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Cloning and characterization of interferon stimulated genes Viperin and ISG15,and their promoters from snakehead Channa argus

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Progress in Natural Science:Materials International 2007年第12期17卷 1425-1435页

作者： Jia Weizhang1,2. Zhou Xiuxia1,2. Huang Rong1,2.and Guo Qionglin1(1. Institute of Hydrobiololgy, chinese academy of {2., Wuhan 430072. China 2. graduate university of chinese academy of sciences, beijing 100039, China) Institute of Hydrobiololgy Chinese Academy of Sciences Wuhan 430072 China graduate university of {2. academy of sciences Beijing 100039 China

By suppression subtractive hybridization, rapid amplification of cDNA ends and gene walking methods, interferon stimulated genes (ISGs), Viperin and ISG15, and their promoters have been cloned and characterized from snakehead Channa argus. The Viperin cDNA was found to be 1474 nt and contain an open reading frame (ORF) of 1059 nt that translates into a putative peptide of 352.amino acid (aa). The putative peptide of Viperin shows high identity to that in teleosts and mammals except for the N-terminal 70 aa. The ISG15 cDNA was found to be 758 nt and contain an ORF of 468 nt that translates into a putative peptide of 155 aa. The putative peptide of ISG15 is composed of two tandem repeats of ubiquitin-like (UBL) domains, and a canonical conjugation motif (LRGG) at C-terminal. Viperin and ISG15 promoter regions were characterized by the presence of interferon stimulating response elements (ISRE) and γ-IFN activation sites (GAS). ISRE is a feature of IFN-induced gene promoter and partially overlaps interferon regulatory factor (IRF) 1 and IRF2.recognition sites. GAS is responsible for the γ-IFN mediated transcription. One conserved site for NF-κB was found in the promoter region of Viperin. This is the first report of conservative binding motif for NF-κB in accordance with the consensus sequence (GGGRNNYYCC) among teleost ISG promoters. Moreover, there were also TATA, CAAT and Sp1 transcription factor sites in Viperin and ISG15 promoters. In 5′ untranslated region (UTR), snakehead ISG15 gene contains a single intron, which differs from Viperin gene. The transcripts of Vipeirn and ISG15 mRNA were mainly expressed in head kidney, posterior kidney, spleen and gill. The expression levels in liver were found to increase obviously in response to induction by IFN-inducer poly I∶C.

关键词： interferon interferon stimulated gene (ISG) Viperin ISG15 snakehead (Channa argus).

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Simulations for terrestrial planets formation

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Science China(Physics,Mechanics & Astronomy) 2009年第5期52卷 794-803页

作者： ZHANG Niu 1,2.&JI JiangHui 2.3 1graduate university of {2. academy of sciences,beijing 100049,China 2.urple Mountain Observatory,{2. academy of sciences,Nanjing 2.0008,China 3 National Astronomical Observatories,{2. academy of sciences,{12. 100012.China graduate university of {2. academy of sciences Beijing China Purple Mountain Observatory Chinese Academy of Sciences Nanjing China National Astronomical Observatories Chinese Academy of Sciences Beijing China

In this paper,the formation of terrestrial planets in the late stage of planetary formation is investigated using the two-planet *** that time,the protostar formed for about 3 Ma and the gas disk *** the model,the perturbations from Jupiter and Saturn are *** of the mass of outer planet,and the initial eccentricities and inclinations of embryos and planetesimals are also *** results show that,terrestrial planets are formed in 50 Ma,and the accretion rate is about 60%-80%.In each simulation,3-4 terrestrial planets are formed inside"Jupiter"with masses of 0.15 -3.6M⊕.In the 0.5-4 AU,when the eccentricities of planetesimals are excited,planetesimals are able to accrete material from wide radial *** plenty of water material of the terrestrial planet in the Habitable Zone may be transferred from the farther places by this *** could also happen a few times between two major planets only if the outer planet has a moderate mass and the small terrestrial planet could survive at some resonances over time scale of 10 8 *** one of our simulations,commensurability of the orbital periods of planets is very ***,a librating-circulating 3:2.configuration of mean motion resonance is found.

