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Observation of Surface Structure of retinoblastoma Cell Line

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眼科学报 1997年第3期13卷 133-136,140页

作者： Yongping Li, Guanguang Feng, Yuzhen Yi, Jianliang ZhengZhongshan Ophthalmic center, Sun Yat-sen University of Medical sciences, {2. 510060, china Zhongshan Ophthalmic center Sun Yat-sen University of Medical Sciences Guangzhou 510060 China

Objective: We study the adhesion moleclues on the surface of SO-Rb50 retinoblastoma cell *** : The distribution of proteoglycans in the retinoblastoma SO-Rb50 cell line was analyzed by histochem-electron microscopy, using Colloidal Iron in combination with a series of enzyme digestions. In addition, immunohistochemistry was performed using a panel of specific antibodies including neuron specific enolase(NSE) ,glial fibrillary acidic protein(GFAP) , S-100 protein, fibronectin, laminin, and collagen ***: Immunohistochemical stains showed the most marked cytoplasmic reactivity of SO-Rb50 cells with anti-NSE and anti-S100. The cells member and surface was postive with anti-NSE. No reactivity was noted with antibodies against laminin, GFAP, and collagen IV. After incubated with colloidal iron solution, three types of colloidal iron-positive stained material could be distinguished based on difiierencens in shape, size, electron density: (1) electron dense particles, (2. the larger colloidal

关键词：蛋白多糖眼癌癌细胞表面结构

A Population-based Study of Lens Opactities

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Eye Science 1996年第3期16卷 115-117页

作者： Jingjing Xu, Qiang Yu, Siping Zhu, Qing LiuZhongshan Ophthalmic center, Sun Yat-sen University of Medical sciences,{2. 510060,china Zhongshan Opthalmic center Sun Yat-sen University of Medical Sciences Guangzhou China.

Purpose: Age-related lens opacity is the main cause of vision disablitity in elderly population all over the world. This study was designed to investigate the prevalence and incidence of lens ***: 1817 eyes of 932.adults over 45 year-old were sampled randomly from 4 villeges within Doumen, a county located in the south of Guangdong provience, china. The procedure of the examination was performed by ophthalmologists. The lens opacities were graded according to a standarized photo of the Lens Opacities Classification System II (LOGS- II ).results: The prevalence of lens opacities in nuclear color, nuclear opacity, cortical opacity and subcapsular opacity was 16. 1% , 2..,6% , 30. 3% and 8. 7% , respectively. The frequency of all four types of opacities increased with age. The prevalence of lens opacities in four types was higher in female than that in male. Conclusion: The data we got are important for prevalence of lens opacities of population-based study as well as basic information for

关键词： lens opacities epidemiology

Spatio-temporate Expression of P53, Bcl-2.and Fas in the retina of RD, Rds and C3H Mice

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眼科学报 2000年第3期16卷 158-162页

作者： Ying Chen, Qingjiong ZhangOcular Genetic & Molecular Biology, Zhongshan Ophthalmic center, Sun Yat-sen University of Medical sciences {2. 510060, china Ocular Genetic & Molecular Biology Zhongshan Ophthalmic Center Sun Yat-sen University of Medical Sciences Guangzhou 510060 China

PURPOSE: To investigate the expression status of p53, Fas and bcl-2.in the development of retinal degeneration in C3H, rd and rds mice. METHODS: Expression of p53, Fas and bcl-2.in the retina of rd, rds and C3H mice as well as in normal C3B mice in different periods was examined by immunohistochemical technique. reSULTS: The expression of Fas and p53 was not detected in normal C3B mice. Fas and p53 were expressed in the ganglionic layer in the early stage and then in the inner nuclear layer, while the scale and intense increased in the ganglionic layer. There is no difference of bcl-2.expression between the normal mice and mice with retinal degeneration. CONCLUSION: Fas and p53 may be involved in the retinal cell death in rd, rds, and C3B, but bcl-2.may not. It is unknown why p53 and Fas appeared first in ganglion layer but not in the outer nuclear layer where retinal cells death was noticed in early stage.

关键词：视膜 P53 Bcl Fas 表达视网膜光

Management of Post-traumatic Aniridia with retinal Detachment

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Eye Science 2001年第1期17卷 35-38页

作者： Shibo Tang, Guanting Qiu, Pei Wang, Xiaoling LiangZhongshan Ophthalmic center, Sun Yat-sen University of Medical sciences, {2. 510060, china Zhongshan Ophthalmic center Sun Yat-sen University of Medical Sciences Guangzhou 510060 China

Purpose: To reconstruct the anatomic and functional impairment in patients with post-traumatic aniridia, aphakia, and retinal detachment.Methods: Four patients with unilateral aniridia and aphakia as well as retinal detachment as results of severe eye injuries underwent scleral buckling, vitrectomy, membrane peeling, endolaser photocoagulation, silicone oil or gas temponade, combined with iris diaphragm-IOL implantation.results: All four patients achieved successfully anatomic and functional reconstruction after surgery. During five to 2. months postoperative follow-up, all retinas remained attached. The final visual acuity increased from finger counting to 0.1 ～ 0.3.Conclusions: The combination of vitreoretinal surgery and iris diaphrgm-IOL implantation is an effective method for post-traumatic aniridia, aphakia and traumatic retinal detachment. It could ameliorate photophobia and improve the biocular ***, artificial iris diaphragm implantation could prevent silicone oil-endothelia contact and salvage silicone keratopathy. Eye Science 2.01; 17:35 ～ 38.

