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Sequence analysis of mRNA polyadenylation signals of rice genes

chinese Science Bulletin 2006年第9期51卷 1069-1077页

作者： LU Ying1, 2. GAO Chenxi1,2.3 & HAN Bin1,3,41. National Center for Gene Research, Shanghai Institutes for Biological sciences, chinese {2. of sciences, Shanghai 2.02.3, china 2. graduate School of the chinese {2. of sciences, Beijing 100039, china 3. Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological sciences, chinese {2. of sciences, Shanghai 2.0032. china National Center for Gene Research Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai 200233 China graduate School of the chinese {2. of sciences Beijing 100039 China Institute of Plant Physiology and Ecology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai 200032 China

The formation of eukaryotic mRNAs involves the cleavage and polyadenylation of pre- mRNAs. To investigate the sequence requirement of putative polyadenylation signals (PASs), poly(A) sites and downstream elements (DUEs) in 3′-end-pro- cessing in rice, we compared expressed sequences tags (ESTs) with poly(A) extremity to full-length cDNA sequences and constructed a database of 12.69 pre- mRNA sequences in ?40―+40 nt surrounding the poly(A) sites, which were from 9953 genes. The al- ternative poly(A) sites were revealed in approxi- mately 2.% of mRNAs. Nearly 80% of pre-mRNAs showed stringent requirement of the YA (CA or UA) at poly (A) sites for polyadenylation. About 7.9% had the AAUAAA signals on ?40―?1 nt upstream of the poly(A) sites. Over 60% of mRNAs probably used the one- or two-base variants of AAUAAA hexamers as their PASs in 3′ fragments. The single-base variants of AAUGAA revealed the high frequency in 11.5% of 3′ fragments. The DUEs were detected in 90% of pre- mRNAs, especially more than half of the pre-mRNAs with multi-base variants of AAUAAA had the DUEs surrounding the poly(A) site. The location of DUE is also important for defining the cleavage site. Al- though most of the rice pre-mRNAs did not contain AAUAAA signal, the existence of downstream ele- ments ensured the efficiency of cleavage-polyade-

关键词：多聚腺苷酸序列分析 mRNA 水稻基因工程

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Human remains and the environment of Early Pleistocene in the Nihewan Basin

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Science china Earth sciences 2004年第5期47卷 437-444页

作者： CAI Baoquan1 & LI Qiang2.1. Department of History, Xiamen university, Fujian 361005, china 2. Institute of Vertebrate Palaeontology and Palaeoanthropology, {2. academy of sciences, Beijing 100044, china Xiamen Univ Dept Hist Fujian 361005 Peoples R China chinese Acad Sci Inst Vertebrate Paleontol & Paleoanthropol Beijing 100044 Peoples R China

A new Early Pleistocene Paleolithic site was found in July 2.01 in the Nihewan Ba-sin, Hebei Province. Totally 500 mammal specimens assigned to 2. species and 5 lithic artifacts were collected. The coexistence of Allophaiomys deucalion, Borsodia chinensis and Yangia tingi provides important evidence of chronology. On the basis of the comparison of mammalian fauna, the date of Paleolithic artifacts is probably earlier than 1.8 MaBP, possibly 2.0 MaBP. This is the earliest evidence of hominid activity found so far in North china. The hominid at that time in the Nihewan Basin lived in an environment of arid grasslands with scattered trees of temperate zone. This discovery is significant to the study of human origin and cultural development.

关键词： human remains, environment, Early Pleistocene, Nihewan.

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The features of gravity field and deep structure in western part of the South Yellow Sea

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chinese Science Bulletin 2000年第8期45卷 759-764页

作者： WANG Qianshen1 & AN Yulin1. Institute of Geophysics, chinese academy of {2., Beijing 100101, china 2. chinese university of Geology, Beijing 100083, china Institute of Geophysics Chinesc Academy of Sciences Beijing China chinese university of Geology Beijing China

The gravity data from the sea region to the west of 12.° E of the South Yellow Sea have been processed and the feature of gravity fields analyzed. An experience relationship formula on the filter wavelength and corresponding depth has been put forward. Combined with the geological and seismic data, the deep tectonic framework and structural characters as well as possible favourable prospecting district for hydrocarbon in the South Yellow Sea have been discussed and analyzed.

关键词： western part of the South Yellow Sea gravity field deep structure.