关键词： astrophysics exoplanet planetary formation n-body simulation

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Terrestrial transect study on driving mechanism of vegetation changes

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Science China Earth sciences 2008年第7期51卷 984-991页

作者： ZHANG YuJin1,2.& ZHOU GuangSheng1 1 State Key Laboratory of Vegetation and Environmental Change, Institute of Botany, chinese academy of {2., beijing 100093, China 2.graduate university of chinese academy of {2., beijing 100049, China State Key Laboratory of Vegetation and Environmental Change Institute of Botany Chinese Academy of Sciences Beijing China graduate university of {2. academy of sciences Beijing China

In terms of chinese climate-vegetation model based on the classification of plant functional types, to- gether with climatic data from 1951 to 1980 and two future climatic scenarios (SRES-A2.and SRES-B2. in China from the highest and the lowest emission scenarios of greenhouse gases, the distribution patterns of vegetation types and their changes along the Northeast China Transect (NECT) and the North-South Transect of Eastern China (NSTEC) were simulated in order to understand the driving mechanisms of vegetation changes under climatic change. The results indicated that the vegetation distribution patterns would change significantly under future climate, and the major factors driving the vegetation changes were water and heat. However, the responses of various vegetation types to the changes in water and heat factors were obviously different. The vegetation changes were more sensi- tive to heat factors than to water factors. Thus, in the future climate warming will significantly affect vegetation distribution patterns.

关键词： driving mechanism vegetation terrestrial transect climatic change plant functional types

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Transient species and its properties of melatonin

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Science China Chemistry 2006年第4期49卷 308-314页

作者： ZHU Hongping1,2. ZHANG Zhaoxia1,2. ZHAO Hongwei1, WANG Wenfeng1 & YAO Side1 1. Shanghai Institute of Applied Physics, chinese academy of {2., Shanghai 2.1800, China 2. graduate university of the chinese academy of {2., beijing 100049, China Shanghai Institute of Applied Physics Chinese Academy of Sciences Shanghai China graduate university of the {2. academy of sciences Beijing China

The photo-physical and photo-chemical properties of melatonin (ML) werestudied by 2.6 nm laser flash photolysis (LFP). ML undergoes photo-ionization to produce radicalcation of ML (ML^(·+))and hydrated electrons (e_(aq)^-), while excited triplet state ~3ML~* is alsoformed during photolysis. The oxidation via one electron transfer from ML to SO_4^(·+) and thetriplet state of riboflavin (~3RF~*) has been also investigated and the rate constants for theprocess have been determined to be 8.0x10^9 and 1.4x10^9 L·mol^(-1)·s^(-1) respectively. Theradical cation of 2.2.'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid)(ABTS^(·+)) scavengingcapacities of melatonin and other antioxidants are compared in this paper.

关键词： melatonin, laser flash photolysis, photoionization, triplet state.

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The influence of moderate ENSO on summer rainfall in eastern China and its comparison with strong ENSO

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chinese Science Bulletin 2008年第5期53卷 791-800页

作者： XUE Feng1 & LIU ChangZheng1,2.1 Institute of Atmospheric Physics, chinese academy of {2., beijing 10002., China 2.graduate university of chinese academy of {2., beijing 100049, China chinese Acad Sci Inst Atmospher Phys Beijing 100029 Peoples R China.

The 6 major ENSO events since 1979 are classified into the strong and moderate ENSO based on in-tensity. The composite analysis is performed to reveal the influence of ENSO on East Asian summer monsoon (EASM) and summer rainfall in eastern China. It is shown that the influence is changed with the seasonal cycle in summer, with a weaker influence in June and a stronger influence in August, in-dicating a long lagged effect of ENSO on EASM. Besides, the circulation and rainfall anomalies caused by the strong ENSO are also stronger with an earlier starting time, while the influence of the moderate ENSO is evident in August. The composite summer rainfall in eastern China for the moderate ENSO exhibits a northern rainfall pattern, which is totally different from the classical ENSO-type rainfall pat-tern. Based on the composite analysis, two moderate ENSO years with a similar intensity (i.e., 1995 and 2.03) are compared. The result shows that, the response of EASM to the moderate ENSO during June and July is, to a certain degree, modulated by the circulation systems in mid-high latitudes of Eurasia and in the Southern Hemisphere, thereby inducing a different rainfall distribution in eastern China. In comparison with the strong ENSO in 1983, it is further revealed that, the strong ENSO plays a dominant role in summer rainfall anomalies in eastern China as well as in controlling the influence of the other factors on EASM. The strong ENSO is therefore different with the moderate ENSO.