关键词：视网膜分离对甲氧基苯甲醛解剖学无晶状体眼眼损伤

Comparison of Trans-scleral Fixation of PMMA and Foldable Intraocular Lens in Children

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Eye Science 2001年第1期17卷 61-64页

作者： Yuping Zou, Zhende Lin, Bo Feng, Shaozhen LiZhongshan Ophthalmic center, Sun Yat-sen University of Medical sciences, {2. 510060, china Zhongshan Ophthalmic center Sun Yat-sen University of Medical Sciences Guangzhou 510060 China

Purpose: To observe the difference of the effects of PMMA and foldable intraocular lenses (IOLs) trans-sclerally fixed in pediatric eyes.Methods: Thirty-two children (43 eyes) who had undergone trans-scleral fixation of IOL were retrospected, of whom 5 children were implanted PMMA IOL in both eyes, 6children were implanted PMMA IOL in one eye and foldable IOL in the other eye, 12.hildren were implanted foldable IOL in one eye and 9 chilrden were implanted PMMA IOL in one eye. Mean age was 5.3 years ( range 2.5 ～ 12.years ). Twelve children had traumatic cataract and the others congenital cataract before lens extraction.results: Foldable group (18 eyes ): Mean follow-up was 12.1 months. Visual acuity (VA): compared with the best corrected VA before IOL fixation, postoperative best corrected VA improved in 16 eyes, remained unchanged in 2.eyes. In 14 eyes, one or two stitches were needed to seal the incision. Complications: Severe anterior chamber reaction was seen in 3 eyes. Intraocular bleeding was found in 3 eyes. IOL decentration was detected in 1 eye. Iris capture of IOL was seen in one eye. PMMA group (2. eyes ):Mean follow-up was 2..3 months. Visual acuity (VA): compared with the best corrected VA before IOL fixation, postoperative best corrected VA improved in 19 eyes,remained unchanged in 5 eyes and got worse in one eye. In 2. eyes, one to three stitches were needed to seal the incision. Complications: Severe anterior chamber reaction was seen in 5 eyes. Intraocular bleeding was found in 4 eyes. IOL decentration was seen in one eye. Iris capture of IOL was seen in 3 eyes. Intraocular pressure elevated in one eye.Conclusion: Our study shows that trans-scleral fixation of IOL is a safe procedure in pediatric eyes. Foldable IOL showed similar effect compared with PMMA IOL in pediatric trans-scleral fixation. Eye Science 2.01; 17:61 ～ 64.

关键词： PMMA 折垒透镜小儿固定方法 IOL

Foldable Lens Explantation and Exchange: The reason and Solution

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Eye Science 2001年第1期17卷 54-56页

作者： Danying Zheng , Zhenpin Zhang , Wenhui Yang , Weirong ChenZhongshan Ophthalmic center, Sun Yat-sen University of Medical sciences , {2., 510060, china Zhongshan Ophthalmic center Sun Yat-sen University of Medical Sciences Guangzhou 510060 China

Objective: To report the explantation and exchange of Hydrophilic Acrylic foldable intraocular lens (IOL) on 14 patients who had visual disturbances caused by the change of transparence on optic.Methods: Sixteen Hydrophilic Acrylic foldable intraocular lenses from 14 patients who presented with decreased visual acuity from 6 months to 1 year after normal phacoemulsification and IOL implantation associated with extensive transparent change on optic of the lens. The lenses were explanted with the bisection technique. All the eyes were reinserted with Acrysof foldable lenses.results: Sixteen lenses were removed successfully and exchanged with the new lens in the capsule. The posterior capsular rupture and vitreous loss were found in the first two cases. One of them had the zonulysis due to the radial tear of the anterior capsule during the enlargement of the capsular opening. The anterior vitrectomy was performed with IOL fixed on the ciliary sulcus. The visual acuity of all the patients improved obviously without posterior complication.Conclusion: Foldable lens explantation with the bisection technique and exchange had a successful outcome with improvement of ocular condition. Eye science 2.01; 17:54 ～56.