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Prediction method for October 2.03 solar storm

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Science china(Technological sciences) 2006年第5期49卷 629-640页

作者： XIE Yanqiong1, 2. 3, WEI Fengsi1, FENG Xueshang1 & ZHONG Dingkun1, 2.1. State Key Laboratory of Space Weather, Center for Space Science and Applied Research, chinese academy of {2., Beijing 100080, china 2. graduate School, chinese academy of {2., Beijing 100039, china 3. Institute of Meteorology, PLA university of Science and Technology, Nanjing 2.1101, china State Key Laboratory of Space Weather Center for Space Science Applied Research Chinese Academy of Sciences Beijing 100080 China graduate School Chinese Academy of Sciences Beijing 100039 China Institute of Meteorology PLA University of Science Technology Nanjing 211101 China

Aiming at two intense shock events on October 2. and 2., 2.03, this paper presents a two-step method, which combines synoptic analysis of space weather ——“observing” and quantitative prediction ——“palpating”, and then uses it to test predictions. In the first step of “observing”, on the basis of observations of the solar source surface magnetic field, interplanetary scintillation (IPS) and ACE spacecraft, we find that the propagation of the shocks is asymmetric relative to the normal direction of their solar sources, and the Earth is located near the direction of the fastest speed and the greatest energy of the shocks. As the two fast ejection shock events, the fast explosion of coronal mass of the extremely high temperature, the strong magnetic field, and the high speed background solar wind are also helpful to their rapid propagation. In the second step of “palpating”, we adopt a new membership function of the fast shock events for the ISF method. The predicted results show that for the onset time of the geomagnetic disturbance, the relative errors between the observational and the predicted results are 1.8% and 6.7%; and for the magnetic disturbance magnitude, the relative errors are 4.1% and 3.1%, re- spectively. Furthermore, the comparison among the predicted results of our two-step method with those of five other prevailing methods shows that the two-step method is advantageous. The results tell us that understanding the physical features of shock propagation thoroughly is of great importance in improving the prediction precision.

关键词： solar storm, interplanetary shock, interplanetary scintillation, two-step method, ISF method.

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Pre-irradiation with low-dose ^(12.C^(6+) beam significantly enhances the efficacy of AdCMV-p53 gene therapy in human non-small lung cancer

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Science china(Physics,Mechanics & Astronomy) 2007年第2期50卷 221-230页

作者： LIU Bing1,2.ZHANG Hong1,LI WenJian1,LI Qiang1,ZHOU GuangMing1,XIE Yi1,2.HAO JiFang1,MIN FengLing1,2.ZHOU QingMing1,2.& DUAN Xin1,2. Institute of Modern Physics,chinese academy of {2.,Lanzhou 730000,china 2.graduate university of chinese academy of {2.,Beijing 100039,china Institute of Modern Physics Chinese Academy of Sciences Lanzhou 730000China

The combination of ionizing radiation and gene therapy has been investigated. However, there are very few reports about the combination of heavy-ion irradiation and gene therapy. To determine if the pre-exposure to low-dose heavy ion beam enhances the suppression of AdCMV-p53 on non-small lung cancer (NSLC), the cells pre-irradiated or non-irradiated were infected with 2., 40 MOI of AdCMV-p53. Survival fraction and the relative biology effect (RBE) were determined by clonogenic assay. The results showed that the proportions of p53 positive cells in 12.6+ beam induced AdCMV-p53 infected cells were more than 90%, which were signifi-cantly more than those in γ-ray induced AdCMV-p53 infected cells. The pre-exposure to low-dose 12.6+ beam significantly prevented the G0/G1 arrest and activated G2.M checkpoints. The pre-exposure to 12.6+ beam significantly improved cell to apoptosis. RBEs for the 12.6+ + AdCMV-p53 infection groups were 30%-60%, 2.%-130% and 30%-70% more than those for the 12.6+-irradiated only, AdCMV-p53 infected only, and γ-irradiation induced AdCMVp53 infected groups, respectively. The data suggested that the pre-exposure to low-dose 12.6+ beam significantly promotes exogenous p53 expression in NSLC, and the suppression of AdCMV-p53 gene therapy on NSLC.

关键词： heavy-ion beam gene therapy p53 non-small lung cancer adenoviral vector low dose

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Transverse patterns and spatiotemporal instability of a photorefractive oscillator

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chinese Science Bulletin 1997年第9期42卷 724-729页

作者： ZHUANG Jun and TAN Weihan1. Shanghai Institute of Optics and Fine Mechanics, chinese academy of {2., Shanghai 2.1800, china 2. Shanghai university, Shanghai 2.1800, china Shanghai Institute of Optics and Fine Mechanics Chinese Academy of Sciences Shanghai China Shanghai university Shanghai China

IN time realm,instability and chaos phenomena of a nonlinear optical system have been dis-cussed *** recent years,as much interest was attracted to the transverse ef-fects in nonlinear optics,the study was developed t... 详细信息

关键词： photorefractive oscillator spatiotemporal instability transverse patterns.