关键词：中国东部海洋气象学厄尔尼诺现象东亚季风降雨

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Relationship between surface subsidence factor and mining depth of strip pillar mining

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中国有色金属学会会刊：英文版 2011年第S3期21卷 594-598页

作者： GUO Wen-bing1, 2. HOU Quan-lin 1, ZOU You-feng2.1. College of Earth Science, graduate university of {2. academy of sciences, beijing 100049, China 2. School of Energy Science and Engineering, Henan Polytechnic university, Jiaozuo 454000, China College of Earth Science Graduate University of Chinese Academy of Sciences Beijing 100049 China School of Energy Science and Engineering Henan Polytechnic University Jiaozuo 454000 China

Strip pillar mining is one of the most important technical measures to control mining subsidence in China. Mining depth is one of the most important factors that can affect the mining subsidence in strip pillar mining. Six numerical simulation models were set up to simulate the surface subsidence of strip pillar mining in different mining depths. The mining depth in these six models ranges from 300 to 800 m. The simulated results were compared and analyzed. The formula was established based on the simulated results. It demonstrated that the subsidence of strip pillar mining method is related to mining depth. While the other conditions are the same, the subsidence of strip pillar mining increases with the increase of mining depth by logarithmic relationship.

关键词： subsidence factor strip pillar mining mining depth numerical simulation

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CH_4 uptake flux of Leymus chinensis steppe during rapid growth season in Inner Mongolia,China

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Science China Earth sciences 2010年第7期53卷 977-983页

作者： GENG YuanBo1, LUO GuangQiang1,2.& YUAN GuoFu1 1 Institute of Geographic sciences and Natural Resources Research, chinese {2. of sciences, beijing 100101, China 2.graduate university of chinese {2. of sciences, beijing 100049, China Institute of Geographic sciences and Natural Resources Research Chinese Academy of Sciences Beijing China graduate university of {2. academy of sciences Beijing China

The influence of near ground atmosphere CH4 concentration, soil water content, soil Eh, plants, soil temperature, and pH on CH4 uptake flux of Leymus chinensis steppe during rapid growth season was studied using field in-situ measurement in Xilin River basin of Inner Mongolia, China. The results showed that CH4 uptake flux was positively correlated with near ground atmosphere CH4 concentration and soil Eh but negatively correlated with soil water content. Precipitation may decrease the CH4 flux by increasing the soil water content. The average fluxes of three treatments during the observation period were 1.52.(natural conditions, 1.51 (aboveground plants were excluded), and 2.00 mg m?2.d?1 (all plants were removed), respectively. The difference between the CH4 flux of natural condition treatment and that of removing aboveground plants treatment is small, whereas the flux of removing all plants treatment is larger than the other two treatments. This means that the contribution of aboveground plants to CH4 uptake flux is smaller than that of roots or roots-removing process. CH4 uptake flux is not sensitive to soil temperature and the narrow-ranged soil pH (6.36–7.86). The Leymus chinensis steppe acts as a sink for CH4, and thus more studies should be focused on its affecting factors and related mechanisms.