关键词：折叠透镜亲水性丙烯酸视觉障碍 IOL 透明度

Cellular response and Membrane Formation on the Surface of Implanted Posterior Chamber Intraocular Lens in Rabbit

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眼科学报 1997年第1期13卷 41-45页

作者： Fangyao Yang, Shaozhen Li, Yezi LiuDepartment of Ophthalmology, Nan fang Hospital, First Military Medical University guangzhou 510515, china Zhangshan Ophthalmic {2., Sun Yat-sen Universily of Medical sciences , guangzhou 510055 , china Department of Ophthalmology Nanfang Hospital First Military Medical University Guangzhou 510515 China.

Purpose: In order to study the cellular response and membrane formation on the surface of intraocular lens after cataract surgery and lens implantation, this study was designed to observe the deposits on the surface of implanted posterior chamber intraocular lens (PC-IOL) for 4,7,15,30 and 90 days in rabbits. Methods : 30 rabbits had undergone ECCE with PC-IOL implantation. PC-IOLs were removed at different stages after operation. 2. PC-IOLs were stained by HE to demonstrate cells on the surface of PC-IOL under light microscopy. 5 PC-IOLs for scanning electron microscopy were observed. 5 membranes were studied by transmission electron ***: On 2. implanted PC-IOLs, the incidence of cell adhesion was 100% . The cells adherent to lens included macrophages,fibroblast-like cells,epithelioid cells,giant cells, ultralarge giant cells and lymphocytes. It was seen that there was a thin proteinaceous film on the surface of the implanted PC-IOLs and the membrane was composed of fibrin collagen

关键词：眼兔后室玻璃体 PC-IOL 灌输细胞反应膜形成

Study on Stereometric Parameters of Optic Nerve Head of Normal, Big-cupped Disk and Glaucomatous Eyes Using Heidelberg retina Tomograph

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眼科学报 2000年第3期16卷 163-167页

作者： Xing liu, Yunlan Ling, Xiaoping Zheng, Jingjing HuangZhongshan Ophthalmic center, Sun Yat-sen University of Medical sciences, {2. 510060, china Zhongshan Ophthalmic center Sun Yat-sen University of Medical Sciences Guangzhou 510060 China

Purpose: To investigate the difference of stereometric parameters of optic nerve head between the normal subjects and patients with big-cupped disk and primary open angle glaucoma (POAG).Methods: Twenty-two cases (44 eyes) of normal subjects, 17 cases (34 eyes) of patients with big-cupped disk and 19 cases (37 eyes) of patients with POAG underwent Heidelberg retina Tomograph (HRT) examination to get topography images and stereometric parameters of optic nerve ***: The stereometric parameters of optic nerve head of the normal, patients with big-cupped disk and POAG were 1) disk area (mm2.: 1. 995± 0. 501, 2. 407±0. 661 and 2. 2.8±0.498; 2. cup area (mm2.: 0.573±0.2.4, 1. 095±0. 673 and 1. 340±0. 516; 3) cup/disk ratio: 0. 2.±0. 095, 0. 42.±0. 176 and 0. 589±0.195; 4) rim area (mm2.: 1.461±0.32., 1.312.0.418 and 0. 905± 0.409; 5)cup volume (mm3): 0. 108±0. 073, 0. 347±0. 346 and 0. 550 ±0. 394; 6) rim volume (mm3): 0. 42.±0. 111, 0. 378±0. 2.5 and 0. 2.4±0. 189; 7) mean cup

关键词：青光眼视网膜图视乳头杯视神经乳头

Preliminary Observation of One-site Combined Trabeculectomy and Phacoemulsification in the Management of Cataract Patients with Drug-controllable Glaucoma

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眼科学报 1999年第1期15卷 38-40页

作者： Yuping Zou, Zhende Lin, Dawei Peng, Shaozhen LiZhongshan Ophthalmic center, Sun Yat-sen University of Medical sciences {2. 510060, china Zhongshan Ophthalmic center Sun Yat-sen University of Medical Sciences Guangzhou 510060 China

Purpose: To evaluate the effect of one-site combined phacoemulsification and trabeculectomy in the management of cataract patients with drug-controllable glaucoma. Methods : Twenty-four cases (2. eyes) of cataract patients with glaucoma whose intraocular pressure could be controlled by drugs were observed. All had undergone one-site combined phacoemulsification and trabeculectomy. Intraocular pressure and visual acuity were ***: The follow-up was 1-2. months. Postoperative visual acuity was 0.4-0.6 in 9 eyes, 0.7-0.9 in 7 eyes and 1.0 or better in 9 eyes. Postoperative intraocular pressure was 2.17±0.764kPa (16.2.±5.73mmHg), 1.83±0.90kPa (13.74 ± 6. 77mmHg) lower than that before surgery (t= 11.89, Pressure after ***:One-site combined phacoemulsification and trabeculectomy is a safe, effective and convenient technique for the management of cataract patients with drug-controllable glaucoma. . Eye Science 1999 ;

关键词： trabeculectomy phacoemulsif ication drug-controllable glaucoma