between the Ploidy Level of Microspore-Derived Plants and the Number of Chloroplast in Stomatal Guard Cells in Brassica oleracea

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agricultural sciences in China 2009年第8期8卷 939-946页

作者： YUAN Su-xia,LIU Yu-mei,FANG Zhi-yuan,YANG Li-mei,ZHUANG Mu,ZHANG Yang-yong and SUN Pei-tian institute of Vegetables and Flowers,chinese {2. of{3. sciences,beijing 100081,P.R.China [a]institute of Vegetables and Flowers Chinese Academy of Agricultural Sciences Beijing 100081 P. R. China

The relationship between the ploidy level of microspore-derived plants and chloroplast number in stomatal guard cells was studied in cabbage, broccoli, and chinese kale. In the experiment, distribution statistics analysis and t-test were used to perform statistical analysis on chloroplast number of different ploidy level in those stomatal guard cells mentioned above, and morphology identifying and chromosome counting were used to test accuracy of counting chloroplast number in stomatal guard cells. The chloroplast average number in stomatal guard cells was very similar among the different leaf positions on the same plant and among significantly among the different ploidy the different locations in the same stoma in the same variety. All the leaf, while the chloroplast number varied distributions of the chloroplast number in different ploidy stoma were normal distribution fitted. A correlation has been established between ploidy and chloroplast number in the stomatal guard cells. In every single stoma of microspore-derived plants, the chloroplast number for a haploid should not be more than 10, diploids 11 to 15, and polyploids more than 15. The accuracy of this method for identification of different ploidy plants was 93.93.. Furthermore, the accuracy of this method was reliable and did not vary with the plants growth conditions. Therefore, the chromosome ploidy of plants derived from microspore culture in cabbage, broccoli, and chinese kale can be identified by simply counting the chloroplast number in stomatal guard cells.

关键词： Brassica oleracea stomatal guard cells chloroplast number identification of ploidy

The new interpretation of support vector machines on statistical learning theory

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Science China Mathematics 2010年第1期53卷 151-164页

作者： ZHANG ChunHua 1 , TIAN YingJie 2.& DENG NaiYang 3.1 School of Information, Renmin University of China, beijing 100872. China 2.Research Center on Fictitious Economy and Data Science, chinese {2. of {3., beijing 100080, China 3.College of Science, China agricultural University, beijing 100083. China 1. School of Information Renmin University of China Beijing 100872 China2. Research Center on Fictitious Economy and Data Science Chinese Academy of Sciences Beijing 100080 China3. College of Science China Agricultural University Beijing 100083 China

This paper is concerned with the theoretical foundation of support vector machines (SVMs). The purpose is to develop further an exact relationship between SVMs and the statistical learning theory (SLT). As a representative, the standard C-support vector classification (C-SVC) is considered here. More precisely, we show that the decision function obtained by C-SVC is just one of the decision functions obtained by solving the optimization problem derived directly from the structural risk minimization principle. In addition, an interesting meaning of the parameter C in C-SVC is given by showing that C corresponds to the size of the decision function candidate set in the structural risk minimization principle.

关键词： C-support vector classification the minimization principle of the structural risk KKT conditions

Analysis of developmental Proteome at Egg Stage of Drone Honeybees (A. m. ligustica)

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agricultural sciences in China 2010年第3期9卷 392-400页

作者： FANG Yu and LI Jian-ke institute of Apicultural Research, chinese academy of agricultural sciences/Key Laboratory of Pollinating Insect Biology, {2. 100093. P.R.China [a] institute of Apicultural Research Chinese Academy of Agricultural Sciences/Key Laboratory of Pollinating Insect Biology Beijing 100093 P.R.China

An investigation on the proteome of drone egg development of native Italian bee （Apis mellifera ligustica Spinola,1806） was carried out in order to prove up the characteristics in protein expression and regulation at egg stage and open out the molecular mechanism of the development. The experiment was carried out by two-dimensional gel electrophoresis. The results showed that there were 2.0, 2.2.and 2.3.proteins in a wide rang of molecular weight （12.42.169.60 kDa） and in a relatively narrow scope of pI （4.50-9.00） detected on day 1, day 2.and day 3. respectively, during the developmental process of the drone egg. Meanwhile, 164 protein spots were resolved at all the images （i.e., the protein was consistently expressed） along with the egg development, among which 7 were significantly up-expressed （P 〈 0.05） and 4 were significantly down-expressed （P 〈 0.05） while 79 had no significant differences （P 〉 0.05）. In addition, the specific proteins expressing proteins on day 1, day 2.and day 3.were 11, 18 and 18, respectively. Besides, 17 proteins expressed both on day 1 and day 2.but silenced on day 3. and 43.proteins expressed both on day 2.and day 3.but silenced on day 1, while only 8 proteins expressed both on day 1 and day 3.but silenced on day 2. The results indicate that 2.d-old eggs are at the most active expressional stage in the development of drone egg. The protein expressing at all images suggests that it should be indispensable for drone egg development, but their expression pattern is different. The proteins expressing at a specific age of egg suggest that specific proteins are needed in different developmental stages to regulate. And there are more house-keeping proteins in the developmental process of the drone egg than that of worker egg, and it will provide more targets for gene improvement.

关键词： honeybees drone egg two-dimensional gel electrophoresis proteome

Expression pattern and core region analysis of AtMPK3.promoter in response to environmental stresses

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Science China(Life sciences) 2010年第11期53卷 1315-1321页

作者： GAO Fei1,2. SU Qi1, FAN YunLiu1& WANG Lei1 1Biotechnology Research institute/National Key Facility for Crop Gene Resources and Genetic Improvement, chinese {2. of {3. Science, beijing 100081, China 2.ollege of Life and environmental sciences, Minzu University of China, beijing 100081, China 1. Biotechnology Research institute/National Key Facility for Crop Gene Resources and Genetic Improvement Chinese Academy of Agricultural Science Beijing 100081 China2. College of Life and Environmental Sciences Minzu University of China Beijing 100081 China

The protein kinase AtMPK3.a component of the MAP kinase cascade,plays an important role in stress signal transduction in plant cells. To clarify how AtMPK3.is regulated at the transcriptional level in response to various environmental factors, the 1016-bp promoter sequence upstream of the transcription start site of the AtMPK3.gene was isolated. Analyses of the promoter sequence using plant promoter databases revealed that the AtMPK3.promoter contains many potential cis-acting elements involved in environmental stress responses. We constructed four deletion mutants of the AtMPK3.promoter, and introduced the intact and truncated promoter sequences fused to the β-glucuronidase (GUS) gene into Arabidopsis. GUS histochemical staining and quantitative fluorometric GUS assays were performed to visualize and compare the expression patterns in response to different environmental stimuli. The region between-188 and-6{3.upstream of the transcription start site was identified as the essential DNA sequence of the AtMPK3.promoter for responses to drought, high salinity, low temperature, and wounding. These results advance our understanding of the molecular mechanisms controlling AtMPK3.expression in response to different environmental stimuli.

关键词： Arabidopsis thaliana AtMPK3 promoter stress