关键词： CH4 uptake flux Leymus chinensis steppe affecting factors static opaque chamber/gas chromatography method

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Effects of the grain size distribution on the temperature-dependent magnetic susceptibility of magnetite nanoparticles

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Science China Earth sciences 2010年第7期53卷 1071-1078页

作者： ZHAO XiangYu1,2.& LIU QingSong1 1 Paleomagnetism and Geochronology Laboratory (SKL-LE), Institute of Geology and Geophysics, chinese academy of {2., beijing 10002., China 2.graduate university of chinese academy of {2., beijing 100049, China Paleomagnetism and Geochronology Laboratory (SKL-LE) Institute of Geology and Geophysics Chinese Academy of Sciences Beijing China graduate university of {2. academy of sciences Beijing China

Magnetite is an important magnetic remanence carrier in natural samples and therefore is of great interest in paleo-, rock-, and environmental magnetism. The magnetic properties of magnetite depend on many factors, e.g., concentration and grain size distribution (GSD). In this study, we theoretically investigated the temperature-dependent susceptibility (TDS) of magnetite nanoparticles with a lognormal GSD. Results show that the TDS is affected highly by the GSD mainly in three aspects. Firstly, the unblocking process becomes smoother with the increase of distribution width, characterizing as a wider Hopkinson peak on the TDS curve. Secondly, the blocking temperature increases with the increase of the median diameter or/and the distribution width. Thirdly, the maximum susceptibility decreases with the increase of distribution width, and has a logarithmic function relation with the standard deviation of the distribution. As a case study, this model was further applied to the thermal products of the chinese loess/paleosol samples to determine the granulometry of newly-formed magnetite upon heating based on TDS curves. The results demonstrate the fidelity and feasibility of this method to determine the GSD of nano-sized magnetic particles.

关键词： Néel theory temperature dependence of susceptibility grain size distribution

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A 12.bit multichannel ADC for pixel detectors in particle physics and nuclear imaging

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Science China(Technological sciences) 2010年第5期53卷 1208-1214页

作者： WEI Wei1,2.3,WANG Zheng1,3 & ZHAO JingWei1,3 1 Institute of High Energy Physics,chinese academy of {2.,beijing 100049,China 2.graduate university of chinese academy of {2.,beijing 100049,China 3 Key Lab of Nuclear Detection Technology and Nuclear Electronics of chinese academy of {2.,beijing 100049,China Institute of High Energy Physics Chinese Academy of Sciences graduate university of {2. academy of sciences Key Lab of Nuclear Detection Technology and Nuclear Electronics of chinese academy of {2.

Modern pixel detectors in nuclear and particle physics experiments and also in nuclear imaging,starve for highly integrated application specified integrated circuit(ASIC),whereas in China the study of ASIC still stays far away from practical *** lack of ASIC strictly limits the research and development of domestic high energy physics field.A 12.bit multichannel ADC designed for high density readout is introduced as a major candidate for solution.A precise model is discussed and the simulation fully agrees with the model,which indicates a key principle of *** is performed according to the given rule,and novel layout techniques are carried *** results in all aspects are also obtained,showing an excellent real performance,which satisfies the practical requirement.

关键词： application specified integrated circuit high density readout Wilkinson ADC multi-channel ADC

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Modeling and verifying Web services driven by requirements:An ontology-based approach

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Science in China(Series F) 2006年第6期49卷 792-820页

作者： HOU Lishan1,3,JIN ZHi1,2.& WU Budan1,4 1. academy of Mathematics and Systems Science, chinese academy of sciences, beijing 100080, China 2. Institute of Computing Technology, chinese academy of sciences, beijing 100080, China 3. Institute of Software, chinese academy of sciences, beijing 100080, China 4. graduate university of chinese academy of sciences, beijing 100049, China academy of Mathematics and Systems Science Chinese Academy of Sciences Institute of Computing Technology Chinese Academy of Sciences Institute of Software Chinese Academy of Sciences graduate university of {2. academy of sciences

Automatic discovery and composition of Web services is an important research area in Web service technology, in which the specification of Web services is a key issue. This paper presents a Web service capability description framework based on the environment ontology. This framework depicts Web services capability in two aspects： the operable environment and the environment changes resulting from behaviors of the Web service. On the basis of the framework, a requirement-driven Web service composition model has been constructed. This paper brings forward the formalization of Web service interactions with π calculus. And an automatic mechanism converting conceptual capability description to the formal process expression has been built. This kind of formal specification assists in verifying whether the composite Web service model matches the requirement.

关键词： Web service environment ontology automatic composition π calculus formal verification.

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