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Study on growth and properties of novel γ-LiAlO_2.substrate

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Science china(Technological sciences) 2006年第2期49卷 188-193页

作者： ZOU Jun1,2. ZHOU Shengming1, XU Jun1 & ZHANG Rong3 1. Shanghai Institute of Optics and Fine Mechanics, chinese academy of {2., Shanghai 2.1800, china 2. graduate School of chinese academy of {2., Beijing 100039, china 3. Laboratory of Solid State Microstructures and Department of Physics, Nanjing university, Nanjing 2.0093, china 1. Shanghai Institute of Optics and Fine Mechanics Chinese Academy of Sciences Shanghai 201800 China 2. graduate School of chinese {2. of sciences Beijing 100039 China 3. Laboratory of Solid State Microstructures and Department of Physics Nanjing University Nanjing 210093 China

Transparent γ-LiAlO2.single crystal has been grown by Temperature Gradient technique. The surface of the wafer annealed in O2.atmosphere at 1100°C for 70 h became opaque and Li-poor phase (LiAl5O8); while, that annealed in Li-rich atmosphere kept transparent and smooth. The full-width at half maximum value dropped to 30 arcsecs when the wafer was annealed in Li-rich atmosphere. That annealed in O2.atmosphere increased to 78 arcsec. Compared with absorption spectra, we can conclude that the 196 nm absorption peak was caused by Li vacancies and the 736 nm peak was caused by O vacancies.

关键词： γ-LiAlO2 crystal, scanning electron micrograph (SEM), absorption spectra.

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Strong decays of N~*(1535) in an extended chiral quark model

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Science china(Physics,Mechanics & Astronomy) 2009年第10期52卷 1452-1457页

作者： AN ChunSheng1,2.3 & ZOU BingSong1,2.3 1 Institute of High Energy Physics,chinese academy of {2.,Beijing 100049,china 2.Theoretical Physics Center for Science Facilities,chinese academy of {2.,Beijing 100049,china 3 graduate university of chinese academy of {2.,Beijing 100049,china Institute of High Energy Physics Chinese Academy of Sciences Beijing 100049 China Theoretical Physics Center for Science Facilities Chinese Academy of Sciences Beijing 100049 China graduate university of {2. academy of sciences Beijing 100049 China

The strong decays of the N*(1535) resonance are investigated in an extended chiral quark model by including the low-lying $ qqqq\bar q $ components in addition to the qqq component. The results show that these five-quark components in N*(1535) contribute significantly to the N*(1535) → Nπ and N*(1535) → Nη decays. The contributions to the Nη decay come from both the lowest energy and the next-tolowest energy five-quarks components, while the contributions to the Nπ decay come from only the latter one. Taking these contributions into account, the description for the strong decays of N*(1535) is improved, especially for the puzzling large ratio of the decays to Nη and Nπ.

关键词： N*(1535) strong decays five-quark components

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Effects of diatoms on reproduction and growth of marine copepods

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Progress in Natural Science:Materials International 2004年第10期14卷 1-7页

作者： ZHANG Guangtao 1,2. and SUN Song 1 (1.Key Laboratory of Marine Ecology&Environment sciences,Institute of Oceanology,chinese {2. of sciences,Qingdao2.6071,china 2.graduate School,chinese {2. of sciences,Beijing100039,china) chinese Acad Sci Inst Oceanol Key Lab Marine Ecol & Environm Sci Qingdao 266071 Peoples R China chinese Acad Sci Grad Sch Beijing 100039 Peoples R China

Two hot spots in marine ecology,deleterious effects of diatoms and feeding selectivity of copepods,as well as new progress on these two issues achieved in the recent ten years,are *** two issues are considered correlated *** and their metabolites can induce deleterious effects on growth,reproduction and development of copepods,including increase of mortality and decrease of egg production,hatching and growth *** negative effects,resulting from either chemical toxin or nutritional defi-ciency,can be conquered in natural environments by diverse *** is therefore concluded that deleterious effects of diatoms observed in laboratory or during blooming period are only a special case that accommodation of feeding strategy of copepods is *** under-stand their feeding strategy in natural environments is a prerequisite to explaining the mechanisms of deleterious effects caused by diatoms,and makes it possible to re-evaluate the energy flow in marine ecosystems.

关键词： diatoms copepod feeding strategy reproduction deleterious effects

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Integrated precise orbit determination of Shenzhou IV unmanned spacecraft

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Science china(Technological sciences) 2004年第5期47卷 518-525页

作者： L Congmin1,2. GU Yidong3, LIN Baojun3 & GUO Jiong3 1. Center for Space Science and Applied Research, chinese academy of {2., Beijing 100080, china 2. graduate School of the chinese academy of {2., Beijing 100039, china 3. General Establishment of Space Science and Application, chinese academy of {2., Beijing 100080, china Correspondence should be addressed to L Congmin 1. Center for Space Science and Applied Research Chinese Academy of Sciences 100080 Beijing China 2. graduate School of the chinese {2. of sciences 100039 Beijing China 3. General Establishment of Space Science and Application Chinese Academy of Sciences 100080 Beijing China

Considering the characteristic orbit of Shenzhou IV spacecraft and the advantages and disadvantages of several types of domestic orbit measurements, a scheme of IPOD is determined, which uses GPS, SLR and USB observation data jointly plus precise ionosphere corrections to GPS observation data. IPOD system and mathematic models of OD are introduced. The results of different joint OD are analyzed using true observation data, and show that radial accuracy is better than 2.m.

关键词： Shenzhou IV unmanned spacecraft integrated precise orbit determination GPS SLR USB.

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